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The map label for this gene is truA

Identifier: 39997968

GI number: 39997968

Start: 3152554

End: 3153288

Strand: Reverse

Name: truA

Synonym: GSU2877

Alternate gene names: 39997968

Gene position: 3153288-3152554 (Counterclockwise)

Preceding gene: 39997969

Following gene: 39997967

Centisome position: 82.67

GC content: 66.12

Gene sequence:

>735_bases
ATGCGGACCATCAAACTCATACTCGAATATGACGGCACCAACTATGCCGGCTGGCAGCTGCAGCCCAACGGTCTTTCCAT
TCAGGAGGTGGTGGAGGGCGCCTTGGCGCGCCTGCTCAAGGAGCCCGTGCGGTTGCGTGCATCGGGACGCACCGATGCCG
GGGTCCATGCCCGGGGGATGGTGGCTGCCTTTGACACGGACCGGTCGATTCCCCTGCGCGCGTTTTCGGATGGTCTCAAT
GCCCTGCTTCCGCCGGACATCGCCGTGCGAAGCGCCGATGAGGCCCTGCCGGGCTTCAACCCGCGGTTTGCGGCCACCGG
CAAGCATTATCGGTACACCATCCATCGGGGAGAGCGCCGCTCCCCCTTGGTCCGCCTCCAGTCATGGCACGTGCGGGGGG
CGCTCAACCTCGCCGCCATGCGCGAAGCGGCCCGTCACCTGACAGGTGAGCGGGATTTCGCCTCCTTCCGCACTGCCGGC
TGTGCCGCACGGACCACCATTCGCCGGGTGGATGCCGTGGAGATCAGTGACGACGGGGAGATGCTTACGGTGGATGTCCA
TGGCTCGGGCTTTCTGCGCAACATGGTCCGGATCATGGTGGGCACGCTGGTGGAGGTGGGGCGGGGGAAGCTGACGCCGG
AGCACGTGGCGCAGATGGTTGTCTGCCCGGGCGTCGTTCCGGCCGGTCCCACCGCTCCTCCCCAGGGACTCTGTCTCCAA
AAGGTCCGTTTCTGA

Upstream 100 bases:

>100_bases
CCGGATGCTCCGCATCCTGGTGGAAAAGTAGCGGCTCAGCCGCGAACGTACCGTCACGCAGTACCAGGACGCCCCGCCAG
CGGGGCGTCTTCACGTTTCC

Downstream 100 bases:

>100_bases
GGAAAAGTTTTTGCTTGACAGCGTGTTGAGCCCATAGTACCATCCGTCCTTCGCTGAGAAAAGCTCGGTTTACACATTCA
TTCCATCCATGCTGTGAGGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRTIKLILEYDGTNYAGWQLQPNGLSIQEVVEGALARLLKEPVRLRASGRTDAGVHARGMVAAFDTDRSIPLRAFSDGLN
ALLPPDIAVRSADEALPGFNPRFAATGKHYRYTIHRGERRSPLVRLQSWHVRGALNLAAMREAARHLTGERDFASFRTAG
CAARTTIRRVDAVEISDDGEMLTVDVHGSGFLRNMVRIMVGTLVEVGRGKLTPEHVAQMVVCPGVVPAGPTAPPQGLCLQ
KVRF

Sequences:

>Translated_244_residues
MRTIKLILEYDGTNYAGWQLQPNGLSIQEVVEGALARLLKEPVRLRASGRTDAGVHARGMVAAFDTDRSIPLRAFSDGLN
ALLPPDIAVRSADEALPGFNPRFAATGKHYRYTIHRGERRSPLVRLQSWHVRGALNLAAMREAARHLTGERDFASFRTAG
CAARTTIRRVDAVEISDDGEMLTVDVHGSGFLRNMVRIMVGTLVEVGRGKLTPEHVAQMVVCPGVVPAGPTAPPQGLCLQ
KVRF
>Mature_244_residues
MRTIKLILEYDGTNYAGWQLQPNGLSIQEVVEGALARLLKEPVRLRASGRTDAGVHARGMVAAFDTDRSIPLRAFSDGLN
ALLPPDIAVRSADEALPGFNPRFAATGKHYRYTIHRGERRSPLVRLQSWHVRGALNLAAMREAARHLTGERDFASFRTAG
CAARTTIRRVDAVEISDDGEMLTVDVHGSGFLRNMVRIMVGTLVEVGRGKLTPEHVAQMVVCPGVVPAGPTAPPQGLCLQ
KVRF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=30.4832713754647, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=37.7593360995851, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321112, Length=240, Percent_Identity=27.9166666666667, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_GEOSL (P60350)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953919.1
- ProteinModelPortal:   P60350
- SMR:   P60350
- GeneID:   2688667
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2877
- NMPDR:   fig|243231.1.peg.2857
- TIGR:   GSU2877
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   GSUL243231:GSU_2877-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 26642; Mature: 26642

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIKLILEYDGTNYAGWQLQPNGLSIQEVVEGALARLLKEPVRLRASGRTDAGVHARGM
CCEEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCE
VAAFDTDRSIPLRAFSDGLNALLPPDIAVRSADEALPGFNPRFAATGKHYRYTIHRGERR
EEEECCCCCCCHHHHHCCCCCCCCCCCEECCCHHHCCCCCCCEEECCCEEEEEEECCCCC
SPLVRLQSWHVRGALNLAAMREAARHLTGERDFASFRTAGCAARTTIRRVDAVEISDDGE
CCEEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCEEEEECCCCC
MLTVDVHGSGFLRNMVRIMVGTLVEVGRGKLTPEHVAQMVVCPGVVPAGPTAPPQGLCLQ
EEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KVRF
HCCC
>Mature Secondary Structure
MRTIKLILEYDGTNYAGWQLQPNGLSIQEVVEGALARLLKEPVRLRASGRTDAGVHARGM
CCEEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCE
VAAFDTDRSIPLRAFSDGLNALLPPDIAVRSADEALPGFNPRFAATGKHYRYTIHRGERR
EEEECCCCCCCHHHHHCCCCCCCCCCCEECCCHHHCCCCCCCEEECCCEEEEEEECCCCC
SPLVRLQSWHVRGALNLAAMREAARHLTGERDFASFRTAGCAARTTIRRVDAVEISDDGE
CCEEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCEEEEECCCCC
MLTVDVHGSGFLRNMVRIMVGTLVEVGRGKLTPEHVAQMVVCPGVVPAGPTAPPQGLCLQ
EEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KVRF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA