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The map label for this gene is fusA-3 [H]

Identifier: 39997952

GI number: 39997952

Start: 3130763

End: 3132841

Strand: Reverse

Name: fusA-3 [H]

Synonym: GSU2860

Alternate gene names: 39997952

Gene position: 3132841-3130763 (Counterclockwise)

Preceding gene: 39997953

Following gene: 39997951

Centisome position: 82.14

GC content: 54.59

Gene sequence:

>2079_bases
GTGGCACGTCAGGTTTCGCTTGAAAAAACCCGTAACATCGGGATTATGGCTCATATTGACGCCGGCAAGACGACGACAAC
TGAGCGCATACTCTATTATACCGGTGTGACGCATAAGATTGGTGAGGTTCACGAAGGGGCCGCCACCATGGACTGGATGG
AGCAGGAGCAGGAGCGGGGGATTACGATAACCTCGGCAGCTACGACCTGTTTCTGGGGCGACCATCGGGTCAATATCATT
GATACTCCTGGTCACGTGGATTTCACTATCGAGGTCGAGCGTTCCCTGCGTGTGCTTGATGGGGCTGTTGCTGTATTCTG
TTCCGTTGGCGGTGTTGAGCCGCAGTCGGAAACAGTCTGGCGCCAGGCCGACAAGTATCGCGTTCCTCGCATTGCCTTTA
TTAATAAGATGGATCGAGTCGGTGCCGACTTTTTTCGCGGCGTCGGCATGATTCGTGATCGCCTCAAGGCTAATCCTGTG
CCGATTCAGCTGCCGATCGGGGCCGAAGATACCTATCGCGGGGTTGTTGACCTCGTCGAGATGAAGGCGATTATTTGGGA
CGAGGAGTCCCTGGGCGCCAAGTATCATGAGGCGGAGATTCCTGCTGATCTGGCAGAGATGGCGCAAGAGTACCGTGAGA
AGCTCATAGAAGAAATTGCTACGTTTGATGATGCGCTGATGGAGAAGTATCTCGGCGGTGAAGAGCTCACTACTGACGAA
ATCAAGGCTGCCGTTCGCAAGGCAACGATCGATATACAAATCTGTCCGGTCATCTGTGGTTCTGCATTCAAGAACAAGGG
GGTTCAGAACCTTCTTGACTCAGTGGTCGCATATCTTCCTTCGCCGCTGGACATTCCCGCAATTACCGGTATCGATGCCA
AGTCCGGCGAGGAAATAACCCGCAAGGCGTCCGATGACGAGCCGTTCTCGGCTCTTGCCTTCAAGATCATGACAGACCCC
TTTGTGGGTCAGCTCTGCTTCTTCCGTGTATATTCCGGCGTGCTCAACTCCGGTTCCTACGTGTATAACTCCACGAAAGA
AAAGAAAGAGCGTATTGGCCGCCTGCTGAAAATGCACGCCAATAAGCGTGAAGAAATCAAAGAGGTCTATGCTGGCGACA
TCGCGGCGGCGGTTGGCCTCAAGTATACGACGACCGGCGATACGCTTTGCCCTGAAGATTCCCCGGTAGTGCTCGAGTCT
ATTGAGTTCCCCGAGCCGGTTATCGCTATTGCCATTGAGCCCAAGACGAAGGCCGATCAGGAGAAGCTCGGCATCAGTCT
TCAGAAGCTTGCGAGTGAAGATCCTTCGTTCCGTGTCAGAACGGATGAGGAGACGGGTCAGACCATCATTTCGGGCATGG
GTGAACTGCACCTCGAGATTATCGTTGACCGCCTCATGCGCGAATTCAAGGTTGAGGCAAACGTTGGTAAGCCGCAGGTT
GCCTATCGCGAGACTGTTACCAAAAAGGTTAAAGTCGAAGGCAAATTCGTCCGGCAGTCCGGTGGTCGTGGTCAGTATGG
CCATGTCTGGATCGAGCTTGAGCCTCAAGAGGCTGGCAAGGGCTACGAGTTTGTGGACGCGATCAAGGGTGGTGTTGTTC
CCAGGGAGTATATCCCGGCAGTTGACAAGGGGATTCAGGAGGCGATGGAAACCGGGGTTCTCGCTGGTTATCCGACGGTT
GATTTCAAGGTCGCCCTCGTTGACGGATCGTACCACGAGGTTGACTCCTCAGAAATGGCATTTAAAATTGCCGGTTCCAT
GGCCTTCAAAGAGGCGGCGGCTAAAGCAGGCCCCGTGCTGCTCGAGCCGATCATGTCGGTGGAGGTCGTTGTTCCGGAAG
AATACATGGGCGATGTGATCGGCGACCTGAACTCCCGCCGTGGCAGAATCATGGGGATGGAGGGGCGTGCCGGCGCTCAG
GTTGTCAGCGCAATGGTTCCGCTGGCTCAGATGTTCGGTTACGCCACGGACCTCCGTTCCGCCACACAGGGCCGGGCAAC
CTACACGATGACATTCGATCATTACGAGCAGGTGCCTAAGTCGGTATCCGAAGAAATCGTCGCAAAGGTTAAAGGCTAG

Upstream 100 bases:

>100_bases
GCGAGGATACGCATCGTATGGCTGAGGCTAACAGGGCTTTTGCGCATTATCGCTGGTAGTAGTAAGTAGCTGGTCTTTGA
AACGCTAACTGGAGGGATTC

Downstream 100 bases:

>100_bases
TCAAATTCAGCGAGGGAGGAATCCTGCATGGCAAAGGCAAAATTCGAGAGGACGAAACCGCACGTCAACATAGGAACGAT
CGGCCACGTAGACCATGGCA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 1 [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MARQVSLEKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNII
DTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPV
PIQLPIGAEDTYRGVVDLVEMKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDE
IKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPLDIPAITGIDAKSGEEITRKASDDEPFSALAFKIMTDP
FVGQLCFFRVYSGVLNSGSYVYNSTKEKKERIGRLLKMHANKREEIKEVYAGDIAAAVGLKYTTTGDTLCPEDSPVVLES
IEFPEPVIAIAIEPKTKADQEKLGISLQKLASEDPSFRVRTDEETGQTIISGMGELHLEIIVDRLMREFKVEANVGKPQV
AYRETVTKKVKVEGKFVRQSGGRGQYGHVWIELEPQEAGKGYEFVDAIKGGVVPREYIPAVDKGIQEAMETGVLAGYPTV
DFKVALVDGSYHEVDSSEMAFKIAGSMAFKEAAAKAGPVLLEPIMSVEVVVPEEYMGDVIGDLNSRRGRIMGMEGRAGAQ
VVSAMVPLAQMFGYATDLRSATQGRATYTMTFDHYEQVPKSVSEEIVAKVKG

Sequences:

>Translated_692_residues
MARQVSLEKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNII
DTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPV
PIQLPIGAEDTYRGVVDLVEMKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDE
IKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPLDIPAITGIDAKSGEEITRKASDDEPFSALAFKIMTDP
FVGQLCFFRVYSGVLNSGSYVYNSTKEKKERIGRLLKMHANKREEIKEVYAGDIAAAVGLKYTTTGDTLCPEDSPVVLES
IEFPEPVIAIAIEPKTKADQEKLGISLQKLASEDPSFRVRTDEETGQTIISGMGELHLEIIVDRLMREFKVEANVGKPQV
AYRETVTKKVKVEGKFVRQSGGRGQYGHVWIELEPQEAGKGYEFVDAIKGGVVPREYIPAVDKGIQEAMETGVLAGYPTV
DFKVALVDGSYHEVDSSEMAFKIAGSMAFKEAAAKAGPVLLEPIMSVEVVVPEEYMGDVIGDLNSRRGRIMGMEGRAGAQ
VVSAMVPLAQMFGYATDLRSATQGRATYTMTFDHYEQVPKSVSEEIVAKVKG
>Mature_691_residues
ARQVSLEKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNIID
TPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVP
IQLPIGAEDTYRGVVDLVEMKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDEI
KAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPLDIPAITGIDAKSGEEITRKASDDEPFSALAFKIMTDPF
VGQLCFFRVYSGVLNSGSYVYNSTKEKKERIGRLLKMHANKREEIKEVYAGDIAAAVGLKYTTTGDTLCPEDSPVVLESI
EFPEPVIAIAIEPKTKADQEKLGISLQKLASEDPSFRVRTDEETGQTIISGMGELHLEIIVDRLMREFKVEANVGKPQVA
YRETVTKKVKVEGKFVRQSGGRGQYGHVWIELEPQEAGKGYEFVDAIKGGVVPREYIPAVDKGIQEAMETGVLAGYPTVD
FKVALVDGSYHEVDSSEMAFKIAGSMAFKEAAAKAGPVLLEPIMSVEVVVPEEYMGDVIGDLNSRRGRIMGMEGRAGAQV
VSAMVPLAQMFGYATDLRSATQGRATYTMTFDHYEQVPKSVSEEIVAKVKG

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=694, Percent_Identity=46.3976945244957, Blast_Score=590, Evalue=1e-168,
Organism=Homo sapiens, GI19923640, Length=710, Percent_Identity=41.5492957746479, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI25306283, Length=449, Percent_Identity=44.7661469933185, Blast_Score=345, Evalue=9e-95,
Organism=Homo sapiens, GI25306287, Length=285, Percent_Identity=52.9824561403509, Blast_Score=285, Evalue=1e-76,
Organism=Homo sapiens, GI217272894, Length=794, Percent_Identity=23.551637279597, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI217272892, Length=794, Percent_Identity=23.551637279597, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI4503483, Length=422, Percent_Identity=27.2511848341232, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=35.8108108108108, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI157426893, Length=141, Percent_Identity=35.4609929078014, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=36.2903225806452, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=36.2903225806452, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=36.2903225806452, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1789738, Length=699, Percent_Identity=66.8097281831188, Blast_Score=954, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=492, Percent_Identity=27.4390243902439, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=43.3823529411765, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788922, Length=142, Percent_Identity=36.6197183098592, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=680, Percent_Identity=42.3529411764706, Blast_Score=528, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17556745, Length=716, Percent_Identity=30.5865921787709, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17506493, Length=418, Percent_Identity=28.4688995215311, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=153, Percent_Identity=36.6013071895425, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=34.0740740740741, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=34.0740740740741, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=693, Percent_Identity=42.7128427128427, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322359, Length=783, Percent_Identity=32.6947637292465, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324707, Length=500, Percent_Identity=25.8, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=500, Percent_Identity=25.8, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323320, Length=161, Percent_Identity=34.7826086956522, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=35.6164383561644, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582462, Length=700, Percent_Identity=45, Blast_Score=584, Evalue=1e-167,
Organism=Drosophila melanogaster, GI221458488, Length=721, Percent_Identity=34.2579750346741, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24585709, Length=415, Percent_Identity=28.1927710843373, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585711, Length=415, Percent_Identity=28.1927710843373, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585713, Length=415, Percent_Identity=28.1927710843373, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI78706572, Length=142, Percent_Identity=35.9154929577465, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=35.6643356643357, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76202; Mature: 76071

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQVSLEKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERG
CCCCCCHHHHCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCC
ITITSAATTCFWGDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVW
EEEEECCEEEEECCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHH
RQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVPIQLPIGAEDTYRGVVDLVE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
MKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDE
HHHHEECCHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPLDIPAITGIDAKSGEEIT
HHHHHHHHEEEEEEHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
RKASDDEPFSALAFKIMTDPFVGQLCFFRVYSGVLNSGSYVYNSTKEKKERIGRLLKMHA
HCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHC
NKREEIKEVYAGDIAAAVGLKYTTTGDTLCPEDSPVVLESIEFPEPVIAIAIEPKTKADQ
CHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCH
EKLGISLQKLASEDPSFRVRTDEETGQTIISGMGELHLEIIVDRLMREFKVEANVGKPQV
HHHCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AYRETVTKKVKVEGKFVRQSGGRGQYGHVWIELEPQEAGKGYEFVDAIKGGVVPREYIPA
HHHHHHHHHEEECEEEEECCCCCCCCCEEEEEEEHHHCCCCCHHHHHHHCCCCCHHHHHH
VDKGIQEAMETGVLAGYPTVDFKVALVDGSYHEVDSSEMAFKIAGSMAFKEAAAKAGPVL
HHHHHHHHHHCCCEECCCCCEEEEEEECCCCCCCCCCHHEEEHHHHHHHHHHHHHCCCHH
LEPIMSVEVVVPEEYMGDVIGDLNSRRGRIMGMEGRAGAQVVSAMVPLAQMFGYATDLRS
HHCCCEEEEEECHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATQGRATYTMTFDHYEQVPKSVSEEIVAKVKG
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARQVSLEKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERG
CCCCCHHHHCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCC
ITITSAATTCFWGDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVW
EEEEECCEEEEECCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHH
RQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVPIQLPIGAEDTYRGVVDLVE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
MKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDE
HHHHEECCHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPLDIPAITGIDAKSGEEIT
HHHHHHHHEEEEEEHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
RKASDDEPFSALAFKIMTDPFVGQLCFFRVYSGVLNSGSYVYNSTKEKKERIGRLLKMHA
HCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHC
NKREEIKEVYAGDIAAAVGLKYTTTGDTLCPEDSPVVLESIEFPEPVIAIAIEPKTKADQ
CHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCH
EKLGISLQKLASEDPSFRVRTDEETGQTIISGMGELHLEIIVDRLMREFKVEANVGKPQV
HHHCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AYRETVTKKVKVEGKFVRQSGGRGQYGHVWIELEPQEAGKGYEFVDAIKGGVVPREYIPA
HHHHHHHHHEEECEEEEECCCCCCCCCEEEEEEEHHHCCCCCHHHHHHHCCCCCHHHHHH
VDKGIQEAMETGVLAGYPTVDFKVALVDGSYHEVDSSEMAFKIAGSMAFKEAAAKAGPVL
HHHHHHHHHHCCCEECCCCCEEEEEEECCCCCCCCCCHHEEEHHHHHHHHHHHHHCCCHH
LEPIMSVEVVVPEEYMGDVIGDLNSRRGRIMGMEGRAGAQVVSAMVPLAQMFGYATDLRS
HHCCCEEEEEECHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATQGRATYTMTFDHYEQVPKSVSEEIVAKVKG
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA