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The map label for this gene is fadD19_2

Identifier: 41408478

GI number: 41408478

Start: 2674275

End: 2675927

Strand: Direct

Name: fadD19_2

Synonym: MAP2380

Alternate gene names: 41408478

Gene position: 2674275-2675927 (Clockwise)

Preceding gene: 41408477

Following gene: 41408479

Centisome position: 55.37

GC content: 68.48

Gene sequence:

>1653_bases
ATGTCCGACACCACAACAGCATTCACGGTACCGGCGGTCGCGAAGGCCGTCGCGGCCGCGATCCCCGACCGCGAGCTGAT
CATCCAGGGCGACCGTCGCTACACCTACCGGCAGGTGATCGAACGGTCGAACCGGCTCGCCGCGTATCTGCACTCCCGAG
GTCTGGGATGCCACACCGAGCGCGAGGCGCTGGCCGGCCACGAGGTGGGCCAGGACCTGCTCGGCCTCTACGCGTACAAC
GGAAGCGAATTCGTCGAAGCGCTGCTGGGCGCCTTCGCTGCGCGCGTCGCCCCGTTCAACGTCAACTTCCGCTACGTCAA
AAGCGAACTGCACTACCTGCTCGCGGACTCCGAGGCGACCGCGCTGATCTACCACGCGGCGTTCGCGCCCCGGGTGGCCG
AGATCCTGCCCGAGCTGCCGCGGCTACGGGTGCTTATCCAGATCGCCGACGAGTCGGGCAACGAATTACTCGACGGCGCA
GTGGATTACGAGGACGCGCTGGCGTCGGTGTCCGCGGAGCCACCACCGGTGCGGCACTGTCCGGACGACCTGTACGTGCT
GTACACCGGCGGCACCACGGGAATGCCCAAGGGCGTGTTGTGGCGTCAGCACGACATCTTCATGACGTCCTTCGGGGGGC
GCAACCTGATGACCGGCGAGCCCTCGTCGTCGATCGACGAGATCGTGCAGCGCGCCGCGTCTGGCCCGGGGACCAAGCTG
ATGATCCTGCCGCCGCTGATCCACGGCGCGGCCCAGTGGAGCGTGATGACGGCGATCACGACCGGCCAGACGGTCGTCTT
CCCCACTGTCGTCGACCATTTGGACGCCGAGGACGTGGTGCGCACCATCGAGCGGGAAAAGGTCATGGTGGTGACGGTGG
TGGGTGACGCGATGGCCCGCCCGTTGGTCGCGGCCATCGAGAAGGGGATCGCCGACGTGTCGTCGCTGGCCGTGGTGGCC
AACGGCGGCGCGTTGCTGACCCCGTTCGTCAAGCAGCGCTTGATCGAAGTACTGCCGAACGCGGTGGTCGTCGACGGCGT
CGGGTCGTCGGAGACCGGGGCGCAGATGCACCACATGTCGACGCCCGGGGCGGTGGCGACCGGCACCTTCAACGCCGGCC
CGGACACCTTCGTGGCGGCCGAGGACCTGTCGGCGATCCTGCCGCCCGGGCACGAGGGGATGGGCTGGTTGGCCCAGCGC
GGCTATGTCCCGCTCGGGTACAAGGGCGATGCCGCCAAGACCGCCAAGACCTTTCCGGTCATCGACGGGGTGCGCTACGC
GGTGCCGGGCGACCGGGCACGCCACCACGCCGACGGCCATATCGAGCTGCTGGGCCGCGACTCCGTGTGCATCAATTCCG
GCGGCGAGAAGATTTTCGTCGAGGAGGTCGAGACGGCCATCGCGTCGCATCCCGCGGTGGCCGACGTGGTGGTGGCCGGA
CGGCCGAGTGAACGGTGGGGCCAGGAAGTCGTCGCCGTGGTCGCGCTGTCCGACGGCGCCGCCGTCGACGCCGGAGAATT
GATCGCCCACGCATCGAATTCGCTGGCGCGCTACAAGCTTCCCAAGGCGATCGTGTTCCGTCCGGTGATCGAGCGCAGCC
CGTCGGGCAAGGCCGATTACCGGTGGGCGCGCGAGCAGGCGGTGAACGGATGA

Upstream 100 bases:

>100_bases
CTTCATCTCCGCTAGCATGACGGCGGCCGACGGCACGCTGCTCAGTGAGGCCACCGGCCTGATGGTCCGCCTGCTACCCC
ACCAGCCGTGAGAGGCGAAA

Downstream 100 bases:

>100_bases
AACCCGCTGGGGCCGAGTGCTTTTAGGCTAGGAGCACACCGATGAAGTACCAAGGGCGGGTCGTGGTGGTCACAGGCGCC
GGCTCGGGCATCGGCCGGGC

Product: acyl-CoA synthetase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSDTTTAFTVPAVAKAVAAAIPDRELIIQGDRRYTYRQVIERSNRLAAYLHSRGLGCHTEREALAGHEVGQDLLGLYAYN
GSEFVEALLGAFAARVAPFNVNFRYVKSELHYLLADSEATALIYHAAFAPRVAEILPELPRLRVLIQIADESGNELLDGA
VDYEDALASVSAEPPPVRHCPDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRNLMTGEPSSSIDEIVQRAASGPGTKL
MILPPLIHGAAQWSVMTAITTGQTVVFPTVVDHLDAEDVVRTIEREKVMVVTVVGDAMARPLVAAIEKGIADVSSLAVVA
NGGALLTPFVKQRLIEVLPNAVVVDGVGSSETGAQMHHMSTPGAVATGTFNAGPDTFVAAEDLSAILPPGHEGMGWLAQR
GYVPLGYKGDAAKTAKTFPVIDGVRYAVPGDRARHHADGHIELLGRDSVCINSGGEKIFVEEVETAIASHPAVADVVVAG
RPSERWGQEVVAVVALSDGAAVDAGELIAHASNSLARYKLPKAIVFRPVIERSPSGKADYRWAREQAVNG

Sequences:

>Translated_550_residues
MSDTTTAFTVPAVAKAVAAAIPDRELIIQGDRRYTYRQVIERSNRLAAYLHSRGLGCHTEREALAGHEVGQDLLGLYAYN
GSEFVEALLGAFAARVAPFNVNFRYVKSELHYLLADSEATALIYHAAFAPRVAEILPELPRLRVLIQIADESGNELLDGA
VDYEDALASVSAEPPPVRHCPDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRNLMTGEPSSSIDEIVQRAASGPGTKL
MILPPLIHGAAQWSVMTAITTGQTVVFPTVVDHLDAEDVVRTIEREKVMVVTVVGDAMARPLVAAIEKGIADVSSLAVVA
NGGALLTPFVKQRLIEVLPNAVVVDGVGSSETGAQMHHMSTPGAVATGTFNAGPDTFVAAEDLSAILPPGHEGMGWLAQR
GYVPLGYKGDAAKTAKTFPVIDGVRYAVPGDRARHHADGHIELLGRDSVCINSGGEKIFVEEVETAIASHPAVADVVVAG
RPSERWGQEVVAVVALSDGAAVDAGELIAHASNSLARYKLPKAIVFRPVIERSPSGKADYRWAREQAVNG
>Mature_549_residues
SDTTTAFTVPAVAKAVAAAIPDRELIIQGDRRYTYRQVIERSNRLAAYLHSRGLGCHTEREALAGHEVGQDLLGLYAYNG
SEFVEALLGAFAARVAPFNVNFRYVKSELHYLLADSEATALIYHAAFAPRVAEILPELPRLRVLIQIADESGNELLDGAV
DYEDALASVSAEPPPVRHCPDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRNLMTGEPSSSIDEIVQRAASGPGTKLM
ILPPLIHGAAQWSVMTAITTGQTVVFPTVVDHLDAEDVVRTIEREKVMVVTVVGDAMARPLVAAIEKGIADVSSLAVVAN
GGALLTPFVKQRLIEVLPNAVVVDGVGSSETGAQMHHMSTPGAVATGTFNAGPDTFVAAEDLSAILPPGHEGMGWLAQRG
YVPLGYKGDAAKTAKTFPVIDGVRYAVPGDRARHHADGHIELLGRDSVCINSGGEKIFVEEVETAIASHPAVADVVVAGR
PSERWGQEVVAVVALSDGAAVDAGELIAHASNSLARYKLPKAIVFRPVIERSPSGKADYRWAREQAVNG

Specific function: Activates The Carboxylate Group Of 2,3-Dihydroxy- Benzoate (2,3-Dhb), Via ATP-Dependent Ppi Exchange Reactions, To The Acyladenylate. Then, Catalyzes The Acylation Of Holo-Entb With 2,3-Dhb Adenylate, Preparing That Molecule For Amide Bond Formation With

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=595, Percent_Identity=25.2100840336134, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI38505220, Length=454, Percent_Identity=25.3303964757709, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI187761345, Length=567, Percent_Identity=25.5731922398589, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI187761343, Length=567, Percent_Identity=25.5731922398589, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI115511026, Length=462, Percent_Identity=26.6233766233766, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI122937307, Length=465, Percent_Identity=25.1612903225806, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI157311624, Length=480, Percent_Identity=25, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=480, Percent_Identity=25, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI58082049, Length=480, Percent_Identity=24.5833333333333, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=25.8426966292135, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=25.0457038391225, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI145693145, Length=373, Percent_Identity=27.8820375335121, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=563, Percent_Identity=25.5772646536412, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=481, Percent_Identity=25.5717255717256, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=488, Percent_Identity=23.155737704918, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=505, Percent_Identity=24.950495049505, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24581924, Length=547, Percent_Identity=26.8738574040219, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI18859661, Length=528, Percent_Identity=24.4318181818182, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355181, Length=493, Percent_Identity=22.5152129817444, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45552801, Length=204, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550496, Length=204, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364798, Length=510, Percent_Identity=23.921568627451, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24583465, Length=510, Percent_Identity=23.921568627451, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24583463, Length=510, Percent_Identity=23.921568627451, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 58584; Mature: 58453

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTTAFTVPAVAKAVAAAIPDRELIIQGDRRYTYRQVIERSNRLAAYLHSRGLGCHTE
CCCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHEEHHHHCCCCCCCH
REALAGHEVGQDLLGLYAYNGSEFVEALLGAFAARVAPFNVNFRYVKSELHYLLADSEAT
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCE
ALIYHAAFAPRVAEILPELPRLRVLIQIADESGNELLDGAVDYEDALASVSAEPPPVRHC
EEEEEHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHCCCCCHHHHHHHCCCCCCCCCCC
PDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRNLMTGEPSSSIDEIVQRAASGPGTKL
CCCEEEEEECCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
MILPPLIHGAAQWSVMTAITTGQTVVFPTVVDHLDAEDVVRTIEREKVMVVTVVGDAMAR
EEECCHHCCCHHHEEEEEEECCCEEEEHHHHHHCCHHHHHHHHCCCCEEEEEEECCHHHH
PLVAAIEKGIADVSSLAVVANGGALLTPFVKQRLIEVLPNAVVVDGVGSSETGAQMHHMS
HHHHHHHHHHHHHHHEEEEECCCEEECHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECC
TPGAVATGTFNAGPDTFVAAEDLSAILPPGHEGMGWLAQRGYVPLGYKGDAAKTAKTFPV
CCCCEEECCCCCCCCCEEEHHHHHHHCCCCCCCCCHHHHCCEEECCCCCCCCCCCCCCCC
IDGVRYAVPGDRARHHADGHIELLGRDSVCINSGGEKIFVEEVETAIASHPAVADVVVAG
CCCEEEECCCCHHCCCCCCEEEEEECCCEEEECCCCEEEHHHHHHHHHCCCCCEEEEEEC
RPSERWGQEVVAVVALSDGAAVDAGELIAHASNSLARYKLPKAIVFRPVIERSPSGKADY
CCCHHHHHEEEEEEEECCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCCCH
RWAREQAVNG
HHHHHHCCCC
>Mature Secondary Structure 
SDTTTAFTVPAVAKAVAAAIPDRELIIQGDRRYTYRQVIERSNRLAAYLHSRGLGCHTE
CCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHEEHHHHCCCCCCCH
REALAGHEVGQDLLGLYAYNGSEFVEALLGAFAARVAPFNVNFRYVKSELHYLLADSEAT
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCE
ALIYHAAFAPRVAEILPELPRLRVLIQIADESGNELLDGAVDYEDALASVSAEPPPVRHC
EEEEEHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHCCCCCHHHHHHHCCCCCCCCCCC
PDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRNLMTGEPSSSIDEIVQRAASGPGTKL
CCCEEEEEECCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
MILPPLIHGAAQWSVMTAITTGQTVVFPTVVDHLDAEDVVRTIEREKVMVVTVVGDAMAR
EEECCHHCCCHHHEEEEEEECCCEEEEHHHHHHCCHHHHHHHHCCCCEEEEEEECCHHHH
PLVAAIEKGIADVSSLAVVANGGALLTPFVKQRLIEVLPNAVVVDGVGSSETGAQMHHMS
HHHHHHHHHHHHHHHEEEEECCCEEECHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECC
TPGAVATGTFNAGPDTFVAAEDLSAILPPGHEGMGWLAQRGYVPLGYKGDAAKTAKTFPV
CCCCEEECCCCCCCCCEEEHHHHHHHCCCCCCCCCHHHHCCEEECCCCCCCCCCCCCCCC
IDGVRYAVPGDRARHHADGHIELLGRDSVCINSGGEKIFVEEVETAIASHPAVADVVVAG
CCCEEEECCCCHHCCCCCCEEEEEECCCEEEECCCCEEEHHHHHHHHHCCCCCEEEEEEC
RPSERWGQEVVAVVALSDGAAVDAGELIAHASNSLARYKLPKAIVFRPVIERSPSGKADY
CCCHHHHHEEEEEEEECCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCCCH
RWAREQAVNG
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]