Click here to switch to the map view.

The map label for this gene is pncA [H]

Identifier: 39997388

GI number: 39997388

Start: 2508305

End: 2508910

Strand: Direct

Name: pncA [H]

Synonym: GSU2290

Alternate gene names: 39997388

Gene position: 2508305-2508910 (Clockwise)

Preceding gene: 39997387

Following gene: 39997389

Centisome position: 65.76

GC content: 63.04

Gene sequence:

>606_bases
ATGGAAAACGATGCCGCCCTTCTCATCGTCGACGTCCAGAATGACTTCTGCCCAGGCGGAAGTCTGGCCGTCCCCGAGGG
GGACACCGTGGTTCCTGTCCTCAACGGCTATATCAGTGTCTTCCGCACAGCCGGACTCCCCATCTTCGCCTCACGGGACT
GGCACCCGCGGATGACGTCCCATTTCAAGGAACACGGCGGGCAGTGGCCGGTCCATTGCGTGCAGGGAAGCCACGGCGCA
CAGTTTCATCCAGACCTGGCACTTCCCGGCAACGCTATCGTCATATCGAAGGGAATGGATCCGGAACGGGACGACTATTC
GGCGTTCCAGGGAACTGCGGCCGACGGGACGCCCCTCCCGACCCTGCTCGCCGCCCGTGGCATCCGCCATCTGTACCTGG
GAGGACTTGCCACCGATTACTGCGTGAAGGAATCGGCTCTCGAAGGAATCCGGCACGGTCTCATCGTCACTGTGCTGACC
GATGCATCACGGGGAGTCGATCTCGCCCCCGGCGACTCGGAGCGGGCGGTGCAGGAAATGATGCGGGCGGGGGTGCGGAT
GACATCCCTTGAGGGCATCAGACAGGAACAACGGCTGCGGGAGTGA

Upstream 100 bases:

>100_bases
GTTTCCATCAGCCAAGGGCTCAATGACCTGCGACTTCGCCTCATGGAGGAGGTGCGGCACGGGTATCACGACATTGCCAA
TCCGTAAAGGAGCATATGCC

Downstream 100 bases:

>100_bases
AAATACGACGAAAAGGTATGGTATCGGATGACTCGTAGTTCTGGCAAAAACCGGAAAAACGTGCTATTGCTACCCCAACT
CGACCATTTCATCGTATTTT

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MENDAALLIVDVQNDFCPGGSLAVPEGDTVVPVLNGYISVFRTAGLPIFASRDWHPRMTSHFKEHGGQWPVHCVQGSHGA
QFHPDLALPGNAIVISKGMDPERDDYSAFQGTAADGTPLPTLLAARGIRHLYLGGLATDYCVKESALEGIRHGLIVTVLT
DASRGVDLAPGDSERAVQEMMRAGVRMTSLEGIRQEQRLRE

Sequences:

>Translated_201_residues
MENDAALLIVDVQNDFCPGGSLAVPEGDTVVPVLNGYISVFRTAGLPIFASRDWHPRMTSHFKEHGGQWPVHCVQGSHGA
QFHPDLALPGNAIVISKGMDPERDDYSAFQGTAADGTPLPTLLAARGIRHLYLGGLATDYCVKESALEGIRHGLIVTVLT
DASRGVDLAPGDSERAVQEMMRAGVRMTSLEGIRQEQRLRE
>Mature_201_residues
MENDAALLIVDVQNDFCPGGSLAVPEGDTVVPVLNGYISVFRTAGLPIFASRDWHPRMTSHFKEHGGQWPVHCVQGSHGA
QFHPDLALPGNAIVISKGMDPERDDYSAFQGTAADGTPLPTLLAARGIRHLYLGGLATDYCVKESALEGIRHGLIVTVLT
DASRGVDLAPGDSERAVQEMMRAGVRMTSLEGIRQEQRLRE

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=208, Percent_Identity=43.75, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI193206735, Length=229, Percent_Identity=28.3842794759825, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17543130, Length=229, Percent_Identity=27.9475982532751, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321401, Length=211, Percent_Identity=27.4881516587678, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648247, Length=201, Percent_Identity=29.3532338308458, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21699; Mature: 21699

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDAALLIVDVQNDFCPGGSLAVPEGDTVVPVLNGYISVFRTAGLPIFASRDWHPRMTS
CCCCEEEEEEECCCCCCCCCCEECCCCCEEHHHHHHHHHHHHHCCCCEEECCCCCCHHHH
HFKEHGGQWPVHCVQGSHGAQFHPDLALPGNAIVISKGMDPERDDYSAFQGTAADGTPLP
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHH
TLLAARGIRHLYLGGLATDYCVKESALEGIRHGLIVTVLTDASRGVDLAPGDSERAVQEM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHH
MRAGVRMTSLEGIRQEQRLRE
HHCCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MENDAALLIVDVQNDFCPGGSLAVPEGDTVVPVLNGYISVFRTAGLPIFASRDWHPRMTS
CCCCEEEEEEECCCCCCCCCCEECCCCCEEHHHHHHHHHHHHHCCCCEEECCCCCCHHHH
HFKEHGGQWPVHCVQGSHGAQFHPDLALPGNAIVISKGMDPERDDYSAFQGTAADGTPLP
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHH
TLLAARGIRHLYLGGLATDYCVKESALEGIRHGLIVTVLTDASRGVDLAPGDSERAVQEM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHH
MRAGVRMTSLEGIRQEQRLRE
HHCCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]