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The map label for this gene is paaF [C]

Identifier: 41408496

GI number: 41408496

Start: 2693594

End: 2694406

Strand: Direct

Name: paaF [C]

Synonym: MAP2398

Alternate gene names: 41408496

Gene position: 2693594-2694406 (Clockwise)

Preceding gene: 41408495

Following gene: 41408497

Centisome position: 55.77

GC content: 66.91

Gene sequence:

>813_bases
ATGCCCGTTGATTCGTTCGACACGATCAAGTACGAGGTCGACGGGCACACCGCGACCATCACCCTGAACCGCCCGGACGC
CCTCAACGCGCTCAGCCCGCACATGATCACCGAACTGCGGGCCGCCTACCACGAGGCGGAGAACGACGACAGGGTGTGGC
TGCTCGTCGTCACCGGCACCGGCCGCGCGTTCTGCTCGGGCGCCGACGTCAAGGAGATCCCCGAAGACGGCAAGGTGATC
TACGAGCGGCCGTACCTGTCGACCTACGACCAATGGGAGGCCCCGCAGGAAGGGACGCCGCCGTTTCGCACCATGGCCAA
GCCGGTGCTGACCGCGGTGAACGGAATCTGTTGCGGCGCGGGCATGGACTGGGTCACGACGACCGACATCGTGATCGCGT
CCGAGCAGGCCACCTTCTTCGACCCGCACGTCAGCATCGGCCTGGTGGCCGGCCGTGAATTGGTCCGGGTGTCGCGGGTG
CTGCCCCGCTCGATCGCGCTGCGGATGGCGTTGATGGGCAAGCACGAGCGGATGAGCGCACAGCGCGCCTACGAGCTCGG
GCTGATCAGCGAGATCGTCGAGCACGACCGGCTGCTGGAGCGGGCGCACGAGATCGCCGACATCGTCAATTCTAATGCGC
CGCTGGCCGTTCGGGGCACCCGGCTGGCCATCCTCAAGGGCCTGAACGTGCCGCTGCACGAGGCGGAGATCCTCGCCGAG
ACCTTCCGCGAGCGGGTGTTGCGAACCGAGGACGCGGCCGAAGGACCGAGGGCCTTCGTGGAAAAGCGCCAACCGAATTG
GCAATGCCGATGA

Upstream 100 bases:

>100_bases
AGGCGCCGGGGCGTCGAGCGTGGGCGCCATGCACGAAAAGCGGCGCCCACCCGTATATACGGCCCACGCTCGGTGGCGCC
GGGATACGAAGGGATGACTC

Downstream 100 bases:

>100_bases
GCGCACAGTTCGACACCATCCTGCTCGAGGTGGACGCCACCGACCACGTCGCCACCATCACGCTCAATCGGCCCGAGCAG
CTCAACGCGTTCAACCGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPVDSFDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYHEAENDDRVWLLVVTGTGRAFCSGADVKEIPEDGKVI
YERPYLSTYDQWEAPQEGTPPFRTMAKPVLTAVNGICCGAGMDWVTTTDIVIASEQATFFDPHVSIGLVAGRELVRVSRV
LPRSIALRMALMGKHERMSAQRAYELGLISEIVEHDRLLERAHEIADIVNSNAPLAVRGTRLAILKGLNVPLHEAEILAE
TFRERVLRTEDAAEGPRAFVEKRQPNWQCR

Sequences:

>Translated_270_residues
MPVDSFDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYHEAENDDRVWLLVVTGTGRAFCSGADVKEIPEDGKVI
YERPYLSTYDQWEAPQEGTPPFRTMAKPVLTAVNGICCGAGMDWVTTTDIVIASEQATFFDPHVSIGLVAGRELVRVSRV
LPRSIALRMALMGKHERMSAQRAYELGLISEIVEHDRLLERAHEIADIVNSNAPLAVRGTRLAILKGLNVPLHEAEILAE
TFRERVLRTEDAAEGPRAFVEKRQPNWQCR
>Mature_269_residues
PVDSFDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYHEAENDDRVWLLVVTGTGRAFCSGADVKEIPEDGKVIY
ERPYLSTYDQWEAPQEGTPPFRTMAKPVLTAVNGICCGAGMDWVTTTDIVIASEQATFFDPHVSIGLVAGRELVRVSRVL
PRSIALRMALMGKHERMSAQRAYELGLISEIVEHDRLLERAHEIADIVNSNAPLAVRGTRLAILKGLNVPLHEAEILAET
FRERVLRTEDAAEGPRAFVEKRQPNWQCR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=269, Percent_Identity=33.8289962825279, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=30.6513409961686, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI70995211, Length=277, Percent_Identity=25.2707581227437, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594469, Length=206, Percent_Identity=27.1844660194175, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI37594471, Length=206, Percent_Identity=27.1844660194175, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI116235487, Length=187, Percent_Identity=31.0160427807487, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI221307494, Length=191, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=191, Percent_Identity=26.7015706806283, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI221136753, Length=191, Percent_Identity=26.7015706806283, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=31.3725490196078, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=30.0380228136882, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787660, Length=273, Percent_Identity=30.4029304029304, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788597, Length=270, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87082183, Length=275, Percent_Identity=25.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=229, Percent_Identity=28.82096069869, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=280, Percent_Identity=32.8571428571429, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=32.6771653543307, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=24.0740740740741, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=23.6641221374046, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=25.7028112449799, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=266, Percent_Identity=23.6842105263158, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=24.3027888446215, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=265, Percent_Identity=32.0754716981132, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653477, Length=265, Percent_Identity=32.0754716981132, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920382, Length=217, Percent_Identity=27.6497695852535, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=25.3968253968254, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653139, Length=281, Percent_Identity=27.7580071174377, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357171, Length=209, Percent_Identity=30.1435406698565, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24650670, Length=281, Percent_Identity=24.9110320284698, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30113; Mature: 29982

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVDSFDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYHEAENDDRVWLLVVTGT
CCCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECC
GRAFCSGADVKEIPEDGKVIYERPYLSTYDQWEAPQEGTPPFRTMAKPVLTAVNGICCGA
CCEEECCCCHHHCCCCCCEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCEEECC
GMDWVTTTDIVIASEQATFFDPHVSIGLVAGRELVRVSRVLPRSIALRMALMGKHERMSA
CCCCEEECEEEEECCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRAYELGLISEIVEHDRLLERAHEIADIVNSNAPLAVRGTRLAILKGLNVPLHEAEILAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCHHHHHHHHH
TFRERVLRTEDAAEGPRAFVEKRQPNWQCR
HHHHHHHCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
PVDSFDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYHEAENDDRVWLLVVTGT
CCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECC
GRAFCSGADVKEIPEDGKVIYERPYLSTYDQWEAPQEGTPPFRTMAKPVLTAVNGICCGA
CCEEECCCCHHHCCCCCCEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCEEECC
GMDWVTTTDIVIASEQATFFDPHVSIGLVAGRELVRVSRVLPRSIALRMALMGKHERMSA
CCCCEEECEEEEECCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRAYELGLISEIVEHDRLLERAHEIADIVNSNAPLAVRGTRLAILKGLNVPLHEAEILAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCHHHHHHHHH
TFRERVLRTEDAAEGPRAFVEKRQPNWQCR
HHHHHHHCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA