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The map label for this gene is priA [H]

Identifier: 38233910

GI number: 38233910

Start: 1335564

End: 1337591

Strand: Reverse

Name: priA [H]

Synonym: DIP1324

Alternate gene names: 38233910

Gene position: 1337591-1335564 (Counterclockwise)

Preceding gene: 38233911

Following gene: 38233909

Centisome position: 53.75

GC content: 48.57

Gene sequence:

>2028_bases
ATGTCTAAAACCCGCGTAGCTGCTTCAAGTCTTCCAGTTGCGCGGGTGTTGCCATTACTAGGCGTTGCTCATCTGGATCG
CGTTTTCGAATATCTAGTGGATTCGACGATGTCGGATGATGCTCAGCCAGGTGTCAAAGTTCGAGTACGATTCGGTGGCA
GGCTGGTTGACGGAATTATTTTGGAACGAACATCAGGTCAGGAACATGAGGGGAGACTTGCTTGGCTAGATAAAGTACAG
TCGTCCGTTCCCGTCTATACTCCAGCGTTGCGACGACTCATCGAATCTTTGTCCACTCGATATGCGGGGATCACTTCAGA
TGTGATCCGTTCGGCAATCCCTTCGAGACATGCAAAAGTAGAAGAAGGGGATTTTTCCACTAGTTGGGACGATCTAGGTG
ATGTCGATGAACCTGATTTGTCTATGTGGGCAAAATACGTTCATGGGAAATCATTCGTGGACGCTGTAATTGCGTGTCAA
ACCGCTCGTGCTGCTTGGCAAATTGCACCGGGGGAAAACTGGGCAGCTGCGATTGCAGCGTTGGGTACTAAAGTTGCGAT
TGCGGGTGGTGGAGTACTCATCGTTGTTCCTGATCAACGGGATGTAGAAACATTAGAAGCTGCATTTCGTGGTTTGGTAT
CTGCTCGACAAATAACGGTGATGACATCCGCTCAAGGACCGCAGGCTCGCTATCGGCGGTTTTTAAGTATTTTGCATGGA
CAAGGCCGAATCGTTGTTGGCACTCGGAGTGCCGCATTTGCTCCTGTTGTCAACCTACAATTAGCAGTTATTAAAGACGA
TGGAGATGAAAATCTAGTTGATCCACGTAGCCCTTACATTCATGCGCGTGAAGTCCTGAGTACACGGTCTACACAAGAGC
ATTGTTCGCTTATTCTCGCAGGGCATTCGCGTAGTACCGAGGCGCAACTTTTGGTCCAATCAGGTTGGGCTCATAATCTA
GTAGTCGATCGCGAACGAGTGCGAAAAATGATGCCGCGCATTCATGCTGTTGCAGATACGAACACAGCGTTGGAACGTGA
TCCACTTGCGAGGGCTGCGCGGATTCCTTCAGCAGCTTTTTCTTCATTAAGAGATGCATTGAAGCGAAATCGACCGGTGC
TTATTCACGTCCCTAGGAAAGGGTATATTCCTGTACTAGCTTGTCAACAGTGCCGTTCTGCCGCGCGTTGTAGATATTGC
AATGGTCCCTTGGAATTGCCATTTCCGGAATCTGGTGCACCACAAACACCTCAATGTCGATGGTGTGGTCGGCCTGATAG
TCATTTTCGTTGTACCCATTGTGGATCCTCGAATCTTAGGGCAATTGTATTGGGTGCCGAACGGACTGCTGAAGAAATCG
GTCGAGCATTTCCTCAAACTCGAGTAGTTGTTTCTGGTGGAAACCGAGTGCACGATACGATCCCAGATGCGCCAATAGTG
GTGGTAGCTACCCCTGGAGCTGAACCAGAAATAGCGGACGATGGACGTTATGGAGCTGCAGTGTTGCTTGATACTTGGGC
TTTACTGAGCCGTCAAGATTTGAGAGCTTCCGAAGACACACTGGCAAAATGGTTTTCTGTATGCACTTTAGTTGAGCCCT
GTGTGGTGGGCGGAGAAGTCGTTGTTGTAGCAGATGCAGGACTTTTCGAAGTTCAAGCATTAATTCGATGGGATCCCATT
TGGGCTGCATCGCGTGAGCTTGAACTAAGACAAGAAGTACATTTTCCGCCTGCTGCTCACATGGCGGCTATCGATGGTGC
TCGAGATGCTATAGATGAATTTTTGGATATGATTCAACTTCCTGACCATGCAGAAATTTTGGGGCCGGTGAGCCTTCCTG
CAGGAAACAAACTTCCGGGCGAATACGATGAAAATACCTATGGTCCGCCACAACGCGTGTTGATTCGTATTCCATTGAAA
GACCGTAATGAACTAGGGGAAGTTTTACGATCTGGGATTGTGCAACGATCTGCTAAACGTAAAGAATTACCTCTCCGCGT
GAAAATCGATCCTATCCATGTAGGGTAG

Upstream 100 bases:

>100_bases
TTTCGGACGCGATGATCTGAAGCTGCCTTGGGAAAATACGGATCGAGCAGAACAGTTACGTGACGTGTTGTCTTTAGGCT
AAATATGTAGAATCGTTGCC

Downstream 100 bases:

>100_bases
ATGTTTTGGCGTGAGCCTATATATGTGGACTCTATTTGATTTGTACTTGGCAGAATAAGAAGAGACTAAAGGAAACAAAG
TGGCAATCCGCGATATTCGA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MSKTRVAASSLPVARVLPLLGVAHLDRVFEYLVDSTMSDDAQPGVKVRVRFGGRLVDGIILERTSGQEHEGRLAWLDKVQ
SSVPVYTPALRRLIESLSTRYAGITSDVIRSAIPSRHAKVEEGDFSTSWDDLGDVDEPDLSMWAKYVHGKSFVDAVIACQ
TARAAWQIAPGENWAAAIAALGTKVAIAGGGVLIVVPDQRDVETLEAAFRGLVSARQITVMTSAQGPQARYRRFLSILHG
QGRIVVGTRSAAFAPVVNLQLAVIKDDGDENLVDPRSPYIHAREVLSTRSTQEHCSLILAGHSRSTEAQLLVQSGWAHNL
VVDRERVRKMMPRIHAVADTNTALERDPLARAARIPSAAFSSLRDALKRNRPVLIHVPRKGYIPVLACQQCRSAARCRYC
NGPLELPFPESGAPQTPQCRWCGRPDSHFRCTHCGSSNLRAIVLGAERTAEEIGRAFPQTRVVVSGGNRVHDTIPDAPIV
VVATPGAEPEIADDGRYGAAVLLDTWALLSRQDLRASEDTLAKWFSVCTLVEPCVVGGEVVVVADAGLFEVQALIRWDPI
WAASRELELRQEVHFPPAAHMAAIDGARDAIDEFLDMIQLPDHAEILGPVSLPAGNKLPGEYDENTYGPPQRVLIRIPLK
DRNELGEVLRSGIVQRSAKRKELPLRVKIDPIHVG

Sequences:

>Translated_675_residues
MSKTRVAASSLPVARVLPLLGVAHLDRVFEYLVDSTMSDDAQPGVKVRVRFGGRLVDGIILERTSGQEHEGRLAWLDKVQ
SSVPVYTPALRRLIESLSTRYAGITSDVIRSAIPSRHAKVEEGDFSTSWDDLGDVDEPDLSMWAKYVHGKSFVDAVIACQ
TARAAWQIAPGENWAAAIAALGTKVAIAGGGVLIVVPDQRDVETLEAAFRGLVSARQITVMTSAQGPQARYRRFLSILHG
QGRIVVGTRSAAFAPVVNLQLAVIKDDGDENLVDPRSPYIHAREVLSTRSTQEHCSLILAGHSRSTEAQLLVQSGWAHNL
VVDRERVRKMMPRIHAVADTNTALERDPLARAARIPSAAFSSLRDALKRNRPVLIHVPRKGYIPVLACQQCRSAARCRYC
NGPLELPFPESGAPQTPQCRWCGRPDSHFRCTHCGSSNLRAIVLGAERTAEEIGRAFPQTRVVVSGGNRVHDTIPDAPIV
VVATPGAEPEIADDGRYGAAVLLDTWALLSRQDLRASEDTLAKWFSVCTLVEPCVVGGEVVVVADAGLFEVQALIRWDPI
WAASRELELRQEVHFPPAAHMAAIDGARDAIDEFLDMIQLPDHAEILGPVSLPAGNKLPGEYDENTYGPPQRVLIRIPLK
DRNELGEVLRSGIVQRSAKRKELPLRVKIDPIHVG
>Mature_674_residues
SKTRVAASSLPVARVLPLLGVAHLDRVFEYLVDSTMSDDAQPGVKVRVRFGGRLVDGIILERTSGQEHEGRLAWLDKVQS
SVPVYTPALRRLIESLSTRYAGITSDVIRSAIPSRHAKVEEGDFSTSWDDLGDVDEPDLSMWAKYVHGKSFVDAVIACQT
ARAAWQIAPGENWAAAIAALGTKVAIAGGGVLIVVPDQRDVETLEAAFRGLVSARQITVMTSAQGPQARYRRFLSILHGQ
GRIVVGTRSAAFAPVVNLQLAVIKDDGDENLVDPRSPYIHAREVLSTRSTQEHCSLILAGHSRSTEAQLLVQSGWAHNLV
VDRERVRKMMPRIHAVADTNTALERDPLARAARIPSAAFSSLRDALKRNRPVLIHVPRKGYIPVLACQQCRSAARCRYCN
GPLELPFPESGAPQTPQCRWCGRPDSHFRCTHCGSSNLRAIVLGAERTAEEIGRAFPQTRVVVSGGNRVHDTIPDAPIVV
VATPGAEPEIADDGRYGAAVLLDTWALLSRQDLRASEDTLAKWFSVCTLVEPCVVGGEVVVVADAGLFEVQALIRWDPIW
AASRELELRQEVHFPPAAHMAAIDGARDAIDEFLDMIQLPDHAEILGPVSLPAGNKLPGEYDENTYGPPQRVLIRIPLKD
RNELGEVLRSGIVQRSAKRKELPLRVKIDPIHVG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=361, Percent_Identity=26.5927977839335, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 73739; Mature: 73608

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTRVAASSLPVARVLPLLGVAHLDRVFEYLVDSTMSDDAQPGVKVRVRFGGRLVDGII
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEEEEEE
LERTSGQEHEGRLAWLDKVQSSVPVYTPALRRLIESLSTRYAGITSDVIRSAIPSRHAKV
EECCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
EEGDFSTSWDDLGDVDEPDLSMWAKYVHGKSFVDAVIACQTARAAWQIAPGENWAAAIAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHH
LGTKVAIAGGGVLIVVPDQRDVETLEAAFRGLVSARQITVMTSAQGPQARYRRFLSILHG
HCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
QGRIVVGTRSAAFAPVVNLQLAVIKDDGDENLVDPRSPYIHAREVLSTRSTQEHCSLILA
CCEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHCEEEEE
GHSRSTEAQLLVQSGWAHNLVVDRERVRKMMPRIHAVADTNTALERDPLARAARIPSAAF
CCCCCCCHHHHEECCCCCCEEECHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHCCHHHH
SSLRDALKRNRPVLIHVPRKGYIPVLACQQCRSAARCRYCNGPLELPFPESGAPQTPQCR
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCC
WCGRPDSHFRCTHCGSSNLRAIVLGAERTAEEIGRAFPQTRVVVSGGNRVHDTIPDAPIV
CCCCCCCCEEEEECCCCCCEEEEEECHHHHHHHHHHCCCCEEEEECCCEEECCCCCCCEE
VVATPGAEPEIADDGRYGAAVLLDTWALLSRQDLRASEDTLAKWFSVCTLVEPCVVGGEV
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCE
VVVADAGLFEVQALIRWDPIWAASRELELRQEVHFPPAAHMAAIDGARDAIDEFLDMIQL
EEEECCCHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHCC
PDHAEILGPVSLPAGNKLPGEYDENTYGPPQRVLIRIPLKDRNELGEVLRSGIVQRSAKR
CCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KELPLRVKIDPIHVG
CCCCEEEEEEEEECC
>Mature Secondary Structure 
SKTRVAASSLPVARVLPLLGVAHLDRVFEYLVDSTMSDDAQPGVKVRVRFGGRLVDGII
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEEEEEE
LERTSGQEHEGRLAWLDKVQSSVPVYTPALRRLIESLSTRYAGITSDVIRSAIPSRHAKV
EECCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
EEGDFSTSWDDLGDVDEPDLSMWAKYVHGKSFVDAVIACQTARAAWQIAPGENWAAAIAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHH
LGTKVAIAGGGVLIVVPDQRDVETLEAAFRGLVSARQITVMTSAQGPQARYRRFLSILHG
HCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
QGRIVVGTRSAAFAPVVNLQLAVIKDDGDENLVDPRSPYIHAREVLSTRSTQEHCSLILA
CCEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHCEEEEE
GHSRSTEAQLLVQSGWAHNLVVDRERVRKMMPRIHAVADTNTALERDPLARAARIPSAAF
CCCCCCCHHHHEECCCCCCEEECHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHCCHHHH
SSLRDALKRNRPVLIHVPRKGYIPVLACQQCRSAARCRYCNGPLELPFPESGAPQTPQCR
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCC
WCGRPDSHFRCTHCGSSNLRAIVLGAERTAEEIGRAFPQTRVVVSGGNRVHDTIPDAPIV
CCCCCCCCEEEEECCCCCCEEEEEECHHHHHHHHHHCCCCEEEEECCCEEECCCCCCCEE
VVATPGAEPEIADDGRYGAAVLLDTWALLSRQDLRASEDTLAKWFSVCTLVEPCVVGGEV
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCE
VVVADAGLFEVQALIRWDPIWAASRELELRQEVHFPPAAHMAAIDGARDAIDEFLDMIQL
EEEECCCHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHCC
PDHAEILGPVSLPAGNKLPGEYDENTYGPPQRVLIRIPLKDRNELGEVLRSGIVQRSAKR
CCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KELPLRVKIDPIHVG
CCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]