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The map label for this gene is leuB

Identifier: 57650730

GI number: 57650730

Start: 2107511

End: 2108557

Strand: Direct

Name: leuB

Synonym: SACOL2047

Alternate gene names: 57650730

Gene position: 2107511-2108557 (Clockwise)

Preceding gene: 57650729

Following gene: 57650731

Centisome position: 75.02

GC content: 35.72

Gene sequence:

>1047_bases
ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAAT
TATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGC
CTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTGCGTAAATCCTTAAATTTATTTGTAAATATACGCCCCACTAC
CGTTGTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGGAAGAACGCGTTGAAGGCACAGATTTAGTTATAGTCCGTGAAT
TGACAAGTGGTATTTATTTTGGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTAACATCAGTTGATAAAGAAAA
TGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAATGAAGTAAGTCAATTATATCCAGAAGTAACAGTAAATCACT
TACTTGTTGATGCTTGTAGTATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTATGTGAAAACTTATTTGGC
GATATTTTAAGTGATGAAGCTTCAGTGATTCCTGGTTCACTTGGTTTATCACCTTCTGCTAGTTTTAGTAACGATGGTCC
AAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATTGCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTAT
CTTTAGCGATGTGTTTACGTGAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAGCATGATT
GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAAATTCTATCTCAAAAATTGAA
TCACTAA

Upstream 100 bases:

>100_bases
TTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTT
GAGAAGGTAGGTAATTAATT

Downstream 100 bases:

>100_bases
GGGGGAGATGTAAATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGCGAACCGC
AACTATTATACATTGATTTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWT
DPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMI
EHGQTTADLGGKLNTTDIFEILSQKLNH

Sequences:

>Translated_348_residues
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWT
DPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMI
EHGQTTADLGGKLNTTDIFEILSQKLNH
>Mature_347_residues
TYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTD
PNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREE
IERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGD
ILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIE
HGQTTADLGGKLNTTDIFEILSQKLNH

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=30.8571428571429, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=29.6296296296296, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=29.2479108635098, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=28.1524926686217, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI28178838, Length=319, Percent_Identity=28.8401253918495, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=34.020618556701, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=47.0254957507082, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=34.8189415041783, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787381, Length=374, Percent_Identity=25.9358288770053, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=335, Percent_Identity=30.4477611940298, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=351, Percent_Identity=29.9145299145299, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=29.5454545454545, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=354, Percent_Identity=28.5310734463277, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=357, Percent_Identity=45.0980392156863, Blast_Score=290, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=33.695652173913, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=34.2776203966006, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=30.1675977653631, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24643270, Length=361, Percent_Identity=33.2409972299169, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=32.7777777777778, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=29.4117647058824, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=28.8235294117647, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=28.8235294117647, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078635, Length=364, Percent_Identity=28.2967032967033, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161078637, Length=364, Percent_Identity=28.2967032967033, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161078633, Length=364, Percent_Identity=28.2967032967033, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24650122, Length=364, Percent_Identity=28.2967032967033, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078639, Length=361, Percent_Identity=28.5318559556787, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI20130355, Length=350, Percent_Identity=22.2857142857143, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_STAA3 (Q2FF66)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_494662.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q2FF66
- SMR:   Q2FF66
- STRING:   Q2FF66
- EnsemblBacteria:   EBSTAT00000036990
- GeneID:   3915080
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_2011
- eggNOG:   COG0473
- GeneTree:   EBGT00050000023966
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38214; Mature: 38083

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 94-94 BINDING 104-104 BINDING 132-132 BINDING 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTL
CCEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCHHHHHH
NACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKE
HHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHEECCCHHCCHHHH
ERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKL
HHCCCCCEEEEEEHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLR
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLNQPDAADELEQHIYSMIEHGQTTADLGGKLNTTDIFEILSQKLNH
HHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTL
CEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCHHHHHH
NACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKE
HHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHEECCCHHCCHHHH
ERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKL
HHCCCCCEEEEEEHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLR
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLNQPDAADELEQHIYSMIEHGQTTADLGGKLNTTDIFEILSQKLNH
HHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA