Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 57650411

GI number: 57650411

Start: 1537306

End: 1538277

Strand: Reverse

Name: birA [H]

Synonym: SACOL1496

Alternate gene names: 57650411

Gene position: 1538277-1537306 (Counterclockwise)

Preceding gene: 57650412

Following gene: 57650410

Centisome position: 54.75

GC content: 30.35

Gene sequence:

>972_bases
ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATATCTGGACAAAGCATTGCGGA
ATCACTTAATATTTCACGCACTGCAGTAAAAAAAGTGATTGACCAATTAAAGTTAGAGGGATGTAAAATAGATTCAGTAA
ATCATAAAGGGCATTTATTACAACAGCTCCCAGATATTTGGTATCAAGGTATAATAGACCAATATACAAAAAGTTCTGCT
TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAGTCACTTGTTGGAAATCAATC
TTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGTGGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAG
GACTTTGGATGTCTGTCGTGTTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGATATATATATTGATAATGGTAA
AGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGATGGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGA
CGCAACAACTAGAAAACTTTGATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
AGATATCAATTTTTAGAGATATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTAACGTTACCTTTTTCTGAAAT
TCGTGAAGAATATACTGCAGCTTCTAATATTTGGAATAGAACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGAC
AAGCAATTGATTTAGATTACGATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
ATAGATTTTTAA

Upstream 100 bases:

>100_bases
GGTCCGTGGTTAAAAGATGTGCTAAGACAAATTGAGATAGCGATTGTAACAGGTAAAGTAAGCAACGAAGAAACTGAAAT
TTTGAAATGGGTGGATAATC

Downstream 100 bases:

>100_bases
CACTAAAGCAAGGAGAGATAGCTATGGGTATGGCAACCTATGCCGTTGTGGATTTGGAAACAACAGGCAACCAATTAGAT
TTTGACGATATCATTCAAAT

Product: birA bifunctional protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSA
LFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLD
RYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISAD
IDF

Sequences:

>Translated_323_residues
MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSA
LFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLD
RYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISAD
IDF
>Mature_322_residues
SKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSAL
FDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGI
RDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDR
YQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADI
DF

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=291, Percent_Identity=25.085910652921, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37087; Mature: 36956

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLL
CCCHHHHHHHHHHCCCCCEECHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCCCHHHH
QQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGR
HHHHHHHHHHHHHHHHHHHHCEEHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
GRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPND
CCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHEEEEECHHHHHHHCCCCCEEEECCCE
IYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLD
EEEECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCH
RYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDY
HHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECC
DGYLIVRDEAGESHRLISADIDF
CCEEEEECCCCCCCEEEEECCCC
>Mature Secondary Structure 
SKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLL
CCHHHHHHHHHHCCCCCEECHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCCCHHHH
QQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGR
HHHHHHHHHHHHHHHHHHHHCEEHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
GRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPND
CCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHEEEEECHHHHHHHCCCCCEEEECCCE
IYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLD
EEEECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCH
RYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDY
HHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECC
DGYLIVRDEAGESHRLISADIDF
CCEEEEECCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]