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The map label for this gene is recG [H]

Identifier: 39996428

GI number: 39996428

Start: 1450443

End: 1452587

Strand: Direct

Name: recG [H]

Synonym: GSU1326

Alternate gene names: 39996428

Gene position: 1450443-1452587 (Clockwise)

Preceding gene: 39996425

Following gene: 39996429

Centisome position: 38.03

GC content: 68.25

Gene sequence:

>2145_bases
GTGAGCCTCCGGCAGGCCATTGCGCGCAAGAACCTTGAGACCCCCATGCAGTTCATCAAGGGGGTGGGGCCGAAGATGGC
CGAGATCCTGGCGCGGAAGGGGATCGTCACCGTGGAGGATGCGCTCTACCTGCTCCCGAACCGCTACGAGGACCGCCGCC
AAGTGGTGCCCATGAACCGGCTCCGCCCCGGTGAGACCTGCGTGTTTGCAGGGGAGGTGGTGTCGGCCGACGTGGTGACC
ACCAAGGGGGGGCGCCGCTTCTTCGAGGTGATGGCGCGGGATGCGAGCGGTGTCATGTCCTTCAAGTGGTTCAACTACCA
TGCGGCCTTCATGCGCAAGGCCTGGCAGCCGGGCCGGCGGGGAATCTTCACCGGCCAGGTGACCCAGTACGGTCTCCAGC
GTGAGGTACATCATCCGGACGTTGACTGGCTGGCCGAGGGGGAGACGGTGGAGGCGGCCATGGCGCGGGACCCGGTGGGA
TTCGGCCGGCTGGTGCCGGTCTATCCGCTCACCGAGGGGCTGAACCAGAAGGCGCTGCGCAAGGTCATGAAGGAGGTGGT
GGACCGCTTCGCCCCCTGCGTGGAGAGCGCCCTGCCGGCCCGGGTGATCCGTGAGCGGGCGCTGCTGCCCCTGGCCGAGG
CCCTGGGAGCGGTCCATTTCCCGGCTGCCGACGCCGATCCGCGTGCCCTGGAGGAGGGGCGGGACCCGGCCCACCGGACC
CTGGTTTTCGACGAATTCTTCTTCCTGGAGCTGGGGCTGGCGCTCAAGCGGCGGGGAGTGACCCTGGAACCGGGAATCGC
GTTTTCCGTGAACCACACATACACCAAGCCGCTCCTGAAGCTGCTTCCCTTTGCCCTGACAGAGGCCCAGCGCCGGGTCC
TGGCCGAGATCAAGGCCGACATGATGGCGCCCCACCCCATGCACCGGCTGGTGCAGGGGGACGTGGGCTGCGGCAAGACC
CTGGTGGCGCTCCTGGCGGCGCTGGTGGCGGTGGAGAACGGCTACCAGGTGGCCATCATGGCTCCCACCGAGATCCTGGC
CGAGCAGCACTACCTGAACATCCACCGCTGGTGCGAGCAGTTGGGGGTGACCGTGGCGCTCCTCACGTCGTCCCTGAAGG
GAAAGGCCAAGGCCGGAGCCCTGGAGCGGGTGGCGCGGGGCGAGGTGCAGATCGTGATCGGCACCCACGCAGTGATCCAG
GACAAGGTGGAGTTCCACCGGCTGGGGCTCGGCATCGTGGACGAGCAGCACCGTTTCGGCGTGCTCCAGCGGGGGCTCCT
GCGCAAGAAGGGGGAAAACCCGGACATCCTCGTGATGACCGCCACCCCGATCCCGCGCACCCTGGCCATGACCGTGTTCG
GCGACCTGTCCCTGTCGGTCATCGACGAGCTCCCGCCGGGCCGCACCCCGGTGGAGACGAAGATCTGCACCGATTCCCGC
CGCCGGCAGGTCTACGGCATCATCCGCGACGAAGTGGCCGCGGGGCACCAGGCCTACGTCATCTACCCCCTGGTGGAGGA
GTCGGAGAAGAGCGAGCTCAAGGCGGCCACCCAGATGGCCGAACACCTGGCCAATGACGTGTTCCCGGATCTGCGCCTCG
GTGTTCTCCACGGCCGGATGAAGCCCGAGGAGAAGGAGGCGGTGATGCGCTCCTTCAAGGGACGGGAGATCGACATCCTG
GTTTCCACCACGGTCATCGAGGTGGGGATTGACGTGCCCAACGCCACGGTGATGGTGATCGAGCACGCGGAGCGGTTCGG
CCTTTCCCAGCTCCACCAGCTGCGGGGGAGGGTGGGGCGCGGCAGTGCCACGTCCCGCTGCATCCTCATGGCGTCGGACC
GGTTGTCCGATGACGGGGTCAAGCGGCTGCGGGTGATGGAATCGACCACCGACGGCTTCAGGATCGCCGAGGCTGATCTG
GAGATCAGGGGGCCGGGCGATTTCCTCGGCACCCGCCAGGCGGGCATCCCCGATTTTCGCGTGGCCAACATCCTGCGGGA
CGGCCGCATCCTGGAAGAGGCCCGGGCCGAGGCTTTCGATCTGGCGGAGCGGGACCCTGAGTTGCGGGCCCCCGGCCACG
AACCGCTCCGGGAGGAGCTGGCGCGGCGCTGGGGCGGCCGGCTGGAACTGGCCGGGGTGGCCTGA

Upstream 100 bases:

>100_bases
ACCCCTCGGACTTGCGCCCGGCGACCGGGTGGCGGTGGAGGCGGACGCAGAGGGGCGCCTGGTGCTGACCCGGTCCGCGG
CCCGGGAGCCATCGGTTGAG

Downstream 100 bases:

>100_bases
GGAGCGAGACCATGACTGACTATGTATCGTTTTCCGCGTTTCTGGCGGAGTCCCCCGTGATCCTCGGCGAAGGGGCGGTT
ATCGAGCGCCTGCGCCGGGC

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSLRQAIARKNLETPMQFIKGVGPKMAEILARKGIVTVEDALYLLPNRYEDRRQVVPMNRLRPGETCVFAGEVVSADVVT
TKGGRRFFEVMARDASGVMSFKWFNYHAAFMRKAWQPGRRGIFTGQVTQYGLQREVHHPDVDWLAEGETVEAAMARDPVG
FGRLVPVYPLTEGLNQKALRKVMKEVVDRFAPCVESALPARVIRERALLPLAEALGAVHFPAADADPRALEEGRDPAHRT
LVFDEFFFLELGLALKRRGVTLEPGIAFSVNHTYTKPLLKLLPFALTEAQRRVLAEIKADMMAPHPMHRLVQGDVGCGKT
LVALLAALVAVENGYQVAIMAPTEILAEQHYLNIHRWCEQLGVTVALLTSSLKGKAKAGALERVARGEVQIVIGTHAVIQ
DKVEFHRLGLGIVDEQHRFGVLQRGLLRKKGENPDILVMTATPIPRTLAMTVFGDLSLSVIDELPPGRTPVETKICTDSR
RRQVYGIIRDEVAAGHQAYVIYPLVEESEKSELKAATQMAEHLANDVFPDLRLGVLHGRMKPEEKEAVMRSFKGREIDIL
VSTTVIEVGIDVPNATVMVIEHAERFGLSQLHQLRGRVGRGSATSRCILMASDRLSDDGVKRLRVMESTTDGFRIAEADL
EIRGPGDFLGTRQAGIPDFRVANILRDGRILEEARAEAFDLAERDPELRAPGHEPLREELARRWGGRLELAGVA

Sequences:

>Translated_714_residues
MSLRQAIARKNLETPMQFIKGVGPKMAEILARKGIVTVEDALYLLPNRYEDRRQVVPMNRLRPGETCVFAGEVVSADVVT
TKGGRRFFEVMARDASGVMSFKWFNYHAAFMRKAWQPGRRGIFTGQVTQYGLQREVHHPDVDWLAEGETVEAAMARDPVG
FGRLVPVYPLTEGLNQKALRKVMKEVVDRFAPCVESALPARVIRERALLPLAEALGAVHFPAADADPRALEEGRDPAHRT
LVFDEFFFLELGLALKRRGVTLEPGIAFSVNHTYTKPLLKLLPFALTEAQRRVLAEIKADMMAPHPMHRLVQGDVGCGKT
LVALLAALVAVENGYQVAIMAPTEILAEQHYLNIHRWCEQLGVTVALLTSSLKGKAKAGALERVARGEVQIVIGTHAVIQ
DKVEFHRLGLGIVDEQHRFGVLQRGLLRKKGENPDILVMTATPIPRTLAMTVFGDLSLSVIDELPPGRTPVETKICTDSR
RRQVYGIIRDEVAAGHQAYVIYPLVEESEKSELKAATQMAEHLANDVFPDLRLGVLHGRMKPEEKEAVMRSFKGREIDIL
VSTTVIEVGIDVPNATVMVIEHAERFGLSQLHQLRGRVGRGSATSRCILMASDRLSDDGVKRLRVMESTTDGFRIAEADL
EIRGPGDFLGTRQAGIPDFRVANILRDGRILEEARAEAFDLAERDPELRAPGHEPLREELARRWGGRLELAGVA
>Mature_713_residues
SLRQAIARKNLETPMQFIKGVGPKMAEILARKGIVTVEDALYLLPNRYEDRRQVVPMNRLRPGETCVFAGEVVSADVVTT
KGGRRFFEVMARDASGVMSFKWFNYHAAFMRKAWQPGRRGIFTGQVTQYGLQREVHHPDVDWLAEGETVEAAMARDPVGF
GRLVPVYPLTEGLNQKALRKVMKEVVDRFAPCVESALPARVIRERALLPLAEALGAVHFPAADADPRALEEGRDPAHRTL
VFDEFFFLELGLALKRRGVTLEPGIAFSVNHTYTKPLLKLLPFALTEAQRRVLAEIKADMMAPHPMHRLVQGDVGCGKTL
VALLAALVAVENGYQVAIMAPTEILAEQHYLNIHRWCEQLGVTVALLTSSLKGKAKAGALERVARGEVQIVIGTHAVIQD
KVEFHRLGLGIVDEQHRFGVLQRGLLRKKGENPDILVMTATPIPRTLAMTVFGDLSLSVIDELPPGRTPVETKICTDSRR
RQVYGIIRDEVAAGHQAYVIYPLVEESEKSELKAATQMAEHLANDVFPDLRLGVLHGRMKPEEKEAVMRSFKGREIDILV
STTVIEVGIDVPNATVMVIEHAERFGLSQLHQLRGRVGRGSATSRCILMASDRLSDDGVKRLRVMESTTDGFRIAEADLE
IRGPGDFLGTRQAGIPDFRVANILRDGRILEEARAEAFDLAERDPELRAPGHEPLREELARRWGGRLELAGVA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=679, Percent_Identity=45.0662739322533, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1787357, Length=396, Percent_Identity=40.4040404040404, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79247; Mature: 79116

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRQAIARKNLETPMQFIKGVGPKMAEILARKGIVTVEDALYLLPNRYEDRRQVVPMNR
CCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCEEECHHHHCCCCCCCCHHHCCCHHC
LRPGETCVFAGEVVSADVVTTKGGRRFFEVMARDASGVMSFKWFNYHAAFMRKAWQPGRR
CCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCC
GIFTGQVTQYGLQREVHHPDVDWLAEGETVEAAMARDPVGFGRLVPVYPLTEGLNQKALR
CEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHH
KVMKEVVDRFAPCVESALPARVIRERALLPLAEALGAVHFPAADADPRALEEGRDPAHRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHCCCCCHHHE
LVFDEFFFLELGLALKRRGVTLEPGIAFSVNHTYTKPLLKLLPFALTEAQRRVLAEIKAD
EEHHHHHHHHHHHHHHHCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMAPHPMHRLVQGDVGCGKTLVALLAALVAVENGYQVAIMAPTEILAEQHYLNIHRWCEQ
HCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHH
LGVTVALLTSSLKGKAKAGALERVARGEVQIVIGTHAVIQDKVEFHRLGLGIVDEQHRFG
HCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCEECCHHHHH
VLQRGLLRKKGENPDILVMTATPIPRTLAMTVFGDLSLSVIDELPPGRTPVETKICTDSR
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEECCCCHH
RRQVYGIIRDEVAAGHQAYVIYPLVEESEKSELKAATQMAEHLANDVFPDLRLGVLHGRM
HHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
KPEEKEAVMRSFKGREIDILVSTTVIEVGIDVPNATVMVIEHAERFGLSQLHQLRGRVGR
CCHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCEEEEEECHHHCCHHHHHHHHCCCCC
GSATSRCILMASDRLSDDGVKRLRVMESTTDGFRIAEADLEIRGPGDFLGTRQAGIPDFR
CCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCEEEECCCCCCCCCCCCCCCHH
VANILRDGRILEEARAEAFDLAERDPELRAPGHEPLREELARRWGGRLELAGVA
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
SLRQAIARKNLETPMQFIKGVGPKMAEILARKGIVTVEDALYLLPNRYEDRRQVVPMNR
CHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCEEECHHHHCCCCCCCCHHHCCCHHC
LRPGETCVFAGEVVSADVVTTKGGRRFFEVMARDASGVMSFKWFNYHAAFMRKAWQPGRR
CCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCC
GIFTGQVTQYGLQREVHHPDVDWLAEGETVEAAMARDPVGFGRLVPVYPLTEGLNQKALR
CEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHH
KVMKEVVDRFAPCVESALPARVIRERALLPLAEALGAVHFPAADADPRALEEGRDPAHRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHCCCCCHHHE
LVFDEFFFLELGLALKRRGVTLEPGIAFSVNHTYTKPLLKLLPFALTEAQRRVLAEIKAD
EEHHHHHHHHHHHHHHHCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMAPHPMHRLVQGDVGCGKTLVALLAALVAVENGYQVAIMAPTEILAEQHYLNIHRWCEQ
HCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHH
LGVTVALLTSSLKGKAKAGALERVARGEVQIVIGTHAVIQDKVEFHRLGLGIVDEQHRFG
HCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCEECCHHHHH
VLQRGLLRKKGENPDILVMTATPIPRTLAMTVFGDLSLSVIDELPPGRTPVETKICTDSR
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEECCCCHH
RRQVYGIIRDEVAAGHQAYVIYPLVEESEKSELKAATQMAEHLANDVFPDLRLGVLHGRM
HHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
KPEEKEAVMRSFKGREIDILVSTTVIEVGIDVPNATVMVIEHAERFGLSQLHQLRGRVGR
CCHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCEEEEEECHHHCCHHHHHHHHCCCCC
GSATSRCILMASDRLSDDGVKRLRVMESTTDGFRIAEADLEIRGPGDFLGTRQAGIPDFR
CCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCEEEECCCCCCCCCCCCCCCHH
VANILRDGRILEEARAEAFDLAERDPELRAPGHEPLREELARRWGGRLELAGVA
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]