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The map label for this gene is leuC [H]

Identifier: 41408353

GI number: 41408353

Start: 2519467

End: 2520714

Strand: Direct

Name: leuC [H]

Synonym: MAP2255

Alternate gene names: 41408353

Gene position: 2519467-2520714 (Clockwise)

Preceding gene: 41408352

Following gene: 41408354

Centisome position: 52.17

GC content: 68.43

Gene sequence:

>1248_bases
ATGGGCATGACCGTCATCGAGAAGATCTTGGCCCGCAAGGCCGGGCTGGACTCCGTGTCATCGGGCGACACCGTCGTCGT
AGACGTCGACATGACCGTCCTGATCGACCTGCAATTCGCCACCATGTGGATATCCCCCACCCGGATTCACGACCCCGACA
GGCTCGCGGTGATCATGGACCACGCGGTGCCCGCGCCCACCATCAAGGACGCCGACGGCGGCCGGCAGGCCCGCAGGTTC
GTGGCCGACTTCGGCATCGAGCGCTTCTACGACGTCGGCCGGCACGGCATCTGCCACCAGGTGATCGCCGAGAACGGGCT
GGCCCGTCCGGGAGAAGTGCTGGCCTGCACCGACTCTCATACCTGCGCGGCCGGCGCCTACAACAACGCCGCCCGTGGTC
TGGGGCCCGCCGAAATCTACTCGATCATGTGCACCGGCACCACCTGGTTCCAGGTCGCGCCGACCATCCGCTACGAATTC
CACGGCGCCAAGCCGGAGGTGGTGAGCGGAAAGGACATCTTCCTGCACATCGCCGACGAGTACGGTGACGCCCCCAACCG
GAACCTCGAGTTCGGCGGGCCGGGCCTGGCCAGCATCCCGATGCACGACCGGCGGACCATCGCCACCCAGGGCGCGGAGG
TGTCCGCCGATTTCAGCACCTTCGAGCCCGACGACGTGCTGACGTCGTTCCTGGCCGAACGCGGTGTCGTCGAATACGAG
GCCGTCACACCGGATTCCGACGCGGCATACCACGACGTCCGACGCGTCGACCTGGCGACGCTGGAACCCTACGTGGCACG
GCCGGGCACCGTGAGCCGCAACGGTTCACCGGTGTCGCGGTTGGGCAGGCAGAAGATCGACCAGGCCTTCATCGGCTCCT
GCGCCAACGGGCAGCTGGAGGACCTGCGCATCGCCGCCGAGGTGCTGCGCGGCAAGACCGTCGCGCCGGGCGTGCGGCTG
CTGATCACCCCCGCCTCGCAGGCCGTGTATCGAGAGGCCTTGCGGCGCGGCTACCTTCAGGACCTCGCCGACGCCGGCGC
CGTCGTCACCAATTCCACCTGCGGGGCCTGCTTCGGCTACCACATGGGCCTGCTGGGGGCCGGCGAGGTGTGCATCACCG
CGAGCACCCGCAACTTCACCGGCCGGATGGGCAGCACCGAAGCCGAAATCTTCATGGCCTCGCCGGCCACCGTCGCGGCG
TCCGCGGTCGCCGGCTACATCACCGACCCGAGGAGTGTGACCGCATGA

Upstream 100 bases:

>100_bases
CCGCCCATCTGCCGGCGTCTCCGGGCGATTTCTTCGACCTGGTGGGCATGGCGCACTGGCGGGCCCTCGACGAGAAGTAC
GCACACACCGGGGGCCGCAC

Downstream 100 bases:

>100_bases
CGCTGCGCTCGCTAATGTTCCGCGGCAAGGTCTGGGTGTTCGGCGACAACCTGAACACCGACGCCATGTACCCGGCGTTC
GCGATGAAGATGCAGCCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGMTVIEKILARKAGLDSVSSGDTVVVDVDMTVLIDLQFATMWISPTRIHDPDRLAVIMDHAVPAPTIKDADGGRQARRF
VADFGIERFYDVGRHGICHQVIAENGLARPGEVLACTDSHTCAAGAYNNAARGLGPAEIYSIMCTGTTWFQVAPTIRYEF
HGAKPEVVSGKDIFLHIADEYGDAPNRNLEFGGPGLASIPMHDRRTIATQGAEVSADFSTFEPDDVLTSFLAERGVVEYE
AVTPDSDAAYHDVRRVDLATLEPYVARPGTVSRNGSPVSRLGRQKIDQAFIGSCANGQLEDLRIAAEVLRGKTVAPGVRL
LITPASQAVYREALRRGYLQDLADAGAVVTNSTCGACFGYHMGLLGAGEVCITASTRNFTGRMGSTEAEIFMASPATVAA
SAVAGYITDPRSVTA

Sequences:

>Translated_415_residues
MGMTVIEKILARKAGLDSVSSGDTVVVDVDMTVLIDLQFATMWISPTRIHDPDRLAVIMDHAVPAPTIKDADGGRQARRF
VADFGIERFYDVGRHGICHQVIAENGLARPGEVLACTDSHTCAAGAYNNAARGLGPAEIYSIMCTGTTWFQVAPTIRYEF
HGAKPEVVSGKDIFLHIADEYGDAPNRNLEFGGPGLASIPMHDRRTIATQGAEVSADFSTFEPDDVLTSFLAERGVVEYE
AVTPDSDAAYHDVRRVDLATLEPYVARPGTVSRNGSPVSRLGRQKIDQAFIGSCANGQLEDLRIAAEVLRGKTVAPGVRL
LITPASQAVYREALRRGYLQDLADAGAVVTNSTCGACFGYHMGLLGAGEVCITASTRNFTGRMGSTEAEIFMASPATVAA
SAVAGYITDPRSVTA
>Mature_414_residues
GMTVIEKILARKAGLDSVSSGDTVVVDVDMTVLIDLQFATMWISPTRIHDPDRLAVIMDHAVPAPTIKDADGGRQARRFV
ADFGIERFYDVGRHGICHQVIAENGLARPGEVLACTDSHTCAAGAYNNAARGLGPAEIYSIMCTGTTWFQVAPTIRYEFH
GAKPEVVSGKDIFLHIADEYGDAPNRNLEFGGPGLASIPMHDRRTIATQGAEVSADFSTFEPDDVLTSFLAERGVVEYEA
VTPDSDAAYHDVRRVDLATLEPYVARPGTVSRNGSPVSRLGRQKIDQAFIGSCANGQLEDLRIAAEVLRGKTVAPGVRLL
ITPASQAVYREALRRGYLQDLADAGAVVTNSTCGACFGYHMGLLGAGEVCITASTRNFTGRMGSTEAEIFMASPATVAAS
AVAGYITDPRSVTA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=366, Percent_Identity=30.0546448087432, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786259, Length=417, Percent_Identity=29.4964028776978, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081781, Length=355, Percent_Identity=26.4788732394366, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25149337, Length=369, Percent_Identity=29.5392953929539, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32564738, Length=369, Percent_Identity=29.5392953929539, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25149342, Length=270, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320440, Length=433, Percent_Identity=32.7944572748268, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321429, Length=411, Percent_Identity=27.2506082725061, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=345, Percent_Identity=29.8550724637681, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=356, Percent_Identity=27.247191011236, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571643, Length=346, Percent_Identity=31.5028901734104, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161076999, Length=345, Percent_Identity=28.1159420289855, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281365315, Length=345, Percent_Identity=28.1159420289855, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17864292, Length=345, Percent_Identity=28.1159420289855, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44273; Mature: 44142

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTVIEKILARKAGLDSVSSGDTVVVDVDMTVLIDLQFATMWISPTRIHDPDRLAVIMD
CCHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEEEEEEEEEEEECCCCCCCCCCEEEEEE
HAVPAPTIKDADGGRQARRFVADFGIERFYDVGRHGICHQVIAENGLARPGEVLACTDSH
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEECCCC
TCAAGAYNNAARGLGPAEIYSIMCTGTTWFQVAPTIRYEFHGAKPEVVSGKDIFLHIADE
CEECCCCCCHHCCCCHHHEEEEEECCCEEEEECCEEEEEECCCCCCEECCCEEEEEEEHH
YGDAPNRNLEFGGPGLASIPMHDRRTIATQGAEVSADFSTFEPDDVLTSFLAERGVVEYE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHCCCEEEE
AVTPDSDAAYHDVRRVDLATLEPYVARPGTVSRNGSPVSRLGRQKIDQAFIGSCANGQLE
EECCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
DLRIAAEVLRGKTVAPGVRLLITPASQAVYREALRRGYLQDLADAGAVVTNSTCGACFGY
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
HMGLLGAGEVCITASTRNFTGRMGSTEAEIFMASPATVAASAVAGYITDPRSVTA
HHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GMTVIEKILARKAGLDSVSSGDTVVVDVDMTVLIDLQFATMWISPTRIHDPDRLAVIMD
CHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEEEEEEEEEEEECCCCCCCCCCEEEEEE
HAVPAPTIKDADGGRQARRFVADFGIERFYDVGRHGICHQVIAENGLARPGEVLACTDSH
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEECCCC
TCAAGAYNNAARGLGPAEIYSIMCTGTTWFQVAPTIRYEFHGAKPEVVSGKDIFLHIADE
CEECCCCCCHHCCCCHHHEEEEEECCCEEEEECCEEEEEECCCCCCEECCCEEEEEEEHH
YGDAPNRNLEFGGPGLASIPMHDRRTIATQGAEVSADFSTFEPDDVLTSFLAERGVVEYE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHCCCEEEE
AVTPDSDAAYHDVRRVDLATLEPYVARPGTVSRNGSPVSRLGRQKIDQAFIGSCANGQLE
EECCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
DLRIAAEVLRGKTVAPGVRLLITPASQAVYREALRRGYLQDLADAGAVVTNSTCGACFGY
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
HMGLLGAGEVCITASTRNFTGRMGSTEAEIFMASPATVAASAVAGYITDPRSVTA
HHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA