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The map label for this gene is leuA [H]

Identifier: 57650729

GI number: 57650729

Start: 2105979

End: 2107508

Strand: Direct

Name: leuA [H]

Synonym: SACOL2046

Alternate gene names: 57650729

Gene position: 2105979-2107508 (Clockwise)

Preceding gene: 57650728

Following gene: 57650730

Centisome position: 74.96

GC content: 36.27

Gene sequence:

>1530_bases
ATGAGTAGTCATATTCAAATTTTTGATACGACACTAAGAGACGGTGAACAAACACCAGGAGTGAATTTTACTTTTGATGA
ACGCTTGCGTATTGCATTGCAATTAGAAAAATGGGGTGTAGATGTTATTGAAGCTGGATTTCCTGCTTCAAGTACAGGTA
GCTTTAAATCTGTTCAAGCAATTGCACAAACATTAACAACAACGGCTGTATGTGGTTTAGCTAGATGTAAAAAATCTGAC
ATCGATGCTGTATATGAAGCAACAAAAGATGCAGCGAAGCCGGTCGTGCATGTTTTTATAGCAACATCACCTATTCATCT
TGAACATAAACTTAAAATGTCTCAAGAAGACGTTTTAGCATCTATTAAAGAACATGTCACATACGCGAAACAATTATTTG
ACGTTGTTCAATTTTCACCTGAAGATGCAACGCGTACTGAATTACCATTCTTAGTGAAATGTGTACAAACTGCCGTTGAC
GCTGGAGCTACAGTTATTAATATTCCTGATACAGTCGGCTACAGTTACCATGATGAATATGCACATATTTTCAAAACCTT
AACAGAATCTGTAACATCTTCAAATGAAATTATTTATAGTGCTCATTGCCATGACGATTTAGGAATGGCTGTTTCAAATA
GTTTAGCTGCAATTGAAGGCGGTGCGAGACGAATTGAAGGCACTGTAAATGGTATTGGTGAACGAGCAGGTAATGCAGCA
CTTGAAGAAGTCGCGCTTGCACTATACGTTCGAAATGATCATTATGGTGCTCAAACTGCTCTTAATCTCGAAGAAACTAA
AAAAACATCGGATTTAATTTCAAGATATGCAGGTATTCGAGTGCCTAGAAATAAAGCAATTGTTGGCCAAAATGCATTTA
GTCATGAATCAGGTATTCACCAAGATGGCGTATTAAAACATCGTGAAACATATGAAATTATGACACCTCAACTTGTTGGT
GTAAGCACGACTGAACTTCCATTAGGAAAATTATCTGGTAAACACGCCTTCTCAGAGAAGTTAAAAGCATTAGGTTATGA
CATTGATAAAGAAGCGCAAATAGATTTATTTAAACAATTCAAGGCCATTGCGGACAAAAAGAAATCTGTTTCAGATAGAG
ATATTCATGCGATTATTCAAGGTTCTGAGCATGAGCATCAAGCACTTTATAAATTGGAAACACTGCAACTACAATATGTC
TCTAGCGGCCTTCAAAGTGCTGTTGTTGTTGTTAAAGATAAAGAGGGTCATATTTACCAGGATTCAAGTATTGGTACTGG
TTCAATCGTAGCAATTTACAATGCAGTTGATCGTATTTTCCAGAAAGAAACAGAATTAATTGATTATCGTATTAATTCTG
TCACTGAAGGTACTGATGCCCAAGCAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATT
GATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGT
AGGTAATTAA

Upstream 100 bases:

>100_bases
AAATTGAAAAAGTTGGTCGTGAATTACGCGAAATGATGCCTTTTATTAAATCTAAAAGCATTGAAAAATAAGATAGACCT
ACAATGAGGAGTTGTTAAAT

Downstream 100 bases:

>100_bases
TTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAA
ATTATAAGTAATAAATATAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSD
IDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVG
VSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYV
SSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
DHDILQASCKAYVEAHAKFAAENVEKVGN

Sequences:

>Translated_509_residues
MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSD
IDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVG
VSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYV
SSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
DHDILQASCKAYVEAHAKFAAENVEKVGN
>Mature_508_residues
SSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDI
DAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDA
GATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAAL
EEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGV
STTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVS
SGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGID
HDILQASCKAYVEAHAKFAAENVEKVGN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=495, Percent_Identity=43.8383838383838, Blast_Score=404, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320019, Length=383, Percent_Identity=27.9373368146214, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=421, Percent_Identity=27.0783847980998, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=534, Percent_Identity=23.5955056179775, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=529, Percent_Identity=22.8733459357278, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55675; Mature: 55544

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQA
CCCCEEEEEEHHCCCCCCCCCCEEHHCCEEEEEEEECCCCEEEECCCCCCCCCCHHHHHH
IAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEHHHHCCCHHHHHH
SIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEY
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
AHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
HHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCHHHHCCCCCEECHHHHHHHHHCCHHH
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIH
HHHHEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQF
CCCCCCCCCHHHCCCCEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
KAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQ
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
DSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCC
DHDILQASCKAYVEAHAKFAAENVEKVGN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQA
CCCEEEEEEHHCCCCCCCCCCEEHHCCEEEEEEEECCCCEEEECCCCCCCCCCHHHHHH
IAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEHHHHCCCHHHHHH
SIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEY
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
AHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
HHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCHHHHCCCCCEECHHHHHHHHHCCHHH
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIH
HHHHEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQF
CCCCCCCCCHHHCCCCEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
KAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQ
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
DSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCC
DHDILQASCKAYVEAHAKFAAENVEKVGN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA