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The map label for this gene is pheS

Identifier: 52842917

GI number: 52842917

Start: 3063296

End: 3064324

Strand: Reverse

Name: pheS

Synonym: lpg2711

Alternate gene names: 52842917

Gene position: 3064324-3063296 (Counterclockwise)

Preceding gene: 52842918

Following gene: 52842916

Centisome position: 90.19

GC content: 40.82

Gene sequence:

>1029_bases
GTGGGTAGAACTATGTTGGTACTTATAAACACCATACAAGAACAAGCTTCAGAAGCAATCAAACAAGCCACGGATATAGT
TGCTCTTGAACAAATTCGTGTTGATTTTTTGGGCAAGAAAGGCAAGCTCACCGAATTGCTAAAAGGACTTGCTAATTTGT
CTGCAGAGGAAAAACCCAAGGTAGGTCAATTGGTCAATCAAGCAAAGCAGGGAATTAGTGCTTTGATTGAAACAAAAATG
ATCGAGTTGAAAGAAAAGCAATTATTAGCCAAGCTGGCAGCAGAGCAAATCGACGTTACTCTTCCCGGTCGCAATCATTC
CACAGGTTCCCTACATCCTGTTACTCAGGTAAAGCATAGAATTAATGATTATTTTAGTCGTTTAGGATTTGATATTGTTG
AAGGTCCTGAAATTGAAACAGAATTTTATAATTTTGAAGCATTAAATATCCCTGGCCATCATCCTGCACGTGCGATGCAC
GATACGTTTTATTTTGGAGACGGACGCTTGTTGAGGACACACACCTCTCCTGTACAAATCAGGACAATGGAGCAGCGTAA
GCCACCTTTTCGTTTAATTGCTCCTGGCCGAGTTTATCGTTGTGATTCGGATGTAACCCATACCCCTATGTTTCATCAAG
TGGAGGGATTACTCATTGATAAGCAAGCCACTTTGGCCGGGTTAAAGGGTTTGTTGCAAGATTTCTTCGCTTATTTTTTT
GGTCGGGAATTGGCATTAAGATTCAGACCTTCCTATTTCCCTTTTACCGAACCTTCCGCAGAAGTGGATATAGAATGTAC
TCAATGTAATGGTAAGGGTTGCCGCTCTTGCAAGTTTACAGGCTGGCTTGAGGTATTGGGTTGTGGTATGGTCCACCCTA
ACGTTTTAATCGCAGTGAACATTGATCCGAATGAGTATCATGGATGGGCTTTTGGCATGGGTATGGACAGATTGGCAATG
CTTTATTATGGCATAGATGATTTGCGTATGTTATTTGAAAATGATTTAACTTTTTTACGGCAGTTTTAA

Upstream 100 bases:

>100_bases
TGCCTTTCGTTTATAAATCTCAATTCGGAATAGCAATCTTAAATAAAAGCCGGTTTGTAAAATAAAAATTATTGAGGAGG
CTAAGGCCTCCTTTTTACTT

Downstream 100 bases:

>100_bases
CCATTATAAAAATAATGAGTCCCGGATTTTATTTTTATGTTTCTTTATGATTGTCTTTCATATGGTACATAAATGATACA
GATTAGACCAAATTGTAGTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MGRTMLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQLVNQAKQGISALIETKM
IELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMH
DTFYFGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFF
GRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAM
LYYGIDDLRMLFENDLTFLRQF

Sequences:

>Translated_342_residues
MGRTMLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQLVNQAKQGISALIETKM
IELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMH
DTFYFGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFF
GRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAM
LYYGIDDLRMLFENDLTFLRQF
>Mature_341_residues
GRTMLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPKVGQLVNQAKQGISALIETKMI
ELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHD
TFYFGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFFG
RELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAML
YYGIDDLRMLFENDLTFLRQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=290, Percent_Identity=32.0689655172414, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4758340, Length=272, Percent_Identity=31.6176470588235, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=57.7981651376147, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=30.2919708029197, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=30.2919708029197, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=285, Percent_Identity=31.9298245614035, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=271, Percent_Identity=29.8892988929889, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=40.5940594059406, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18858079, Length=307, Percent_Identity=28.0130293159609, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137424, Length=299, Percent_Identity=28.0936454849498, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_LEGPH (Q5ZS08)

Other databases:

- EMBL:   AE017354
- RefSeq:   YP_096716.1
- ProteinModelPortal:   Q5ZS08
- SMR:   Q5ZS08
- STRING:   Q5ZS08
- GeneID:   3079427
- GenomeReviews:   AE017354_GR
- KEGG:   lpn:lpg2711
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   LPNE272624:LPG2711-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38724; Mature: 38593

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTMLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPK
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
VGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHH
INDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHDTFYFGDGRLLRTHTSPVQI
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHCEEEECCCEEEEECCCCEEE
RTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFF
EEHHHCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECHHHHHHHHHHHHHHHHHHH
GRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVN
CCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEE
IDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTFLRQF
ECCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRTMLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKGLANLSAEEKPK
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
VGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNHSTGSLHPVTQVKHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHH
INDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHDTFYFGDGRLLRTHTSPVQI
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHCEEEECCCEEEEECCCCEEE
RTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQATLAGLKGLLQDFFAYFF
EEHHHCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECHHHHHHHHHHHHHHHHHHH
GRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTGWLEVLGCGMVHPNVLIAVN
CCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEE
IDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTFLRQF
ECCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA