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The map label for this gene is nifEN [H]

Identifier: 39997899

GI number: 39997899

Start: 3084293

End: 3087052

Strand: Reverse

Name: nifEN [H]

Synonym: GSU2806

Alternate gene names: 39997899

Gene position: 3087052-3084293 (Counterclockwise)

Preceding gene: 39997900

Following gene: 39997898

Centisome position: 80.94

GC content: 64.46

Gene sequence:

>2760_bases
ATGGCAAGACCCGATTATTACGACACAACCGACTGCGAAACCCACGAAAAGGGTGCCCCGAAATTCTGCAAGAAATCGGA
ACCGGGGGAGGGCACCGAGCGCTCCTGTGCCTATGACGGCGCCCGGGTGGTGCTCATGCCGGTCACCGACGTGATCCATC
TGGTGCACGGCCCCATCGCCTGCGCCGGCAACTCCTGGGATAACCGGGGTGCCCGCTCTTCGGGCTCCCAGCTCTACCGC
AGGGGCTTTACCACCGAGATGCTGGAAAACGACGTGATCTTCGGCGGCGAGAAGAAGCTCTACCGGGCCATCCTGGAGCT
GGCGGAGCGCTATGCGGGCCAGGCAAAGGCCATGTTCGTCTATGCCACCTGCGTCACCGCCATGACCGGCGACGACGTGG
AGGCGGTCTGCAAGGCGGCCCAGAAAAAGGTCGCCATCCCCCTGATCCCGGTCAACACTCCCGGCTTCATCGGCGACAAG
AACATCGGCAACCGCCTGGCCGGCGAGGTGCTCCTGAAGCACGTCATCGGCACCGCCGAGCCGCCGGTGCTGGGAGAGTA
TCCCATCAACCTGATCGGCGAATACAACATCGCCGGCGACCTTTGGGGGATGCTGCCGCTGTTCGACCGGCTCGGTATCC
AGATTCTCTCCTGTTTCAGCGGTGACGCCAAATTCGAGGATCTCCGCTACGCCCACCGGGCGAAGCTGAACGTCATCATC
TGCTCCAAGAGTCTCACCAACCTCGCCAGGAAGATGCAGAAGAACTACGGCATGCCCTACCTGGAGGAGTCCTTTTACGG
CATGACCGACACGGCCAAGGCCCTGCGGGACATCGCCCGGGAGCTGGATGACGCCGTGGGCGGGCTGGAGAAGCGGATCA
TGCAGGACCGGGTGGAGAAGCTCCTGGAAGAGGAAGAGGCGCAATGCCGGGAGCGGCTTGCCCCCTACCGGGCACGGCTC
GAAGGGAAGCGCTCGGTTCTTTTCACCGGCGGGGTCAAGACCTGGTCCATGGTGAACGCCCTGCGGGAGCTGGGGGTGGA
GATCCTGGCCGCCGGCACCCAGAACTCGACCCTGGAGGATTTCTACCGGATGAAGGCCCTCATGCACCAGGATGCCGGGA
TCATCGAGGACACTTCCAGCGCCGGGCTCCTCAAGGTCATGTATGACAAGATGCCCGATCTGATCGTTGCCGGCGGGAAG
ACCAAGTTCCTGGCCCTGAAAACCAAGACGCCGTTCCTGGATATCAACCATGGGCGTTCCCATCCCTATGCGGGGTACGA
GGGGATGGTGACCTTTGCGAAGCAGCTCGACCTGACGGTAAACAACCCCATCTGGCCAGTGTTGAACGCCAAGTCCCCCT
GGGAGAAGTCCGATGAGGAACTGGCTGCGAGCGTGGCCAGGGCGGCCGGCCATGCCCGGGCCTACCTGGACGAGGACCTG
AAGGATTCACGGGTCAAGGTCCCCACCAAGCCGGCCACGGTGAACCCCCAGAAGAATTCGCCTGCCCTGGGCGCAACGTT
GGCTTACCTGGGCATCGACCAGATGCTGGCGCTCCTGCACGGAGCCCAGGGCTGCTCCACCTTCATCCGGCTCCAGCTCT
CCCGCCACTTCAAGGAGCCCATCGCCCTCAACTCCACCGCCATGAGCGAGGACACCGCCATCTTCGGCGGCTGGGAGAAC
CTGAAGGCGGGGCTCGCGCGGGTCATGGAGAAATTCAGGCCAACGGTGGTCGGCGTCATGACCTCAGGTCTCACGGAAAC
CATGGGGGATGACGTCCGGAGCGCCATCCACCAGTTCCGGGAGGAGCACCCGGAGCATGACAACGTGCCCGTGGTTTGGG
CGTCCACGCCCGACTACTGCGGTTCGCTCCAGGAGGGATACGCGGCGGCGGTGGAGGCCATTGTCCGGAGCGTGCCGGAG
CCCGGCGCATCCATCCCGGACCAGGTGACGGTTCTCCCCGGCGCCCACCTGACCCCCGCCGATGTGGAGGAGGTCCGGGA
GATCTGCGAGGCCTTCGGGCTCGATCCCATCATTGTGCCGGACATCGCCAATGCCCTGGACGGCCATATCGACGAAACGG
TTTCGCCCCTGTCAACCGGGGGTGTCTCCCCCGAGCGGATCCGTCAGGCCGGCCGCAGCGCCGCCGCCATCTTCATCGGC
GACTCGCTGGCAAAGGCGGCAGAAGCGCTCACCACCACCTGCGGCATGCCCAACTACGGTTTCACCTCCCTCACGGGCCT
GGCCGAGGTCGACCGCTTCATGGAAACCCTGTCGGCCATTTCGGGCCGGCCGATCCCGGAGAAGTTCAACCGCTGGCGGA
GCCGGCTCATGGACGCCATGGTCGACTCCCACTACCAGTTCGGGCTGAAGAAGGTTACCGTTGCCCTGGAAGGCGACAAC
CTGAAGGTTCTGACGAATTTCCTGGCAGGCATGGGATGCGAGATCCGGGCGGCCATTGCCGCCACCAGGGTCCGGGGGCT
GGACGCCCTCCCGGCCGGGGACGTCTTCGTGGGCGACCTGGAAGACCTGGAGACGACCGCCAAGGGGTGCGACCTGATCG
TAGCCAACTCCAACGGCCGCCAGGCCGCGGCCAAACTCGGCATCAAGGCCCATCTGCGGGCCGGGCTTCCGGTTTTCGAC
CGCCTCGGCGCCCACCAGAAAATGTGGGTTGGATACCGGGGAACCATGAATCTGCTGTTCGAAACGGCCAACCTGTTCCA
GGCCAACGCGGGGGATGCCCAGAAGCTGGCCCATAACTGA

Upstream 100 bases:

>100_bases
TTTGAACCATCAGTGAGCAGGGCAGAGACCGACGCGGCAACGACGCCGCCATTCTCCGGAATGGCGGCGTTTTCTTTTGC
AGGAAACATTCGAGGTCACC

Downstream 100 bases:

>100_bases
TTCGAGCCGAAATGCCTGCCGACTTCGCCGCGGCGCTGCCCGCACGCGCGAATCGGGCGGAGCTCAAGAAAGGAAGAACA
CATGAAAGTCGCATTCACAA

Product: bifunctional nitrogenase molybdenum-cofactor biosynthesis protein NifE/NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 919; Mature: 918

Protein sequence:

>919_residues
MARPDYYDTTDCETHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDVIHLVHGPIACAGNSWDNRGARSSGSQLYR
RGFTTEMLENDVIFGGEKKLYRAILELAERYAGQAKAMFVYATCVTAMTGDDVEAVCKAAQKKVAIPLIPVNTPGFIGDK
NIGNRLAGEVLLKHVIGTAEPPVLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEDLRYAHRAKLNVII
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEKLLEEEEAQCRERLAPYRARL
EGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHQDAGIIEDTSSAGLLKVMYDKMPDLIVAGGK
TKFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKSPWEKSDEELAASVARAAGHARAYLDEDL
KDSRVKVPTKPATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEPIALNSTAMSEDTAIFGGWEN
LKAGLARVMEKFRPTVVGVMTSGLTETMGDDVRSAIHQFREEHPEHDNVPVVWASTPDYCGSLQEGYAAAVEAIVRSVPE
PGASIPDQVTVLPGAHLTPADVEEVREICEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSPERIRQAGRSAAAIFIG
DSLAKAAEALTTTCGMPNYGFTSLTGLAEVDRFMETLSAISGRPIPEKFNRWRSRLMDAMVDSHYQFGLKKVTVALEGDN
LKVLTNFLAGMGCEIRAAIAATRVRGLDALPAGDVFVGDLEDLETTAKGCDLIVANSNGRQAAAKLGIKAHLRAGLPVFD
RLGAHQKMWVGYRGTMNLLFETANLFQANAGDAQKLAHN

Sequences:

>Translated_919_residues
MARPDYYDTTDCETHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDVIHLVHGPIACAGNSWDNRGARSSGSQLYR
RGFTTEMLENDVIFGGEKKLYRAILELAERYAGQAKAMFVYATCVTAMTGDDVEAVCKAAQKKVAIPLIPVNTPGFIGDK
NIGNRLAGEVLLKHVIGTAEPPVLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEDLRYAHRAKLNVII
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEKLLEEEEAQCRERLAPYRARL
EGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHQDAGIIEDTSSAGLLKVMYDKMPDLIVAGGK
TKFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKSPWEKSDEELAASVARAAGHARAYLDEDL
KDSRVKVPTKPATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEPIALNSTAMSEDTAIFGGWEN
LKAGLARVMEKFRPTVVGVMTSGLTETMGDDVRSAIHQFREEHPEHDNVPVVWASTPDYCGSLQEGYAAAVEAIVRSVPE
PGASIPDQVTVLPGAHLTPADVEEVREICEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSPERIRQAGRSAAAIFIG
DSLAKAAEALTTTCGMPNYGFTSLTGLAEVDRFMETLSAISGRPIPEKFNRWRSRLMDAMVDSHYQFGLKKVTVALEGDN
LKVLTNFLAGMGCEIRAAIAATRVRGLDALPAGDVFVGDLEDLETTAKGCDLIVANSNGRQAAAKLGIKAHLRAGLPVFD
RLGAHQKMWVGYRGTMNLLFETANLFQANAGDAQKLAHN
>Mature_918_residues
ARPDYYDTTDCETHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDVIHLVHGPIACAGNSWDNRGARSSGSQLYRR
GFTTEMLENDVIFGGEKKLYRAILELAERYAGQAKAMFVYATCVTAMTGDDVEAVCKAAQKKVAIPLIPVNTPGFIGDKN
IGNRLAGEVLLKHVIGTAEPPVLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEDLRYAHRAKLNVIIC
SKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEKLLEEEEAQCRERLAPYRARLE
GKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHQDAGIIEDTSSAGLLKVMYDKMPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKSPWEKSDEELAASVARAAGHARAYLDEDLK
DSRVKVPTKPATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEPIALNSTAMSEDTAIFGGWENL
KAGLARVMEKFRPTVVGVMTSGLTETMGDDVRSAIHQFREEHPEHDNVPVVWASTPDYCGSLQEGYAAAVEAIVRSVPEP
GASIPDQVTVLPGAHLTPADVEEVREICEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSPERIRQAGRSAAAIFIGD
SLAKAAEALTTTCGMPNYGFTSLTGLAEVDRFMETLSAISGRPIPEKFNRWRSRLMDAMVDSHYQFGLKKVTVALEGDNL
KVLTNFLAGMGCEIRAAIAATRVRGLDALPAGDVFVGDLEDLETTAKGCDLIVANSNGRQAAAKLGIKAHLRAGLPVFDR
LGAHQKMWVGYRGTMNLLFETANLFQANAGDAQKLAHN

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 100019; Mature: 99887

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPDYYDTTDCETHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDVIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSGSQLYRRGFTTEMLENDVIFGGEKKLYRAILELAERYAGQAKAMFV
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHHCCCHHHEEH
YATCVTAMTGDDVEAVCKAAQKKVAIPLIPVNTPGFIGDKNIGNRLAGEVLLKHVIGTAE
HHHHHHHHCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPVLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEDLRYAHRAKLNVII
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEEEE
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEK
ECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEEEEAQCRERLAPYRARLEGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLED
HHHHHHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHH
FYRMKALMHQDAGIIEDTSSAGLLKVMYDKMPDLIVAGGKTKFLALKTKTPFLDINHGRS
HHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCEEEECCCEEEEEEECCCCEEECCCCCC
HPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKSPWEKSDEELAASVARAAGHARAYLDEDL
CCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KDSRVKVPTKPATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEP
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
IALNSTAMSEDTAIFGGWENLKAGLARVMEKFRPTVVGVMTSGLTETMGDDVRSAIHQFR
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEHPEHDNVPVVWASTPDYCGSLQEGYAAAVEAIVRSVPEPGASIPDQVTVLPGAHLTPA
HHCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
DVEEVREICEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSPERIRQAGRSAAAIFIG
CHHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHCCHHCCCCCHHHHHHCCCCEEEEEEC
DSLAKAAEALTTTCGMPNYGFTSLTGLAEVDRFMETLSAISGRPIPEKFNRWRSRLMDAM
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VDSHYQFGLKKVTVALEGDNLKVLTNFLAGMGCEIRAAIAATRVRGLDALPAGDVFVGDL
HHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEECCH
EDLETTAKGCDLIVANSNGRQAAAKLGIKAHLRAGLPVFDRLGAHQKMWVGYRGTMNLLF
HHHHHHHCCCEEEEECCCCHHHHHHHCHHHHHHCCCCHHHHCCCCCEEEEECCHHHHHHH
ETANLFQANAGDAQKLAHN
HHHHHHCCCCCCHHHHCCC
>Mature Secondary Structure 
ARPDYYDTTDCETHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDVIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSGSQLYRRGFTTEMLENDVIFGGEKKLYRAILELAERYAGQAKAMFV
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHHCCCHHHEEH
YATCVTAMTGDDVEAVCKAAQKKVAIPLIPVNTPGFIGDKNIGNRLAGEVLLKHVIGTAE
HHHHHHHHCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPVLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEDLRYAHRAKLNVII
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEEEE
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEK
ECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEEEEAQCRERLAPYRARLEGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLED
HHHHHHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHH
FYRMKALMHQDAGIIEDTSSAGLLKVMYDKMPDLIVAGGKTKFLALKTKTPFLDINHGRS
HHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCEEEECCCEEEEEEECCCCEEECCCCCC
HPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKSPWEKSDEELAASVARAAGHARAYLDEDL
CCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KDSRVKVPTKPATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEP
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
IALNSTAMSEDTAIFGGWENLKAGLARVMEKFRPTVVGVMTSGLTETMGDDVRSAIHQFR
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEHPEHDNVPVVWASTPDYCGSLQEGYAAAVEAIVRSVPEPGASIPDQVTVLPGAHLTPA
HHCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
DVEEVREICEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSPERIRQAGRSAAAIFIG
CHHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHCCHHCCCCCHHHHHHCCCCEEEEEEC
DSLAKAAEALTTTCGMPNYGFTSLTGLAEVDRFMETLSAISGRPIPEKFNRWRSRLMDAM
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VDSHYQFGLKKVTVALEGDNLKVLTNFLAGMGCEIRAAIAATRVRGLDALPAGDVFVGDL
HHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEECCH
EDLETTAKGCDLIVANSNGRQAAAKLGIKAHLRAGLPVFDRLGAHQKMWVGYRGTMNLLF
HHHHHHHCCCEEEEECCCCHHHHHHHCHHHHHHCCCCHHHHCCCCCEEEEECCHHHHHHH
ETANLFQANAGDAQKLAHN
HHHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]