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The map label for this gene is nifK [H]

Identifier: 39997912

GI number: 39997912

Start: 3100763

End: 3102232

Strand: Reverse

Name: nifK [H]

Synonym: GSU2819

Alternate gene names: 39997912

Gene position: 3102232-3100763 (Counterclockwise)

Preceding gene: 39997913

Following gene: 39997910

Centisome position: 81.34

GC content: 61.56

Gene sequence:

>1470_bases
ATGAGCAACCAACTCGGACTCGCCGTCAAGCCGGTCACCGAATACGATGACGCAGAAGTAAAGAGAGTCGCCGAATGGAT
CAACACTGAAGAGTACAAGGAGAAGAACTTCGCCCGCCAGGCCCTGGTGATCAACCCGGCCCACGCCTGTCAGCCCCTGG
GGGCCGAACTGGTGGCCCACGCCTTCGAGGGGACCCTGCCATTCGTTCACGGTTCCCAGGGGTGCGCCTCCTACTACCGC
TCCACCCTCAACCGGCACTTCCGGGAGCCGGCGCCGGCCGTTTCCGATGCCATGACCGAGGACGGCGCCGTGTTCGGCGG
CCAGAACAACCTCCACGAGGGGCTGGAAAACGCCATCGCTCTCTACAAGCCCAAGATGGTCGCCGTCTTCACCTCGTGCA
TGCCGGAGATCATCGGCGACGACCTGACCGCGTTCCTGAAGAACGCCCGCAACAAGGGGATCATCCCGGCGGACATGCCG
ACCCCGTACGCCAACACCCCGAGCTTCAACGGCTCACACATCCACGGCTACGACGCCATGCTCCTTTCCATCCTGCAGAC
TCTGACCGCGGGCAAACAGGTGGAGGGTCGCTGCACGGGCAAGCTTAACCTGATCCCCGGCTTCGACGCCAATACCGGCA
ACTTCAGGGAGTACAAGCGGATTCTCGAGGCCTTCGGCATTCCCTACACCATCCTCGGCGACATCTCCGACGTGTTCGAT
TCGCCCCTGGACGGCACGTACCGCCCCTATCCGGGCGGCACCACGCTGGATGACGCCGCCGACTCCATCAACGGCAAGGC
CACCCTCAACCTGGGGCCCTATTCGGCGGCAAAGACCTTCTCTTGGGTTAAAGACTCCTATTCCGGTAAGCATGCGTCCC
TTCCCATGCCCATGGGAGTCACCAAGACCGACGACTTCCTCAAGAAGCTGTCGGAGCTCTTCGGCAAGCCGGTCCCCGAG
AGTCTGAAGGAGGAGCGGGGCCGGGCCGTGGACGCCATGACCGATGCCCACCAGTACATCCACAACAAAAAGTTCGCCGT
CTACGGCGATCCCGACCAGCTCCTCGGCTACGTCTCCTTTCTGCTGGAGATGGGCGCCAAGCCCTATCACATCCTCTGCA
GCAAGGGGACAAAGAAGCTGGAGAAGGAAATCCAGGCGTTGCTCGATACCTCTCCCTACGGCGCCGGCTGCAAGATCTAC
ATCAACAAGGATCTCTGGCACATGCGGAGCCTGCTCATGACCGACCCGGTGGACGCCATGATCGGTGACACCCACGGCAA
GTTCGCGGCCCGCGACGCCGGTATCCCGCTTTTCCGCTTCGGCTTCCCGATCTTCGACCGGGTCAACAAGCACCGCTACC
CGATCATCGGCTACCAGGGCGTGGTCAATATGCTGACCGAGATCTGCAACAAGTTCCTCGACATCACCGACGAGACTTGT
GAGGACCGGTTCTTCGAGATGATGCGGTAA

Upstream 100 bases:

>100_bases
TCGGCGCCACGGTCCTGACGAAAATCGGCGTCGGATGGAAAAAACAAAACCAAACCCGGTCGTGGGACGGATTGGTCCCC
GGCCGTAAAGGAGAATCGCC

Downstream 100 bases:

>100_bases
GTTGACTGCGTAGGTGCCTGTAGCTCTGCAGGCTGTGAGTGGGAGCCGGATAGGGATTCCACTTGCAGCCTGCAGAAATG
TATGATGTCGGCACAGTATG

Product: nitrogenase molybdenum-iron protein subunit beta

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSNQLGLAVKPVTEYDDAEVKRVAEWINTEEYKEKNFARQALVINPAHACQPLGAELVAHAFEGTLPFVHGSQGCASYYR
STLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAIALYKPKMVAVFTSCMPEIIGDDLTAFLKNARNKGIIPADMP
TPYANTPSFNGSHIHGYDAMLLSILQTLTAGKQVEGRCTGKLNLIPGFDANTGNFREYKRILEAFGIPYTILGDISDVFD
SPLDGTYRPYPGGTTLDDAADSINGKATLNLGPYSAAKTFSWVKDSYSGKHASLPMPMGVTKTDDFLKKLSELFGKPVPE
SLKEERGRAVDAMTDAHQYIHNKKFAVYGDPDQLLGYVSFLLEMGAKPYHILCSKGTKKLEKEIQALLDTSPYGAGCKIY
INKDLWHMRSLLMTDPVDAMIGDTHGKFAARDAGIPLFRFGFPIFDRVNKHRYPIIGYQGVVNMLTEICNKFLDITDETC
EDRFFEMMR

Sequences:

>Translated_489_residues
MSNQLGLAVKPVTEYDDAEVKRVAEWINTEEYKEKNFARQALVINPAHACQPLGAELVAHAFEGTLPFVHGSQGCASYYR
STLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAIALYKPKMVAVFTSCMPEIIGDDLTAFLKNARNKGIIPADMP
TPYANTPSFNGSHIHGYDAMLLSILQTLTAGKQVEGRCTGKLNLIPGFDANTGNFREYKRILEAFGIPYTILGDISDVFD
SPLDGTYRPYPGGTTLDDAADSINGKATLNLGPYSAAKTFSWVKDSYSGKHASLPMPMGVTKTDDFLKKLSELFGKPVPE
SLKEERGRAVDAMTDAHQYIHNKKFAVYGDPDQLLGYVSFLLEMGAKPYHILCSKGTKKLEKEIQALLDTSPYGAGCKIY
INKDLWHMRSLLMTDPVDAMIGDTHGKFAARDAGIPLFRFGFPIFDRVNKHRYPIIGYQGVVNMLTEICNKFLDITDETC
EDRFFEMMR
>Mature_488_residues
SNQLGLAVKPVTEYDDAEVKRVAEWINTEEYKEKNFARQALVINPAHACQPLGAELVAHAFEGTLPFVHGSQGCASYYRS
TLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAIALYKPKMVAVFTSCMPEIIGDDLTAFLKNARNKGIIPADMPT
PYANTPSFNGSHIHGYDAMLLSILQTLTAGKQVEGRCTGKLNLIPGFDANTGNFREYKRILEAFGIPYTILGDISDVFDS
PLDGTYRPYPGGTTLDDAADSINGKATLNLGPYSAAKTFSWVKDSYSGKHASLPMPMGVTKTDDFLKKLSELFGKPVPES
LKEERGRAVDAMTDAHQYIHNKKFAVYGDPDQLLGYVSFLLEMGAKPYHILCSKGTKKLEKEIQALLDTSPYGAGCKIYI
NKDLWHMRSLLMTDPVDAMIGDTHGKFAARDAGIPLFRFGFPIFDRVNKHRYPIIGYQGVVNMLTEICNKFLDITDETCE
DRFFEMMR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54148; Mature: 54017

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQLGLAVKPVTEYDDAEVKRVAEWINTEEYKEKNFARQALVINPAHACQPLGAELVAH
CCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHEEECCHHHCCCHHHHHHHH
AFEGTLPFVHGSQGCASYYRSTLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAIA
HHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHH
LYKPKMVAVFTSCMPEIIGDDLTAFLKNARNKGIIPADMPTPYANTPSFNGSHIHGYDAM
HHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHH
LLSILQTLTAGKQVEGRCTGKLNLIPGFDANTGNFREYKRILEAFGIPYTILGDISDVFD
HHHHHHHHHCCCCCCCEECCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEHHCHHHHHC
SPLDGTYRPYPGGTTLDDAADSINGKATLNLGPYSAAKTFSWVKDSYSGKHASLPMPMGV
CCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCC
TKTDDFLKKLSELFGKPVPESLKEERGRAVDAMTDAHQYIHNKKFAVYGDPDQLLGYVSF
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHH
LLEMGAKPYHILCSKGTKKLEKEIQALLDTSPYGAGCKIYINKDLWHMRSLLMTDPVDAM
HHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHH
IGDTHGKFAARDAGIPLFRFGFPIFDRVNKHRYPIIGYQGVVNMLTEICNKFLDITDETC
HCCCCCCCCHHCCCCCHHHHCCHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHCCCCHHH
EDRFFEMMR
HHHHHHHHC
>Mature Secondary Structure 
SNQLGLAVKPVTEYDDAEVKRVAEWINTEEYKEKNFARQALVINPAHACQPLGAELVAH
CCCCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHEEECCHHHCCCHHHHHHHH
AFEGTLPFVHGSQGCASYYRSTLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAIA
HHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHH
LYKPKMVAVFTSCMPEIIGDDLTAFLKNARNKGIIPADMPTPYANTPSFNGSHIHGYDAM
HHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHH
LLSILQTLTAGKQVEGRCTGKLNLIPGFDANTGNFREYKRILEAFGIPYTILGDISDVFD
HHHHHHHHHCCCCCCCEECCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEHHCHHHHHC
SPLDGTYRPYPGGTTLDDAADSINGKATLNLGPYSAAKTFSWVKDSYSGKHASLPMPMGV
CCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCC
TKTDDFLKKLSELFGKPVPESLKEERGRAVDAMTDAHQYIHNKKFAVYGDPDQLLGYVSF
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHH
LLEMGAKPYHILCSKGTKKLEKEIQALLDTSPYGAGCKIYINKDLWHMRSLLMTDPVDAM
HHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHH
IGDTHGKFAARDAGIPLFRFGFPIFDRVNKHRYPIIGYQGVVNMLTEICNKFLDITDETC
HCCCCCCCCHHCCCCCHHHHCCHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHCCCCHHH
EDRFFEMMR
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]