Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 57650941

GI number: 57650941

Start: 2457224

End: 2458201

Strand: Reverse

Name: cobA [H]

Synonym: SACOL2396

Alternate gene names: 57650941

Gene position: 2458201-2457224 (Counterclockwise)

Preceding gene: 57650942

Following gene: 57650940

Centisome position: 87.5

GC content: 37.22

Gene sequence:

>978_bases
ATGTCTGTAGAGGAATATGGCAAGGTTTACTTGATAGGTGCGGGTCCAGGTAATCCGAATTATTTGACGAAAAAGGCTGA
GCGTTTAATACGTGAAGCGGATGTCATTCTATATGATCGATTAGTGAATCCGTTAATTTTACAATATGCGAATTTGACTA
CTGAAATTATCGATGTTGGTAAAAAGCCGTATGCAAAACATATTCAGCAAGAAAAAATAAATGACTGTATCGTGGAAGCA
GCGCGTCGATATAACAAGGTTGTTAGACTTAAAGGTGGCGATCCAGCGATATTTGGTCGTGTGCAAGAAGAAGTCGACAC
ACTAAATGAACATCATATTGCGTTTGAAATTGTACCAGGGGTGACATCAGCGAGCGCAGCAGTTGCTACTATGCAGACAG
GTTTGACAATGCGTACAGTTGCTAAAAGTGTGACATTTTCTACAGGTCACTTTAAAGATTCAGAAGAAAATGAAGTGGAT
GTCAATTCCTTAGTGAATGGTGGCACGTTAGCAATTTATATGGGGGTAAAACGTTTAGGGAAAATTATCACACAGATACA
ACAATACACAGATATTGATTACCCGATAGCGATTGTCTTTCAAGCATCGTGTTTTAACGAGTTTGTTGTTAAGGGGCGTT
TAAGTAATATTATTTCAAAATTGCAACACTATTCAATTGAGGCGAAACCAGGTATATGTATTATAGGGGAAGTTGTTGAT
TATACTGAAAACACTCCTAAATCATATGATCCTATGAAGCAATTTTATGTAGTAAGTGGTTCTAAACATGACGCCCTTAT
GCTCTGTGAACATTTATATGACGAAGGTTATGGCTGTTTGCTAAACCCAAATGATACATCGAATGGCACATATCATTCGT
CGCAATATGATTACTATGATGCATTTATTAAGCAGCAAGAAAATGTAACATATATTTCAACCGATCGTGCAGATGCTAAT
ACAGTGTTATGTCACTAA

Upstream 100 bases:

>100_bases
ACGATCAAAAAATTGATTTAAATACAGGTATTGTTCAAGAACCTGATGAAGGTTGTGTAGATGTTTATGAGGTAGAAGTT
ACAGACGGGAACGTATATAT

Downstream 100 bases:

>100_bases
TTTATAAAAAATAAATGAATAAGTAAGGTTTCAACCGAGAGAATATATTCGTGTTGAAGCCTTATTTGTCGTTGTGCCAA
ATTTGAACGATTTAGATTGG

Product: uroporphyrinogen III methylase SirB

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEA
ARRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVD
YTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADAN
TVLCH

Sequences:

>Translated_325_residues
MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEA
ARRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVD
YTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADAN
TVLCH
>Mature_324_residues
SVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAA
RRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVDV
NSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVDY
TENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANT
VLCH

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=238, Percent_Identity=36.9747899159664, Blast_Score=141, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322922, Length=263, Percent_Identity=30.4182509505703, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 36336; Mature: 36204

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00840 SUMT_2 ; PS00473 GNRH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVG
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPG
CCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECC
VTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVDVNSLVNGGTLAIYMGVKRLG
CCCHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHH
KIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVD
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHH
YTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYD
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEECCCCHHHH
AFIKQQENVTYISTDRADANTVLCH
HHHHCCCCEEEEECCCCCCCEEECC
>Mature Secondary Structure 
SVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVG
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPG
CCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECC
VTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVDVNSLVNGGTLAIYMGVKRLG
CCCHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHH
KIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVD
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHH
YTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYD
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEECCCCHHHH
AFIKQQENVTYISTDRADANTVLCH
HHHHCCCCEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1906874 [H]