Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 39997105

GI number: 39997105

Start: 2200668

End: 2201741

Strand: Direct

Name: braE [H]

Synonym: GSU2007

Alternate gene names: 39997105

Gene position: 2200668-2201741 (Clockwise)

Preceding gene: 39997104

Following gene: 39997106

Centisome position: 57.7

GC content: 58.85

Gene sequence:

>1074_bases
ATGAGACAGATCAATGCTCTGATAACCAATACGCGGGGGGCCTCGCCGGTGTCGATCATCCTCGGGGTGGTCATCCTCGC
GGGCCTATTCCTCTTTCCCAAAGCAGTGGAGAGCCCCTACGCCCTTCACATCATGATCCTCTTCTTCCTCAGCACCATCA
TGGGGGAGAGCTGGAACATCGTCGGCGGCTACACGGGACAGTACTCAGTGGGCCATGCCGCCTATTTCGGGGCCGGCGCC
TACACCACCATGGTTCTGATGCAGTTCAAGCAGATCCCCCCTTGGTACGGGATGATTGCCGGCATCGCAGTGGCGCTGGT
GGTTTCCCTCATCATCGGCAGCATCTGCTTCCGACTCCGGGGACCCTATTTTGTTCTCGCGTCCATTGCCGTGGCCGAGA
TTATGCGCCTCTGCGCCATGAATCTGAAGGACCTGACCAACGGTGCCGAAGGTATCCTGGCCACAGACATCCCCCCTTTC
ACCCTGGGTGGGACGGTAATCACCGACTTTCTCTCCAAGGTCCCCTTCTACTACATCGGCCTGGTGATCGCCATCCTGAC
CATCGCAGTCACCTATCTGGTGCAGCACTCCAAACTCGGCTACTACTTCCAGGCCATCCGCGAAGACCAGGATGCGGCCC
ATTCTCTCGGGATCAACCTCTCTATCTACAAGAACATCGCCCTGGCCATCTCGGCGGTCTTCACGTCGCTGGCAGGCAGC
TTCTACGCCATCTACATCGGCTTTATCGACCCCCCTACAGTGCTCGCCCTGGACATCTCGGTTCAGATCGTTCTTATCTG
CATCATCGGCGGCATCGGCACCATCTGGGGACCGGTGGTGGGGTCACTGGTGCTGGTACCTCTCTCCGAAGCCCTGCGCA
GCAACCTGATCGCCCAGGGAATATTCAAGATCGGCCTGGCCAGCGAGGAGTCCGGAGTCGGGATCTTCCTCAAGGACCAC
CTGGCCCACGCCCACGCCCTGATCTACGGTATCCTGGTCGTAGTGGTCATCCTCTTCATGCCCGACGGCGTCCTGGGCTA
CGTCAAGAAACTCACCAAGAAAAAGGCGATGTGA

Upstream 100 bases:

>100_bases
CTGGAAGGACGTGGTCGTGTTCGTCCTCTTCCTCCTGGTGCTTCTCTTCAAGCCGGCGGGTCTGCTCGGCAAATCCAAAA
TGTAGCGGGGTCCGACTGAC

Downstream 100 bases:

>100_bases
GGTCAATCCCATGGCGATACTGGAAATAAAGCACGTCAGTAAATTCTTCGGCGGTCTCGCCGCTAACTCGGACGTCTCCT
TCTCCATGGAGGAGGGGATG

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRQINALITNTRGASPVSIILGVVILAGLFLFPKAVESPYALHIMILFFLSTIMGESWNIVGGYTGQYSVGHAAYFGAGA
YTTMVLMQFKQIPPWYGMIAGIAVALVVSLIIGSICFRLRGPYFVLASIAVAEIMRLCAMNLKDLTNGAEGILATDIPPF
TLGGTVITDFLSKVPFYYIGLVIAILTIAVTYLVQHSKLGYYFQAIREDQDAAHSLGINLSIYKNIALAISAVFTSLAGS
FYAIYIGFIDPPTVLALDISVQIVLICIIGGIGTIWGPVVGSLVLVPLSEALRSNLIAQGIFKIGLASEESGVGIFLKDH
LAHAHALIYGILVVVVILFMPDGVLGYVKKLTKKKAM

Sequences:

>Translated_357_residues
MRQINALITNTRGASPVSIILGVVILAGLFLFPKAVESPYALHIMILFFLSTIMGESWNIVGGYTGQYSVGHAAYFGAGA
YTTMVLMQFKQIPPWYGMIAGIAVALVVSLIIGSICFRLRGPYFVLASIAVAEIMRLCAMNLKDLTNGAEGILATDIPPF
TLGGTVITDFLSKVPFYYIGLVIAILTIAVTYLVQHSKLGYYFQAIREDQDAAHSLGINLSIYKNIALAISAVFTSLAGS
FYAIYIGFIDPPTVLALDISVQIVLICIIGGIGTIWGPVVGSLVLVPLSEALRSNLIAQGIFKIGLASEESGVGIFLKDH
LAHAHALIYGILVVVVILFMPDGVLGYVKKLTKKKAM
>Mature_357_residues
MRQINALITNTRGASPVSIILGVVILAGLFLFPKAVESPYALHIMILFFLSTIMGESWNIVGGYTGQYSVGHAAYFGAGA
YTTMVLMQFKQIPPWYGMIAGIAVALVVSLIIGSICFRLRGPYFVLASIAVAEIMRLCAMNLKDLTNGAEGILATDIPPF
TLGGTVITDFLSKVPFYYIGLVIAILTIAVTYLVQHSKLGYYFQAIREDQDAAHSLGINLSIYKNIALAISAVFTSLAGS
FYAIYIGFIDPPTVLALDISVQIVLICIIGGIGTIWGPVVGSLVLVPLSEALRSNLIAQGIFKIGLASEESGVGIFLKDH
LAHAHALIYGILVVVVILFMPDGVLGYVKKLTKKKAM

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=264, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38269; Mature: 38269

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQINALITNTRGASPVSIILGVVILAGLFLFPKAVESPYALHIMILFFLSTIMGESWNI
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
VGGYTGQYSVGHAAYFGAGAYTTMVLMQFKQIPPWYGMIAGIAVALVVSLIIGSICFRLR
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GPYFVLASIAVAEIMRLCAMNLKDLTNGAEGILATDIPPFTLGGTVITDFLSKVPFYYIG
CCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LVIAILTIAVTYLVQHSKLGYYFQAIREDQDAAHSLGINLSIYKNIALAISAVFTSLAGS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
FYAIYIGFIDPPTVLALDISVQIVLICIIGGIGTIWGPVVGSLVLVPLSEALRSNLIAQG
EEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFKIGLASEESGVGIFLKDHLAHAHALIYGILVVVVILFMPDGVLGYVKKLTKKKAM
HHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQINALITNTRGASPVSIILGVVILAGLFLFPKAVESPYALHIMILFFLSTIMGESWNI
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
VGGYTGQYSVGHAAYFGAGAYTTMVLMQFKQIPPWYGMIAGIAVALVVSLIIGSICFRLR
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GPYFVLASIAVAEIMRLCAMNLKDLTNGAEGILATDIPPFTLGGTVITDFLSKVPFYYIG
CCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LVIAILTIAVTYLVQHSKLGYYFQAIREDQDAAHSLGINLSIYKNIALAISAVFTSLAGS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
FYAIYIGFIDPPTVLALDISVQIVLICIIGGIGTIWGPVVGSLVLVPLSEALRSNLIAQG
EEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFKIGLASEESGVGIFLKDHLAHAHALIYGILVVVVILFMPDGVLGYVKKLTKKKAM
HHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]