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The map label for this gene is fadD35

Identifier: 41408499

GI number: 41408499

Start: 2696650

End: 2698224

Strand: Direct

Name: fadD35

Synonym: MAP2401

Alternate gene names: 41408499

Gene position: 2696650-2698224 (Clockwise)

Preceding gene: 41408498

Following gene: 41408500

Centisome position: 55.83

GC content: 69.46

Gene sequence:

>1575_bases
ATGCCCGGTGATTTCGCGACGGTCGGCGCGACGTTGCGGCACCAGGCGCGGCGGCGCGGCGACCACCCGCTGCTGATCTG
CGACGCCGAACGGATCAGCTACGCCGAGGCCGACGTCCGATCGGCCGAACTCGCCCGGGGGCTGATCGCCCTTGGCGCCG
GCAAGGGAACGCATGTCGGGCTGCTCCACCCGAACGGTGCGCGGTTCGTCGTCGCGATGCTGGCGGCGGCCCGGATCGGC
GCCGTGGTGGTGCCGTTCTCGACGTTCGTCACCGCGCGCGAGCTGCGCGAGCAGCTTCTCGACAGCGACGTCGAGATCCT
GTTGAGCGCCCGCTCCTTTCGCTCGCACGACTATGCGCGGCGCTTGTCCGAGGCGGTGTCGGAGACCGACTTCGATCCCG
GCCGTCGGTTGTTTTGCACCGCGGCGCCGCAATTGCGCCGGGTGCTGTTCGCCCCGCAGACGGTGGGCGCGCCGGGCGGC
GGCATCGACCCGGCACTGCTGGCCGCGATGGAGGACGACGTGCAGGCGTGCGATCCGCTGGCCATCGTGTACACCTCCGG
GTCGACCAGCACCCCCAAGGGGGTGGTGCACACCCACGGTGCGCTGCTGGAACACCAGCGCAACCTCAACGGAATCCGGG
GTTTGACGGCAGACGACCGATTGTTCTGCAATTCACCGTTCTTCTGGATCGGCGGGTTCGCCTTCGGACTGCTGGCCACA
CTGGTGGCCGGATCGACGCTGATATGCTCCAACGCCACCGACGCCGGGGCGACTCTGGACCTGCTGGAAGCCGAAAAGCC
CACCATGACCAACGGTTTCAGCGCGGGGATCGCCCACCTGGCCGAGCACCCGAGCTTTGCCGACCGCGATCTGTCGTCGA
TGCGGCGGGGGAACCTGTATCCGATCATGGCGGTCGAGGCGCGCCCCGCCGACCCCGAACTGCGGCACAACATGCTGGGC
ATGACCGAGGCCGGCGGGGTGGTCCTGATCGGCGACGACGAAGCGGACCAGCCCGAGCACCGGCGCGGCTCGTTCGGCAA
ACCCGCGCCCGGGTTCGAGGCCAGGATCCTCGACCCCGACACCGGGGCCGCCGTCGCGGTGGGTAAGGTGGGCGAACTGT
GCATCCGCGGGCCGTATCTGATGCAGCGGTACCACAAACGGAGCCGGGAAGAGTGTTTCGACCCCGACGGCTGGTTCCAC
ACCGGCGATCTGGTGCGCGCCGACGCCGACGGATACTTCTATTTCGCCGGGCGGCTGGGCGCGATGATCAAGACCGCGGG
CGCCAACGTCTCGGCGGTCGAGGTCGAAAAGGCGATCGCGGCGGTCACCGGCGGCGCGACCGCCTACGTGGTCGCGATTC
CCGATGCCCGGCGCGGCCAGCTGGTCGCCGCCGCGGTGGTGTGGCCGGATGACCGGGCCGCCCTGGATCCGGACGCGCTG
CGCGAACGACTCAAATCCGAACTGTCGGCGTATAAGATCCCCCGGCGGTTCGCTTCCCTGCGCCGCGCCGACGTCCCACT
GCTGTCCAGCGGCAAGGTCGACCTGCGGCAACTGAGGAAGCTGTTCGATGCCTGA

Upstream 100 bases:

>100_bases
ATCGCGATCGTCGACGCCCTGCCCCGAACCCCGTCCGGCAAGGCCGACCTGGCCGCGGTCCGCGGCTACTTCGCCGAGCG
GCCGACGGTACTGCGCAACC

Downstream 100 bases:

>100_bases
GGCGAGCGCACCCACCATCGATCACCTGGTCAGGTCACGCGCCGCCGAGTTCGGCGGCAAGCCGATGGTGATCGACCCGG
GTTCCCGCATCAGCTATGAC

Product: FadD35

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MPGDFATVGATLRHQARRRGDHPLLICDAERISYAEADVRSAELARGLIALGAGKGTHVGLLHPNGARFVVAMLAAARIG
AVVVPFSTFVTARELREQLLDSDVEILLSARSFRSHDYARRLSEAVSETDFDPGRRLFCTAAPQLRRVLFAPQTVGAPGG
GIDPALLAAMEDDVQACDPLAIVYTSGSTSTPKGVVHTHGALLEHQRNLNGIRGLTADDRLFCNSPFFWIGGFAFGLLAT
LVAGSTLICSNATDAGATLDLLEAEKPTMTNGFSAGIAHLAEHPSFADRDLSSMRRGNLYPIMAVEARPADPELRHNMLG
MTEAGGVVLIGDDEADQPEHRRGSFGKPAPGFEARILDPDTGAAVAVGKVGELCIRGPYLMQRYHKRSREECFDPDGWFH
TGDLVRADADGYFYFAGRLGAMIKTAGANVSAVEVEKAIAAVTGGATAYVVAIPDARRGQLVAAAVVWPDDRAALDPDAL
RERLKSELSAYKIPRRFASLRRADVPLLSSGKVDLRQLRKLFDA

Sequences:

>Translated_524_residues
MPGDFATVGATLRHQARRRGDHPLLICDAERISYAEADVRSAELARGLIALGAGKGTHVGLLHPNGARFVVAMLAAARIG
AVVVPFSTFVTARELREQLLDSDVEILLSARSFRSHDYARRLSEAVSETDFDPGRRLFCTAAPQLRRVLFAPQTVGAPGG
GIDPALLAAMEDDVQACDPLAIVYTSGSTSTPKGVVHTHGALLEHQRNLNGIRGLTADDRLFCNSPFFWIGGFAFGLLAT
LVAGSTLICSNATDAGATLDLLEAEKPTMTNGFSAGIAHLAEHPSFADRDLSSMRRGNLYPIMAVEARPADPELRHNMLG
MTEAGGVVLIGDDEADQPEHRRGSFGKPAPGFEARILDPDTGAAVAVGKVGELCIRGPYLMQRYHKRSREECFDPDGWFH
TGDLVRADADGYFYFAGRLGAMIKTAGANVSAVEVEKAIAAVTGGATAYVVAIPDARRGQLVAAAVVWPDDRAALDPDAL
RERLKSELSAYKIPRRFASLRRADVPLLSSGKVDLRQLRKLFDA
>Mature_523_residues
PGDFATVGATLRHQARRRGDHPLLICDAERISYAEADVRSAELARGLIALGAGKGTHVGLLHPNGARFVVAMLAAARIGA
VVVPFSTFVTARELREQLLDSDVEILLSARSFRSHDYARRLSEAVSETDFDPGRRLFCTAAPQLRRVLFAPQTVGAPGGG
IDPALLAAMEDDVQACDPLAIVYTSGSTSTPKGVVHTHGALLEHQRNLNGIRGLTADDRLFCNSPFFWIGGFAFGLLATL
VAGSTLICSNATDAGATLDLLEAEKPTMTNGFSAGIAHLAEHPSFADRDLSSMRRGNLYPIMAVEARPADPELRHNMLGM
TEAGGVVLIGDDEADQPEHRRGSFGKPAPGFEARILDPDTGAAVAVGKVGELCIRGPYLMQRYHKRSREECFDPDGWFHT
GDLVRADADGYFYFAGRLGAMIKTAGANVSAVEVEKAIAAVTGGATAYVVAIPDARRGQLVAAAVVWPDDRAALDPDALR
ERLKSELSAYKIPRRFASLRRADVPLLSSGKVDLRQLRKLFDA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=26.4492753623188, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI8923896, Length=548, Percent_Identity=25.5474452554745, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI187761345, Length=548, Percent_Identity=27.1897810218978, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761343, Length=548, Percent_Identity=27.1897810218978, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI253970489, Length=561, Percent_Identity=25.1336898395722, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI28416953, Length=538, Percent_Identity=26.7657992565056, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI38505220, Length=497, Percent_Identity=25.7545271629779, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI115511026, Length=501, Percent_Identity=23.5528942115768, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI42544132, Length=517, Percent_Identity=23.7911025145068, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=22.4137931034483, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=22.0703125, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=22.0703125, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI58082049, Length=513, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI27477105, Length=475, Percent_Identity=21.8947368421053, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788107, Length=546, Percent_Identity=25.2747252747253, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI221142682, Length=522, Percent_Identity=27.3946360153257, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=25.4, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790505, Length=565, Percent_Identity=24.7787610619469, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789201, Length=365, Percent_Identity=26.8493150684932, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1786529, Length=473, Percent_Identity=28.1183932346723, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786810, Length=351, Percent_Identity=26.4957264957265, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=26.6304347826087, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=26.6304347826087, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=26.6304347826087, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=27.007299270073, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538037, Length=519, Percent_Identity=25.626204238921, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=509, Percent_Identity=26.3261296660118, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=25.3759398496241, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=26.3052208835341, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=522, Percent_Identity=26.8199233716475, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=527, Percent_Identity=24.4781783681214, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=22.2648752399232, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=364, Percent_Identity=23.9010989010989, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=571, Percent_Identity=26.2697022767075, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=538, Percent_Identity=25.6505576208178, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319699, Length=489, Percent_Identity=24.1308793456033, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24581924, Length=550, Percent_Identity=25.6363636363636, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21355181, Length=481, Percent_Identity=25.987525987526, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648255, Length=379, Percent_Identity=26.3852242744063, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648253, Length=375, Percent_Identity=25.6, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356441, Length=328, Percent_Identity=26.5243902439024, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI18859661, Length=549, Percent_Identity=24.408014571949, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356947, Length=354, Percent_Identity=24.8587570621469, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648260, Length=476, Percent_Identity=24.7899159663866, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=302, Percent_Identity=26.158940397351, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21358303, Length=335, Percent_Identity=25.9701492537313, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648257, Length=321, Percent_Identity=26.1682242990654, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653035, Length=355, Percent_Identity=24.5070422535211, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 56204; Mature: 56072

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGDFATVGATLRHQARRRGDHPLLICDAERISYAEADVRSAELARGLIALGAGKGTHVG
CCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEE
LLHPNGARFVVAMLAAARIGAVVVPFSTFVTARELREQLLDSDVEILLSARSFRSHDYAR
EECCCCHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RLSEAVSETDFDPGRRLFCTAAPQLRRVLFAPQTVGAPGGGIDPALLAAMEDDVQACDPL
HHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
AIVYTSGSTSTPKGVVHTHGALLEHQRNLNGIRGLTADDRLFCNSPFFWIGGFAFGLLAT
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
LVAGSTLICSNATDAGATLDLLEAEKPTMTNGFSAGIAHLAEHPSFADRDLSSMRRGNLY
HHCCCEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCC
PIMAVEARPADPELRHNMLGMTEAGGVVLIGDDEADQPEHRRGSFGKPAPGFEARILDPD
EEEEEECCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCEEEECCC
TGAAVAVGKVGELCIRGPYLMQRYHKRSREECFDPDGWFHTGDLVRADADGYFYFAGRLG
CCCEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEECCCCEEEEHHHHH
AMIKTAGANVSAVEVEKAIAAVTGGATAYVVAIPDARRGQLVAAAVVWPDDRAALDPDAL
HHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHH
RERLKSELSAYKIPRRFASLRRADVPLLSSGKVDLRQLRKLFDA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PGDFATVGATLRHQARRRGDHPLLICDAERISYAEADVRSAELARGLIALGAGKGTHVG
CCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEE
LLHPNGARFVVAMLAAARIGAVVVPFSTFVTARELREQLLDSDVEILLSARSFRSHDYAR
EECCCCHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RLSEAVSETDFDPGRRLFCTAAPQLRRVLFAPQTVGAPGGGIDPALLAAMEDDVQACDPL
HHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
AIVYTSGSTSTPKGVVHTHGALLEHQRNLNGIRGLTADDRLFCNSPFFWIGGFAFGLLAT
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
LVAGSTLICSNATDAGATLDLLEAEKPTMTNGFSAGIAHLAEHPSFADRDLSSMRRGNLY
HHCCCEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCC
PIMAVEARPADPELRHNMLGMTEAGGVVLIGDDEADQPEHRRGSFGKPAPGFEARILDPD
EEEEEECCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCEEEECCC
TGAAVAVGKVGELCIRGPYLMQRYHKRSREECFDPDGWFHTGDLVRADADGYFYFAGRLG
CCCEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEECCCCEEEEHHHHH
AMIKTAGANVSAVEVEKAIAAVTGGATAYVVAIPDARRGQLVAAAVVWPDDRAALDPDAL
HHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHH
RERLKSELSAYKIPRRFASLRRADVPLLSSGKVDLRQLRKLFDA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]