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The map label for this gene is ispE

Identifier: 41407074

GI number: 41407074

Start: 1014056

End: 1015006

Strand: Direct

Name: ispE

Synonym: MAP0976

Alternate gene names: 41407074

Gene position: 1014056-1015006 (Clockwise)

Preceding gene: 41407073

Following gene: 41407075

Centisome position: 21.0

GC content: 73.4

Gene sequence:

>951_bases
ATGTCTGACGGCAACACCGCCGCGGCTTGGGTGCCCACCGGGTCGGTCACCGTCCGGGTGCCCGGAAAAGTCAACCTCTA
CCTGGCCGTCGGCGATCGCCGCGAGGACGGCTACCACGAGCTGACCACGATTTTTCAGGCCGTCTCGCTGCTCGACGAGG
TGACCGTGCGCAACGCCGACGTGCTCTCGCTCGACATCGTCGGCGAGGGCGCCGACAAACTGCCCACCGACGAACGCAAC
CTGGCCTGGCAGGCCGCCGAGCTGATGGCCGACCACGTCGGCCGGGCGCCGGACGTGTCGATCATGATCGACAAGTCCAT
TCCGGTGGCCGGCGGGATGGCCGGCGGTAGCGCCGACGCGGCGGCGGTGCTGGTCGCGATGAACTCGCTGTGGGAGCTCA
ACGTGCCTCGCCGCGATCTGCGCATGCTCGCCGCGCGGCTGGGCAGCGACGTGCCGTTTGCGTTGCACGGCGGCACCGCG
CTGGGCACCGGCCGCGGCGAGGAGCTGGCGACGGTATTGTCCCGCAACACCTTTCACTGGGTGCTGGCGTTCGCCGACGG
CGAGCTGCTGACCCGCAAGGTGTTCGCCGAGCTGGACCGGCTGCGGCGCGCGGGCGATCCGCCGCGGCTGCCCGGGCCGG
GCCCGGTGCTGGCCGCGCTGGCCGCCGGCGACGCCGACCAGTTGGCGCCGCTGTTGGGCAATGAAATGCAGGCCGCCGCG
GTGAGCCTGAATCCCGGCCTGCGCCGCACCCTGCGCGCCGGCGTGCAGGCCGGCGCGCTGGCGGGCATCGTGTCGGGCTC
CGGCCCGACGTGTGCCTTCCTGTGCCCGTCGGCGGCCGCGGCGGTGGACGTCGGCACCGAGGTGTCCGGGGTGGGGGTGT
GCCGCACGGTGCGGGTCGCCAGCGGACCGGTGGCCGGTGCCCGCGTGGTCCCGGCACCGACCGAGGTGTGA

Upstream 100 bases:

>100_bases
CCGCCGGACAGGGCGCCCCGGCCCGCTGATCGCGGGACGCCCCGGGTCGCGATGCGTGTGCAAGGCGGCCGGCAGCGATA
GTCTGCTGCGGTGGGTAACG

Downstream 100 bases:

>100_bases
CACCCGAGCAGCGCGGCTGTGAAAAGAGCCCCAATTTCGCCATTAGTTTGGCGCTTCCCTAAGAGGAGTTTAAGATAATC
CGCGGTGACGGGAAGCGTTG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSDGNTAAAWVPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTIFQAVSLLDEVTVRNADVLSLDIVGEGADKLPTDERN
LAWQAAELMADHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTA
LGTGRGEELATVLSRNTFHWVLAFADGELLTRKVFAELDRLRRAGDPPRLPGPGPVLAALAAGDADQLAPLLGNEMQAAA
VSLNPGLRRTLRAGVQAGALAGIVSGSGPTCAFLCPSAAAAVDVGTEVSGVGVCRTVRVASGPVAGARVVPAPTEV

Sequences:

>Translated_316_residues
MSDGNTAAAWVPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTIFQAVSLLDEVTVRNADVLSLDIVGEGADKLPTDERN
LAWQAAELMADHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTA
LGTGRGEELATVLSRNTFHWVLAFADGELLTRKVFAELDRLRRAGDPPRLPGPGPVLAALAAGDADQLAPLLGNEMQAAA
VSLNPGLRRTLRAGVQAGALAGIVSGSGPTCAFLCPSAAAAVDVGTEVSGVGVCRTVRVASGPVAGARVVPAPTEV
>Mature_315_residues
SDGNTAAAWVPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTIFQAVSLLDEVTVRNADVLSLDIVGEGADKLPTDERNL
AWQAAELMADHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTAL
GTGRGEELATVLSRNTFHWVLAFADGELLTRKVFAELDRLRRAGDPPRLPGPGPVLAALAAGDADQLAPLLGNEMQAAAV
SLNPGLRRTLRAGVQAGALAGIVSGSGPTCAFLCPSAAAAVDVGTEVSGVGVCRTVRVASGPVAGARVVPAPTEV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=161, Percent_Identity=38.5093167701863, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786184, Length=286, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_MYCPA (Q741W1)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_959910.1
- ProteinModelPortal:   Q741W1
- SMR:   Q741W1
- EnsemblBacteria:   EBMYCT00000040035
- GeneID:   2721469
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP0976
- NMPDR:   fig|262316.1.peg.976
- GeneTree:   EBGT00050000016146
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK03188
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32460; Mature: 32329

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: ACT_SITE 23-23 ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGNTAAAWVPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTIFQAVSLLDEVTVRNAD
CCCCCEEEEEEECCEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VLSLDIVGEGADKLPTDERNLAWQAAELMADHVGRAPDVSIMIDKSIPVAGGMAGGSADA
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHH
AAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELATVLSRNTFHW
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHCCCEEE
VLAFADGELLTRKVFAELDRLRRAGDPPRLPGPGPVLAALAAGDADQLAPLLGNEMQAAA
EEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHCCCCEEEE
VSLNPGLRRTLRAGVQAGALAGIVSGSGPTCAFLCPSAAAAVDVGTEVSGVGVCRTVRVA
EECCHHHHHHHHHCCHHCCEEEEECCCCCEEEEECCCCHHHEECCCCCCCCCEEEEEEEC
SGPVAGARVVPAPTEV
CCCCCCCEECCCCCCC
>Mature Secondary Structure 
SDGNTAAAWVPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTIFQAVSLLDEVTVRNAD
CCCCEEEEEEECCEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VLSLDIVGEGADKLPTDERNLAWQAAELMADHVGRAPDVSIMIDKSIPVAGGMAGGSADA
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHH
AAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELATVLSRNTFHW
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHCCCEEE
VLAFADGELLTRKVFAELDRLRRAGDPPRLPGPGPVLAALAAGDADQLAPLLGNEMQAAA
EEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHCCCCEEEE
VSLNPGLRRTLRAGVQAGALAGIVSGSGPTCAFLCPSAAAAVDVGTEVSGVGVCRTVRVA
EECCHHHHHHHHHCCHHCCEEEEECCCCCEEEEECCCCHHHEECCCCCCCCCEEEEEEEC
SGPVAGARVVPAPTEV
CCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA