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The map label for this gene is braD [H]

Identifier: 39997104

GI number: 39997104

Start: 2199786

End: 2200652

Strand: Direct

Name: braD [H]

Synonym: GSU2006

Alternate gene names: 39997104

Gene position: 2199786-2200652 (Clockwise)

Preceding gene: 39997103

Following gene: 39997105

Centisome position: 57.67

GC content: 58.13

Gene sequence:

>867_bases
ATGACCATTTTCCTCCAATCGCTCATCAGCGGCATTCTGATCGGCGGCGTCTACGCCCTGATCGGCATAGGACTCACCAT
CATCTTCGGTGTCATGCGGGTCATCAACTTCGCCCACGGCGACATCATGATGATCGGCATGTACCTGACCTATAACTTCT
TCTCCCTGGCGGGGGTCGATCCGTTCGTCTCCATCGCCCTCACCATCCCGCTCATGTTCCTCTTCGGAGCCATTCTCCAG
AAAACATTCATCAACCGCGTGCTCGGAGCCCTGCCCCAGAACCAAATACTGCTTACCATCGGCCTGGGACTGATTATGAG
CAACACGATGATGTTCATCTATACCTCCGACTACAAGATCCTGACTACATCTTATTCGTCGAGCAGCTTCAATATCGGCA
CCATCTCCATTTCATGGCCGCTGGCCATCTCTTTCCTGATCACCGCGGCGATAACGCTGCTCCTGTACTGGTTCCTGATG
AAGACCGACACGGGTCAGGCGATCCGGGCAACGGCCCAGGACCGGGAAGCGGCCCAGCTCATGGGGATCAACGTGAAGCG
GATGTCAATCCTGGCCTTCGGCATGGGTGCGGCCCTGGCCGGCACGGCTGGTGCGCTCATCTCCCCTACCTACTACATCT
TCCCCCAGATCGGGAGCACCTTCACCCTCAAGGCATTCGTCATCACGGTTCTCGGGGGAATGGGGAGCATCGTGGGGGCC
ACCATCGGCGGCGTCATCATCGGGGTGGCGGAGTCCCTCGGGGCCGTCTACATCTCCTCCGGCTGGAAGGACGTGGTCGT
GTTCGTCCTCTTCCTCCTGGTGCTTCTCTTCAAGCCGGCGGGTCTGCTCGGCAAATCCAAAATGTAG

Upstream 100 bases:

>100_bases
ACTGAGGCAGGTCAAGGGTCAAGGGCCGGAATGGAGGTTTTCCATCGTTCCGTCCCTTGACCCTTGATGCGTGAACCAGA
TTTTTTACCCGGAGCTATCC

Downstream 100 bases:

>100_bases
CGGGGTCCGACTGACATGAGACAGATCAATGCTCTGATAACCAATACGCGGGGGGCCTCGCCGGTGTCGATCATCCTCGG
GGTGGTCATCCTCGCGGGCC

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTIFLQSLISGILIGGVYALIGIGLTIIFGVMRVINFAHGDIMMIGMYLTYNFFSLAGVDPFVSIALTIPLMFLFGAILQ
KTFINRVLGALPQNQILLTIGLGLIMSNTMMFIYTSDYKILTTSYSSSSFNIGTISISWPLAISFLITAAITLLLYWFLM
KTDTGQAIRATAQDREAAQLMGINVKRMSILAFGMGAALAGTAGALISPTYYIFPQIGSTFTLKAFVITVLGGMGSIVGA
TIGGVIIGVAESLGAVYISSGWKDVVVFVLFLLVLLFKPAGLLGKSKM

Sequences:

>Translated_288_residues
MTIFLQSLISGILIGGVYALIGIGLTIIFGVMRVINFAHGDIMMIGMYLTYNFFSLAGVDPFVSIALTIPLMFLFGAILQ
KTFINRVLGALPQNQILLTIGLGLIMSNTMMFIYTSDYKILTTSYSSSSFNIGTISISWPLAISFLITAAITLLLYWFLM
KTDTGQAIRATAQDREAAQLMGINVKRMSILAFGMGAALAGTAGALISPTYYIFPQIGSTFTLKAFVITVLGGMGSIVGA
TIGGVIIGVAESLGAVYISSGWKDVVVFVLFLLVLLFKPAGLLGKSKM
>Mature_287_residues
TIFLQSLISGILIGGVYALIGIGLTIIFGVMRVINFAHGDIMMIGMYLTYNFFSLAGVDPFVSIALTIPLMFLFGAILQK
TFINRVLGALPQNQILLTIGLGLIMSNTMMFIYTSDYKILTTSYSSSSFNIGTISISWPLAISFLITAAITLLLYWFLMK
TDTGQAIRATAQDREAAQLMGINVKRMSILAFGMGAALAGTAGALISPTYYIFPQIGSTFTLKAFVITVLGGMGSIVGAT
IGGVIIGVAESLGAVYISSGWKDVVVFVLFLLVLLFKPAGLLGKSKM

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=303, Percent_Identity=34.983498349835, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 30772; Mature: 30640

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIFLQSLISGILIGGVYALIGIGLTIIFGVMRVINFAHGDIMMIGMYLTYNFFSLAGVD
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
PFVSIALTIPLMFLFGAILQKTFINRVLGALPQNQILLTIGLGLIMSNTMMFIYTSDYKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCEEEEEECCEEE
LTTSYSSSSFNIGTISISWPLAISFLITAAITLLLYWFLMKTDTGQAIRATAQDREAAQL
EEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MGINVKRMSILAFGMGAALAGTAGALISPTYYIFPQIGSTFTLKAFVITVLGGMGSIVGA
HCCCHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TIGGVIIGVAESLGAVYISSGWKDVVVFVLFLLVLLFKPAGLLGKSKM
HHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TIFLQSLISGILIGGVYALIGIGLTIIFGVMRVINFAHGDIMMIGMYLTYNFFSLAGVD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
PFVSIALTIPLMFLFGAILQKTFINRVLGALPQNQILLTIGLGLIMSNTMMFIYTSDYKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCEEEEEECCEEE
LTTSYSSSSFNIGTISISWPLAISFLITAAITLLLYWFLMKTDTGQAIRATAQDREAAQL
EEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MGINVKRMSILAFGMGAALAGTAGALISPTYYIFPQIGSTFTLKAFVITVLGGMGSIVGA
HCCCHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TIGGVIIGVAESLGAVYISSGWKDVVVFVLFLLVLLFKPAGLLGKSKM
HHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]