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The map label for this gene is metK

Identifier: 38233911

GI number: 38233911

Start: 1337610

End: 1338842

Strand: Reverse

Name: metK

Synonym: DIP1325

Alternate gene names: 38233911

Gene position: 1338842-1337610 (Counterclockwise)

Preceding gene: 38233912

Following gene: 38233910

Centisome position: 53.8

GC content: 50.45

Gene sequence:

>1233_bases
GTGTCTGAAACTGCTGCCTCCGGCTTGCGTTTGTTTACTAGCGAATCGGTTACCGAGGGGCATCCAGACAAAATCTGCGA
TGCGATCTCGGACACCATATTGGATGCCTTGCTGAGCGTTGATCCTCACGCTCGCGTGGCTGTGGAGACTGTCACCACAA
CAGGTTTAGTTCACGTCGTGGGAGAAGTTCGTACCTCCGGCTACGTGGAAATTCCAAAATTGGTTCGTGACAAACTAATT
GAAATTGGGTTTAACTCTTCTGAGGTTGGCTTCGACGGTCGCACCTGTGGTGTGAGCGTATCCATTGGTGAACAGTCTCA
GGAAATTGGTGCTGGTGTTGATCAGTCTCATGAGGTTCGTTCGGGCGAGAATACAGATGCAGATGACCAGGCTGGAGCGG
GCGATCAGGGGTTAATGTTCGGCTACGCTACGAACGAGACAGCCACGTTGATGCCTTTGCCTATTGATTTGGCGCATCGA
CTTGCTCGTCGCCTGACTCAGGTGCGTAAGACCTCTGTTGTTTCCCATTTGCGTCCTGATGGAAAAACTCAGGTAACACT
TGCGTATGACGAATCAGGTCGCCCGGTACGTTTGGATACGGTAGTTGTTTCAACTCAGCATGATCCTGATGTAACCCAGG
AATGGCTGTTTGCTCAGATTAAAGAGCATGTTGTTGACTGGGTGCTTCGCGATTCAGGCTTGGAGTCCACACTCGATATC
AGCGGTTATTCATTGCTGGTTAATCCATCGGGTTCCTTTGTTGTTGGTGGCCCCATGGGCGATGCTGGGTTGACGGGACG
AAAGATCATCGTCGATACCTATGGCGGCATGGCGCGGCATGGTGGCGGTGCGTTCTCTGGAAAAGATCCTAGTAAAGTAG
ACCGTTCAGGTGCGTACGCTATGCGTTGGGTTGCTAAGAATATTGTTGCTGCGGGATTGGCTGATCGAGCAGAGGTACAG
GTTGCTTATGCGATTGGTCGTGCAAAACCTGTCGGTTTGTATGTCGAAACTTTTGGAACGGCCAAATTTGGTTTGACGGA
TGAACAGATTCAAGATGCAGTATTGCACGTGTTCGATCTTCGTCCTGCAGCGATTATTCGAGAGTTGGATCTTCTTCGTC
CGATTTATGCTGGTACGGCCGCTTATGGGCATTTCGGACGCGATGATCTGAAGCTGCCTTGGGAAAATACGGATCGAGCA
GAACAGTTACGTGACGTGTTGTCTTTAGGCTAA

Upstream 100 bases:

>100_bases
TATTGGGTGAAATCAGCTTAAACGGTGAGGTCTTTATTGCGGAAAAGCTTTGTCTGACGGAAGCCGTTTAAATCAATCTT
TTTCACTGAAGGGCAATCGA

Downstream 100 bases:

>100_bases
ATATGTAGAATCGTTGCCATGTCTAAAACCCGCGTAGCTGCTTCAAGTCTTCCAGTTGCGCGGGTGTTGCCATTACTAGG
CGTTGCTCATCTGGATCGCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSETAASGLRLFTSESVTEGHPDKICDAISDTILDALLSVDPHARVAVETVTTTGLVHVVGEVRTSGYVEIPKLVRDKLI
EIGFNSSEVGFDGRTCGVSVSIGEQSQEIGAGVDQSHEVRSGENTDADDQAGAGDQGLMFGYATNETATLMPLPIDLAHR
LARRLTQVRKTSVVSHLRPDGKTQVTLAYDESGRPVRLDTVVVSTQHDPDVTQEWLFAQIKEHVVDWVLRDSGLESTLDI
SGYSLLVNPSGSFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNIVAAGLADRAEVQ
VAYAIGRAKPVGLYVETFGTAKFGLTDEQIQDAVLHVFDLRPAAIIRELDLLRPIYAGTAAYGHFGRDDLKLPWENTDRA
EQLRDVLSLG

Sequences:

>Translated_410_residues
MSETAASGLRLFTSESVTEGHPDKICDAISDTILDALLSVDPHARVAVETVTTTGLVHVVGEVRTSGYVEIPKLVRDKLI
EIGFNSSEVGFDGRTCGVSVSIGEQSQEIGAGVDQSHEVRSGENTDADDQAGAGDQGLMFGYATNETATLMPLPIDLAHR
LARRLTQVRKTSVVSHLRPDGKTQVTLAYDESGRPVRLDTVVVSTQHDPDVTQEWLFAQIKEHVVDWVLRDSGLESTLDI
SGYSLLVNPSGSFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNIVAAGLADRAEVQ
VAYAIGRAKPVGLYVETFGTAKFGLTDEQIQDAVLHVFDLRPAAIIRELDLLRPIYAGTAAYGHFGRDDLKLPWENTDRA
EQLRDVLSLG
>Mature_409_residues
SETAASGLRLFTSESVTEGHPDKICDAISDTILDALLSVDPHARVAVETVTTTGLVHVVGEVRTSGYVEIPKLVRDKLIE
IGFNSSEVGFDGRTCGVSVSIGEQSQEIGAGVDQSHEVRSGENTDADDQAGAGDQGLMFGYATNETATLMPLPIDLAHRL
ARRLTQVRKTSVVSHLRPDGKTQVTLAYDESGRPVRLDTVVVSTQHDPDVTQEWLFAQIKEHVVDWVLRDSGLESTLDIS
GYSLLVNPSGSFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNIVAAGLADRAEVQV
AYAIGRAKPVGLYVETFGTAKFGLTDEQIQDAVLHVFDLRPAAIIRELDLLRPIYAGTAAYGHFGRDDLKLPWENTDRAE
QLRDVLSLG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=393, Percent_Identity=51.1450381679389, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI5174529, Length=392, Percent_Identity=51.0204081632653, Blast_Score=349, Evalue=3e-96,
Organism=Escherichia coli, GI1789311, Length=399, Percent_Identity=56.390977443609, Blast_Score=433, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17538498, Length=401, Percent_Identity=51.8703241895262, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17551082, Length=390, Percent_Identity=53.5897435897436, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=52.1850899742931, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145633, Length=399, Percent_Identity=51.6290726817043, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=48.0719794344473, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI32565911, Length=356, Percent_Identity=48.876404494382, Blast_Score=303, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI25145635, Length=354, Percent_Identity=48.5875706214689, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI32565903, Length=316, Percent_Identity=50.6329113924051, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=53.0120481927711, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323209, Length=394, Percent_Identity=53.0456852791878, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6320710, Length=394, Percent_Identity=49.746192893401, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI45552159, Length=394, Percent_Identity=48.9847715736041, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580529, Length=394, Percent_Identity=48.9847715736041, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580537, Length=394, Percent_Identity=48.9847715736041, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580533, Length=394, Percent_Identity=48.9847715736041, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580535, Length=394, Percent_Identity=48.9847715736041, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580531, Length=394, Percent_Identity=48.9847715736041, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580527, Length=388, Percent_Identity=48.4536082474227, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24580525, Length=388, Percent_Identity=48.4536082474227, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24580541, Length=368, Percent_Identity=47.554347826087, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24580539, Length=136, Percent_Identity=44.8529411764706, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CORDI (P61945)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939678.1
- ProteinModelPortal:   P61945
- GeneID:   2649879
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1325
- NMPDR:   fig|257309.1.peg.1269
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   P61945
- ProtClustDB:   PRK05250
- BioCyc:   CDIP257309:DIP1325-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44015; Mature: 43884

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETAASGLRLFTSESVTEGHPDKICDAISDTILDALLSVDPHARVAVETVTTTGLVHVV
CCCCHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
GEVRTSGYVEIPKLVRDKLIEIGFNSSEVGFDGRTCGVSVSIGEQSQEIGAGVDQSHEVR
HHHCCCCCEECHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCC
SGENTDADDQAGAGDQGLMFGYATNETATLMPLPIDLAHRLARRLTQVRKTSVVSHLRPD
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
GKTQVTLAYDESGRPVRLDTVVVSTQHDPDVTQEWLFAQIKEHVVDWVLRDSGLESTLDI
CCEEEEEEECCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
SGYSLLVNPSGSFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYA
CCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHCCCHHH
MRWVAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETFGTAKFGLTDEQIQDAVLHVFDL
HHHHHHHHHHHCCCCCHHEEEEEEECCCCCCEEEEEECCCEECCCCHHHHHHHHHHHHCC
RPAAIIRELDLLRPIYAGTAAYGHFGRDDLKLPWENTDRAEQLRDVLSLG
CHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SETAASGLRLFTSESVTEGHPDKICDAISDTILDALLSVDPHARVAVETVTTTGLVHVV
CCCHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
GEVRTSGYVEIPKLVRDKLIEIGFNSSEVGFDGRTCGVSVSIGEQSQEIGAGVDQSHEVR
HHHCCCCCEECHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCC
SGENTDADDQAGAGDQGLMFGYATNETATLMPLPIDLAHRLARRLTQVRKTSVVSHLRPD
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
GKTQVTLAYDESGRPVRLDTVVVSTQHDPDVTQEWLFAQIKEHVVDWVLRDSGLESTLDI
CCEEEEEEECCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
SGYSLLVNPSGSFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYA
CCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHCCCHHH
MRWVAKNIVAAGLADRAEVQVAYAIGRAKPVGLYVETFGTAKFGLTDEQIQDAVLHVFDL
HHHHHHHHHHHCCCCCHHEEEEEEECCCCCCEEEEEECCCEECCCCHHHHHHHHHHHHCC
RPAAIIRELDLLRPIYAGTAAYGHFGRDDLKLPWENTDRAEQLRDVLSLG
CHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910