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The map label for this gene is murF

Identifier: 52842824

GI number: 52842824

Start: 2954902

End: 2956287

Strand: Reverse

Name: murF

Synonym: lpg2618

Alternate gene names: 52842824

Gene position: 2956287-2954902 (Counterclockwise)

Preceding gene: 52842825

Following gene: 52842823

Centisome position: 87.01

GC content: 43.0

Gene sequence:

>1386_bases
GTGAGCCTGAGTGTTAGTACACTAGTCTTTTCGACATCACAAAATACTATGAACCTAATTACTATTGCGTCACTTCTTTC
TCAGTCTTGCCAGATTAATGCCGACATTACTGGTGTTAGTATAGATACCCGCACATTAAAGCCAGGAAATTTATTTTTTG
CGATAGTGGGGGAACGCTTTGATGGTCATGATTTTATTAAAGAAGCCGAGGCTAAAGGCGCTTCAGCTGCTGTAGTTAAT
CGATCGATTGACGGTGTTCATATTCCTCAATTCGTGGTTGCCAGTCCTTTAATGGCTTTGGCCAAAATTGCTGCGGCACA
TCGACAGGAAATGAGTTGCCCTATTATTGCTTTAACAGGGTCAAATGGAAAAACCACTGTCAAAGAGATGATAGCGACTA
TTTTACCGCAGCCATCACATGCTACGAAAGGCAATTTAAATAATCACATAGGGGTTCCTTTAAGCATGTTAGCTTTAAAT
AAGCAGCACCGCTATGCTGTTTTTGAGTTGGGAGCCAATCATCCCGGAGAAATAGCACATACGGTAGGGATAGTGCAGCC
TGATGTGTCTTTAATTAATAATATTGCCCCGGCACATGTCGAAGGCTTTGGATCAATAGACGGAGTCGCTCGTTCCAAAG
GTGAAATCCATCAAGGCTTATCCCCGACTGGTGTTGCTATAGTCAATGATGATGATGCCTACGCTCACTTTTGGGATGAT
TTGATTGCTGATAAAAAAATATTACGTTTTTCCTCACTTCATCCTGCAGATATTTACGCCCAGGATATACGCTTGGATAC
CCATGGGCGAGCTCATTTTACATTGGTTTTACCTAATAGCAGAGTGGAGATTGAGTTACAAGTACCAGGTTTGCACAATG
TCCGAAATGCTTTGGCTGCCGCAGCTTGTTGTTATGCTGTAGGAATATCAGGCAATGACATTCAAAGAGGACTGGCTCAT
TTTGGTGGTGTAAAAGGTCGGATGACGATGTTGTCAGGGCAAAATCAATCAACAGTTATTGATGATACATACAATGCTAA
TTTACGCTCTGTCTTAACAGCCCTGGAAGTTTTATCTGGGCGCCCAGGAAGAAAAATTTTTGTATTCGGTGATATGGGAG
AGTTGGGTGAATGGACGACACAACATCACAGAGAAGTTGGCGTAGCGGCTCGCCAGCTGGGAATAGATAAGTTGATGAGT
TGCGGTTTACATAGTGAACTGGCATCCCAGGCGTTTGGTGAGGGAGGTCAGCATTTTTGCAGTCAAGAAGAGTTGATTGA
AGTGGTAAAAAATGATTTAACACCTGATACCACGGTATTGGTAAAAGGATCCAGGTCCAGTGCTATGGAAAATGTTGTGC
ACCGGTTGGTTCAGGTAGAGGGCTAA

Upstream 100 bases:

>100_bases
GGTTAATGTCTGATAACTTATGAATCGGATTGTAATAAACTTAAAATTCTTGACTTTTTAGTTATATTCTTATCTATCTA
TTCTCAAACCATCTTGAACT

Downstream 100 bases:

>100_bases
CCTCTTCATTGAATCGACAGGAATGTGGTATGCTTGGCCACTTTTTTGCCCCATTAAAGGAATCATGCTCAATAAAAGTC
ATTGAGCATAATTTTAAGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSLSVSTLVFSTSQNTMNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERFDGHDFIKEAEAKGASAAVVN
RSIDGVHIPQFVVASPLMALAKIAAAHRQEMSCPIIALTGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALN
KQHRYAVFELGANHPGEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYAHFWDD
LIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVRNALAAAACCYAVGISGNDIQRGLAH
FGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGVAARQLGIDKLMS
CGLHSELASQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG

Sequences:

>Translated_461_residues
MSLSVSTLVFSTSQNTMNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERFDGHDFIKEAEAKGASAAVVN
RSIDGVHIPQFVVASPLMALAKIAAAHRQEMSCPIIALTGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALN
KQHRYAVFELGANHPGEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYAHFWDD
LIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVRNALAAAACCYAVGISGNDIQRGLAH
FGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGVAARQLGIDKLMS
CGLHSELASQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG
>Mature_460_residues
SLSVSTLVFSTSQNTMNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERFDGHDFIKEAEAKGASAAVVNR
SIDGVHIPQFVVASPLMALAKIAAAHRQEMSCPIIALTGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNK
QHRYAVFELGANHPGEIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYAHFWDDL
IADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVRNALAAAACCYAVGISGNDIQRGLAHF
GGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGVAARQLGIDKLMSC
GLHSELASQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=430, Percent_Identity=45.8139534883721, Blast_Score=325, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49373; Mature: 49242

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSVSTLVFSTSQNTMNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
CCCEEEEEEEECCCCCHHHHHHHHHHHCCCEECCCEEEEEEECEECCCCCEEEEEECCCC
DGHDFIKEAEAKGASAAVVNRSIDGVHIPQFVVASPLMALAKIAAAHRQEMSCPIIALTG
CCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
SNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPGEIAH
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHEEHCCCCCCEEEEEECCCCCCCHHH
TVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYAHFWDD
HHEECCCCHHHHHCCCCCHHCCCCCCCHHHHCCCHHHCCCCCCCEEEECCCCCHHHHHHH
LIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVRNALAA
HHHCHHHHHHCCCCCHHHHHHHEEEECCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHH
AACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSG
HHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHCC
RPGRKIFVFGDMGELGEWTTQHHREVGVAARQLGIDKLMSCGLHSELASQAFGEGGQHFC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCHHHC
SQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG
CHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLSVSTLVFSTSQNTMNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
CCEEEEEEEECCCCCHHHHHHHHHHHCCCEECCCEEEEEEECEECCCCCEEEEEECCCC
DGHDFIKEAEAKGASAAVVNRSIDGVHIPQFVVASPLMALAKIAAAHRQEMSCPIIALTG
CCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
SNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPGEIAH
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHEEHCCCCCCEEEEEECCCCCCCHHH
TVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAYAHFWDD
HHEECCCCHHHHHCCCCCHHCCCCCCCHHHHCCCHHHCCCCCCCEEEECCCCCHHHHHHH
LIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLHNVRNALAA
HHHCHHHHHHCCCCCHHHHHHHEEEECCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHH
AACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRSVLTALEVLSG
HHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHCC
RPGRKIFVFGDMGELGEWTTQHHREVGVAARQLGIDKLMSCGLHSELASQAFGEGGQHFC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCHHHC
SQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG
CHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]