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The map label for this gene is pckA

Identifier: 57650576

GI number: 57650576

Start: 1893131

End: 1894723

Strand: Direct

Name: pckA

Synonym: SACOL1838

Alternate gene names: 57650576

Gene position: 1893131-1894723 (Clockwise)

Preceding gene: 57652080

Following gene: 57650580

Centisome position: 67.39

GC content: 35.09

Gene sequence:

>1593_bases
ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACGTCACATTTTCAACTTTCGAC
GACACAACTTTATAATAAAATCTTAGACAATAACGAAGGGGTATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAA
AATATACTGGTCGTTCGCCTAAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTAGATAAAAAAGATGAACTATA
CGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATGTTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATT
TATTTGCTAAAAATATGTTTATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
GCACCACATTTTAAAGCAGATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATTATTTCATTTAAACACAAAGT
CATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAAAAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGC
AAGATATTATGAGCATGCATTGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
GGTAAAACAACCTTATCGGCTGACCCACACCGTAAACTAATCGGTGATGATGAACACGGCTGGAATAAAAACGGGGTCTT
TAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTTTCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAAT
ATGGTGCAATTTTAGAGAACACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGACAATCGTTATACAGAAAACACG
CGTGCCGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCTAAAGCAGCACATCCAAATACAATTATTTTCTT
AACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAGTTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTT
TCACTTCTAAATTAGCTGGTACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGAGCACCGTTC
TTCCCGTTACACCCTACTGTTTACGCTGATCTATTAGGTGAACTTATCGATTTACATGATGTTGATGTTTATCTTGTTAA
TACTGGATGGACTGGCGGAAAATATGGTGTAGGACGTAGAATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGA
TTTCTGGCAAATTGAAAAATGCAGAATATACAAAAGATAGTACGTTTGGTTTAAGCATTCCTGTAGAAATTGAAGATGTA
CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAAGCAGAAGATTTAATTCAACG
TTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAACATATTGCTGAAAAAGGTAGCTTCAACAAATAA

Upstream 100 bases:

>100_bases
ATAAATTTACAGTAAAATATTGTAGATTAATTATGTTAATGTGTTATACTAATTAAATGTAAAGGCTTACATTTAAATTA
TCGCTTTGGAGGGATTTAGG

Downstream 100 bases:

>100_bases
ATTTGAATACTAAATCAAAACCACCCGTGTGAACGGGTGGTTTGTTCTGCGGCTATAAGCCTTCCTTACTGGCCAGCCCT
AAAAGGGCACTGACAAGTCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEI
NQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENT
RAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPF
FPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK

Sequences:

>Translated_530_residues
MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEI
NQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENT
RAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPF
FPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK
>Mature_529_residues
SVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEIN
QPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSA
PHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTG
KTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTR
AAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFF
PLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVP
KTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=503, Percent_Identity=52.4850894632207, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322950, Length=496, Percent_Identity=49.3951612903226, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_STAA1 (A7X3N3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442366.1
- ProteinModelPortal:   A7X3N3
- SMR:   A7X3N3
- STRING:   A7X3N3
- EnsemblBacteria:   EBSTAT00000004640
- GeneID:   5560263
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1776
- eggNOG:   COG1866
- GeneTree:   EBGT00050000024569
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- BioCyc:   SAUR418127:SAHV_1776-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59378; Mature: 59247

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSP
CCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
KDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTM
CCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCE
LKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKADPEVDGTKSETFVI
EEEEHHHHHHHHHHHHHCEEECCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
ISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
EEECCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILEN
CCEEECCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
TVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISK
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCEEEEEECCCCCCCCCHHH
LNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
VDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
CEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHH
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSP
CCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
KDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTM
CCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCE
LKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKADPEVDGTKSETFVI
EEEEHHHHHHHHHHHHHCEEECCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
ISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
EEECCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILEN
CCEEECCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
TVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISK
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCEEEEEECCCCCCCCCHHH
LNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
VDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
CEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHH
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA