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The map label for this gene is pks16

Identifier: 41407075

GI number: 41407075

Start: 1015366

End: 1017000

Strand: Direct

Name: pks16

Synonym: MAP0977

Alternate gene names: 41407075

Gene position: 1015366-1017000 (Clockwise)

Preceding gene: 41407074

Following gene: 41407083

Centisome position: 21.02

GC content: 70.03

Gene sequence:

>1635_bases
GTGAGCAGGTTTACCGAGAAGATGTACCGCAATGCCCGGACCGCGAAGACGGGCATGGTCACCGGTGAACCCCACAACCC
GGTCCGTCATACCTGGGGCGAGGTGCACGAGCGTGCCCGCCGCATCGCCGGCGGCCTGGCCGCCGCGGGCATCGGGCCGG
GTGACGCGGTCGGCGTGCTGGCCGGTTTCCCGGTGGAGATCGCCCCGACCGCGCAGGGCCTGTGGATGCGCGGCGCCAGC
CTGACCATGCTGCACCAGCCCACCCCGCGCACCGACCTGGCGGTGTGGGCCGAGGACACCATGAACGTCATCGGCATGAT
CGAGGCGAAGGCAGTCGTCGTCTCGGAGCCCTTCCTGGTCGCCATCCCGGTCCTGCAGGAGAAGGACATCAAGGTCCTCA
CCGTCGCCGACCTGCTGGCGTCCGACCCGATCGACCCGGTCGAGGTCGGCGAGGACGACCTGGCGCTGATGCAGCTGACG
TCCGGCTCCACCGGGTCCCCCAAAGCCGTGCAGATCACCCACCGCAACATCTACTCCAACGCCGAGGCCATGTTCATCGG
CGCCCAGTACGACGTCGACAAGGACGTCATGGTCAGCTGGCTGCCCTGCTTCCACGACATGGGCATGGTCGGCTTCCTGA
CCATCCCGATGTACTTCGGCGCCGAGCTGGTCAAGGTCACCCCGATGGACTTCCTGCGGGACACCCTGCTGTGGGCCAAG
CTGATCGACAAGTACAAGGGCACCATGACCGCGGCGCCCAACTTCGCCTACGCGCTGCTGGCCAAGCGGCTGCGCCGGCA
GGCCAAGCCCGGCGACTTCGACCTGTCGACGCTGCGCTTCGCGCTGTCCGGCGCCGAGCCCGTCGAACCCGCCGACGTCG
AGGACCTGCTGGACGCGGGCAAGCCGTTCGGGCTCAAGCCGTCGGCGATCCTGCCGGCCTACGGCATGGCCGAAACCACG
CTGGCCGTGTCCTTCTCGGAGTGCAACGCCGGGCTGGTGGTCGACGAGGTGGACGCCGACCTGCTGGCCGCCCTGCGCCG
CGCCGTGCCGGCCACCAAGGGCAACACCCGCCGGCTGGCCACGCTCGGCCCGCTGCTGCAGGACCTGGAGGCGCGCATCG
TCGACGAGAACGGCGACGTGATGCCGCCGCGCGGGGTCGGCGTCATCGAGCTGCGCGGCGAGTCGGTGACGCCCGGCTAC
CTCACCATGGGCGGCTTCATCCCGGCCCAGGACGAGAACGGCTGGTATGACACCGGCGACCTCGGCTACCTGACCGAGGA
GGGGCACGTGGTGGTGTGCGGCCGGGTCAAGGACGTCATCATCATGGCCGGCCGCAACATCTATCCCACCGACATCGAGC
GGGCCGCCTGCCGTGTGGAGGGGGTGCGGCCCGGTTGCGCGGTGGCGGTGCGCCTGGACGCCGGCCACTCCCGCGAGACG
TTCGCGGTGGCGGTGGAGTCCAACGCCTTCCAGGACCCGGCCGAGGTGCGTCGCATCGAGCACCAGGTCGCCCGCGAGGT
GGTGGCCGAGGTCGACATGCGGCCGCGCAACGTGGTGGTGCTGGGCCCGGGGACCATCCCCAAGACGCCGTCGGGCAAGT
TGCGGCGATCCAACTCCGTCACCCTGGTGACTTAG

Upstream 100 bases:

>100_bases
CCGCCGAACTGTGCTTACCCGCCTGCGTCGCGGGGTGGCCCGGCGGGCGGAACATGAGATTTCGGGTGCTGGGGCCAGGG
CTGGAACCGAGGAGGTTTTC

Downstream 100 bases:

>100_bases
TCAGGCGAGCAGACACAAAAGCACCCAAAACATCGGCGTGTCGGGTGCTTTCGCGTCTGCTCGCGGTTCCTGCGCTACCA
GGCGTGCACCCGGTTCTGGG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSRFTEKMYRNARTAKTGMVTGEPHNPVRHTWGEVHERARRIAGGLAAAGIGPGDAVGVLAGFPVEIAPTAQGLWMRGAS
LTMLHQPTPRTDLAVWAEDTMNVIGMIEAKAVVVSEPFLVAIPVLQEKDIKVLTVADLLASDPIDPVEVGEDDLALMQLT
SGSTGSPKAVQITHRNIYSNAEAMFIGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAGKPFGLKPSAILPAYGMAETT
LAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLATLGPLLQDLEARIVDENGDVMPPRGVGVIELRGESVTPGY
LTMGGFIPAQDENGWYDTGDLGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAACRVEGVRPGCAVAVRLDAGHSRET
FAVAVESNAFQDPAEVRRIEHQVAREVVAEVDMRPRNVVVLGPGTIPKTPSGKLRRSNSVTLVT

Sequences:

>Translated_544_residues
MSRFTEKMYRNARTAKTGMVTGEPHNPVRHTWGEVHERARRIAGGLAAAGIGPGDAVGVLAGFPVEIAPTAQGLWMRGAS
LTMLHQPTPRTDLAVWAEDTMNVIGMIEAKAVVVSEPFLVAIPVLQEKDIKVLTVADLLASDPIDPVEVGEDDLALMQLT
SGSTGSPKAVQITHRNIYSNAEAMFIGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAGKPFGLKPSAILPAYGMAETT
LAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLATLGPLLQDLEARIVDENGDVMPPRGVGVIELRGESVTPGY
LTMGGFIPAQDENGWYDTGDLGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAACRVEGVRPGCAVAVRLDAGHSRET
FAVAVESNAFQDPAEVRRIEHQVAREVVAEVDMRPRNVVVLGPGTIPKTPSGKLRRSNSVTLVT
>Mature_543_residues
SRFTEKMYRNARTAKTGMVTGEPHNPVRHTWGEVHERARRIAGGLAAAGIGPGDAVGVLAGFPVEIAPTAQGLWMRGASL
TMLHQPTPRTDLAVWAEDTMNVIGMIEAKAVVVSEPFLVAIPVLQEKDIKVLTVADLLASDPIDPVEVGEDDLALMQLTS
GSTGSPKAVQITHRNIYSNAEAMFIGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAKL
IDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAGKPFGLKPSAILPAYGMAETTL
AVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLATLGPLLQDLEARIVDENGDVMPPRGVGVIELRGESVTPGYL
TMGGFIPAQDENGWYDTGDLGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAACRVEGVRPGCAVAVRLDAGHSRETF
AVAVESNAFQDPAEVRRIEHQVAREVVAEVDMRPRNVVVLGPGTIPKTPSGKLRRSNSVTLVT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=329, Percent_Identity=24.6200607902736, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788107, Length=343, Percent_Identity=27.6967930029155, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI145693145, Length=390, Percent_Identity=27.9487179487179, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789201, Length=411, Percent_Identity=26.2773722627737, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=457, Percent_Identity=24.72647702407, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=326, Percent_Identity=26.3803680981595, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=408, Percent_Identity=24.5098039215686, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=481, Percent_Identity=21.6216216216216, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=481, Percent_Identity=21.6216216216216, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=481, Percent_Identity=21.6216216216216, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=405, Percent_Identity=23.9506172839506, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=333, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=332, Percent_Identity=26.8072289156627, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI20130357, Length=512, Percent_Identity=24.8046875, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=537, Percent_Identity=22.9050279329609, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859661, Length=415, Percent_Identity=26.0240963855422, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=308, Percent_Identity=24.025974025974, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648257, Length=419, Percent_Identity=22.673031026253, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58694; Mature: 58563

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFTEKMYRNARTAKTGMVTGEPHNPVRHTWGEVHERARRIAGGLAAAGIGPGDAVGVL
CCHHHHHHHHCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHH
AGFPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWAEDTMNVIGMIEAKAVVVSEPFLV
CCCCEEECCCCCCEEECCCEEEEEECCCCCCCEEEEECHHHCCCEEEEEEEEEEECCEEE
AIPVLQEKDIKVLTVADLLASDPIDPVEVGEDDLALMQLTSGSTGSPKAVQITHRNIYSN
EEECCCCCCCEEEEEHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCCCEEEEEECHHHCC
AEAMFIGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
CCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAG
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCHHHHHHCC
KPFGLKPSAILPAYGMAETTLAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEHH
TLGPLLQDLEARIVDENGDVMPPRGVGVIELRGESVTPGYLTMGGFIPAQDENGWYDTGD
HHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCEEEECCEEECCCCCCCCCCCC
LGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAACRVEGVRPGCAVAVRLDAGHSRET
CCEEECCCCEEEECCCCEEEEEECCCCCCCCHHHHHHEECCCCCCEEEEEEECCCCCCCE
FAVAVESNAFQDPAEVRRIEHQVAREVVAEVDMRPRNVVVLGPGTIPKTPSGKLRRSNSV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
TLVT
EEEC
>Mature Secondary Structure 
SRFTEKMYRNARTAKTGMVTGEPHNPVRHTWGEVHERARRIAGGLAAAGIGPGDAVGVL
CHHHHHHHHCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHH
AGFPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWAEDTMNVIGMIEAKAVVVSEPFLV
CCCCEEECCCCCCEEECCCEEEEEECCCCCCCEEEEECHHHCCCEEEEEEEEEEECCEEE
AIPVLQEKDIKVLTVADLLASDPIDPVEVGEDDLALMQLTSGSTGSPKAVQITHRNIYSN
EEECCCCCCCEEEEEHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCCCEEEEEECHHHCC
AEAMFIGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
CCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAG
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCHHHHHHCC
KPFGLKPSAILPAYGMAETTLAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEHH
TLGPLLQDLEARIVDENGDVMPPRGVGVIELRGESVTPGYLTMGGFIPAQDENGWYDTGD
HHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCEEEECCEEECCCCCCCCCCCC
LGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAACRVEGVRPGCAVAVRLDAGHSRET
CCEEECCCCEEEECCCCEEEEEECCCCCCCCHHHHHHEECCCCCCEEEEEEECCCCCCCE
FAVAVESNAFQDPAEVRRIEHQVAREVVAEVDMRPRNVVVLGPGTIPKTPSGKLRRSNSV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
TLVT
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]