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The map label for this gene is tuf-1 [H]

Identifier: 39997951

GI number: 39997951

Start: 3129545

End: 3130735

Strand: Reverse

Name: tuf-1 [H]

Synonym: GSU2859

Alternate gene names: 39997951

Gene position: 3130735-3129545 (Counterclockwise)

Preceding gene: 39997952

Following gene: 39997950

Centisome position: 82.08

GC content: 60.54

Gene sequence:

>1191_bases
ATGGCAAAGGCAAAATTCGAGAGGACGAAACCGCACGTCAACATAGGAACGATCGGCCACGTAGACCATGGCAAGACCAC
GCTGACGGCGGCCATAACCAAGGTATTGGCAGAGCGCGGCCAGGCAGAGTTCCGTGGTTTTGACCAGATTGACAACGCTC
CGGAAGAGCGCGAGCGCGGTATCACCATCGCCACGTCCCATGTGGAGTACGAGACCGAGAAGCGTCACTACGCCCACGTG
GACTGCCCGGGTCACGCCGACTATGTAAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCGATCCTCGTCGTATC
CGCCGCCGATGGCCCCATGCCGCAGACCCGCGAGCACATCCTGCTTGCCCGTCAGGTTGGCGTGCCCTACATTGTCGTGT
TCCTGAACAAGGCCGACATGGTAGACGACGAAGAGCTGCTCGAGCTGGTAGAGCTCGAGATTCGCGAGCTGCTCTCCTCC
TACGACTTCCCGGGTGACGACATCCCGATCATCAAGGGGAGCGCGCTCAAGGGGCTCAATGGCGACAAGGACGAGCTGGG
CGAAGAAGCCATCCTGAAGCTGATGGAAGCCGTTGACAACTATATTCCCGAGCCCGAGCGGGCCGTAGACAAGCCGTTCC
TGATGCCTGTGGAGGACGTATTCTCGATCTCCGGTCGCGGTACCGTAGCCACGGGGCGTGTGGAGCGCGGAATTGTCAAG
GTGGGTGAGGAGGTCGAGATCGTCGGGATCAAGGCCACTGCCAAGACCACGGTGACCGGAGTCGAGATGTTCCGCAAGCT
TCTGGACGAAGGCCGCGCCGGTGACAACATCGGTGCCCTTCTGCGGGGGGTGAAGCGTGAAGACATCGAGCGTGGCCAGG
TGCTTGCGAAGCCGGGGAGCATCACTCCGCACACCAAGTTCAAGGCCGAGGCGTACATCCTGACGAAGGAAGAAGGTGGC
CGTCACACGCCGTTCTTCAACGGGTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACTGGCGTAGTAGACCTTCC
TGCCGGGACTGAGATGGTAATGCCTGGCGACAACGTGGCGGTGACGATCAACCTGATCACGCCGATCGCGATGGATGAAG
GTCTTCGGTTCGCGATTCGCGAAGGTGGCCGTACTGTGGGCGCCGGCGTCGTCAGCTCCATTATCGAATAA

Upstream 100 bases:

>100_bases
GATGACATTCGATCATTACGAGCAGGTGCCTAAGTCGGTATCCGAAGAAATCGTCGCAAAGGTTAAAGGCTAGTCAAATT
CAGCGAGGGAGGAATCCTGC

Downstream 100 bases:

>100_bases
CGTTGTTGCGAGGAAGAGATGCCAAGTCAAAAGATTAGAATCCGCCTTAAGGCATTTGATCATAAACTCCTCGACCAATC
GGTCGGAGAGATTGTTGACA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAERGQAEFRGFDQIDNAPEERERGITIATSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSS
YDFPGDDIPIIKGSALKGLNGDKDELGEEAILKLMEAVDNYIPEPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSITPHTKFKAEAYILTKEEGG
RHTPFFNGYRPQFYFRTTDVTGVVDLPAGTEMVMPGDNVAVTINLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAERGQAEFRGFDQIDNAPEERERGITIATSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSS
YDFPGDDIPIIKGSALKGLNGDKDELGEEAILKLMEAVDNYIPEPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSITPHTKFKAEAYILTKEEGG
RHTPFFNGYRPQFYFRTTDVTGVVDLPAGTEMVMPGDNVAVTINLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAERGQAEFRGFDQIDNAPEERERGITIATSHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSY
DFPGDDIPIIKGSALKGLNGDKDELGEEAILKLMEAVDNYIPEPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVKV
GEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSITPHTKFKAEAYILTKEEGGR
HTPFFNGYRPQFYFRTTDVTGVVDLPAGTEMVMPGDNVAVTINLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=492, Evalue=1e-139,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=30.9954751131222, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=31.1512415349887, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=31.2359550561798, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=31.2359550561798, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI194018522, Length=433, Percent_Identity=29.0993071593533, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI194097354, Length=433, Percent_Identity=29.0993071593533, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI194018520, Length=433, Percent_Identity=29.0993071593533, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI46094014, Length=434, Percent_Identity=28.8018433179723, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=28.4057971014493, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4503507, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI82546879, Length=440, Percent_Identity=24.5454545454545, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=78.9873417721519, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=78.9873417721519, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.8941798941799, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=33.7301587301587, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789108, Length=438, Percent_Identity=28.0821917808219, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=33.1034482758621, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=31.5068493150685, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.1328320802005, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.702842377261, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=30.561797752809, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=30.561797752809, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=30, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=27.1111111111111, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=28.2110091743119, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.2110091743119, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.4314381270903, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=40, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=275, Percent_Identity=28, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=67.0854271356784, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=30.0225733634311, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=30.0225733634311, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=25.0549450549451, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=30.2052785923754, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=26.978417266187, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=31.6872427983539, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=33.7078651685393, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.5419847328244, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.5419847328244, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=49.6103896103896, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=30.4054054054054, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=30.4054054054054, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=30.4054054054054, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=30.4054054054054, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=32.1311475409836, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.2935779816514, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=31.4540059347181, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=28.8256227758007, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=28.8256227758007, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=36.3095238095238, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=35.1351351351351, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574573, Length=149, Percent_Identity=34.2281879194631, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43219; Mature: 43088

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAERGQAEFRGFDQIDNAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCHHHCCCHHHHHCC
ITIATSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKGLN
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GDKDELGEEAILKLMEAVDNYIPEPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHCCCCCEEEECHHHHHHHH
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGS
CCCEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCC
ITPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVDLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEE
VTINLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAERGQAEFRGFDQIDNAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCHHHCCCHHHHHCC
ITIATSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKGLN
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GDKDELGEEAILKLMEAVDNYIPEPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHCCCCCEEEECHHHHHHHH
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGS
CCCEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCC
ITPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVDLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEE
VTINLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA