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The map label for this gene is hcaE [H]

Identifier: 41407031

GI number: 41407031

Start: 962576

End: 963805

Strand: Direct

Name: hcaE [H]

Synonym: MAP0933

Alternate gene names: 41407031

Gene position: 962576-963805 (Clockwise)

Preceding gene: 41407029

Following gene: 41407032

Centisome position: 19.93

GC content: 66.75

Gene sequence:

>1230_bases
TTGACAATACAAACGGTGATGATTGTATGGTCTGGGAGGTTGACGGCGAAGGAGAAGCGAGTGGCCCCCATCAACAGCCC
GGTGAGCGGCACCGACGAGGCGTTGACGCGGCGGGGGCTGCGTCACGCCCTGGACAAGACGACCGACCTGGCCGAGCGGG
AACTGCGGGTGCCGCTGCACTACTACCGCGACCCCAAGATCACCGAGATCGAGGAGGCGCAGATCCTGCGTCGGGTCCCG
CTGGCCATCGTCCCGTCGGCGCAGCTACCGAACACCAACGACTACGTGGTGCGCTCGGTGCTGGGCGATTCCCTGCTGGT
GACCCGCGACCGTTCCGGGGCCAGTCACGTCCTGCTCAACTACTGCCGGCACCGGGGCGCGATGCCGGCCTGCGGCTCGG
GCAACACCGCGCGGTTCGTGTGCCCGTACCACGCCTGGACGTACAAGAACACCGGCGAACTGTTCTCGGTGCCCGGCAAG
GCCGGCTTCGACTCGATGAACACCAAGGACTACGGCCTGGTGGAGCTGCCCTCCGAGGAGCGGCACGGCTTCATCTGGGC
GGTGCTGACCGCCGACGCGACCATCGACCTCGACGCGCACCTGGGCGACTTCGGAGCCGAACTGGCGCTGTGGAATTACT
CCTCCTACGGCTATCACACCCAGCGCGAATTCACCTCCGAGGTGTCCTGGAAGGGCGCCCTGGAGGCATTCGCCGAGGGC
TACCACTTCCCGTACGTCCACGGGCAGAGCCTGATCGGGCAGAACACCCTGGCCAACACCATGGTCTACGACGAGTTCGG
CAAGCATCACCGGATCGGCTTCCCGTTCACCTGGATCACCAACGCCGCCACCGACCCTGCGGCGTCGCTGGAACCGCTGG
CCAACATGGGCGTGATCTATTGGGTGTATCCGAACCTCATCCTGGCCAACAGCCCGGTCGGGCTGGAGATCATCGACATG
CTGCCCGCCGGTGCGCCGACACGCTGCACGGTGCGGCACAGCTGGATGGCCCGCGTCCCCGCCGCCGACGACGAGATGCG
CGCGGCCTACGACGCGGTCTTCGAAGGCGTGCACGCCGCGGTGCGCGACGAGGACTTCGCCATGCTGCCCCAGTGCGGCG
AGGGCGTCCGGCACGGCCAGCACGACCACATGATCATTGGGCGCAACGAAATCGCCGTCCAGCACATGATCAGGGTGTTC
GCCCACGAGCTGGGGGTGGCACTGGCCTGA

Upstream 100 bases:

>100_bases
GTCAGGATGCGCGCAACCGCCTCCTCGTCGTCGGCCGGGATGTCACCCGACCAGCCGTGCCGGCGCATTGCGTGATCGTC
GCACACACCGGGCCGCGGCC

Downstream 100 bases:

>100_bases
GTCGAGGCGGTTGGAGTGGCCGCAGGCCGGCGCGGGTACAGATGCTCTGAACCTGATCGAGAGGATCCGATGGCGCACCG
CGACCGCGACTCGACCGACG

Product: hypothetical protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTIQTVMIVWSGRLTAKEKRVAPINSPVSGTDEALTRRGLRHALDKTTDLAERELRVPLHYYRDPKITEIEEAQILRRVP
LAIVPSAQLPNTNDYVVRSVLGDSLLVTRDRSGASHVLLNYCRHRGAMPACGSGNTARFVCPYHAWTYKNTGELFSVPGK
AGFDSMNTKDYGLVELPSEERHGFIWAVLTADATIDLDAHLGDFGAELALWNYSSYGYHTQREFTSEVSWKGALEAFAEG
YHFPYVHGQSLIGQNTLANTMVYDEFGKHHRIGFPFTWITNAATDPAASLEPLANMGVIYWVYPNLILANSPVGLEIIDM
LPAGAPTRCTVRHSWMARVPAADDEMRAAYDAVFEGVHAAVRDEDFAMLPQCGEGVRHGQHDHMIIGRNEIAVQHMIRVF
AHELGVALA

Sequences:

>Translated_409_residues
MTIQTVMIVWSGRLTAKEKRVAPINSPVSGTDEALTRRGLRHALDKTTDLAERELRVPLHYYRDPKITEIEEAQILRRVP
LAIVPSAQLPNTNDYVVRSVLGDSLLVTRDRSGASHVLLNYCRHRGAMPACGSGNTARFVCPYHAWTYKNTGELFSVPGK
AGFDSMNTKDYGLVELPSEERHGFIWAVLTADATIDLDAHLGDFGAELALWNYSSYGYHTQREFTSEVSWKGALEAFAEG
YHFPYVHGQSLIGQNTLANTMVYDEFGKHHRIGFPFTWITNAATDPAASLEPLANMGVIYWVYPNLILANSPVGLEIIDM
LPAGAPTRCTVRHSWMARVPAADDEMRAAYDAVFEGVHAAVRDEDFAMLPQCGEGVRHGQHDHMIIGRNEIAVQHMIRVF
AHELGVALA
>Mature_408_residues
TIQTVMIVWSGRLTAKEKRVAPINSPVSGTDEALTRRGLRHALDKTTDLAERELRVPLHYYRDPKITEIEEAQILRRVPL
AIVPSAQLPNTNDYVVRSVLGDSLLVTRDRSGASHVLLNYCRHRGAMPACGSGNTARFVCPYHAWTYKNTGELFSVPGKA
GFDSMNTKDYGLVELPSEERHGFIWAVLTADATIDLDAHLGDFGAELALWNYSSYGYHTQREFTSEVSWKGALEAFAEGY
HFPYVHGQSLIGQNTLANTMVYDEFGKHHRIGFPFTWITNAATDPAASLEPLANMGVIYWVYPNLILANSPVGLEIIDML
PAGAPTRCTVRHSWMARVPAADDEMRAAYDAVFEGVHAAVRDEDFAMLPQCGEGVRHGQHDHMIIGRNEIAVQHMIRVFA
HELGVALA

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788103, Length=333, Percent_Identity=23.4234234234234, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788888, Length=212, Percent_Identity=30.188679245283, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 45443; Mature: 45312

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQTVMIVWSGRLTAKEKRVAPINSPVSGTDEALTRRGLRHALDKTTDLAERELRVPLH
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YYRDPKITEIEEAQILRRVPLAIVPSAQLPNTNDYVVRSVLGDSLLVTRDRSGASHVLLN
CCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHH
YCRHRGAMPACGSGNTARFVCPYHAWTYKNTGELFSVPGKAGFDSMNTKDYGLVELPSEE
HHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCEEECCCCCCCCCCCCCCCCEEECCCCC
RHGFIWAVLTADATIDLDAHLGDFGAELALWNYSSYGYHTQREFTSEVSWKGALEAFAEG
CCEEEEEEEECCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCC
YHFPYVHGQSLIGQNTLANTMVYDEFGKHHRIGFPFTWITNAATDPAASLEPLANMGVIY
CCCCEECCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCEEE
WVYPNLILANSPVGLEIIDMLPAGAPTRCTVRHSWMARVPAADDEMRAAYDAVFEGVHAA
EECCCEEEECCCCCEEEEECCCCCCCCEEEEECCHHEECCCCCHHHHHHHHHHHHHHHHH
VRDEDFAMLPQCGEGVRHGQHDHMIIGRNEIAVQHMIRVFAHELGVALA
HCCCCCEECCHHCHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIQTVMIVWSGRLTAKEKRVAPINSPVSGTDEALTRRGLRHALDKTTDLAERELRVPLH
CEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YYRDPKITEIEEAQILRRVPLAIVPSAQLPNTNDYVVRSVLGDSLLVTRDRSGASHVLLN
CCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHH
YCRHRGAMPACGSGNTARFVCPYHAWTYKNTGELFSVPGKAGFDSMNTKDYGLVELPSEE
HHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCEEECCCCCCCCCCCCCCCCEEECCCCC
RHGFIWAVLTADATIDLDAHLGDFGAELALWNYSSYGYHTQREFTSEVSWKGALEAFAEG
CCEEEEEEEECCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCC
YHFPYVHGQSLIGQNTLANTMVYDEFGKHHRIGFPFTWITNAATDPAASLEPLANMGVIY
CCCCEECCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCEEE
WVYPNLILANSPVGLEIIDMLPAGAPTRCTVRHSWMARVPAADDEMRAAYDAVFEGVHAA
EECCCEEEECCCCCEEEEECCCCCCCCEEEEECCHHEECCCCCHHHHHHHHHHHHHHHHH
VRDEDFAMLPQCGEGVRHGQHDHMIIGRNEIAVQHMIRVFAHELGVALA
HCCCCCEECCHHCHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]