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The map label for this gene is ptrB [C]

Identifier: 41410049

GI number: 41410049

Start: 4411265

End: 4413304

Strand: Reverse

Name: ptrB [C]

Synonym: MAP3951c

Alternate gene names: 41410049

Gene position: 4413304-4411265 (Counterclockwise)

Preceding gene: 41410056

Following gene: 41410048

Centisome position: 91.38

GC content: 69.71

Gene sequence:

>2040_bases
ATGACGTCGCACGCCCGCACCGATGCCGCCGCCGAGTACGACCCCTACCTGTGGCTCGAGGACATGACCGGCGAGCAGGC
GCTGGATTGGGTGCGCGCCCGCAACGAGCCGACGCTGGCCCGCTTCCGCGACGCCGAGTTCGAGCGGATGCGCGTCGAGG
CGCTCGAGGTGCTCGACACCGACGCCCGCATCCCGTACGTGGTCCGCCGCGGCGAGCACCTCTACAACTTCTGGCGCGAC
GCCGACAACCCGCGCGGGCTGTGGCGGCGCACCACGCTGGACAGTTACCGCACCGACGCGCCCGAGTGGGAGGTGCTGAT
CGACGTCGACGAGCTGGGCCGCGCCGACGGCGAGAAATGGGTGTGGGCCGGCGCCGCCGTCATCCACCCCGAATACACCC
GCGCGCTGATCAGCTTGTCCCGCGGCGGCTCAGACGCCTGCATCGTGCGTGAATTCGACATGCAGACAAAGCAATTCATC
GACGACGGGTTCGCGCTCGCCGAGGCCAAGTCGCAGCTCAGCTGGGAGGACCCCGACACCGTGCTGGTCGGCACGGACTT
CGGCCCCGGCACCCTCACCGAGTCCGGCTATCCGCGGGTGGTCAAGCGGTGGCGCCGGGGCACGCCGCTGGACGAGGCGC
AGACGGTGTTCGAGGGCGCCCGCTCCGACGTGAACGTGGTGGCCAGCGTCAGCCGCATCCCCGGCTTCGGGCGCACCCTG
ATCGGCCGGGCGCTGGACTTCTGGAACGAAGAGGTCTACGAGCTGCGGGGCGGCCAGCTCGTCCGCATCGACACCCCGAC
CGACGCCAGCGTGAGCCTGCACCGCCGGTGGCTGCTGGTCGAGTTGCGCAGCGACTGGACGCTGGGCACCCGCACCTACG
CCGCGGGCTCGCTGCTGGCCGCCGACTACGAGGAATTCCTTTCCGGCGCACGGGATTTCCGCGTCGTCTTCGAACCCGAC
GAGCACACCGCGCTCAACCACTACGCGTGGACCGAAGACCGGCTGCTGATGGTGACCCTGGTCGACGTGGCCAGCCGCGT
CGAGATCGTCAGACCCGGTGACTGGCGGCGCGAGCCGGTGGCCGGGATCCCGGCCGCGACCAACACCGTCGTCGTCTCCG
CGGACGACACCGGCGACGAATTCTTCCTGGACTCCAGCGGATTCGACTCACCGTCGCGGCTGATGCGGGGCACCGACGAC
GGGCGACTCGAGCAGCTCAAGACCGCCCCCGCCTTCTTCGATGCCGAAAACATCAGCGTGGCACAGTATTTCGTCGACTC
CACCGACGGCACACCGATCCCCTACTTCGTGGTGCGGCCGACCGGCCCGGCAGCCGACCGGCCCGGGCCCACCCTGCTCT
ACGGCTACGGCGGCTTCGAGTCCTCGAACACCCCCGGCTACAGCGGGGTGCTGGGCCGGCTGTGGCTGGCCCGCGGCGGC
ACCTACGTGCTGGCCAACATCCGCGGCGGCGGCGAGTACGGCCCCGGCTGGCACACCCAGGCGATCCGCGAGGGCCGGCA
CAAGGTGCACGAGGACTTCGCCGCTGTGGCAACCGATTTGGTGGACCGCGGCATCACGACGGTCGCCCAACTCGGGGCCC
AGGGCGGCAGCAACGGCGGACTGCTGATGGGCATCATGTTGACCAAGTATCCGGAGAAGTTCGGCGCGCTGGTGTGCAGC
GTGCCGCTGCTGGACATGAAGCGCTACCACCTGCTGCTGGCCGGCGCCTCCTGGGTGGCCGAATACGGCGACCCGGACAA
CCCGGACGACTGGGCGTTCATCTCCGAATACTCGCCGTACCAGAATATTTCGGCGACGCGCCGGTACCCGCCGGTGCTGA
TCACCACCTCCACCCGCGACGACCGGGTGCACCCGGGGCATGCCCGCAAGATGGCGGCCGCCCTGGAGGCCGCCGGCCAC
CGGGTCTACTACTACGAGAACATCGAGGGCGGTCATGCCGGGGCGGCGGACAACGAACAGGTGGCGTTCAAGTCGGCGCT
GACCTATTCGTTCCTGTGGCAGACGCTCGGCCGGCCCTAA

Upstream 100 bases:

>100_bases
CCCCGGCCGAACCCGGACGTGCGAAGACAGTCATCTGCTCTCACTCCTCGTTCACCTGTGTAATACACCTCACACTACCG
CCGCGACCACAATGGCTGGC

Downstream 100 bases:

>100_bases
GATCGTCGACGTGTCGGCGCAACGGATCGAACTCACCCTGCTGGCGACCGGACTGATCTTCATCCTGGTTTCGGCGGCGC
AGGCGCGCTACCGGTTCATC

Product: hypothetical protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MTSHARTDAAAEYDPYLWLEDMTGEQALDWVRARNEPTLARFRDAEFERMRVEALEVLDTDARIPYVVRRGEHLYNFWRD
ADNPRGLWRRTTLDSYRTDAPEWEVLIDVDELGRADGEKWVWAGAAVIHPEYTRALISLSRGGSDACIVREFDMQTKQFI
DDGFALAEAKSQLSWEDPDTVLVGTDFGPGTLTESGYPRVVKRWRRGTPLDEAQTVFEGARSDVNVVASVSRIPGFGRTL
IGRALDFWNEEVYELRGGQLVRIDTPTDASVSLHRRWLLVELRSDWTLGTRTYAAGSLLAADYEEFLSGARDFRVVFEPD
EHTALNHYAWTEDRLLMVTLVDVASRVEIVRPGDWRREPVAGIPAATNTVVVSADDTGDEFFLDSSGFDSPSRLMRGTDD
GRLEQLKTAPAFFDAENISVAQYFVDSTDGTPIPYFVVRPTGPAADRPGPTLLYGYGGFESSNTPGYSGVLGRLWLARGG
TYVLANIRGGGEYGPGWHTQAIREGRHKVHEDFAAVATDLVDRGITTVAQLGAQGGSNGGLLMGIMLTKYPEKFGALVCS
VPLLDMKRYHLLLAGASWVAEYGDPDNPDDWAFISEYSPYQNISATRRYPPVLITTSTRDDRVHPGHARKMAAALEAAGH
RVYYYENIEGGHAGAADNEQVAFKSALTYSFLWQTLGRP

Sequences:

>Translated_679_residues
MTSHARTDAAAEYDPYLWLEDMTGEQALDWVRARNEPTLARFRDAEFERMRVEALEVLDTDARIPYVVRRGEHLYNFWRD
ADNPRGLWRRTTLDSYRTDAPEWEVLIDVDELGRADGEKWVWAGAAVIHPEYTRALISLSRGGSDACIVREFDMQTKQFI
DDGFALAEAKSQLSWEDPDTVLVGTDFGPGTLTESGYPRVVKRWRRGTPLDEAQTVFEGARSDVNVVASVSRIPGFGRTL
IGRALDFWNEEVYELRGGQLVRIDTPTDASVSLHRRWLLVELRSDWTLGTRTYAAGSLLAADYEEFLSGARDFRVVFEPD
EHTALNHYAWTEDRLLMVTLVDVASRVEIVRPGDWRREPVAGIPAATNTVVVSADDTGDEFFLDSSGFDSPSRLMRGTDD
GRLEQLKTAPAFFDAENISVAQYFVDSTDGTPIPYFVVRPTGPAADRPGPTLLYGYGGFESSNTPGYSGVLGRLWLARGG
TYVLANIRGGGEYGPGWHTQAIREGRHKVHEDFAAVATDLVDRGITTVAQLGAQGGSNGGLLMGIMLTKYPEKFGALVCS
VPLLDMKRYHLLLAGASWVAEYGDPDNPDDWAFISEYSPYQNISATRRYPPVLITTSTRDDRVHPGHARKMAAALEAAGH
RVYYYENIEGGHAGAADNEQVAFKSALTYSFLWQTLGRP
>Mature_678_residues
TSHARTDAAAEYDPYLWLEDMTGEQALDWVRARNEPTLARFRDAEFERMRVEALEVLDTDARIPYVVRRGEHLYNFWRDA
DNPRGLWRRTTLDSYRTDAPEWEVLIDVDELGRADGEKWVWAGAAVIHPEYTRALISLSRGGSDACIVREFDMQTKQFID
DGFALAEAKSQLSWEDPDTVLVGTDFGPGTLTESGYPRVVKRWRRGTPLDEAQTVFEGARSDVNVVASVSRIPGFGRTLI
GRALDFWNEEVYELRGGQLVRIDTPTDASVSLHRRWLLVELRSDWTLGTRTYAAGSLLAADYEEFLSGARDFRVVFEPDE
HTALNHYAWTEDRLLMVTLVDVASRVEIVRPGDWRREPVAGIPAATNTVVVSADDTGDEFFLDSSGFDSPSRLMRGTDDG
RLEQLKTAPAFFDAENISVAQYFVDSTDGTPIPYFVVRPTGPAADRPGPTLLYGYGGFESSNTPGYSGVLGRLWLARGGT
YVLANIRGGGEYGPGWHTQAIREGRHKVHEDFAAVATDLVDRGITTVAQLGAQGGSNGGLLMGIMLTKYPEKFGALVCSV
PLLDMKRYHLLLAGASWVAEYGDPDNPDDWAFISEYSPYQNISATRRYPPVLITTSTRDDRVHPGHARKMAAALEAAGHR
VYYYENIEGGHAGAADNEQVAFKSALTYSFLWQTLGRP

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=278, Percent_Identity=34.1726618705036, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1788150, Length=704, Percent_Identity=22.7272727272727, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24583414, Length=319, Percent_Identity=35.1097178683386, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI221510989, Length=278, Percent_Identity=36.6906474820144, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 75610; Mature: 75479

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHARTDAAAEYDPYLWLEDMTGEQALDWVRARNEPTLARFRDAEFERMRVEALEVLDT
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCC
DARIPYVVRRGEHLYNFWRDADNPRGLWRRTTLDSYRTDAPEWEVLIDVDELGRADGEKW
CCCCCCEECCCHHHHHHHHCCCCCCCCEEECCHHHHCCCCCCEEEEEEHHHHCCCCCCEE
VWAGAAVIHPEYTRALISLSRGGSDACIVREFDMQTKQFIDDGFALAEAKSQLSWEDPDT
EEECEEEECHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHCCCCCCCCE
VLVGTDFGPGTLTESGYPRVVKRWRRGTPLDEAQTVFEGARSDVNVVASVSRIPGFGRTL
EEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHEEEHHHCCCCCHHH
IGRALDFWNEEVYELRGGQLVRIDTPTDASVSLHRRWLLVELRSDWTLGTRTYAAGSLLA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCHHHCCCHHHH
ADYEEFLSGARDFRVVFEPDEHTALNHYAWTEDRLLMVTLVDVASRVEIVRPGDWRREPV
HHHHHHHCCCCCEEEEECCCCCCCCCCEECCCCCEEEEEHHHHHHHEEEECCCCCCCCCC
AGIPAATNTVVVSADDTGDEFFLDSSGFDSPSRLMRGTDDGRLEQLKTAPAFFDAENISV
CCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCHHHHHHCCCCEECCCCCEE
AQYFVDSTDGTPIPYFVVRPTGPAADRPGPTLLYGYGGFESSNTPGYSGVLGRLWLARGG
EEEEEECCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
TYVLANIRGGGEYGPGWHTQAIREGRHKVHEDFAAVATDLVDRGITTVAQLGAQGGSNGG
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
LLMGIMLTKYPEKFGALVCSVPLLDMKRYHLLLAGASWVAEYGDPDNPDDWAFISEYSPY
EEEEHHHHHCCHHHHHEEEECCHHCCCEEEEEEECHHHHHHCCCCCCCCCCEEEECCCCC
QNISATRRYPPVLITTSTRDDRVHPGHARKMAAALEAAGHRVYYYENIEGGHAGAADNEQ
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
VAFKSALTYSFLWQTLGRP
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSHARTDAAAEYDPYLWLEDMTGEQALDWVRARNEPTLARFRDAEFERMRVEALEVLDT
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCC
DARIPYVVRRGEHLYNFWRDADNPRGLWRRTTLDSYRTDAPEWEVLIDVDELGRADGEKW
CCCCCCEECCCHHHHHHHHCCCCCCCCEEECCHHHHCCCCCCEEEEEEHHHHCCCCCCEE
VWAGAAVIHPEYTRALISLSRGGSDACIVREFDMQTKQFIDDGFALAEAKSQLSWEDPDT
EEECEEEECHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHCCCCCCCCE
VLVGTDFGPGTLTESGYPRVVKRWRRGTPLDEAQTVFEGARSDVNVVASVSRIPGFGRTL
EEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHEEEHHHCCCCCHHH
IGRALDFWNEEVYELRGGQLVRIDTPTDASVSLHRRWLLVELRSDWTLGTRTYAAGSLLA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCHHHCCCHHHH
ADYEEFLSGARDFRVVFEPDEHTALNHYAWTEDRLLMVTLVDVASRVEIVRPGDWRREPV
HHHHHHHCCCCCEEEEECCCCCCCCCCEECCCCCEEEEEHHHHHHHEEEECCCCCCCCCC
AGIPAATNTVVVSADDTGDEFFLDSSGFDSPSRLMRGTDDGRLEQLKTAPAFFDAENISV
CCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCHHHHHHCCCCEECCCCCEE
AQYFVDSTDGTPIPYFVVRPTGPAADRPGPTLLYGYGGFESSNTPGYSGVLGRLWLARGG
EEEEEECCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
TYVLANIRGGGEYGPGWHTQAIREGRHKVHEDFAAVATDLVDRGITTVAQLGAQGGSNGG
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
LLMGIMLTKYPEKFGALVCSVPLLDMKRYHLLLAGASWVAEYGDPDNPDDWAFISEYSPY
EEEEHHHHHCCHHHHHEEEECCHHCCCEEEEEEECHHHHHHCCCCCCCCCCEEEECCCCC
QNISATRRYPPVLITTSTRDDRVHPGHARKMAAALEAAGHRVYYYENIEGGHAGAADNEQ
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
VAFKSALTYSFLWQTLGRP
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]