Click here to switch to the map view.

The map label for this gene is rsmF [H]

Identifier: 39997843

GI number: 39997843

Start: 3024642

End: 3025601

Strand: Direct

Name: rsmF [H]

Synonym: GSU2749

Alternate gene names: 39997843

Gene position: 3024642-3025601 (Clockwise)

Preceding gene: 39997842

Following gene: 39997846

Centisome position: 79.3

GC content: 68.33

Gene sequence:

>960_bases
ATGACAGACACCGGTCTGCCGCCCCTTCCCGTCCCGTTCCTGGCACTCATGGAGGAAGTGCTCCCTCCGGACCTGCGCGA
CGCATGCCTGGCCAGCTTCAGCCAGGAGAAGCCCACCTCGCTGCGTCTCAACCCCCTGAAGGGCGACCCGGACGAGACCC
GTCGGGAGCTGGAAAGCGCCGGATTCGACCCGGTGCCGGTATCCTGGTATCCCCTGGCTTTCACCGTTGACGGCGAGAGG
CGCCGCGCCCTGACCGAGTCGCCCCCCTTCCGGGAGGGGCGTATTTACATCCAGAGCCTCTCCAGCATGACCGCGCCCCT
GGCCCTCGCGCCCGAGCCGGGGGAGACGGTGCTTGACCTGGCTGCCGCTCCCGGCGGCAAAACTGCCATGATGGCCGCCA
TGATGGAAAACCGGGGACGCCTGTCGGCCGTGGAGGCGGTGCGAGGGCGCTTCTTCCGGATGAAGGCGAACCTGGACCAT
CAGGGGGCCACCATGGTGCGGACCTACCTGACCGATGGCCGGAGCGTGGGACGCAAGTGCCCGGAGATGTTCGACCGGGT
GCTGCTGGACGCGCCCTGTTCCTCCGAGGCCCGCTTCACGCGCCTGAACCCGGAGAGCTGGGCCCACTGGAGCCCCCGCA
AGGTGGAGGAAGCGGCCCGCAAGCAACGGGGGCTCATCCGCTCGGCCCTGGAGGCCCTGCGTCCGGGCGGACTGCTGCTT
TACTGCACCTGCTCCTTCTCGCCGGAGGAGAACGAACTGATCGTCAACGATGTCCTGCGCCGGGATGGGGACAACCTCCG
GGTCTGTGCCGTGGACCTGCCCCTGCCGAACCGCCGGCCGGGCCTCACCCGCTGGAAGGGGAAGGAGCTCCATCCCGACC
TGGCGCGGGCGGTCCGTATCCTCCCCGACCGGCTGATGGACGGCTTTTTCCTCTGCCTCATCCGCAAATCGTCTGATTGA

Upstream 100 bases:

>100_bases
CCAGGGAAGGGATCAAGGGGCTGAAGCGGCGGCTTTCCCCGCCGGAGCACGATGATACCCTCTACCGCCTCCCCGTGGGC
GATGAGGAGGCAGGCACCCC

Downstream 100 bases:

>100_bases
TGAACCCGTCTTCGGCGGGGACGCTCGTGGGTAGGAGTGATGAACTGCCCGGGCGGGCAGTGCAAACAAGGGATCATTCG
CTGCCTGCCAGAAGTTCGAG

Product: NOL1/NOP2/sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTDTGLPPLPVPFLALMEEVLPPDLRDACLASFSQEKPTSLRLNPLKGDPDETRRELESAGFDPVPVSWYPLAFTVDGER
RRALTESPPFREGRIYIQSLSSMTAPLALAPEPGETVLDLAAAPGGKTAMMAAMMENRGRLSAVEAVRGRFFRMKANLDH
QGATMVRTYLTDGRSVGRKCPEMFDRVLLDAPCSSEARFTRLNPESWAHWSPRKVEEAARKQRGLIRSALEALRPGGLLL
YCTCSFSPEENELIVNDVLRRDGDNLRVCAVDLPLPNRRPGLTRWKGKELHPDLARAVRILPDRLMDGFFLCLIRKSSD

Sequences:

>Translated_319_residues
MTDTGLPPLPVPFLALMEEVLPPDLRDACLASFSQEKPTSLRLNPLKGDPDETRRELESAGFDPVPVSWYPLAFTVDGER
RRALTESPPFREGRIYIQSLSSMTAPLALAPEPGETVLDLAAAPGGKTAMMAAMMENRGRLSAVEAVRGRFFRMKANLDH
QGATMVRTYLTDGRSVGRKCPEMFDRVLLDAPCSSEARFTRLNPESWAHWSPRKVEEAARKQRGLIRSALEALRPGGLLL
YCTCSFSPEENELIVNDVLRRDGDNLRVCAVDLPLPNRRPGLTRWKGKELHPDLARAVRILPDRLMDGFFLCLIRKSSD
>Mature_318_residues
TDTGLPPLPVPFLALMEEVLPPDLRDACLASFSQEKPTSLRLNPLKGDPDETRRELESAGFDPVPVSWYPLAFTVDGERR
RALTESPPFREGRIYIQSLSSMTAPLALAPEPGETVLDLAAAPGGKTAMMAAMMENRGRLSAVEAVRGRFFRMKANLDHQ
GATMVRTYLTDGRSVGRKCPEMFDRVLLDAPCSSEARFTRLNPESWAHWSPRKVEEAARKQRGLIRSALEALRPGGLLLY
CTCSFSPEENELIVNDVLRRDGDNLRVCAVDLPLPNRRPGLTRWKGKELHPDLARAVRILPDRLMDGFFLCLIRKSSD

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=288, Percent_Identity=32.6388888888889, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI76150623, Length=288, Percent_Identity=32.6388888888889, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI32698918, Length=170, Percent_Identity=34.7058823529412, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI11545785, Length=182, Percent_Identity=34.0659340659341, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI39995082, Length=310, Percent_Identity=27.0967741935484, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI301336155, Length=211, Percent_Identity=27.9620853080569, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270288818, Length=239, Percent_Identity=30.5439330543933, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23199998, Length=239, Percent_Identity=30.5439330543933, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI8922322, Length=239, Percent_Identity=30.5439330543933, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI270288816, Length=239, Percent_Identity=30.5439330543933, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI40316918, Length=224, Percent_Identity=32.5892857142857, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI87081985, Length=318, Percent_Identity=34.5911949685535, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI2367212, Length=268, Percent_Identity=31.7164179104478, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=290, Percent_Identity=33.7931034482759, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25143471, Length=315, Percent_Identity=27.6190476190476, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=245, Percent_Identity=26.1224489795918, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=168, Percent_Identity=30.3571428571429, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=286, Percent_Identity=30.0699300699301, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319447, Length=327, Percent_Identity=29.3577981651376, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI22024126, Length=294, Percent_Identity=30.6122448979592, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24668781, Length=204, Percent_Identity=31.8627450980392, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356513, Length=207, Percent_Identity=31.8840579710145, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355201, Length=258, Percent_Identity=28.6821705426357, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920494, Length=177, Percent_Identity=29.9435028248588, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356579, Length=238, Percent_Identity=27.3109243697479, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 35597; Mature: 35466

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTGLPPLPVPFLALMEEVLPPDLRDACLASFSQEKPTSLRLNPLKGDPDETRRELESA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHC
GFDPVPVSWYPLAFTVDGERRRALTESPPFREGRIYIQSLSSMTAPLALAPEPGETVLDL
CCCCCCCEEEEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHH
AAAPGGKTAMMAAMMENRGRLSAVEAVRGRFFRMKANLDHQGATMVRTYLTDGRSVGRKC
HCCCCCHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHCCHHHHHHH
PEMFDRVLLDAPCSSEARFTRLNPESWAHWSPRKVEEAARKQRGLIRSALEALRPGGLLL
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YCTCSFSPEENELIVNDVLRRDGDNLRVCAVDLPLPNRRPGLTRWKGKELHPDLARAVRI
EEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LPDRLMDGFFLCLIRKSSD
HHHHHHHHEEEEEEECCCC
>Mature Secondary Structure 
TDTGLPPLPVPFLALMEEVLPPDLRDACLASFSQEKPTSLRLNPLKGDPDETRRELESA
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHC
GFDPVPVSWYPLAFTVDGERRRALTESPPFREGRIYIQSLSSMTAPLALAPEPGETVLDL
CCCCCCCEEEEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHH
AAAPGGKTAMMAAMMENRGRLSAVEAVRGRFFRMKANLDHQGATMVRTYLTDGRSVGRKC
HCCCCCHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHCCHHHHHHH
PEMFDRVLLDAPCSSEARFTRLNPESWAHWSPRKVEEAARKQRGLIRSALEALRPGGLLL
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YCTCSFSPEENELIVNDVLRRDGDNLRVCAVDLPLPNRRPGLTRWKGKELHPDLARAVRI
EEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LPDRLMDGFFLCLIRKSSD
HHHHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA