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The map label for this gene is trmD

Identifier: 38234107

GI number: 38234107

Start: 1551703

End: 1552581

Strand: Reverse

Name: trmD

Synonym: DIP1530

Alternate gene names: 38234107

Gene position: 1552581-1551703 (Counterclockwise)

Preceding gene: 38234108

Following gene: 38234106

Centisome position: 62.39

GC content: 50.74

Gene sequence:

>879_bases
ATGAGACTTGATGTCATCACAATTTTCCCTGAATATCTTGATCCGCTAAGACACGCACTTTTAGGGAAAGCGATCGAGAA
AGATTTGCTTAGTGTCGGTGTTCATGATCTGCGGTTGTGGGCTGAAGATGCGCATAAGTCTGTCGACGATTCTCCCTTCG
GCGGTGGGCCAGGAATGGTGATGAAGCCAACGGTGTGGGGACCAGCGCTTGATGATGTAGCTACCATGTCGGGTAAAGCG
CACATGGGTGCGCAATTAGATTCGGCACGCGTGCACGTCGATAAGCCTCGTCATGATGAGCTAGAAGGTATTCAATTTGC
AGGTTATGATGCCGCAGAGGTAGCAGAAGCCGATAAACCGTTGCTTTTGGTTCCCACGCCAGCTGGTGCACCCTTTACTC
AAGAAGATGCACGCGCGTGGTCTAACGAAGAGCACATTGTTTTTGCTTGTGGGCGATACGAAGGCATCGATCAACGCGTT
ATTGAAGATGCTAAGAAAACCTATCGAGTACGTGAAGTCTCCATCGGCGATTATGTTCTCATCGGTGGAGAAGTTGCCGT
TCTTGTCATCGCTGAAGCCGTAGTTCGCCTCATCCCAGGTGTCCTTGGAAATACTCAGTCGCATCAAGACGATAGCTTTT
CCGACGGCTTGTTGGAAGGCCCAAGCTATACCAAACCTCGTGAATGGCGTGGACTCGAAGTACCTGAAGTGCTCACTAGC
GGCAATCATGCCAAGATTGAACGGTGGAGGCGAGAACAATCCCTCAAACGCACGTGGGAAGTACGCCCAGAATTGCTGGA
TGGTATGGAGCTGGATCGACACGACCAAGCCTATGTAGAAGGCCTGCGGCGAGGAAACACCAGTGATAATCTCAACTGA

Upstream 100 bases:

>100_bases
AAGAGGTTCTTATTCCATTCGTCCACGATATCGTTCCCGAAGTTGATCTTGACGCTGGAACGTGTGTGATTACTCCACCG
GAAGGGCTGCTGGAACTCTA

Downstream 100 bases:

>100_bases
TCTTCAGCTGCTGATTTCTCCTATGCAATGGGATCAGGTGGCGTCTGAAATTGCACAACAACTTTCAGCTGTGGGATACA
ACGTTACGGATATTCGTGCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRLDVITIFPEYLDPLRHALLGKAIEKDLLSVGVHDLRLWAEDAHKSVDDSPFGGGPGMVMKPTVWGPALDDVATMSGKA
HMGAQLDSARVHVDKPRHDELEGIQFAGYDAAEVAEADKPLLLVPTPAGAPFTQEDARAWSNEEHIVFACGRYEGIDQRV
IEDAKKTYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNTQSHQDDSFSDGLLEGPSYTKPREWRGLEVPEVLTS
GNHAKIERWRREQSLKRTWEVRPELLDGMELDRHDQAYVEGLRRGNTSDNLN

Sequences:

>Translated_292_residues
MRLDVITIFPEYLDPLRHALLGKAIEKDLLSVGVHDLRLWAEDAHKSVDDSPFGGGPGMVMKPTVWGPALDDVATMSGKA
HMGAQLDSARVHVDKPRHDELEGIQFAGYDAAEVAEADKPLLLVPTPAGAPFTQEDARAWSNEEHIVFACGRYEGIDQRV
IEDAKKTYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNTQSHQDDSFSDGLLEGPSYTKPREWRGLEVPEVLTS
GNHAKIERWRREQSLKRTWEVRPELLDGMELDRHDQAYVEGLRRGNTSDNLN
>Mature_292_residues
MRLDVITIFPEYLDPLRHALLGKAIEKDLLSVGVHDLRLWAEDAHKSVDDSPFGGGPGMVMKPTVWGPALDDVATMSGKA
HMGAQLDSARVHVDKPRHDELEGIQFAGYDAAEVAEADKPLLLVPTPAGAPFTQEDARAWSNEEHIVFACGRYEGIDQRV
IEDAKKTYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNTQSHQDDSFSDGLLEGPSYTKPREWRGLEVPEVLTS
GNHAKIERWRREQSLKRTWEVRPELLDGMELDRHDQAYVEGLRRGNTSDNLN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=272, Percent_Identity=37.1323529411765, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CORDI (Q6NGI5)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939874.1
- ProteinModelPortal:   Q6NGI5
- SMR:   Q6NGI5
- GeneID:   2650720
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1530
- NMPDR:   fig|257309.1.peg.1465
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   CDIP257309:DIP1530-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 32418; Mature: 32418

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDVITIFPEYLDPLRHALLGKAIEKDLLSVGVHDLRLWAEDAHKSVDDSPFGGGPGMV
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCE
MKPTVWGPALDDVATMSGKAHMGAQLDSARVHVDKPRHDELEGIQFAGYDAAEVAEADKP
ECCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEECCCCHHHHHCCCCC
LLLVPTPAGAPFTQEDARAWSNEEHIVFACGRYEGIDQRVIEDAKKTYRVREVSIGDYVL
EEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCEEE
IGGEVAVLVIAEAVVRLIPGVLGNTQSHQDDSFSDGLLEGPSYTKPREWRGLEVPEVLTS
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
GNHAKIERWRREQSLKRTWEVRPELLDGMELDRHDQAYVEGLRRGNTSDNLN
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLDVITIFPEYLDPLRHALLGKAIEKDLLSVGVHDLRLWAEDAHKSVDDSPFGGGPGMV
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCE
MKPTVWGPALDDVATMSGKAHMGAQLDSARVHVDKPRHDELEGIQFAGYDAAEVAEADKP
ECCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEECCCCHHHHHCCCCC
LLLVPTPAGAPFTQEDARAWSNEEHIVFACGRYEGIDQRVIEDAKKTYRVREVSIGDYVL
EEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCEEE
IGGEVAVLVIAEAVVRLIPGVLGNTQSHQDDSFSDGLLEGPSYTKPREWRGLEVPEVLTS
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
GNHAKIERWRREQSLKRTWEVRPELLDGMELDRHDQAYVEGLRRGNTSDNLN
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910