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The map label for this gene is nemA [H]

Identifier: 52842927

GI number: 52842927

Start: 3074894

End: 3075970

Strand: Reverse

Name: nemA [H]

Synonym: lpg2722

Alternate gene names: 52842927

Gene position: 3075970-3074894 (Counterclockwise)

Preceding gene: 52842928

Following gene: 52842923

Centisome position: 90.53

GC content: 39.18

Gene sequence:

>1077_bases
ATGACATGCAGGAGATTTATCATGGTTAACTCCGATTTAATTTTAAGTCCTTATCAGCTTAATGATAAAATTACACTAAA
AAACAGAGTCATTATGGCTCCTATGACTCGTAATATGGCTAACGATGATTTCAGTCCAACCAGGCAGATGGCAGATTATT
ATGCTCGAAGGGCTGATGCCGGGTTGATTATTACAGAAGGCACTATTATTAGCCCTGATGGTCAGGGTTATAGCAATGCT
CCAGGAATATTTACGCAAACCCATATTGACGGTTGGAAAATGGTAACTGATGCTGTGCATGAACGGGGAGGGAAAATATT
TTTGCAAATTTGGCATGTTGGCAGGGTATCTCATCCGATTTTTTTACATGGTAACTTACCGATTTCTGCTTCAGAAACGA
TGATGACAGGTAAGGTCCGGCGTGCCGATGATCTTTATTATGGAAAATCAAGGGCGGCTACATTAGAGGAAATTAATGAG
TTAATCCAAAACTATGCGTTGGCTGCAAGAAGAGCGATGATAGCCGGGTTTGATGGCATTGAGATTCATGGTGCCAATGG
TTATTTGATTGATCAGTTTTTACATTACCATACCAATCAACGTCATGATCAGTATGGACAATATCCAGAGAATATGGGGC
GATTTGCGCTGGAAGTGGTAAAAGCCTGTATCAATGAAATAGGTGGGCAATCTGTAGGATTGCGCCTTTCTCCCGGAGCT
TATCTTAATGAAATTGTCGGAGATGAGCGAGATGCCATGGTATTTCGATATGTATTGGAAGAATTGAATCACTATGGAAT
AGCTTATGTGCATACAGGTAATTTTGATGACGGTATTCAATTCAAAGAATTAGGGTTCAAAACGATGACAGAATTTATGA
GAGCTCATTTTAAAGGAACCTTAATTGCCAGCGGTAGCTATACGCTTCACCAGGCAAGAAAAGATATTCAAAATAAAAAA
TACGATTTAATTGCTTTGGGAAGGCCATTTATTGCTAATCCCGATTTAATGAATCGTATCAAGTGGAATGCCGATTTTCT
ACCTTATGAAAACAGAATGTTGGACACCTTGTTTTAG

Upstream 100 bases:

>100_bases
ATGCAAGCCTAATTAATGAATTTATAAATGAATTGAAAGTTATGATTTAAAAAATTTTGCTTGAATAAATCGAATATTTG
CGATATTCCAATGTATTGTT

Downstream 100 bases:

>100_bases
GCACTTGAGCGGGGCATTCAATTACCTTTCTTGCTTTCTGTATTACCGGAAAATTTTGGAATGAAGTGATGAGAAAGGCA
CAAGGAGATGATGAGAAAGC

Product: Oye family NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTCRRFIMVNSDLILSPYQLNDKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEGTIISPDGQGYSNA
PGIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINE
LIQNYALAARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGA
YLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSYTLHQARKDIQNKK
YDLIALGRPFIANPDLMNRIKWNADFLPYENRMLDTLF

Sequences:

>Translated_358_residues
MTCRRFIMVNSDLILSPYQLNDKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEGTIISPDGQGYSNA
PGIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINE
LIQNYALAARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGA
YLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSYTLHQARKDIQNKK
YDLIALGRPFIANPDLMNRIKWNADFLPYENRMLDTLF
>Mature_357_residues
TCRRFIMVNSDLILSPYQLNDKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEGTIISPDGQGYSNAP
GIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINEL
IQNYALAARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGAY
LNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSYTLHQARKDIQNKKY
DLIALGRPFIANPDLMNRIKWNADFLPYENRMLDTLF

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=353, Percent_Identity=39.0934844192635, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789463, Length=330, Percent_Identity=29.3939393939394, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=372, Percent_Identity=27.1505376344086, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=345, Percent_Identity=28.695652173913, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=370, Percent_Identity=27.027027027027, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=370, Percent_Identity=25.4054054054054, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=363, Percent_Identity=26.7217630853994, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=343, Percent_Identity=27.4052478134111, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=350, Percent_Identity=35.1428571428571, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325086, Length=340, Percent_Identity=36.4705882352941, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40735; Mature: 40604

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCRRFIMVNSDLILSPYQLNDKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADA
CCCEEEEEECCCEEECCEECCCEEEECCEEEECCHHCCCCCCCCCHHHHHHHHHHHHCCC
GLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPI
CEEEECCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCE
FLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYALAARRAMIAGFDGI
EEECCCCCCCCCHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
EIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGA
EEECCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
YLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGT
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHCCE
LIASGSYTLHQARKDIQNKKYDLIALGRPFIANPDLMNRIKWNADFLPYENRMLDTLF
EEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
TCRRFIMVNSDLILSPYQLNDKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADA
CCEEEEEECCCEEECCEECCCEEEECCEEEECCHHCCCCCCCCCHHHHHHHHHHHHCCC
GLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPI
CEEEECCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCE
FLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYALAARRAMIAGFDGI
EEECCCCCCCCCHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
EIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGA
EEECCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
YLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGT
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHCCE
LIASGSYTLHQARKDIQNKKYDLIALGRPFIANPDLMNRIKWNADFLPYENRMLDTLF
EEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]