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The map label for this gene is zraS [H]

Identifier: 39998007

GI number: 39998007

Start: 3215428

End: 3216891

Strand: Reverse

Name: zraS [H]

Synonym: GSU2916

Alternate gene names: 39998007

Gene position: 3216891-3215428 (Counterclockwise)

Preceding gene: 39998008

Following gene: 39998006

Centisome position: 84.34

GC content: 59.9

Gene sequence:

>1464_bases
GTGATAATCATCCTGGTCGGCCTGCTTTCCGTGGGGATCGGTACCTTTACCTATCTTAACTTCAAGCGGGAGAAGCGCCA
GCTGATCGCGGCGGCCCGCGAGAATACACTCCTGCTCCTCAATACCATTGAGCGGAGCATCTATCGTTCCATGAGCATCG
GCGATACCCAGGACGTCCAGCGCATGCTGGAGATGGTCGGCAAGAGCCACTCTAAGCTCCTGGGGGTGCGGATATTCCAT
CCCCAGGGGGTTGTCCTCAAGTCGTCGCTCCCCTCTGAGATGGGCGGAACGGTGCCGGACGGAGACTACCAGCTCTTTGC
CAACAATCTGCGGGAAGGGATCTTCGCCAGCGGCAGCCACGGTGAAGTCTTCGGCATGATCAAGCCGATCTACAACGAGG
AGCAGTGCCACATCTGCCACGGTCGCAAGCGGCGGGTCATCGGAGTGCTCAACGTCAACTACTCCCTGGCGGATACCAAT
CGGCGCATCAACGAGGCGACCCGTCTCTTCCTCTTCTCCACCGGCGCCATCATCCTTTTTAGCGCGGCTGCTGTTTCTCT
CGTCATGGTGCGTTTCGTGAGGCAGCCGCTCAATCGTATCGTGGAGAGCATGGCCCGGGTGGAGCAGGGCGATCTGAAGG
TCAGGATGGTCAACGGCGGCCGGGATGAGATCGGTCGGCTCATAACCAGTTTTGACTCCATGGTCGACAAGCTCGACAAG
GCCAAGCAAGAGCTGGAGCAGTATCACTTCCAGCAGATGGAGCGGGCCGACCGGCTTGCGTCCGTGGGAGAAATGGCCGC
TGGTATCGCCCATGAGATCAAGAACCCGCTGGCCGGGATATCCGCAGCAATGACCATCATCCGCCAGGATTTCCAGGAGG
AGGATCCCCGCCGGGAAATCATCGGCGAGGTAATCGAGCAGGTCAACCGTCTGGACAAGACGGTCAACGATCTTCTCTTC
TTCGGCAAGCCGACCCATCCGGAGCCCGCTCCCACCGATCTGAACGATGTCTTGCGCAAGACCCTGCTCTTTGCCCTTCA
GCACAAAGGCGGGAAGAACATCGAGAAACGGTTGGAGTTGCAGGAGCCGCTCCCCCTCGTTTTCGTGGACCCCAAGCAGG
TGCAGCAGGTGGTGCTCAACCTGATCCTGAACGCCATCCAGGCCATGCAGGAGACCGGTGGCACCCTCACCGTTGCCAGC
CGTATCGCGGAGCGCGAGGGAGGTTTGTGGGTCCGCGTGGAGGTGGCCGATACGGGGCCGGGTATTCCCGAGCCGATCAT
CAGCAAAATCTTCACTCCGTTTTTCACCACCAAGGCCCAGGGGACGGGGCTTGGCCTGGCCATTTGCCAGAAGCTCATCA
CGCAGCACGGCGGAACCCTCCATGTCTCGAGTGTGCTTAACCAGGGCACGGTTTTTTCCATCGACCTCCCCATGACCGAA
CTGGAGGCCGACGGTGCCGTATGA

Upstream 100 bases:

>100_bases
ACGCTGATGTTTTTCTCGCTACTTACATTACCCGGCGGCTGTTCTTCGTGAACAGCCGGCCCATGGGGGCAGGTTGGTAT
TCAGAAGCCTCACAACACGT

Downstream 100 bases:

>100_bases
GGAATGGCCGGCGCGGGAAGAGCGCTGTGTGCATCAAAGGATTTCCCTATCTGCTGCCGGTAATGCTATAGTGGGTTCAT
TATACGATTCAATGCGTTAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MIIILVGLLSVGIGTFTYLNFKREKRQLIAAARENTLLLLNTIERSIYRSMSIGDTQDVQRMLEMVGKSHSKLLGVRIFH
PQGVVLKSSLPSEMGGTVPDGDYQLFANNLREGIFASGSHGEVFGMIKPIYNEEQCHICHGRKRRVIGVLNVNYSLADTN
RRINEATRLFLFSTGAIILFSAAAVSLVMVRFVRQPLNRIVESMARVEQGDLKVRMVNGGRDEIGRLITSFDSMVDKLDK
AKQELEQYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFQEEDPRREIIGEVIEQVNRLDKTVNDLLF
FGKPTHPEPAPTDLNDVLRKTLLFALQHKGGKNIEKRLELQEPLPLVFVDPKQVQQVVLNLILNAIQAMQETGGTLTVAS
RIAEREGGLWVRVEVADTGPGIPEPIISKIFTPFFTTKAQGTGLGLAICQKLITQHGGTLHVSSVLNQGTVFSIDLPMTE
LEADGAV

Sequences:

>Translated_487_residues
MIIILVGLLSVGIGTFTYLNFKREKRQLIAAARENTLLLLNTIERSIYRSMSIGDTQDVQRMLEMVGKSHSKLLGVRIFH
PQGVVLKSSLPSEMGGTVPDGDYQLFANNLREGIFASGSHGEVFGMIKPIYNEEQCHICHGRKRRVIGVLNVNYSLADTN
RRINEATRLFLFSTGAIILFSAAAVSLVMVRFVRQPLNRIVESMARVEQGDLKVRMVNGGRDEIGRLITSFDSMVDKLDK
AKQELEQYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFQEEDPRREIIGEVIEQVNRLDKTVNDLLF
FGKPTHPEPAPTDLNDVLRKTLLFALQHKGGKNIEKRLELQEPLPLVFVDPKQVQQVVLNLILNAIQAMQETGGTLTVAS
RIAEREGGLWVRVEVADTGPGIPEPIISKIFTPFFTTKAQGTGLGLAICQKLITQHGGTLHVSSVLNQGTVFSIDLPMTE
LEADGAV
>Mature_487_residues
MIIILVGLLSVGIGTFTYLNFKREKRQLIAAARENTLLLLNTIERSIYRSMSIGDTQDVQRMLEMVGKSHSKLLGVRIFH
PQGVVLKSSLPSEMGGTVPDGDYQLFANNLREGIFASGSHGEVFGMIKPIYNEEQCHICHGRKRRVIGVLNVNYSLADTN
RRINEATRLFLFSTGAIILFSAAAVSLVMVRFVRQPLNRIVESMARVEQGDLKVRMVNGGRDEIGRLITSFDSMVDKLDK
AKQELEQYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFQEEDPRREIIGEVIEQVNRLDKTVNDLLF
FGKPTHPEPAPTDLNDVLRKTLLFALQHKGGKNIEKRLELQEPLPLVFVDPKQVQQVVLNLILNAIQAMQETGGTLTVAS
RIAEREGGLWVRVEVADTGPGIPEPIISKIFTPFFTTKAQGTGLGLAICQKLITQHGGTLHVSSVLNQGTVFSIDLPMTE
LEADGAV

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=37.6623376623377, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=34.061135371179, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1790300, Length=236, Percent_Identity=32.6271186440678, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789149, Length=341, Percent_Identity=27.5659824046921, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87081816, Length=320, Percent_Identity=29.0625, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788393, Length=300, Percent_Identity=26.3333333333333, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693157, Length=260, Percent_Identity=28.4615384615385, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786783, Length=342, Percent_Identity=23.3918128654971, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI48994928, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790861, Length=345, Percent_Identity=25.7971014492754, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786912, Length=285, Percent_Identity=27.719298245614, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=25.3275109170306, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789808, Length=320, Percent_Identity=24.6875, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53941; Mature: 53941

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIILVGLLSVGIGTFTYLNFKREKRQLIAAARENTLLLLNTIERSIYRSMSIGDTQDVQ
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHH
RMLEMVGKSHSKLLGVRIFHPQGVVLKSSLPSEMGGTVPDGDYQLFANNLREGIFASGSH
HHHHHHCCCCHHEEEEEEECCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
GEVFGMIKPIYNEEQCHICHGRKRRVIGVLNVNYSLADTNRRINEATRLFLFSTGAIILF
CCEEEECCCCCCCCCEEEECCCCCEEEEEEECCEEECCCHHHHHHHHEEHHEECCHHHHH
SAAAVSLVMVRFVRQPLNRIVESMARVEQGDLKVRMVNGGRDEIGRLITSFDSMVDKLDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
AKQELEQYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFQEEDPRREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
IGEVIEQVNRLDKTVNDLLFFGKPTHPEPAPTDLNDVLRKTLLFALQHKGGKNIEKRLEL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
QEPLPLVFVDPKQVQQVVLNLILNAIQAMQETGGTLTVASRIAEREGGLWVRVEVADTGP
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCEEEEEEEECCCC
GIPEPIISKIFTPFFTTKAQGTGLGLAICQKLITQHGGTLHVSSVLNQGTVFSIDLPMTE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCCEEEEECCHHH
LEADGAV
CCCCCCC
>Mature Secondary Structure
MIIILVGLLSVGIGTFTYLNFKREKRQLIAAARENTLLLLNTIERSIYRSMSIGDTQDVQ
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHH
RMLEMVGKSHSKLLGVRIFHPQGVVLKSSLPSEMGGTVPDGDYQLFANNLREGIFASGSH
HHHHHHCCCCHHEEEEEEECCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
GEVFGMIKPIYNEEQCHICHGRKRRVIGVLNVNYSLADTNRRINEATRLFLFSTGAIILF
CCEEEECCCCCCCCCEEEECCCCCEEEEEEECCEEECCCHHHHHHHHEEHHEECCHHHHH
SAAAVSLVMVRFVRQPLNRIVESMARVEQGDLKVRMVNGGRDEIGRLITSFDSMVDKLDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
AKQELEQYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFQEEDPRREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
IGEVIEQVNRLDKTVNDLLFFGKPTHPEPAPTDLNDVLRKTLLFALQHKGGKNIEKRLEL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
QEPLPLVFVDPKQVQQVVLNLILNAIQAMQETGGTLTVASRIAEREGGLWVRVEVADTGP
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCEEEEEEEECCCC
GIPEPIISKIFTPFFTTKAQGTGLGLAICQKLITQHGGTLHVSSVLNQGTVFSIDLPMTE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCCEEEEECCHHH
LEADGAV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]