Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 57650706

GI number: 57650706

Start: 2081386

End: 2082171

Strand: Direct

Name: yafV [C]

Synonym: SACOL2021

Alternate gene names: 57650706

Gene position: 2081386-2082171 (Clockwise)

Preceding gene: 57650705

Following gene: 57650708

Centisome position: 74.09

GC content: 32.06

Gene sequence:

>786_bases
ATGAAAGTCCAAATTTATCAATTACCGATTGTTTTTGGAGATAGTTCTAAAAATGAAACTCAAATAACGCAATGGTTCGA
AAAAAATATGAATGCTGAAGTAGATGTAGTCGTTTTACCTGAAATGTGGAATAATGGCTATGATTTAGAGCATTTAAATG
AAAAAGCAGATAATAATCTAGGACAAAGTTTTTCATTTATTAAACATTTAGCTGAAAAATATAAAGTCGATATCGTAGCT
GGGTCAGTTTCTAACATTAGAAACAATCAAATATTTAATACAGCTTTTAGTGTGAATAAAAGTGGCCAGCTGATTAATGA
ATACGACAAAGTTCATTTAGTTCCTATGTTAAGAGAGCATGAATTTTTAACCGCTGGCGAATACGTAGCTGAACCCTTTC
AATTGTCTGACGGTACTTATGTGACCCAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGT
AGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACG
TGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGA
TAGTTATAAACCCAAATGGAGATTTAGTTGGAGAATTAAATGAATCCGCAGATATTTTGACTGTAGATTTAAACTTAAAT
GAAGTAGAACAGCAACGCGAAAATATACCTGTATTTAAAAGTATTAAATTAGATTTGTATAAATAA

Upstream 100 bases:

>100_bases
GTAGAGCCTAGGACTTTGATTTTATGTCTTGTGCTCGTTTTTTGCGTTTCAACATAACTTTTAGTAAGTTATATATAAGA
TCTATCAAGGATGTGATGTT

Downstream 100 bases:

>100_bases
GAAGCGCCCGAAATAATATTTAACACATGTCATGACTAAACATAGATTTATGAGAGATTTTTCATAAGTCTATGTTTATT
TTTTTATAATAGATAACTCA

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: ORF 5

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVA
GSVSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
EVEQQRENIPVFKSIKLDLYK

Sequences:

>Translated_261_residues
MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVA
GSVSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
EVEQQRENIPVFKSIKLDLYK
>Mature_261_residues
MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVA
GSVSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
EVEQQRENIPVFKSIKLDLYK

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI9910460, Length=257, Percent_Identity=28.4046692607004, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI297632350, Length=185, Percent_Identity=30.2702702702703, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI297632348, Length=185, Percent_Identity=30.2702702702703, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI5031947, Length=185, Percent_Identity=30.2702702702703, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1786412, Length=257, Percent_Identity=33.0739299610895, Blast_Score=120, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17556280, Length=264, Percent_Identity=29.1666666666667, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323383, Length=277, Percent_Identity=29.6028880866426, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322335, Length=302, Percent_Identity=22.8476821192053, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355835, Length=239, Percent_Identity=32.6359832635983, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17933642, Length=258, Percent_Identity=26.7441860465116, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YAG5_STAAU (P55177)

Other databases:

- EMBL:   X52543
- PIR:   S20793
- ProteinModelPortal:   P55177
- InterPro:   IPR003010
- InterPro:   IPR001110
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29826; Mature: 29826

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50263 CN_HYDROLASE; PS01227 UPF0012

Important sites: ACT_SITE 41-41 ACT_SITE 110-110 ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNL
CEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCCCHHHHHHHHCCCC
GQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPMLREH
CHHHHHHHHHHHHHEEEEEECCHHHCCCCEEEEEEEECCCCCCHHHHCCCEEEEEHHHCC
EFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSRLQHW
CCEECCHHHCCCEEECCCCEEEEEHHHHCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHH
HSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCEEEEECCHH
EVEQQRENIPVFKSIKLDLYK
HHHHHHHCCCEEEEEEEEECC
>Mature Secondary Structure
MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNL
CEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCCCHHHHHHHHCCCC
GQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPMLREH
CHHHHHHHHHHHHHEEEEEECCHHHCCCCEEEEEEEECCCCCCHHHHCCCEEEEEHHHCC
EFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSRLQHW
CCEECCHHHCCCEEECCCCEEEEEHHHHCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHH
HSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCEEEEECCHH
EVEQQRENIPVFKSIKLDLYK
HHHHHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7565609