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The map label for this gene is def [H]

Identifier: 38233909

GI number: 38233909

Start: 1334975

End: 1335484

Strand: Reverse

Name: def [H]

Synonym: DIP1323

Alternate gene names: 38233909

Gene position: 1335484-1334975 (Counterclockwise)

Preceding gene: 38233910

Following gene: 38233908

Centisome position: 53.66

GC content: 46.47

Gene sequence:

>510_bases
GTGGCAATCCGCGATATTCGATTGTTTGGTGATCCCGTTTTGACTACGAAGTCGTCGGACGTGGAAGTCTTCGACTCCAG
TATTCGAAATTTGGTTAATGACATGTTGGAAACCATGGATGCGGCTGGCGGAGTTGGCTTGGCAGCTAACCAAGTCGGAG
TAACTAAACGAGTCTTTGTGTATGACTGTTCGCATATTGAGGATGGGCTTCGCGGGCATATCATCAACCCGGTTTGGGAG
CCCATTGGGGAAGATATTCAAATTGGTCCAGAAGGTTGCTTGTCCATTCCAGATGTTCAGGAAGAAACTGAGCGTTGGAT
GACAGTAAGCGTTAGTGGCCGTGATGTTGATGGAAACCCGATTTCACTGGTTGCTTCTGGCTTGATGGCACGGTGTATTC
AACATGAAACAGATCATCTAGATGGTGTTTTATTCTTGAGAAAGCTTGATAAAGTCCACCGGAAAGACGCAATGGCTCAG
ATCAGGAAGTCTGAGTGGTTTAATCAATAG

Upstream 100 bases:

>100_bases
GATCCTATCCATGTAGGGTAGATGTTTTGGCGTGAGCCTATATATGTGGACTCTATTTGATTTGTACTTGGCAGAATAAG
AAGAGACTAAAGGAAACAAA

Downstream 100 bases:

>100_bases
CTATAGAGTCCGACTCGAAGACTTAATGAGGAAAGTATGCGTCTAATTTTTGCTGGAACACCTGAGCCTGCAGTTGTAGT
TTTATCCCGATTGCTCGAAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MAIRDIRLFGDPVLTTKSSDVEVFDSSIRNLVNDMLETMDAAGGVGLAANQVGVTKRVFVYDCSHIEDGLRGHIINPVWE
PIGEDIQIGPEGCLSIPDVQEETERWMTVSVSGRDVDGNPISLVASGLMARCIQHETDHLDGVLFLRKLDKVHRKDAMAQ
IRKSEWFNQ

Sequences:

>Translated_169_residues
MAIRDIRLFGDPVLTTKSSDVEVFDSSIRNLVNDMLETMDAAGGVGLAANQVGVTKRVFVYDCSHIEDGLRGHIINPVWE
PIGEDIQIGPEGCLSIPDVQEETERWMTVSVSGRDVDGNPISLVASGLMARCIQHETDHLDGVLFLRKLDKVHRKDAMAQ
IRKSEWFNQ
>Mature_168_residues
AIRDIRLFGDPVLTTKSSDVEVFDSSIRNLVNDMLETMDAAGGVGLAANQVGVTKRVFVYDCSHIEDGLRGHIINPVWEP
IGEDIQIGPEGCLSIPDVQEETERWMTVSVSGRDVDGNPISLVASGLMARCIQHETDHLDGVLFLRKLDKVHRKDAMAQI
RKSEWFNQ

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=31.4465408805031, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=38.7878787878788, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645726, Length=160, Percent_Identity=31.25, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18775; Mature: 18643

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRDIRLFGDPVLTTKSSDVEVFDSSIRNLVNDMLETMDAAGGVGLAANQVGVTKRVFV
CCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
YDCSHIEDGLRGHIINPVWEPIGEDIQIGPEGCLSIPDVQEETERWMTVSVSGRDVDGNP
EECHHHHCCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCHHHHHCEEEEEECCCCCCCCH
ISLVASGLMARCIQHETDHLDGVLFLRKLDKVHRKDAMAQIRKSEWFNQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIRDIRLFGDPVLTTKSSDVEVFDSSIRNLVNDMLETMDAAGGVGLAANQVGVTKRVFV
CCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
YDCSHIEDGLRGHIINPVWEPIGEDIQIGPEGCLSIPDVQEETERWMTVSVSGRDVDGNP
EECHHHHCCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCHHHHHCEEEEEECCCCCCCCH
ISLVASGLMARCIQHETDHLDGVLFLRKLDKVHRKDAMAQIRKSEWFNQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]