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The map label for this gene is metG [H]

Identifier: 39997330

GI number: 39997330

Start: 2447694

End: 2449226

Strand: Direct

Name: metG [H]

Synonym: GSU2232

Alternate gene names: 39997330

Gene position: 2447694-2449226 (Clockwise)

Preceding gene: 39997329

Following gene: 39997332

Centisome position: 64.17

GC content: 59.43

Gene sequence:

>1533_bases
ATGAGCCGAACGTTCTATGTCACGACACCCATCTACTACGTAAACGACGTACCGCACATCGGCCACGCCTACACCACCCT
TGCAGCAGACGTTCTCGCCCGCTACAAGAAGCTCAAGGGGTACGAGGTCTTCTTCCTCACCGGCACCGACGAACATGGCC
AGAAGGTCGAAAAGGCGGCCAACACCGCCGGCGAAACGCCCCTGGAGCTGGCCGACCGGGTGGTCAAGCGCTTCCAGGGC
CTCTGGGAAAAACTGGACATCCAGTATACCGACTTCATCCGCACGACCCAGGAACGGCATAAAAAAGGGGTCTCGCACAT
CTTTACCAAAATCATGGCCCAAGGTGACATCTACCTGGGTGAATATGAAGACTGGTACTGCACCCCCTGCGAAACCTTCT
GGACCGAAACCCAGCTCATCGACGGCAAATGCCCCGACTGCAACCGTCCGGTGGACAAACTCAAGGAAGAATCCTACTTC
TTCCGGATGAGCAAATATCAGGAACAACTCCTGGCCCACATCGAGGCAAATCCCGATTTCATTCAACCCAAGAGCAGGCG
CAACGAGATCATTTCCTTCGTAAAGGAAGGCCTGCGCGACCTCTCCGTCTCCCGGACCTCCTTCAACTGGGGCATTCCGG
TTCCCGGAAACGAAAAACACGTCATCTACGTCTGGTTCGATGCCCTGACCAACTACATCACGGCCTTGGGCTACCCGGAC
GAACAGAGCGCCTTCAAAACGTTCTGGCCGGTTGACGTCCACCTGATCGGCAAGGACATCCTCCGCTTCCATGCGGTCTA
CTGGCCCACATTCCTCATGGCCGCAGGCCTGCCGGTGCCCAAAAAGGTCTTTGCCCACGGATGGTGGACCGTGGAAGGCC
AGAAGATGAGCAAGAGCCTCCAGAACGTGGTGGAGCCCAACATGCTGGTGGACCGGTACGGCATTGACGCGGTCCGTTAT
TTCCTGCTGCGGGAAGTCCCCTTCGGACTCGACGGCGACTTCTCCCACGCGGCCCTTGTCCACCGGATCAACTCCGACCT
GGCGAACGATCTGGGCAACCTGCTCAACCGCTCCACCGCCATGGTCAACAAGTACTTCGACGGCGTTCTCCCCGCCCCCG
GCCCCCAGACCGAGCTGGACACCGCCTTTAGGGAAAAGACCGAAGCCATGGTGCGCCAGCTGGATGCCTGCATGGACGAA
CTGGCCTTCAGCAAGGGACTTCAGGCTATTTGGGAAGTTATCTCTGCGGGAAACAAGTACATCGACGAGACGGCACCCTG
GGCCCTGGCCAAGGACCCGGCCAAACGGGAATACCTCGGCACGGTCATGTACACGCTGCTGGAAGCCCAGCGCATCGTGC
ACCTGGTCCTCTCGGCCTTCATGCCGCGCACCTCCGCCAAGGCCCTCGGCTACCTGGGATGGACGGAGCCCTGCCGCGAA
GAAGACCTCGCCTGGGGCAGGCTGGCGCCCGGGACGCTGATCGTCAAGGCGGAAGCGCTTTTCCCACGCATCGAGGAGAA
GGAAGAAAAATAG

Upstream 100 bases:

>100_bases
GCGAACGTCAAGAGCGGGAGCAGGCTCCCCCCCGCGAGGAGGGGAACAACCGGGAGCGCCGGGGCGGCAAGGGAAGAGAC
CGGGATAAAAAGGAGAAGAA

Downstream 100 bases:

>100_bases
GTCCCTATCTGGCCGGAATCCTCAGCTCTGCCGACGCGAAGAGGAAGAAGAACCTGAACAGGCAGTACTTGTAGAACTCA
CTGAAGAGAAGCCGGAAGCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKKLKGYEVFFLTGTDEHGQKVEKAANTAGETPLELADRVVKRFQG
LWEKLDIQYTDFIRTTQERHKKGVSHIFTKIMAQGDIYLGEYEDWYCTPCETFWTETQLIDGKCPDCNRPVDKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNEKHVIYVWFDALTNYITALGYPD
EQSAFKTFWPVDVHLIGKDILRFHAVYWPTFLMAAGLPVPKKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDRYGIDAVRY
FLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLNRSTAMVNKYFDGVLPAPGPQTELDTAFREKTEAMVRQLDACMDE
LAFSKGLQAIWEVISAGNKYIDETAPWALAKDPAKREYLGTVMYTLLEAQRIVHLVLSAFMPRTSAKALGYLGWTEPCRE
EDLAWGRLAPGTLIVKAEALFPRIEEKEEK

Sequences:

>Translated_510_residues
MSRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKKLKGYEVFFLTGTDEHGQKVEKAANTAGETPLELADRVVKRFQG
LWEKLDIQYTDFIRTTQERHKKGVSHIFTKIMAQGDIYLGEYEDWYCTPCETFWTETQLIDGKCPDCNRPVDKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNEKHVIYVWFDALTNYITALGYPD
EQSAFKTFWPVDVHLIGKDILRFHAVYWPTFLMAAGLPVPKKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDRYGIDAVRY
FLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLNRSTAMVNKYFDGVLPAPGPQTELDTAFREKTEAMVRQLDACMDE
LAFSKGLQAIWEVISAGNKYIDETAPWALAKDPAKREYLGTVMYTLLEAQRIVHLVLSAFMPRTSAKALGYLGWTEPCRE
EDLAWGRLAPGTLIVKAEALFPRIEEKEEK
>Mature_509_residues
SRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKKLKGYEVFFLTGTDEHGQKVEKAANTAGETPLELADRVVKRFQGL
WEKLDIQYTDFIRTTQERHKKGVSHIFTKIMAQGDIYLGEYEDWYCTPCETFWTETQLIDGKCPDCNRPVDKLKEESYFF
RMSKYQEQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNEKHVIYVWFDALTNYITALGYPDE
QSAFKTFWPVDVHLIGKDILRFHAVYWPTFLMAAGLPVPKKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDRYGIDAVRYF
LLREVPFGLDGDFSHAALVHRINSDLANDLGNLLNRSTAMVNKYFDGVLPAPGPQTELDTAFREKTEAMVRQLDACMDEL
AFSKGLQAIWEVISAGNKYIDETAPWALAKDPAKREYLGTVMYTLLEAQRIVHLVLSAFMPRTSAKALGYLGWTEPCREE
DLAWGRLAPGTLIVKAEALFPRIEEKEEK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=38.9799635701275, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI14043022, Length=519, Percent_Identity=27.1676300578035, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1788432, Length=552, Percent_Identity=24.6376811594203, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI212645096, Length=522, Percent_Identity=34.8659003831418, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI71992781, Length=416, Percent_Identity=32.2115384615385, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17540794, Length=513, Percent_Identity=28.2651072124756, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321610, Length=518, Percent_Identity=34.7490347490347, Blast_Score=298, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=26.7985611510791, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21358483, Length=493, Percent_Identity=36.5111561866126, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24655311, Length=555, Percent_Identity=27.027027027027, Blast_Score=170, Evalue=3e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58389; Mature: 58258

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKKLKGYEVFFLTGTDEHGQKVEKAA
CCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
NTAGETPLELADRVVKRFQGLWEKLDIQYTDFIRTTQERHKKGVSHIFTKIMAQGDIYLG
HCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
EYEDWYCTPCETFWTETQLIDGKCPDCNRPVDKLKEESYFFRMSKYQEQLLAHIEANPDF
CCCCCEECCHHHHHCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNEKHVIYVWFDALTNYITALGYPD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
EQSAFKTFWPVDVHLIGKDILRFHAVYWPTFLMAAGLPVPKKVFAHGWWTVEGQKMSKSL
CCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLVDRYGIDAVRYFLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLNRSTA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVNKYFDGVLPAPGPQTELDTAFREKTEAMVRQLDACMDELAFSKGLQAIWEVISAGNKY
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IDETAPWALAKDPAKREYLGTVMYTLLEAQRIVHLVLSAFMPRTSAKALGYLGWTEPCRE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCH
EDLAWGRLAPGTLIVKAEALFPRIEEKEEK
HCCCCCCCCCCEEEEEEHHHCCCCHHCCCC
>Mature Secondary Structure 
SRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKKLKGYEVFFLTGTDEHGQKVEKAA
CCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
NTAGETPLELADRVVKRFQGLWEKLDIQYTDFIRTTQERHKKGVSHIFTKIMAQGDIYLG
HCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
EYEDWYCTPCETFWTETQLIDGKCPDCNRPVDKLKEESYFFRMSKYQEQLLAHIEANPDF
CCCCCEECCHHHHHCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNEKHVIYVWFDALTNYITALGYPD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
EQSAFKTFWPVDVHLIGKDILRFHAVYWPTFLMAAGLPVPKKVFAHGWWTVEGQKMSKSL
CCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLVDRYGIDAVRYFLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLNRSTA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVNKYFDGVLPAPGPQTELDTAFREKTEAMVRQLDACMDELAFSKGLQAIWEVISAGNKY
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IDETAPWALAKDPAKREYLGTVMYTLLEAQRIVHLVLSAFMPRTSAKALGYLGWTEPCRE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCH
EDLAWGRLAPGTLIVKAEALFPRIEEKEEK
HCCCCCCCCCCEEEEEEHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]