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The map label for this gene is glnG [H]

Identifier: 39995390

GI number: 39995390

Start: 307916

End: 309268

Strand: Reverse

Name: glnG [H]

Synonym: GSU0280

Alternate gene names: 39995390

Gene position: 309268-307916 (Counterclockwise)

Preceding gene: 39995392

Following gene: 39995389

Centisome position: 8.11

GC content: 58.31

Gene sequence:

>1353_bases
ATGGGCGAACTGACCGATGTGACAATCCTGTATGTTGAAGATGAGCCGGTGCTGAGGGAACAGGTCGTGTTCGCTCTGCG
CATGCATTTTTCACGAGTATTGAGTGCCTGTGACGGGAAAGAGGCCATGCGCATGATCAGGGCTGAGTGCCCCGACGTGG
TAGTCAGCGATATCCGGATGCCGGCAATGGACGGCTTGACCCTTGCATCGCTGCTCAAGACCGAACTTCCCGATCTCCCC
GTTGTCCTCTGCACAGCCCTCCACGAAACAGACCATCTTCTGAAAGCTATCGAATTGGGAGTTGCGGCCTATGTCAGCAA
GCCGATCGATCTGGAGAAACTGCAACATGCCATCAGACTGGCGAGCCTTCCCTGCGTCAACCGGCGAGAAATAGCCCGTC
TGCGCTCCGAGGCCGCAATCTCTAACGGCCTGCTGGGAGAAGGCTCCATCATGCGTCCCATTGCCGAACAACTGCAGCGG
GTTGCCGATACAGACCTTTCAGTGATGCTCCTCGGAGAGCCCGGAACAGGCAAAAACTTTCTGGCCGCAAAAGTTCATGC
CTTGAGCAAACGCAAAACCCAACCGTTTCTTGCGGTCGACTGCCTGGCACGAACTCCGGAGCAGTTGGAGAAGGAGCTCT
TTGGCGAAGCCAAGGGACGGGGCAGACCATCCTCCGGCGCCACGGAGCTGCTTTCCGAGGCGGATGGAGGCACGCTGCTG
CTTGACGGGCCGGAACGACTGCCGTTGCAACTGCAGCAAAGACTGTTCAATCTGCTCGAAGACGGCATGTTCGTGCCAAA
TGGTTCCATCAGGCCGGTTGCGTGCAATCTGCGCGTGATCACGGTTACCGGCGCCAATCTGGATAAACTGGCGCAGGCAG
GAACGTTCAGCCAGGAGCTGCTCGAGCGGCTCAAAGACGTCGAAGTGACGCTACCCCCCCTTCGTGAACGTCGATCCGAC
ATCCCGCTTCTCGCCCGTTTCTTCCTGGCCGTTGCTGCAGATGAATTCGGACGTCACTGCCCCAGCATGACAACAGAGGC
TGAAGAACTGCTCCAGCAGCAGCAATGGCCCGGCAACCTGCGTCAGCTCAAGCAACTCATGCGCAGGGCCCTGTTCATGG
CGGGAAACCCCATCTCCGACGCTGGTCTCAAGCCCCTCATCGGCCAGGCCACCAGCCCCATAGTCCCTGACTTACTGCCA
TCGTCTCTCGACCTGGTGGAACTGGAAAAATGGGCCATCATCCAGGCATTGGCGGCCACCGACGGGAAAAAGCAACAGGC
CGCCCGGTTGCTGGGAGTCAGTTACAACACCTTCAAGGATAAAATCCGCCGTTACAACCTCACCGGCCTCTGA

Upstream 100 bases:

>100_bases
ATGATCATCGAGGATAGCATGGGGGGTAAAATGTCCTTTTCCAGTGAACCGGGTGAGACGGATTTCACTATCATCCTCCC
CGCGGCAGAAGAGGCGTGAT

Downstream 100 bases:

>100_bases
TCCCTATCAGAAATTGATACTTTCAGTGTCAACTTCTGATAGGGGGATTCACCCCCCCACCCACCTAACACCCAGTAAAT
TAAAATAAAACAAAAAAATG

Product: Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MGELTDVTILYVEDEPVLREQVVFALRMHFSRVLSACDGKEAMRMIRAECPDVVVSDIRMPAMDGLTLASLLKTELPDLP
VVLCTALHETDHLLKAIELGVAAYVSKPIDLEKLQHAIRLASLPCVNRREIARLRSEAAISNGLLGEGSIMRPIAEQLQR
VADTDLSVMLLGEPGTGKNFLAAKVHALSKRKTQPFLAVDCLARTPEQLEKELFGEAKGRGRPSSGATELLSEADGGTLL
LDGPERLPLQLQQRLFNLLEDGMFVPNGSIRPVACNLRVITVTGANLDKLAQAGTFSQELLERLKDVEVTLPPLRERRSD
IPLLARFFLAVAADEFGRHCPSMTTEAEELLQQQQWPGNLRQLKQLMRRALFMAGNPISDAGLKPLIGQATSPIVPDLLP
SSLDLVELEKWAIIQALAATDGKKQQAARLLGVSYNTFKDKIRRYNLTGL

Sequences:

>Translated_450_residues
MGELTDVTILYVEDEPVLREQVVFALRMHFSRVLSACDGKEAMRMIRAECPDVVVSDIRMPAMDGLTLASLLKTELPDLP
VVLCTALHETDHLLKAIELGVAAYVSKPIDLEKLQHAIRLASLPCVNRREIARLRSEAAISNGLLGEGSIMRPIAEQLQR
VADTDLSVMLLGEPGTGKNFLAAKVHALSKRKTQPFLAVDCLARTPEQLEKELFGEAKGRGRPSSGATELLSEADGGTLL
LDGPERLPLQLQQRLFNLLEDGMFVPNGSIRPVACNLRVITVTGANLDKLAQAGTFSQELLERLKDVEVTLPPLRERRSD
IPLLARFFLAVAADEFGRHCPSMTTEAEELLQQQQWPGNLRQLKQLMRRALFMAGNPISDAGLKPLIGQATSPIVPDLLP
SSLDLVELEKWAIIQALAATDGKKQQAARLLGVSYNTFKDKIRRYNLTGL
>Mature_449_residues
GELTDVTILYVEDEPVLREQVVFALRMHFSRVLSACDGKEAMRMIRAECPDVVVSDIRMPAMDGLTLASLLKTELPDLPV
VLCTALHETDHLLKAIELGVAAYVSKPIDLEKLQHAIRLASLPCVNRREIARLRSEAAISNGLLGEGSIMRPIAEQLQRV
ADTDLSVMLLGEPGTGKNFLAAKVHALSKRKTQPFLAVDCLARTPEQLEKELFGEAKGRGRPSSGATELLSEADGGTLLL
DGPERLPLQLQQRLFNLLEDGMFVPNGSIRPVACNLRVITVTGANLDKLAQAGTFSQELLERLKDVEVTLPPLRERRSDI
PLLARFFLAVAADEFGRHCPSMTTEAEELLQQQQWPGNLRQLKQLMRRALFMAGNPISDAGLKPLIGQATSPIVPDLLPS
SLDLVELEKWAIIQALAATDGKKQQAARLLGVSYNTFKDKIRRYNLTGL

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=440, Percent_Identity=35.4545454545455, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=32.8976034858388, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=32.662192393736, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=32.739420935412, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=37.5, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=328, Percent_Identity=32.9268292682927, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI87082117, Length=341, Percent_Identity=33.1378299120235, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787583, Length=302, Percent_Identity=33.112582781457, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=34.2857142857143, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789087, Length=396, Percent_Identity=29.5454545454545, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=30.4794520547945, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87082152, Length=242, Percent_Identity=34.7107438016529, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789828, Length=262, Percent_Identity=30.1526717557252, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49483; Mature: 49352

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELTDVTILYVEDEPVLREQVVFALRMHFSRVLSACDGKEAMRMIRAECPDVVVSDIRM
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCC
PAMDGLTLASLLKTELPDLPVVLCTALHETDHLLKAIELGVAAYVSKPIDLEKLQHAIRL
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ASLPCVNRREIARLRSEAAISNGLLGEGSIMRPIAEQLQRVADTDLSVMLLGEPGTGKNF
HCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
LAAKVHALSKRKTQPFLAVDCLARTPEQLEKELFGEAKGRGRPSSGATELLSEADGGTLL
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEE
LDGPERLPLQLQQRLFNLLEDGMFVPNGSIRPVACNLRVITVTGANLDKLAQAGTFSQEL
ECCCCCCCHHHHHHHHHHHHCCEECCCCCCCEEEEEEEEEEEECCCHHHHHHCCCHHHHH
LERLKDVEVTLPPLRERRSDIPLLARFFLAVAADEFGRHCPSMTTEAEELLQQQQWPGNL
HHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
RQLKQLMRRALFMAGNPISDAGLKPLIGQATSPIVPDLLPSSLDLVELEKWAIIQALAAT
HHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHC
DGKKQQAARLLGVSYNTFKDKIRRYNLTGL
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GELTDVTILYVEDEPVLREQVVFALRMHFSRVLSACDGKEAMRMIRAECPDVVVSDIRM
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCC
PAMDGLTLASLLKTELPDLPVVLCTALHETDHLLKAIELGVAAYVSKPIDLEKLQHAIRL
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ASLPCVNRREIARLRSEAAISNGLLGEGSIMRPIAEQLQRVADTDLSVMLLGEPGTGKNF
HCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
LAAKVHALSKRKTQPFLAVDCLARTPEQLEKELFGEAKGRGRPSSGATELLSEADGGTLL
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEE
LDGPERLPLQLQQRLFNLLEDGMFVPNGSIRPVACNLRVITVTGANLDKLAQAGTFSQEL
ECCCCCCCHHHHHHHHHHHHCCEECCCCCCCEEEEEEEEEEEECCCHHHHHHCCCHHHHH
LERLKDVEVTLPPLRERRSDIPLLARFFLAVAADEFGRHCPSMTTEAEELLQQQQWPGNL
HHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
RQLKQLMRRALFMAGNPISDAGLKPLIGQATSPIVPDLLPSSLDLVELEKWAIIQALAAT
HHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHC
DGKKQQAARLLGVSYNTFKDKIRRYNLTGL
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]