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The map label for this gene is truD

Identifier: 39995627

GI number: 39995627

Start: 553701

End: 554927

Strand: Reverse

Name: truD

Synonym: GSU0520

Alternate gene names: 39995627

Gene position: 554927-553701 (Counterclockwise)

Preceding gene: 39995628

Following gene: 39995626

Centisome position: 14.55

GC content: 68.87

Gene sequence:

>1227_bases
GTGGCGCGCGATGAGCATGACAGCCGTTACCTGACGGCAGAGCTTCCCGGCACGGGCGGCCTCTTCAAGGAAACGCCCGA
GGATTTCCTGGTTGAGGAGATCTCTCTCTACCTCCCCTGCGGCGAGGGTGAGCACACCTACGCAGTGATCGAAAAGCGGG
GTATCACCACTCTGGAGGCCATCAGGCGCCTCTGCCGCGCCACGGGCGCCCCCGAGCGGGACACGGGCTATGCCGGCATG
AAGGACGCCAGGGGGATAACCCGGCAGACGGTTTCCCTGCCGCGGGTGACACCGGCAGAGGTGATGGGGCTCAACATACC
GGGCATCCGAATCCTGTCCGCGGACCGGCATCGGAACAAGCTGCGCTTGGGCCACTTGGCCGGCAACCGCTTCCGGCTCC
GGCTCCGGGAGACGGTCCCCGACGCGGCCGACCGGGCACGCGCCATCCTCGACGTTCTCGCCCGGCGCGGGGTCCCCAAC
CGCTTCGGCGAGCAGCGCTACGGCATCCAGGGAAACAGCCACCTGGTGGGCCGGGCCATGCTGGCCGGCGACTGGCGCGG
AGCTGTGGACCTCCTGATGGGCGACCCGGCAAAGGTGGAGGGCGAACGGTGGCGGGCCGCCATCGAGGCCTACCGACAGG
GCGACCTGGCCGGGAGCCTCAGCCTCTTTCCCGGACACTGCCGGACCGAGCGGGACGTGCTCCAGCGCCTGGCAAAGCGC
CCGGACGACTTTGAGCGCGCCGTCCACGGGGTCCACCCCCGGCTCAAGAAACTCTACCTCTCGGCCTGCCAGTCGGCCCT
CTTCGACCGGGTTCTGGAGACCCGGCTCCAGACCATCGACCACGTGATGGAGGGTGACCTGGCCTGGAAACACGATAACG
GCGCCTGCTTCCTGGTGACCGATGCCAAGGCCGAGGCACCCCGGGCCGAGCGATTCGAGATATCCCCCACCGGTCCCCTC
TTCGGCTGTCGCATGACCTCTCCCGAAGGGGAACCGGCGGCCCTGGAAAAGAGCATTCTCGATGCGGCGGGACTCACGCC
GGCAGCCTTCAACCTGGCCGGCGGGCTGCGGATGGAGGGGGAGCGCCGCCCCCTGAGGGTTCCCCTGGAGGGACCCGAGC
TATCCTGCGAGGAGGAAGACCTGGTGCTCCGGTTCTCGCTGCCCCGGGGAAGCTATGCCACGGCAGTGCTGCGGGAGGTC
ATGAAGCGCCCGGGAAACGGCGCCTGA

Upstream 100 bases:

>100_bases
ACTGGAAGGCTATCACCTCTCCAAATACATGCAGAAGTTCATGGCCGAGGGAAAACGGATCTACTTCATCCACTACCGCA
CGACAATGGAGGGGTGACGC

Downstream 100 bases:

>100_bases
CAGGAGACCTATTAATGATACAATGCATTGTGATGCGCGTGGCCGGCTACAGCCCGCGCCCGGGAGGGCACAGCCCATGA
ACGATCCGCAAAAACTTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MARDEHDSRYLTAELPGTGGLFKETPEDFLVEEISLYLPCGEGEHTYAVIEKRGITTLEAIRRLCRATGAPERDTGYAGM
KDARGITRQTVSLPRVTPAEVMGLNIPGIRILSADRHRNKLRLGHLAGNRFRLRLRETVPDAADRARAILDVLARRGVPN
RFGEQRYGIQGNSHLVGRAMLAGDWRGAVDLLMGDPAKVEGERWRAAIEAYRQGDLAGSLSLFPGHCRTERDVLQRLAKR
PDDFERAVHGVHPRLKKLYLSACQSALFDRVLETRLQTIDHVMEGDLAWKHDNGACFLVTDAKAEAPRAERFEISPTGPL
FGCRMTSPEGEPAALEKSILDAAGLTPAAFNLAGGLRMEGERRPLRVPLEGPELSCEEEDLVLRFSLPRGSYATAVLREV
MKRPGNGA

Sequences:

>Translated_408_residues
MARDEHDSRYLTAELPGTGGLFKETPEDFLVEEISLYLPCGEGEHTYAVIEKRGITTLEAIRRLCRATGAPERDTGYAGM
KDARGITRQTVSLPRVTPAEVMGLNIPGIRILSADRHRNKLRLGHLAGNRFRLRLRETVPDAADRARAILDVLARRGVPN
RFGEQRYGIQGNSHLVGRAMLAGDWRGAVDLLMGDPAKVEGERWRAAIEAYRQGDLAGSLSLFPGHCRTERDVLQRLAKR
PDDFERAVHGVHPRLKKLYLSACQSALFDRVLETRLQTIDHVMEGDLAWKHDNGACFLVTDAKAEAPRAERFEISPTGPL
FGCRMTSPEGEPAALEKSILDAAGLTPAAFNLAGGLRMEGERRPLRVPLEGPELSCEEEDLVLRFSLPRGSYATAVLREV
MKRPGNGA
>Mature_407_residues
ARDEHDSRYLTAELPGTGGLFKETPEDFLVEEISLYLPCGEGEHTYAVIEKRGITTLEAIRRLCRATGAPERDTGYAGMK
DARGITRQTVSLPRVTPAEVMGLNIPGIRILSADRHRNKLRLGHLAGNRFRLRLRETVPDAADRARAILDVLARRGVPNR
FGEQRYGIQGNSHLVGRAMLAGDWRGAVDLLMGDPAKVEGERWRAAIEAYRQGDLAGSLSLFPGHCRTERDVLQRLAKRP
DDFERAVHGVHPRLKKLYLSACQSALFDRVLETRLQTIDHVMEGDLAWKHDNGACFLVTDAKAEAPRAERFEISPTGPLF
GCRMTSPEGEPAALEKSILDAAGLTPAAFNLAGGLRMEGERRPLRVPLEGPELSCEEEDLVLRFSLPRGSYATAVLREVM
KRPGNGA

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI148762985, Length=413, Percent_Identity=25.181598062954, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148762961, Length=413, Percent_Identity=25.181598062954, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148762948, Length=413, Percent_Identity=25.181598062954, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI50727002, Length=405, Percent_Identity=24.9382716049383, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789102, Length=170, Percent_Identity=38.8235294117647, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17557232, Length=358, Percent_Identity=24.5810055865922, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324817, Length=251, Percent_Identity=25.0996015936255, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355799, Length=449, Percent_Identity=23.6080178173719, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_GEOSL (P60347)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951578.1
- ProteinModelPortal:   P60347
- SMR:   P60347
- GeneID:   2686060
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0520
- NMPDR:   fig|243231.1.peg.516
- TIGR:   GSU0520
- HOGENOM:   HBG541221
- OMA:   WAGTKDK
- ProtClustDB:   CLSK827897
- BioCyc:   GSUL243231:GSU_0520-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 45100; Mature: 44969

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 82-82

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDEHDSRYLTAELPGTGGLFKETPEDFLVEEISLYLPCGEGEHTYAVIEKRGITTLEA
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHEEEECCCCCCEEEEEECCCCHHHHH
IRRLCRATGAPERDTGYAGMKDARGITRQTVSLPRVTPAEVMGLNIPGIRILSADRHRNK
HHHHHHHCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCHHHHEECCCCCEEEEECCCCCCC
LRLGHLAGNRFRLRLRETVPDAADRARAILDVLARRGVPNRFGEQRYGIQGNSHLVGRAM
EEEEEECCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEHHH
LAGDWRGAVDLLMGDPAKVEGERWRAAIEAYRQGDLAGSLSLFPGHCRTERDVLQRLAKR
ECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PDDFERAVHGVHPRLKKLYLSACQSALFDRVLETRLQTIDHVMEGDLAWKHDNGACFLVT
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEE
DAKAEAPRAERFEISPTGPLFGCRMTSPEGEPAALEKSILDAAGLTPAAFNLAGGLRMEG
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEECC
ERRPLRVPLEGPELSCEEEDLVLRFSLPRGSYATAVLREVMKRPGNGA
CCCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARDEHDSRYLTAELPGTGGLFKETPEDFLVEEISLYLPCGEGEHTYAVIEKRGITTLEA
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHEEEECCCCCCEEEEEECCCCHHHHH
IRRLCRATGAPERDTGYAGMKDARGITRQTVSLPRVTPAEVMGLNIPGIRILSADRHRNK
HHHHHHHCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCHHHHEECCCCCEEEEECCCCCCC
LRLGHLAGNRFRLRLRETVPDAADRARAILDVLARRGVPNRFGEQRYGIQGNSHLVGRAM
EEEEEECCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEHHH
LAGDWRGAVDLLMGDPAKVEGERWRAAIEAYRQGDLAGSLSLFPGHCRTERDVLQRLAKR
ECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PDDFERAVHGVHPRLKKLYLSACQSALFDRVLETRLQTIDHVMEGDLAWKHDNGACFLVT
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEE
DAKAEAPRAERFEISPTGPLFGCRMTSPEGEPAALEKSILDAAGLTPAAFNLAGGLRMEG
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEECC
ERRPLRVPLEGPELSCEEEDLVLRFSLPRGSYATAVLREVMKRPGNGA
CCCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA