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The map label for this gene is gshAB [H]

Identifier: 41407520

GI number: 41407520

Start: 1553495

End: 1555282

Strand: Direct

Name: gshAB [H]

Synonym: MAP1422

Alternate gene names: 41407520

Gene position: 1553495-1555282 (Clockwise)

Preceding gene: 41407519

Following gene: 41407521

Centisome position: 32.16

GC content: 70.97

Gene sequence:

>1788_bases
ATGACCGATCCGGCAGAGGATCACCCTTCCGAGGCGATCACCCTCGGGCTGCATGACGCGTCGCCGCCCGAGATGGTGGA
CGCGATGGCCAAGGACGTCGAGCTCGAATTGGGTTGGGGCCGACTGATTTTCGGGCAAACCTTCGCCAATTCCCAGGAGC
TGGTCGAGGCGCTGCGGCGGGAGGGGCCCGGCCGGCGCGACATCTGCATCTACGCCCGCGAATCCCATGTCGTGGTCGCC
GAGGCGCCCACCGAGCTCTTCATCGACCCCAGCCACACCTACCGGCTGCGGTTCACTGGTAAGGAGAAGAGCCTGGCGCC
CACACCGCTGGGCGTCACGGTCCGCACGCTCAACGACCCGATCGACGCCGACGCGATGAACCGGGTGTACGTGCGCTGCG
GCATGGTCCCGGCCGGGGTGGACGTGATCTGGGAGAACCATCAGCACGTGCCCGCGGTGAAATATCTCCTCGCGGTGCGC
GACGAGGACGGCGCCGTGGTCGGCACCGTCACCGGCGTCGACCACGAGTTGCTGTTCAACGACCCCGAGCACGGGTCGAG
CCTGTGGACGCTGGCCGTCGACCCGGCCGCCGGGCTGCCCGGGGTGGGCGGCGCCCTGACCCGGGCGCTGGCCGAGCACT
TCCGCAGTGCCGGCTGCGCCTACATGGATCTGTCGGTGGCGCACGACAATGCCGCCGCCATCAGCCTGTACGAGAAGCTG
GGCTTCCGCCGCGTGCCGGTGCTGGCCATCAAGCGCAAGAACGCGATCAACGAGCCGCTGTTCAGCCCGCCGCCGGAGAC
GGTCGACGACCTCAATCCCTACGCCCGGATCATCGCCGACGAGGCGCTGCGCCGCGGGATCTGGGTCGAGGTGCTCGACG
CCGAGACCGGCGAGATGCGGCTGACGCACGGCGGCCGCAGCGTCATCACCCGCGAATCGCTGTCCGAGTACACCTCGGCG
GTGGCCATGTGCCGGTGCGACGACAAGCGGTTGACCCGCCGCCTGGTCTCTGAGGCGGGCATCACCGTGCCGCGCGCCCG
GCTGGCCACCTTCGACGAGGGCGACTTCGCGTTCCTCAAGGAGGTCGGCGAGGTCGTCGTCAAACCCACCCGCGGCGAGC
AGGGCAAGGGCATCACGGTCGGGGTCACCGCCGAGAACGGGCCCGACGACCTGTCCGCCGCCCTGGCCCGGGCCCGGGCG
CAATACCCGGACGTGCTGATCGAGCAGCGGGTGCCCGGCGACGACCTTCGGCTGGTGGTGATCGACGGCCGTGTCGTCGC
CGCCGCGCTGCGGCTGCCGCCGGAGGTCATCGGCACCGGCGAGCACAGCGTGCGGGACCTGATCGCCGCCGAGAGCCGGC
GCCGCTCGGCGGCCACCGGCGGCGAATCGCGCATCCCGCTCGACGAACTCACCGAGGCCACCGTCGCCGAAGCCGGCTGG
AAGCTCGACGACGTGCTGCCCCAGGGCACCCGGCTGCGGGTGCGCCGCACCGCCAACCTGCATCAGGGCGGCACCATCCA
CGACGTCACCGCGCAGGTCAACAGCGAACTGTGCCGGGTCGCGGTGACCGCGGCCGAAGCGATCGGCATCCCGGTGACCG
GCATCGACCTGCTCGTGCCGGACGTGACCGGCGCCGACTACGCATTCATCGAAGCCAACGAGCGGCCCGGGCTGGCCAAC
CACGAACCGCAGCCCACCGCCGCGGCCTTCATCGACTTCCTGTTCCCCGGTCACCCCGGCCAGCCGCAGGCCTGGACCCC
CGAGGAATCCCGCTTTGCCCGGGGCTGA

Upstream 100 bases:

>100_bases
ACGGCAAGCTGACCCCGCTGCGCGGCAACGAGCTGTGGCAGATCGCCCTGCTCGAGCTGTGGCTGCAGCGGCACGGTGTG
ACGGGCCCGGTTGCATGACC

Downstream 100 bases:

>100_bases
CGCATGAGCGCCGGCGCGCAGACCAACACCATCACCACACACGTGCCGGCGCGGCTGGACCGGCTGCCGTGGTCGCGGTT
CCACTGGCGGGTGGTGATCG

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MTDPAEDHPSEAITLGLHDASPPEMVDAMAKDVELELGWGRLIFGQTFANSQELVEALRREGPGRRDICIYARESHVVVA
EAPTELFIDPSHTYRLRFTGKEKSLAPTPLGVTVRTLNDPIDADAMNRVYVRCGMVPAGVDVIWENHQHVPAVKYLLAVR
DEDGAVVGTVTGVDHELLFNDPEHGSSLWTLAVDPAAGLPGVGGALTRALAEHFRSAGCAYMDLSVAHDNAAAISLYEKL
GFRRVPVLAIKRKNAINEPLFSPPPETVDDLNPYARIIADEALRRGIWVEVLDAETGEMRLTHGGRSVITRESLSEYTSA
VAMCRCDDKRLTRRLVSEAGITVPRARLATFDEGDFAFLKEVGEVVVKPTRGEQGKGITVGVTAENGPDDLSAALARARA
QYPDVLIEQRVPGDDLRLVVIDGRVVAAALRLPPEVIGTGEHSVRDLIAAESRRRSAATGGESRIPLDELTEATVAEAGW
KLDDVLPQGTRLRVRRTANLHQGGTIHDVTAQVNSELCRVAVTAAEAIGIPVTGIDLLVPDVTGADYAFIEANERPGLAN
HEPQPTAAAFIDFLFPGHPGQPQAWTPEESRFARG

Sequences:

>Translated_595_residues
MTDPAEDHPSEAITLGLHDASPPEMVDAMAKDVELELGWGRLIFGQTFANSQELVEALRREGPGRRDICIYARESHVVVA
EAPTELFIDPSHTYRLRFTGKEKSLAPTPLGVTVRTLNDPIDADAMNRVYVRCGMVPAGVDVIWENHQHVPAVKYLLAVR
DEDGAVVGTVTGVDHELLFNDPEHGSSLWTLAVDPAAGLPGVGGALTRALAEHFRSAGCAYMDLSVAHDNAAAISLYEKL
GFRRVPVLAIKRKNAINEPLFSPPPETVDDLNPYARIIADEALRRGIWVEVLDAETGEMRLTHGGRSVITRESLSEYTSA
VAMCRCDDKRLTRRLVSEAGITVPRARLATFDEGDFAFLKEVGEVVVKPTRGEQGKGITVGVTAENGPDDLSAALARARA
QYPDVLIEQRVPGDDLRLVVIDGRVVAAALRLPPEVIGTGEHSVRDLIAAESRRRSAATGGESRIPLDELTEATVAEAGW
KLDDVLPQGTRLRVRRTANLHQGGTIHDVTAQVNSELCRVAVTAAEAIGIPVTGIDLLVPDVTGADYAFIEANERPGLAN
HEPQPTAAAFIDFLFPGHPGQPQAWTPEESRFARG
>Mature_594_residues
TDPAEDHPSEAITLGLHDASPPEMVDAMAKDVELELGWGRLIFGQTFANSQELVEALRREGPGRRDICIYARESHVVVAE
APTELFIDPSHTYRLRFTGKEKSLAPTPLGVTVRTLNDPIDADAMNRVYVRCGMVPAGVDVIWENHQHVPAVKYLLAVRD
EDGAVVGTVTGVDHELLFNDPEHGSSLWTLAVDPAAGLPGVGGALTRALAEHFRSAGCAYMDLSVAHDNAAAISLYEKLG
FRRVPVLAIKRKNAINEPLFSPPPETVDDLNPYARIIADEALRRGIWVEVLDAETGEMRLTHGGRSVITRESLSEYTSAV
AMCRCDDKRLTRRLVSEAGITVPRARLATFDEGDFAFLKEVGEVVVKPTRGEQGKGITVGVTAENGPDDLSAALARARAQ
YPDVLIEQRVPGDDLRLVVIDGRVVAAALRLPPEVIGTGEHSVRDLIAAESRRRSAATGGESRIPLDELTEATVAEAGWK
LDDVLPQGTRLRVRRTANLHQGGTIHDVTAQVNSELCRVAVTAAEAIGIPVTGIDLLVPDVTGADYAFIEANERPGLANH
EPQPTAAAFIDFLFPGHPGQPQAWTPEESRFARG

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003806
- InterPro:   IPR013815
- InterPro:   IPR007370
- InterPro:   IPR006335 [H]

Pfam domain/function: PF02655 ATP-grasp_3; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 64347; Mature: 64216

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPAEDHPSEAITLGLHDASPPEMVDAMAKDVELELGWGRLIFGQTFANSQELVEALRR
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCEEEEECCCEEEECHHHCCHHHHHHHHHH
EGPGRRDICIYARESHVVVAEAPTELFIDPSHTYRLRFTGKEKSLAPTPLGVTVRTLNDP
CCCCCCCEEEEEECCEEEEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCEEEEECCCC
IDADAMNRVYVRCGMVPAGVDVIWENHQHVPAVKYLLAVRDEDGAVVGTVTGVDHELLFN
CCCHHHCEEEEEECCCCCCCEEEECCCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEC
DPEHGSSLWTLAVDPAAGLPGVGGALTRALAEHFRSAGCAYMDLSVAHDNAAAISLYEKL
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHC
GFRRVPVLAIKRKNAINEPLFSPPPETVDDLNPYARIIADEALRRGIWVEVLDAETGEMR
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCEE
LTHGGRSVITRESLSEYTSAVAMCRCDDKRLTRRLVSEAGITVPRARLATFDEGDFAFLK
EECCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHH
EVGEVVVKPTRGEQGKGITVGVTAENGPDDLSAALARARAQYPDVLIEQRVPGDDLRLVV
HHHHHEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHEECCCCCCCEEEEE
IDGRVVAAALRLPPEVIGTGEHSVRDLIAAESRRRSAATGGESRIPLDELTEATVAEAGW
ECCCEEEHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
KLDDVLPQGTRLRVRRTANLHQGGTIHDVTAQVNSELCRVAVTAAEAIGIPVTGIDLLVP
CHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
DVTGADYAFIEANERPGLANHEPQPTAAAFIDFLFPGHPGQPQAWTPEESRFARG
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure 
TDPAEDHPSEAITLGLHDASPPEMVDAMAKDVELELGWGRLIFGQTFANSQELVEALRR
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCEEEEECCCEEEECHHHCCHHHHHHHHHH
EGPGRRDICIYARESHVVVAEAPTELFIDPSHTYRLRFTGKEKSLAPTPLGVTVRTLNDP
CCCCCCCEEEEEECCEEEEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCEEEEECCCC
IDADAMNRVYVRCGMVPAGVDVIWENHQHVPAVKYLLAVRDEDGAVVGTVTGVDHELLFN
CCCHHHCEEEEEECCCCCCCEEEECCCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEC
DPEHGSSLWTLAVDPAAGLPGVGGALTRALAEHFRSAGCAYMDLSVAHDNAAAISLYEKL
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHC
GFRRVPVLAIKRKNAINEPLFSPPPETVDDLNPYARIIADEALRRGIWVEVLDAETGEMR
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCEE
LTHGGRSVITRESLSEYTSAVAMCRCDDKRLTRRLVSEAGITVPRARLATFDEGDFAFLK
EECCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHH
EVGEVVVKPTRGEQGKGITVGVTAENGPDDLSAALARARAQYPDVLIEQRVPGDDLRLVV
HHHHHEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHEECCCCCCCEEEEE
IDGRVVAAALRLPPEVIGTGEHSVRDLIAAESRRRSAATGGESRIPLDELTEATVAEAGW
ECCCEEEHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
KLDDVLPQGTRLRVRRTANLHQGGTIHDVTAQVNSELCRVAVTAAEAIGIPVTGIDLLVP
CHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
DVTGADYAFIEANERPGLANHEPQPTAAAFIDFLFPGHPGQPQAWTPEESRFARG
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA