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The map label for this gene is papS [H]

Identifier: 57650412

GI number: 57650412

Start: 1538264

End: 1539466

Strand: Reverse

Name: papS [H]

Synonym: SACOL1497

Alternate gene names: 57650412

Gene position: 1539466-1538264 (Counterclockwise)

Preceding gene: 57650413

Following gene: 57650411

Centisome position: 54.8

GC content: 32.0

Gene sequence:

>1203_bases
ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGTTTTGAAGCATATTATGTAGG
TGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCATGATATAGATATCACAACAAGTGCAACGCCGGATGAAATAG
AATCTATCTTTAGTCATACGATACCTGTAGGTAAAGAACATGGCACGATAAATGTAGTTTTTAATGATGAAAATTATGAA
GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACATTTGTTCGTGATTTATACGA
AGATTTGCAACGACGAGATTTCACGATGAATGCGATAGCAATGGATACAGCATACAAATTGTATGATTATTTTGATGGTC
AACAAGATATTAATAATCGAATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
CGATGTTTAAGGTTCCAGTCACAATTATCATTTGATATTGCAACGGAAACATTCGAAGCGATGCGTATACAAATGGCAGA
TATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTAACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTA
ATCATTTAAAATCGCTGAAAGCATTTAATTATATGCCGTATTTCGAACATCTTGATATGAATCAAATTAATGTAACTGAA
GCAATTGATTTAGAATTGTTGATTGCTATAGTATCAGTTAAATTTGATATTAATTACTCATTGAAGCCTTTAAAGCTAAG
TAACCGACAAGTAAAAGATATCAATCAATATATTCAAATTATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAA
AAATGTTTGTTTATGATTATGATACGCATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTACCTATGCATAATAGAAAAGA
CATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAATGCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAA
TTGAGATAGCGATTGTAACAGGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
TAG

Upstream 100 bases:

>100_bases
ATTGCTGAACGTTTTGGATCAGAACTTATTACAGATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAA
AAGTAAAGGCGAGTAATAAT

Downstream 100 bases:

>100_bases
TCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATATCTGGACAAAGCATTGCGGAATCACTTAATATTT
CACGCACTGCAGTAAAAAAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
AIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDI
QGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Sequences:

>Translated_400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
AIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDI
QGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
>Mature_400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
AIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDI
QGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=223, Percent_Identity=36.322869955157, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI87081691, Length=190, Percent_Identity=34.7368421052632, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=29.3577981651376, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=208, Percent_Identity=35.0961538461538, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321016, Length=209, Percent_Identity=29.6650717703349, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281360112, Length=230, Percent_Identity=33.9130434782609, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357337, Length=230, Percent_Identity=33.9130434782609, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46437; Mature: 46437

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
IPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIA
CCCCCCCCEEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHHEE
MDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEA
HHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
HHHHHHCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHH
AIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDY
HHHHHHHHHHHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLN
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEECCEEEEEEC
AKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
CCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
IPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIA
CCCCCCCCEEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHHEE
MDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEA
HHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
HHHHHHCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHH
AIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDY
HHHHHHHHHHHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLN
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEECCEEEEEEC
AKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
CCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA