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The map label for this gene is brnQ2 [H]

Identifier: 57651230

GI number: 57651230

Start: 335842

End: 337149

Strand: Reverse

Name: brnQ2 [H]

Synonym: SACOL0302

Alternate gene names: 57651230

Gene position: 337149-335842 (Counterclockwise)

Preceding gene: 57651238

Following gene: 57651229

Centisome position: 12.0

GC content: 35.86

Gene sequence:

>1308_bases
TTGAAAAAACAAGTTATTATTTCGGGCCTCATGTTATTTTCACTATTTTTTGGAGCCGGAAATTTAATATTCCCGCCCAT
GCTTGGCCATACAGCGGGTCAAAATATGTGGATTGGTATGCTAGGCTTTGCCCTTACAGGCATATTACTCCCCTTTATTA
CTGTTATTGTTGTTGCATTTTATGATGAAGGTGTTGAAAGTGTAGGCAATCGTATACATCCATGGTTCGGGTTTATTTTT
GCTGTCGTGATTTACATGTCTATCGGAGCATTTTACGGTATTCCACGTGCTGCAAATGTCGCGTACGAAATTGGTACAAG
ACACATTTTACCTGTGCATAACCAATGGACTTTAATTATATTCGCAGCAATCTTTTTTGCCATCGTTTACTGGATTAGTT
TAAATCCATCGAAAATCGTTGATAATTTAGGTAAATTATTAACACCGTTATTACTATTAATGGTCGCTCTATTAAGTATT
GCTGTCATTTTCAACCCTGAATCTGCACTAAGTGCACCTAAGGATAAATATATAACACATCCTTTCATTTCAGGAAGTTT
GGAAGGCTATTTTACAATGGATCTTGTTGCTGCGTTAGCTTTTTCCGTAGTCATTGTCAATGGCTATAAGTTTAAAGGCC
TCACAGATCGCATGAAAATTTTAAAATATGTCTGCTTTTCAGGTCTTATTGCAGCCATATTACTTGGAATGATTTACTTT
GCACTTGCATACGTTGGGGCATCAACAGCTCCAGGAAACTTTAAAGATGGTACAGATATATTGACGTACAACTCATTACG
ATTATTTGGTTCGTTCGGTAACCTCGTATTTGGAATGACGGTTATCCTTGCATGCCTAACAACATGTATAGGACTCGTCA
ATGCTTGCGCCACATTTACTAAGAAACACGTACCTAAGTTTTCTTATAAAATATTCGCACTTATTTTCTCTATCATAGGG
TTCTTATTTACAACACTTGGTTTAGAAATGATTTTAAAAATTGCTGTCCCATTATTGACTTTAATATATCCCGTGTCGAT
TGCACTTGTACTCATATCATTTGCTAACATGTTTAGCACATTCAGATTCAGTTGGGCCTATCGACTCGCAACTGTTATTA
CATTGATTATTTCAATTTTACAAATACTAAATAGTTTCAACTTATTACACGGTGTTATTTTGAAATCGTTTATGATGTTA
CCTTTAGCAGATATCGATTTAGCTTGGCTTGTACCATTCATGCTCTTTGCTATTATCGGTTTCATAATCGATGTATTTAT
ACGCCGTCCGAAACAAGCGACAACTTAA

Upstream 100 bases:

>100_bases
TAGAATCATAGTATTGTCATTTAAAGATAAAGTAAGAACGTTTTTATTTTTCAGATTTTTTAAATTATTATGAATATCTA
GTTTTAGGAAGGAAATTACA

Downstream 100 bases:

>100_bases
TAAATGCTCACTGCCTAGTAATGATTGACCCATCGTTACTAGGCTTTTTTATATGAAATTCATGACTTTTCAGTGATACT
GTCTGTCTATTGATTATTTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIF
AVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYF
ALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIG
FLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMML
PLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT

Sequences:

>Translated_435_residues
MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIF
AVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYF
ALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIG
FLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMML
PLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT
>Mature_435_residues
MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIF
AVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYF
ALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIG
FLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMML
PLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=437, Percent_Identity=34.5537757437071, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48034; Mature: 48034

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]