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The map label for this gene is asnB_1

Identifier: 41407519

GI number: 41407519

Start: 1551687

End: 1553492

Strand: Direct

Name: asnB_1

Synonym: MAP1421

Alternate gene names: 41407519

Gene position: 1551687-1553492 (Clockwise)

Preceding gene: 41407518

Following gene: 41407520

Centisome position: 32.13

GC content: 70.27

Gene sequence:

>1806_bases
GTGTGCGGAGTCACCGGGGAGGTACGACTCGACGGGCGGGCGCCCGATGTAGCCGCGGTCTCGGCGATGGCCGCCGTGCT
GACACCACGCGGCCCCGACGCCGGCGGCGCCTGGTCGCAGGGCCGCGTCGCGCTCGGGCATCGTCGCCTCAAGATCATCG
ACCTGACCGAAGCCGGCGCCCAGCCGATGGTCGACTCCGAGCTGGGCCTATCCATCGCCTGGAACGGCTGCATCTACAAC
TACAAGGAACTGCGCAGCGAGCTGTCCGGCTACGGCTACCGGTTCTTCTCGCACAGCGACACCGAGGTCCTGATCAAGGC
CTACCACCGGTGGGGCGACGACTTCGTCAGCCATCTGTTCGGCATGTTCGCCTTCGCCATCGTCGAACGCGACAGCGGTC
GGGTGCTGCTCGGCCGCGACCGCCTCGGCATCAAACCCCTCTACCTGACCGAGGACAGTCACCGGGTCCGGTTCGCCTCG
ACGCTGCCCGCGCTGCTGGCCGGCGGCGGCGTGGACACCCGCATCGACCCGGTCGCCCTGCACCACTACATGAGCTTCCA
CTCGGTGGTGCCCGCCCCGGCCACCATCCTGCGCGGGGTCCGCAAGGTGCCGCCCGCCTCGCTGGTCGCCATCGAGCCCG
ACGGCAAACGCACCGTCACCACCTACTGGGCGCCCGATTTCACCCGGCACGCCGACCGCGCCGACTGGTCCGAACGCGAC
TGGGAGGACGCCGTGCTGTCCACCCTGCGGCTGGCCGTCAAGCGCCGGCTGGTGGCCGACGTCCCGGTGGGCTGCCTGCT
GTCCGGTGGTGTCGACTCCAGCCTGATCGTGGGTCTGCTCGCCGAAGCCGGCCAGCACGGGCTGTCCACCTTCTCCATCG
GCTTCGAGTCGGCGGGCGGGGTGAAGGGCGACGAGTTCCAGTACTCCGACATCGTGGCCCAGCGCTTCGAGACCAACCAC
CACCAGATCCGCATCGACTCCGACCGGATGCTGCCCGCGCTGGACGGCGCCATCGGCGCGATGAGCGAACCGATGGTCAG
CCACGACTGCGTCGCCTTCTACCTGCTCAGCCAGGAAGTGGCCCGGCACGTCAAGGTGGTGCAGTCCGGGCAGGGCGCCG
ACGAGGTGTTCGCCGGCTACCACTGGTACCCGCCGATGGGCGAGCCGGCCGCCGCCACCCTGGACGGGGCCGTCGCCCAG
TACCGGCGGGCCTTCTTCGACCGGGACACCGCCGGCGTCGCGCAGCTGGTCGGACCCGGCATCCTGGCCCCGGGCGACCC
CAGCCTGCGGTTCGTCACCGAACACTTCGCCCAGGCCGGCGCCGAGACCGGCATCGACCGCGCGTTGCGGCTCGACACCA
CGGTGATGCTGATCGACGACCCGGTGAAGCGGGTCGACAACATGACGATGGCCTGGGGGCTGGAGGGCCGGGTGCCCTTC
CTCGACCACGAGCTGGTCGAGCTGGCCGCCACCTGCCCGCCGGAGTACAAGATCGCCCACGAAGGCAAGGGCGTGCTCAA
ACAGGCTGCGCGACGGGTCATTCCGTCCGAGGTGATCGACCGGCCCAAGGGCTACTTCCCGGTCCCGGCGCTGACCCACC
TGGAAGGCCCCTACCTGGACATGGACCGCGACGCGCTGTACGCGCCGACCGCCAAGGAACGCGGTCTGTTCAACACCGAC
GCGGTCGACGCGCTGCTGGCCAACCCCAACGGCAAGCTGACCCCGCTGCGCGGCAACGAGCTGTGGCAGATCGCCCTGCT
CGAGCTGTGGCTGCAGCGGCACGGTGTGACGGGCCCGGTTGCATGA

Upstream 100 bases:

>100_bases
GGTGATCACCACGCACGGATCGCGGCCGTAGCTGGGCCGTTGCAGCGCTTTTGGCGGAGGTCACGCGCGCTGGGAATCGG
GCAACGGTAGGGTCGAAGAG

Downstream 100 bases:

>100_bases
CCATGACCGATCCGGCAGAGGATCACCCTTCCGAGGCGATCACCCTCGGGCTGCATGACGCGTCGCCGCCCGAGATGGTG
GACGCGATGGCCAAGGACGT

Product: AsnB_1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MCGVTGEVRLDGRAPDVAAVSAMAAVLTPRGPDAGGAWSQGRVALGHRRLKIIDLTEAGAQPMVDSELGLSIAWNGCIYN
YKELRSELSGYGYRFFSHSDTEVLIKAYHRWGDDFVSHLFGMFAFAIVERDSGRVLLGRDRLGIKPLYLTEDSHRVRFAS
TLPALLAGGGVDTRIDPVALHHYMSFHSVVPAPATILRGVRKVPPASLVAIEPDGKRTVTTYWAPDFTRHADRADWSERD
WEDAVLSTLRLAVKRRLVADVPVGCLLSGGVDSSLIVGLLAEAGQHGLSTFSIGFESAGGVKGDEFQYSDIVAQRFETNH
HQIRIDSDRMLPALDGAIGAMSEPMVSHDCVAFYLLSQEVARHVKVVQSGQGADEVFAGYHWYPPMGEPAAATLDGAVAQ
YRRAFFDRDTAGVAQLVGPGILAPGDPSLRFVTEHFAQAGAETGIDRALRLDTTVMLIDDPVKRVDNMTMAWGLEGRVPF
LDHELVELAATCPPEYKIAHEGKGVLKQAARRVIPSEVIDRPKGYFPVPALTHLEGPYLDMDRDALYAPTAKERGLFNTD
AVDALLANPNGKLTPLRGNELWQIALLELWLQRHGVTGPVA

Sequences:

>Translated_601_residues
MCGVTGEVRLDGRAPDVAAVSAMAAVLTPRGPDAGGAWSQGRVALGHRRLKIIDLTEAGAQPMVDSELGLSIAWNGCIYN
YKELRSELSGYGYRFFSHSDTEVLIKAYHRWGDDFVSHLFGMFAFAIVERDSGRVLLGRDRLGIKPLYLTEDSHRVRFAS
TLPALLAGGGVDTRIDPVALHHYMSFHSVVPAPATILRGVRKVPPASLVAIEPDGKRTVTTYWAPDFTRHADRADWSERD
WEDAVLSTLRLAVKRRLVADVPVGCLLSGGVDSSLIVGLLAEAGQHGLSTFSIGFESAGGVKGDEFQYSDIVAQRFETNH
HQIRIDSDRMLPALDGAIGAMSEPMVSHDCVAFYLLSQEVARHVKVVQSGQGADEVFAGYHWYPPMGEPAAATLDGAVAQ
YRRAFFDRDTAGVAQLVGPGILAPGDPSLRFVTEHFAQAGAETGIDRALRLDTTVMLIDDPVKRVDNMTMAWGLEGRVPF
LDHELVELAATCPPEYKIAHEGKGVLKQAARRVIPSEVIDRPKGYFPVPALTHLEGPYLDMDRDALYAPTAKERGLFNTD
AVDALLANPNGKLTPLRGNELWQIALLELWLQRHGVTGPVA
>Mature_601_residues
MCGVTGEVRLDGRAPDVAAVSAMAAVLTPRGPDAGGAWSQGRVALGHRRLKIIDLTEAGAQPMVDSELGLSIAWNGCIYN
YKELRSELSGYGYRFFSHSDTEVLIKAYHRWGDDFVSHLFGMFAFAIVERDSGRVLLGRDRLGIKPLYLTEDSHRVRFAS
TLPALLAGGGVDTRIDPVALHHYMSFHSVVPAPATILRGVRKVPPASLVAIEPDGKRTVTTYWAPDFTRHADRADWSERD
WEDAVLSTLRLAVKRRLVADVPVGCLLSGGVDSSLIVGLLAEAGQHGLSTFSIGFESAGGVKGDEFQYSDIVAQRFETNH
HQIRIDSDRMLPALDGAIGAMSEPMVSHDCVAFYLLSQEVARHVKVVQSGQGADEVFAGYHWYPPMGEPAAATLDGAVAQ
YRRAFFDRDTAGVAQLVGPGILAPGDPSLRFVTEHFAQAGAETGIDRALRLDTTVMLIDDPVKRVDNMTMAWGLEGRVPF
LDHELVELAATCPPEYKIAHEGKGVLKQAARRVIPSEVIDRPKGYFPVPALTHLEGPYLDMDRDALYAPTAKERGLFNTD
AVDALLANPNGKLTPLRGNELWQIALLELWLQRHGVTGPVA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=560, Percent_Identity=26.0714285714286, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI168229252, Length=560, Percent_Identity=26.0714285714286, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI168229250, Length=560, Percent_Identity=26.0714285714286, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI296010848, Length=530, Percent_Identity=26.2264150943396, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI296010852, Length=465, Percent_Identity=25.5913978494624, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI296010850, Length=465, Percent_Identity=25.5913978494624, Blast_Score=110, Evalue=5e-24,
Organism=Escherichia coli, GI1786889, Length=402, Percent_Identity=30.0995024875622, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=556, Percent_Identity=26.978417266187, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=556, Percent_Identity=26.978417266187, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=365, Percent_Identity=28.2191780821918, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325403, Length=577, Percent_Identity=27.0363951473137, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321563, Length=633, Percent_Identity=25.5924170616114, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45553209, Length=366, Percent_Identity=30.6010928961749, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65469; Mature: 65469

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVTGEVRLDGRAPDVAAVSAMAAVLTPRGPDAGGAWSQGRVALGHRRLKIIDLTEAGA
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCC
QPMVDSELGLSIAWNGCIYNYKELRSELSGYGYRFFSHSDTEVLIKAYHRWGDDFVSHLF
CCCCCCCCCEEEEECCEECCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GMFAFAIVERDSGRVLLGRDRLGIKPLYLTEDSHRVRFASTLPALLAGGGVDTRIDPVAL
HHHHHHHEECCCCCEEEECCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCHHHH
HHYMSFHSVVPAPATILRGVRKVPPASLVAIEPDGKRTVTTYWAPDFTRHADRADWSERD
HHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCHHHCCCCCCCCCCC
WEDAVLSTLRLAVKRRLVADVPVGCLLSGGVDSSLIVGLLAEAGQHGLSTFSIGFESAGG
HHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
VKGDEFQYSDIVAQRFETNHHQIRIDSDRMLPALDGAIGAMSEPMVSHDCVAFYLLSQEV
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ARHVKVVQSGQGADEVFAGYHWYPPMGEPAAATLDGAVAQYRRAFFDRDTAGVAQLVGPG
HHHHHHHHCCCCHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
ILAPGDPSLRFVTEHFAQAGAETGIDRALRLDTTVMLIDDPVKRVDNMTMAWGLEGRVPF
CCCCCCCCHHHHHHHHHHHCHHHCCHHEEEECEEEEEECCHHHHHCCCEEEECCCCCCCC
LDHELVELAATCPPEYKIAHEGKGVLKQAARRVIPSEVIDRPKGYFPVPALTHLEGPYLD
HHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHCCCCCCC
MDRDALYAPTAKERGLFNTDAVDALLANPNGKLTPLRGNELWQIALLELWLQRHGVTGPV
CCCCCEECCCHHHCCCCCCCHHHHHEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
A
C
>Mature Secondary Structure
MCGVTGEVRLDGRAPDVAAVSAMAAVLTPRGPDAGGAWSQGRVALGHRRLKIIDLTEAGA
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCC
QPMVDSELGLSIAWNGCIYNYKELRSELSGYGYRFFSHSDTEVLIKAYHRWGDDFVSHLF
CCCCCCCCCEEEEECCEECCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GMFAFAIVERDSGRVLLGRDRLGIKPLYLTEDSHRVRFASTLPALLAGGGVDTRIDPVAL
HHHHHHHEECCCCCEEEECCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCHHHH
HHYMSFHSVVPAPATILRGVRKVPPASLVAIEPDGKRTVTTYWAPDFTRHADRADWSERD
HHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCHHHCCCCCCCCCCC
WEDAVLSTLRLAVKRRLVADVPVGCLLSGGVDSSLIVGLLAEAGQHGLSTFSIGFESAGG
HHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
VKGDEFQYSDIVAQRFETNHHQIRIDSDRMLPALDGAIGAMSEPMVSHDCVAFYLLSQEV
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ARHVKVVQSGQGADEVFAGYHWYPPMGEPAAATLDGAVAQYRRAFFDRDTAGVAQLVGPG
HHHHHHHHCCCCHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
ILAPGDPSLRFVTEHFAQAGAETGIDRALRLDTTVMLIDDPVKRVDNMTMAWGLEGRVPF
CCCCCCCCHHHHHHHHHHHCHHHCCHHEEEECEEEEEECCHHHHHCCCEEEECCCCCCCC
LDHELVELAATCPPEYKIAHEGKGVLKQAARRVIPSEVIDRPKGYFPVPALTHLEGPYLD
HHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHCCCCCCC
MDRDALYAPTAKERGLFNTDAVDALLANPNGKLTPLRGNELWQIALLELWLQRHGVTGPV
CCCCCEECCCHHHCCCCCCCHHHHHEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]