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The map label for this gene is mscL

Identifier: 39997887

GI number: 39997887

Start: 3072594

End: 3073025

Strand: Direct

Name: mscL

Synonym: GSU2794

Alternate gene names: 39997887

Gene position: 3072594-3073025 (Clockwise)

Preceding gene: 39997885

Following gene: 39997893

Centisome position: 80.56

GC content: 58.8

Gene sequence:

>432_bases
ATGTTCAAGGAGTTCAAGGAATTCGCCATGAAGGGAAATGTGGTCGATCTGGCCATCGGCGTTATCATCGGCGGCGCATT
CGGCAAGATCGTCACGTCGGTGGTCAATGATATCGTGATGCCTCCCATCGGCCTCCTGATGGGCAAAATGGACTTCTCGA
ACCTTTTCATCGACCTGTCGGGCAAGGGCTACGAGAGCCTCAAGGCCGCCAAGGACGCAGGCGCGCCGGTCATCAGCTAC
GGTGCCTTCATCAACACGGTCCTGGACTTCGTCATCGTGGCCTTCGTGATCTTCCTGGTGATCAAGCAGATCAACCGCCT
GAAAAAGGAGCCGGTGCCCGCCCCGCCCGACACCAAGGAGTGCGCCTTCTGCTGCTCCGCCATCCCCATCAAGGCCACCC
GCTGCCCCCACTGCACGTCGGAACAGAAATAG

Upstream 100 bases:

>100_bases
CTGCAAAAAGAGGCACGTTCTCCCTTCCAATCGGGCGGGGATGTGGTACAACAAAGGTTATTGCCGGCCCAACGACCAGC
CCACTACACGGAGGTGCATG

Downstream 100 bases:

>100_bases
CTTTATCCGGGCGGAGACACGATTACCGCTTCAACGGCTTGATGGCACGAAAAAGGGCGCTCCGCCGGGAGCGCCCTTTT
TCATTGCCAACCACTGCCGA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MFKEFKEFAMKGNVVDLAIGVIIGGAFGKIVTSVVNDIVMPPIGLLMGKMDFSNLFIDLSGKGYESLKAAKDAGAPVISY
GAFINTVLDFVIVAFVIFLVIKQINRLKKEPVPAPPDTKECAFCCSAIPIKATRCPHCTSEQK

Sequences:

>Translated_143_residues
MFKEFKEFAMKGNVVDLAIGVIIGGAFGKIVTSVVNDIVMPPIGLLMGKMDFSNLFIDLSGKGYESLKAAKDAGAPVISY
GAFINTVLDFVIVAFVIFLVIKQINRLKKEPVPAPPDTKECAFCCSAIPIKATRCPHCTSEQK
>Mature_143_residues
MFKEFKEFAMKGNVVDLAIGVIIGGAFGKIVTSVVNDIVMPPIGLLMGKMDFSNLFIDLSGKGYESLKAAKDAGAPVISY
GAFINTVLDFVIVAFVIFLVIKQINRLKKEPVPAPPDTKECAFCCSAIPIKATRCPHCTSEQK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=123, Percent_Identity=56.0975609756098, Blast_Score=126, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_GEOSL (Q749E9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953838.1
- ProteinModelPortal:   Q749E9
- SMR:   Q749E9
- GeneID:   2686216
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2794
- NMPDR:   fig|243231.1.peg.2776
- TIGR:   GSU2794
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- ProtClustDB:   PRK00567
- BioCyc:   GSUL243231:GSU_2794-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15431; Mature: 15431

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10a7e3c4)-; HASH(0x115cc4a4)-;

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFKEFAMKGNVVDLAIGVIIGGAFGKIVTSVVNDIVMPPIGLLMGKMDFSNLFIDLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECC
GKGYESLKAAKDAGAPVISYGAFINTVLDFVIVAFVIFLVIKQINRLKKEPVPAPPDTKE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CAFCCSAIPIKATRCPHCTSEQK
HHHHHHCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MFKEFKEFAMKGNVVDLAIGVIIGGAFGKIVTSVVNDIVMPPIGLLMGKMDFSNLFIDLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECC
GKGYESLKAAKDAGAPVISYGAFINTVLDFVIVAFVIFLVIKQINRLKKEPVPAPPDTKE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CAFCCSAIPIKATRCPHCTSEQK
HHHHHHCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA