| Definition | Geobacter sulfurreducens PCA chromosome, complete genome. |
|---|---|
| Accession | NC_002939 |
| Length | 3,814,139 |
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The map label for this gene is metH [H]
Identifier: 39998012
GI number: 39998012
Start: 3219940
End: 3222354
Strand: Reverse
Name: metH [H]
Synonym: GSU2921
Alternate gene names: 39998012
Gene position: 3222354-3219940 (Counterclockwise)
Preceding gene: 39998013
Following gene: 39998010
Centisome position: 84.48
GC content: 66.38
Gene sequence:
>2415_bases ATGAAACAACCCTTTCTTCAGGCTATTGCCGAGCGGGTCCTCGTCCTTGACGGCGCCATGGGGACCATGCTCCAGGAGCG GGGGCTCAGACCCGGCCAGTCCCCCGAGGAACTCAACCTGACCCTGCCCGAGGTGGTTGCCGGCGTCCACCGGGAATACC TGGATGCCGGCGCCGATATCATCGTTACAAATACATTTGGCGGCAGCCGTGCCAAGCTGGAGCACTATGGCCTTCAGGAC CGGGTCGCCGAGATCAATGCCCGGGCCGTGGCCATCGCGCGGGAGGTCTGCGGCGACCGGGCCTACGTGGCCGCATCAAT CGGCCCCACGGGGCAGTTCGTGGAGCCGGTGGGAGATGTGTCGTTCGACGAGATGGCCGCCATCTTCCGCGAGCAGGCAC AGGCGCTCATCAACGCCGGGGCGGACCTGATCACCCTGGAGACCTTTCTCGACATCAAGGAAATCCGTGCTGCGGTCATC GCCATTCGCGAGATTTCGCCGGAAACCCCCGTCATTGCCCAGCTTACCTTTGACAACGAGGGGCGGACCGTCCTCGGCAC CTCGCCCGAGGCCGCCGCGGTGACCCTGGAGGCGGCCGGTGCCGACATCGTCGGCTCCAACTGCGGCCTGGGTCCCGACG GCATCTGCGATGTCATGGCGGCCATGCGACGGGTTACGCGGCTTCCTCTCATTTCCCAGGCCAACGCGGGGCTGCCGACG CTTGTGGACGGCGCCACCGTCTTCCCCGGCACTCCCGACGACATGACGGCGTTCCATGACCGGTTGCTGGATCTGAACGT CCGGGTCATCGGCGGCTGCTGCGGCACCACGCCCGCCCACATCCGCGCCATCAGGGAGGCCCTGGCCCTGCGGGATCAGT CCCTGCGCGCCTGTGGCCCGGCCGAGGGAGTTACCTTCCTCTCCAGTCGCACCACCGTGGTGGCCATTGGCGCAGGGCTT CCTGCCGCCATCATCGGCGAACGGATCAACCCCACGGGCAAGAAGGGATTTGCCCAGGAACTGCAGGATGGAAAGGTCTC CTACATCCGGCGGGAGGCCCTGGAGCAGACCGCCCGCGGCGCCGTCCTTCTGGACGTTAACGTGGGTACGCCGGGAATCG ACGAACCGGCCGCCATGGAACGGGCCGTCGTTACCGTTGCCACTGCAGTTGGCGCGCCGCTGGTCCTTGATTCGTCCGAT CCCGCCGCCCTGGAGCGGGGGCTTAAGGCCGCCGACGGCAAGGTGCTCCTTAATTCCGTGAACGGCGAAGCAAAGAGCAT GGAGCGCGTTCTGCCCCTGGCACGGAAGTACGGGGCGGCCGTGATCGGACTTGCACTGGACGAGACGGGGATCCCAGAGA CCGCCGAGGGGCGTCTGGCCGTGGCGGAGCGGATCGTCAAGGCGGCCGGCAAGGCGGGTATCCGGCGGCCGGACGTGGTG ATCGATTGCCTCACCCTTACCGTGAGCGCCGAGCAGAAGCGGGCCATGGAAACCTTGAGAGCGGTGCGGCTCGTCAAGGA GCGGCTCGGCTGCCCCACGGTCCTCGGGGTCAGCAACATCTCCTTCGGCCTTCCCCGCCGGCCGCTCATCTCTTCGGCAT TCTTCGCCATGGCCCTGGCTGCCGGGCTCGATGCCGCCATCGTGAACCCGAAGGAAGAGGAGATGATGGCTGCCTGGCAT TCGTCAATGGTGCTTCTGAACCGGGATCCGAACGCCGCCGCCTACATCGCCGCCTATGCCGGCGTTGCTCCTCCCGCGGC CGAGCCGGCAGCGACGGGCGAGCTTCCCGATATCCGGCAGCGGCTCGCGCGGGCCGTAATAACCGGCGACGGAGAGGGAA TCGTCTCCCTGGTGGAGGAAGCCCTTTCCCATGGGCTTGAGCCGCTCCAGGTGAGCAACGAGGGACTCCTGCCGGGACTG GAGGAGGTGGGGCGCCGCTTCGAGAAAAACCAGGTGTTCCTCCCCCAGGTCATGCAGTCGGCCGAGGCCATGCAGGCCGC GTTCGCGCGGCTCAAGCAGGAGATGACAGGAGAGGCGGAATCGAAGGGGACCATCCTCATGGCCACGGTGGAAGGGGATA TCCACGATATCGGCAAGAACATCGTCTGCACTCTGCTGGAAAACCACGGGTTCCGGGTCATCGATCTGGGCAAGAACGTG CCGGCGGACCGGATCGTGGCGGAAGCCGAGCGCAACGGGGCCGATGCGGTGGGGCTCTCGGCCCTCATGACCACAACCAT GACCGAGATGGAAAACGTGCTCGCCCGCCTCAGGGCTGCCGGCATCCGGACTTTCACCATGGTGGGGGGAGCGGTGGTGA CCCAGGAGTATGCCGACCGGATCGGCGCAGACCTTTATGCCCGGGATGCCATGGACGCGGTGGCGCGTATAAAGGCGCTC CTCGGGGTGGATTAG
Upstream 100 bases:
>100_bases CCAGTGAGCGGGTGAGGGCAACGTCCGGTCGCCGGGCACTGGGGCTGCCCCGGGCGGCCGATCGAATCGATCCGTAACTA TTGCCAAAGGTTGGTGAACC
Downstream 100 bases:
>100_bases TCATTGCGTTACGCGGAGTTGCGTGCTAGTTTAACCTGTTGCGTCTCTTGATGGATGGACAGCCATGAACGTCGGATTCA ACCACAACGTCATGTACCGG
Product: 5-methyltetrahydrofolate-homocysteine methyltransferase, truncation
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 804; Mature: 804
Protein sequence:
>804_residues MKQPFLQAIAERVLVLDGAMGTMLQERGLRPGQSPEELNLTLPEVVAGVHREYLDAGADIIVTNTFGGSRAKLEHYGLQD RVAEINARAVAIAREVCGDRAYVAASIGPTGQFVEPVGDVSFDEMAAIFREQAQALINAGADLITLETFLDIKEIRAAVI AIREISPETPVIAQLTFDNEGRTVLGTSPEAAAVTLEAAGADIVGSNCGLGPDGICDVMAAMRRVTRLPLISQANAGLPT LVDGATVFPGTPDDMTAFHDRLLDLNVRVIGGCCGTTPAHIRAIREALALRDQSLRACGPAEGVTFLSSRTTVVAIGAGL PAAIIGERINPTGKKGFAQELQDGKVSYIRREALEQTARGAVLLDVNVGTPGIDEPAAMERAVVTVATAVGAPLVLDSSD PAALERGLKAADGKVLLNSVNGEAKSMERVLPLARKYGAAVIGLALDETGIPETAEGRLAVAERIVKAAGKAGIRRPDVV IDCLTLTVSAEQKRAMETLRAVRLVKERLGCPTVLGVSNISFGLPRRPLISSAFFAMALAAGLDAAIVNPKEEEMMAAWH SSMVLLNRDPNAAAYIAAYAGVAPPAAEPAATGELPDIRQRLARAVITGDGEGIVSLVEEALSHGLEPLQVSNEGLLPGL EEVGRRFEKNQVFLPQVMQSAEAMQAAFARLKQEMTGEAESKGTILMATVEGDIHDIGKNIVCTLLENHGFRVIDLGKNV PADRIVAEAERNGADAVGLSALMTTTMTEMENVLARLRAAGIRTFTMVGGAVVTQEYADRIGADLYARDAMDAVARIKAL LGVD
Sequences:
>Translated_804_residues MKQPFLQAIAERVLVLDGAMGTMLQERGLRPGQSPEELNLTLPEVVAGVHREYLDAGADIIVTNTFGGSRAKLEHYGLQD RVAEINARAVAIAREVCGDRAYVAASIGPTGQFVEPVGDVSFDEMAAIFREQAQALINAGADLITLETFLDIKEIRAAVI AIREISPETPVIAQLTFDNEGRTVLGTSPEAAAVTLEAAGADIVGSNCGLGPDGICDVMAAMRRVTRLPLISQANAGLPT LVDGATVFPGTPDDMTAFHDRLLDLNVRVIGGCCGTTPAHIRAIREALALRDQSLRACGPAEGVTFLSSRTTVVAIGAGL PAAIIGERINPTGKKGFAQELQDGKVSYIRREALEQTARGAVLLDVNVGTPGIDEPAAMERAVVTVATAVGAPLVLDSSD PAALERGLKAADGKVLLNSVNGEAKSMERVLPLARKYGAAVIGLALDETGIPETAEGRLAVAERIVKAAGKAGIRRPDVV IDCLTLTVSAEQKRAMETLRAVRLVKERLGCPTVLGVSNISFGLPRRPLISSAFFAMALAAGLDAAIVNPKEEEMMAAWH SSMVLLNRDPNAAAYIAAYAGVAPPAAEPAATGELPDIRQRLARAVITGDGEGIVSLVEEALSHGLEPLQVSNEGLLPGL EEVGRRFEKNQVFLPQVMQSAEAMQAAFARLKQEMTGEAESKGTILMATVEGDIHDIGKNIVCTLLENHGFRVIDLGKNV PADRIVAEAERNGADAVGLSALMTTTMTEMENVLARLRAAGIRTFTMVGGAVVTQEYADRIGADLYARDAMDAVARIKAL LGVD >Mature_804_residues MKQPFLQAIAERVLVLDGAMGTMLQERGLRPGQSPEELNLTLPEVVAGVHREYLDAGADIIVTNTFGGSRAKLEHYGLQD RVAEINARAVAIAREVCGDRAYVAASIGPTGQFVEPVGDVSFDEMAAIFREQAQALINAGADLITLETFLDIKEIRAAVI AIREISPETPVIAQLTFDNEGRTVLGTSPEAAAVTLEAAGADIVGSNCGLGPDGICDVMAAMRRVTRLPLISQANAGLPT LVDGATVFPGTPDDMTAFHDRLLDLNVRVIGGCCGTTPAHIRAIREALALRDQSLRACGPAEGVTFLSSRTTVVAIGAGL PAAIIGERINPTGKKGFAQELQDGKVSYIRREALEQTARGAVLLDVNVGTPGIDEPAAMERAVVTVATAVGAPLVLDSSD PAALERGLKAADGKVLLNSVNGEAKSMERVLPLARKYGAAVIGLALDETGIPETAEGRLAVAERIVKAAGKAGIRRPDVV IDCLTLTVSAEQKRAMETLRAVRLVKERLGCPTVLGVSNISFGLPRRPLISSAFFAMALAAGLDAAIVNPKEEEMMAAWH SSMVLLNRDPNAAAYIAAYAGVAPPAAEPAATGELPDIRQRLARAVITGDGEGIVSLVEEALSHGLEPLQVSNEGLLPGL EEVGRRFEKNQVFLPQVMQSAEAMQAAFARLKQEMTGEAESKGTILMATVEGDIHDIGKNIVCTLLENHGFRVIDLGKNV PADRIVAEAERNGADAVGLSALMTTTMTEMENVLARLRAAGIRTFTMVGGAVVTQEYADRIGADLYARDAMDAVARIKAL LGVD
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=848, Percent_Identity=31.1320754716981, Blast_Score=339, Evalue=6e-93, Organism=Homo sapiens, GI157266337, Length=301, Percent_Identity=31.5614617940199, Blast_Score=108, Evalue=3e-23, Organism=Homo sapiens, GI13162290, Length=298, Percent_Identity=31.2080536912752, Blast_Score=105, Evalue=1e-22, Organism=Escherichia coli, GI1790450, Length=868, Percent_Identity=31.221198156682, Blast_Score=328, Evalue=8e-91, Organism=Escherichia coli, GI1786456, Length=301, Percent_Identity=28.2392026578073, Blast_Score=81, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17535405, Length=847, Percent_Identity=31.6410861865407, Blast_Score=341, Evalue=9e-94, Organism=Saccharomyces cerevisiae, GI6324982, Length=326, Percent_Identity=25.4601226993865, Blast_Score=81, Evalue=7e-16, Organism=Saccharomyces cerevisiae, GI6322966, Length=320, Percent_Identity=25.625, Blast_Score=77, Evalue=1e-14, Organism=Drosophila melanogaster, GI24585077, Length=282, Percent_Identity=25.886524822695, Blast_Score=78, Evalue=3e-14,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 84671; Mature: 84671
Theoretical pI: Translated: 4.73; Mature: 4.73
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKQPFLQAIAERVLVLDGAMGTMLQERGLRPGQSPEELNLTLPEVVAGVHREYLDAGADI CCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCE IVTNTFGGSRAKLEHYGLQDRVAEINARAVAIAREVCGDRAYVAASIGPTGQFVEPVGDV EEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCC SFDEMAAIFREQAQALINAGADLITLETFLDIKEIRAAVIAIREISPETPVIAQLTFDNE CHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC GRTVLGTSPEAAAVTLEAAGADIVGSNCGLGPDGICDVMAAMRRVTRLPLISQANAGLPT CCEEECCCCCCEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE LVDGATVFPGTPDDMTAFHDRLLDLNVRVIGGCCGTTPAHIRAIREALALRDQSLRACGP EECCCEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC AEGVTFLSSRTTVVAIGAGLPAAIIGERINPTGKKGFAQELQDGKVSYIRREALEQTARG CCCCEEEECCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC AVLLDVNVGTPGIDEPAAMERAVVTVATAVGAPLVLDSSDPAALERGLKAADGKVLLNSV EEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCEEEEECC NGEAKSMERVLPLARKYGAAVIGLALDETGIPETAEGRLAVAERIVKAAGKAGIRRPDVV CCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE IDCLTLTVSAEQKRAMETLRAVRLVKERLGCPTVLGVSNISFGLPRRPLISSAFFAMALA EEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH AGLDAAIVNPKEEEMMAAWHSSMVLLNRDPNAAAYIAAYAGVAPPAAEPAATGELPDIRQ HCCCCEECCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHH RLARAVITGDGEGIVSLVEEALSHGLEPLQVSNEGLLPGLEEVGRRFEKNQVFLPQVMQS HHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCEEHHHHHHH AEAMQAAFARLKQEMTGEAESKGTILMATVEGDIHDIGKNIVCTLLENHGFRVIDLGKNV HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHCHHHHHHHHHCCCEEEEECCCCC PADRIVAEAERNGADAVGLSALMTTTMTEMENVLARLRAAGIRTFTMVGGAVVTQEYADR CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHCCHHHHHHHHHH IGADLYARDAMDAVARIKALLGVD HCCHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MKQPFLQAIAERVLVLDGAMGTMLQERGLRPGQSPEELNLTLPEVVAGVHREYLDAGADI CCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCE IVTNTFGGSRAKLEHYGLQDRVAEINARAVAIAREVCGDRAYVAASIGPTGQFVEPVGDV EEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCC SFDEMAAIFREQAQALINAGADLITLETFLDIKEIRAAVIAIREISPETPVIAQLTFDNE CHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC GRTVLGTSPEAAAVTLEAAGADIVGSNCGLGPDGICDVMAAMRRVTRLPLISQANAGLPT CCEEECCCCCCEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE LVDGATVFPGTPDDMTAFHDRLLDLNVRVIGGCCGTTPAHIRAIREALALRDQSLRACGP EECCCEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC AEGVTFLSSRTTVVAIGAGLPAAIIGERINPTGKKGFAQELQDGKVSYIRREALEQTARG CCCCEEEECCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC AVLLDVNVGTPGIDEPAAMERAVVTVATAVGAPLVLDSSDPAALERGLKAADGKVLLNSV EEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCEEEEECC NGEAKSMERVLPLARKYGAAVIGLALDETGIPETAEGRLAVAERIVKAAGKAGIRRPDVV CCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE IDCLTLTVSAEQKRAMETLRAVRLVKERLGCPTVLGVSNISFGLPRRPLISSAFFAMALA EEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH AGLDAAIVNPKEEEMMAAWHSSMVLLNRDPNAAAYIAAYAGVAPPAAEPAATGELPDIRQ HCCCCEECCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHH RLARAVITGDGEGIVSLVEEALSHGLEPLQVSNEGLLPGLEEVGRRFEKNQVFLPQVMQS HHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCEEHHHHHHH AEAMQAAFARLKQEMTGEAESKGTILMATVEGDIHDIGKNIVCTLLENHGFRVIDLGKNV HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHCHHHHHHHHHCCCEEEEECCCCC PADRIVAEAERNGADAVGLSALMTTTMTEMENVLARLRAAGIRTFTMVGGAVVTQEYADR CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHCCHHHHHHHHHH IGADLYARDAMDAVARIKALLGVD HCCHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9634230 [H]