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The map label for this gene is purN [H]

Identifier: 39996858

GI number: 39996858

Start: 1921478

End: 1922098

Strand: Direct

Name: purN [H]

Synonym: GSU1759

Alternate gene names: 39996858

Gene position: 1921478-1922098 (Clockwise)

Preceding gene: 39996857

Following gene: 39996859

Centisome position: 50.38

GC content: 59.58

Gene sequence:

>621_bases
ATGATTCAACCGCTTGCAGTTGGTGTTCTCGTTTCGGGCAATGGCTCGAATCTCCAGGCGATCATCGACCGGATCGAGGA
TGGCTCGCTCCCGGCGAGAATAGTCTGCGTGATCAGCAACAAAGCTGATGCCTTTGGCCTTGAGCGCGCTAGAAAGCACG
GCGTACCCGCCATCCATATCGATCACAGGGCCCACGGCGGCCGTGAATCCTACGATGCGGCCCTAGTGGAAACCCTGCGC
TCCCACGGGGTGCAACTCGTCGTCCTGGCAGGCTTCATGCGCATCGTTACGCCGGTGTTGCTTGACGCCTTCCCCAATGC
GGTCATGAATATCCATCCCGCCCTCCTGCCCGCCTTTCCCGGGCTCCACGCCCAGGCTCAGGCGCTCCGGTACGGAGTCA
AGTTCTCCGGTTGCACTGTCCACTTTGTTGATGAAGGGACCGACACAGGACCGATCATCATCCAGGCAGCCGTCCCGGTC
ATGGATGATGACGATGAAGCCTCCCTTTCGGCCCGTATCCAGAGAGAAGAGCACCGGGCCTATCCCGAGGCTATCCGGCT
GTTTGCCGCGAAACGCCTCAGGATCGAAGGAAGAAAGGTATCCATACTTCAGGGGGCATGA

Upstream 100 bases:

>100_bases
TCAGGAGAAGGCCTTCATCATCGGCGAAATCGCCAAGTGCGAGCCCGGCGCCGAGATGGTCGAACTCGTATAAACGGGAC
GTGGCACCATGGGAGCCCCT

Downstream 100 bases:

>100_bases
CCACGCAACATGCTCTCCGGTTATTCCGCGATCGAGGAACGGTGCCATGAAACGAACGGTCATTTTATTCGCTGCCATGA
TTCTAACCGCCTCTGTCGGC

Product: phosphoribosylformylglycinamidine synthase II

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MIQPLAVGVLVSGNGSNLQAIIDRIEDGSLPARIVCVISNKADAFGLERARKHGVPAIHIDHRAHGGRESYDAALVETLR
SHGVQLVVLAGFMRIVTPVLLDAFPNAVMNIHPALLPAFPGLHAQAQALRYGVKFSGCTVHFVDEGTDTGPIIIQAAVPV
MDDDDEASLSARIQREEHRAYPEAIRLFAAKRLRIEGRKVSILQGA

Sequences:

>Translated_206_residues
MIQPLAVGVLVSGNGSNLQAIIDRIEDGSLPARIVCVISNKADAFGLERARKHGVPAIHIDHRAHGGRESYDAALVETLR
SHGVQLVVLAGFMRIVTPVLLDAFPNAVMNIHPALLPAFPGLHAQAQALRYGVKFSGCTVHFVDEGTDTGPIIIQAAVPV
MDDDDEASLSARIQREEHRAYPEAIRLFAAKRLRIEGRKVSILQGA
>Mature_206_residues
MIQPLAVGVLVSGNGSNLQAIIDRIEDGSLPARIVCVISNKADAFGLERARKHGVPAIHIDHRAHGGRESYDAALVETLR
SHGVQLVVLAGFMRIVTPVLLDAFPNAVMNIHPALLPAFPGLHAQAQALRYGVKFSGCTVHFVDEGTDTGPIIIQAAVPV
MDDDDEASLSARIQREEHRAYPEAIRLFAAKRLRIEGRKVSILQGA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=43.9153439153439, Blast_Score=165, Evalue=2e-41,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=43.9153439153439, Blast_Score=165, Evalue=2e-41,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=43.9153439153439, Blast_Score=165, Evalue=2e-41,
Organism=Escherichia coli, GI1788846, Length=187, Percent_Identity=47.0588235294118, Blast_Score=176, Evalue=8e-46,
Organism=Escherichia coli, GI1787483, Length=196, Percent_Identity=30.6122448979592, Blast_Score=85, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=42.7807486631016, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=30.1507537688442, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=51.6304347826087, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22202; Mature: 22202

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPLAVGVLVSGNGSNLQAIIDRIEDGSLPARIVCVISNKADAFGLERARKHGVPAIHI
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEE
DHRAHGGRESYDAALVETLRSHGVQLVVLAGFMRIVTPVLLDAFPNAVMNIHPALLPAFP
CCCCCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHCCC
GLHAQAQALRYGVKFSGCTVHFVDEGTDTGPIIIQAAVPVMDDDDEASLSARIQREEHRA
CCCHHHHHHHHCCEECCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHC
YPEAIRLFAAKRLRIEGRKVSILQGA
HHHHHHHHHHHHHEECCCEEEEEECC
>Mature Secondary Structure
MIQPLAVGVLVSGNGSNLQAIIDRIEDGSLPARIVCVISNKADAFGLERARKHGVPAIHI
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEE
DHRAHGGRESYDAALVETLRSHGVQLVVLAGFMRIVTPVLLDAFPNAVMNIHPALLPAFP
CCCCCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHCCC
GLHAQAQALRYGVKFSGCTVHFVDEGTDTGPIIIQAAVPVMDDDDEASLSARIQREEHRA
CCCHHHHHHHHCCEECCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHC
YPEAIRLFAAKRLRIEGRKVSILQGA
HHHHHHHHHHHHHEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]