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The map label for this gene is glgE [H]

Identifier: 38233660

GI number: 38233660

Start: 1046014

End: 1048050

Strand: Reverse

Name: glgE [H]

Synonym: DIP1066

Alternate gene names: 38233660

Gene position: 1048050-1046014 (Counterclockwise)

Preceding gene: 38233667

Following gene: 38233659

Centisome position: 42.11

GC content: 54.1

Gene sequence:

>2037_bases
ATGAGTTCGAAGTCCACACGACTTGCTATTGAAGATGTTCGCCCCCAGGTTTCGTGCGGGCGACGGCCGTCGAAAGCCGT
GGTCGGGGAAATGATTCCCGTCAGCGCTTTGGTGTGGCGCGAAGGCCACGATGCCATTGCCGCCACTCTCGTGGTAACTG
ATCCGACAGGTCAAAGTTCCTCCATTGCCATGGCGACCAAGAAGTTCGAGCCCGACCGTATGTGGGCAAGTTTCGTTCCT
GATCGCCCCGGTACGTGGACTTTTAGAGTCGATGCTTGGTCTGACGCCGTAGCCACATGGCGCCATGCCGTGACCACAAA
AATTGAGGCAGGTCAAAGTCCCGAGGAACTTTACAACGACATTGAGCATGGGGTTGCCCTATTTGAATCAGCCATCGCTA
GTGCGCCTAAGGCCGAAAGAAAGACTCTAGCGCCAGCGTTTACTCGGGTCATCAACGCACTGCGTAGCGACGCTCCCCTG
CGCACCCGAGTCGCTCCTGCACTCAGCGCAGAAATCACGTATCTGCTGGCTGAGCATCCTGTGCGCGAACTGCTCACCCG
CGGGCACACCTACTCAATTTCCGTTGAACGTACCAAGGCCCTGTATAGCTCATGGTACGAACTATTCCCGCGCTCAACTG
GCGGCTGGGACGATAACGGTCAGCCTGTTCACGGCACATTCGTTACCACAGCTCAGGCTTTGGATCGAGTGGCTGCCATG
GGTTTTGATACTGTCTATTTCCCACCAATCCACCCCATCGGTGAGATTAACCGCAAAGGCAAAAACAACACACTCATTGC
CCTACCTGGCGACGTAGGGTCACCATGGGCAATCGGCTCTGCTGAAGGTGGCCACGATTCAGTGCACCCCCGTCTAGGAA
CTATCGACGACTTCCGCGCGCTCATCGCCCGTGCACGCGAGCTGAACCTTGAAGTCGCACTTGATTTAGCGTTGCAAGCT
GCACCTGACCATCCATGGGCGAAATCTCACCCAGAGTTTTTCACCGTACTTGCTGACGGATCTATTGCTTTCGCAGAAAA
TCCACCCAAGAAATATCAAGATATCTACCCATTGAATTTTGATAACGCACACAAGAAGATCTATGCCGAAATTCTGCGCG
TCGTCCTTTTCTGGGTTCAGCAAGGTGTGACCACCTTCCGCGTAGATAACCCTCACACCAAACCAGCAAACTTCTGGGAA
TGGCTTATCGCCAAGGTTCATGCCAAGCACCCCGAAGTCATCTTCCTGGCGGAAGCTTTTACCCGCCCAGCGCGACTCTA
TGGCCTGGGCAAAGTGGGCTTTAGCCAGTCCTACACCTACTTCACGTGGAAGACTTCGAAGACTCAGCTGCAGCAATTCG
CCGAAGAAATCGCTGAGATGGCTGATGTTAGCCGCCCCAACCTGTTTGTTAACACCCCCGATATTTTGCACGAGTCACTG
CAGTATGGCGGTCGCGCCATGTTTGCTATTCGGGCAACGCTTGCAGCAACCATGTCGCCGGTATGGGGTGTGTACTCCGG
ATTCGAGCTCTTCGAGCATGAAGCAGTAGCCCACGGTAGTGAAGAGTATGCAAACTCCGAAAAATACGAACTACGGCCTC
GCAACTTTGACACTGCCCGCGCAGCGGGTAATTCTCTCGAACCGTATCTCACTTTGCTCAATTCGATTCGTGCACAGCAC
CCTGCTCTCCAGCAGCTACGTGTGATCGATTTCCACCCAACCGATAACGACTCCATTCTTGCCTACAGCAAGGTTGATCC
GGTCACCGGCGACACCATCGTGGTAGTAGTCAACCTAGATCCTGACCATGCTCAACAGGCAACACTTGAACTCGATATGG
AAGCCCTTGGGCGCGACACCACAGAATCCTTCGATGTCACCGATCTGGTGTCCGGACAGCAATTCCACTGGGGACAACGT
AATTTCATCCGCCTCGATCCGTGTGCCAACGTAGCTCATATCGTTGCTATTCCACCTGCACCGAATAGCCGCAAAGCGCA
ACTATCGTGGCGCGGAGAATCCGATTATCGGAACTAA

Upstream 100 bases:

>100_bases
GTGCATGTGGAATAGACACAGCTTGACTACTCTAAGAGACAAGGCTCGGGAAGGATTTTCCCGACAGTGAACAGTCCCCT
ACGCATAGAGGAATCGGCAT

Downstream 100 bases:

>100_bases
TAACAGTCAACATCCGCCTCATATCGACTTAACGTAAAGAAAGTACTTACTCTCATGGTTTCTCCTACTCAGATCACACC
AGTCCATCCTGATGTCCTCC

Product: putative alpha-amylase (glucanase)

Products: D-glucose; cholesterol

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSSKSTRLAIEDVRPQVSCGRRPSKAVVGEMIPVSALVWREGHDAIAATLVVTDPTGQSSSIAMATKKFEPDRMWASFVP
DRPGTWTFRVDAWSDAVATWRHAVTTKIEAGQSPEELYNDIEHGVALFESAIASAPKAERKTLAPAFTRVINALRSDAPL
RTRVAPALSAEITYLLAEHPVRELLTRGHTYSISVERTKALYSSWYELFPRSTGGWDDNGQPVHGTFVTTAQALDRVAAM
GFDTVYFPPIHPIGEINRKGKNNTLIALPGDVGSPWAIGSAEGGHDSVHPRLGTIDDFRALIARARELNLEVALDLALQA
APDHPWAKSHPEFFTVLADGSIAFAENPPKKYQDIYPLNFDNAHKKIYAEILRVVLFWVQQGVTTFRVDNPHTKPANFWE
WLIAKVHAKHPEVIFLAEAFTRPARLYGLGKVGFSQSYTYFTWKTSKTQLQQFAEEIAEMADVSRPNLFVNTPDILHESL
QYGGRAMFAIRATLAATMSPVWGVYSGFELFEHEAVAHGSEEYANSEKYELRPRNFDTARAAGNSLEPYLTLLNSIRAQH
PALQQLRVIDFHPTDNDSILAYSKVDPVTGDTIVVVVNLDPDHAQQATLELDMEALGRDTTESFDVTDLVSGQQFHWGQR
NFIRLDPCANVAHIVAIPPAPNSRKAQLSWRGESDYRN

Sequences:

>Translated_678_residues
MSSKSTRLAIEDVRPQVSCGRRPSKAVVGEMIPVSALVWREGHDAIAATLVVTDPTGQSSSIAMATKKFEPDRMWASFVP
DRPGTWTFRVDAWSDAVATWRHAVTTKIEAGQSPEELYNDIEHGVALFESAIASAPKAERKTLAPAFTRVINALRSDAPL
RTRVAPALSAEITYLLAEHPVRELLTRGHTYSISVERTKALYSSWYELFPRSTGGWDDNGQPVHGTFVTTAQALDRVAAM
GFDTVYFPPIHPIGEINRKGKNNTLIALPGDVGSPWAIGSAEGGHDSVHPRLGTIDDFRALIARARELNLEVALDLALQA
APDHPWAKSHPEFFTVLADGSIAFAENPPKKYQDIYPLNFDNAHKKIYAEILRVVLFWVQQGVTTFRVDNPHTKPANFWE
WLIAKVHAKHPEVIFLAEAFTRPARLYGLGKVGFSQSYTYFTWKTSKTQLQQFAEEIAEMADVSRPNLFVNTPDILHESL
QYGGRAMFAIRATLAATMSPVWGVYSGFELFEHEAVAHGSEEYANSEKYELRPRNFDTARAAGNSLEPYLTLLNSIRAQH
PALQQLRVIDFHPTDNDSILAYSKVDPVTGDTIVVVVNLDPDHAQQATLELDMEALGRDTTESFDVTDLVSGQQFHWGQR
NFIRLDPCANVAHIVAIPPAPNSRKAQLSWRGESDYRN
>Mature_677_residues
SSKSTRLAIEDVRPQVSCGRRPSKAVVGEMIPVSALVWREGHDAIAATLVVTDPTGQSSSIAMATKKFEPDRMWASFVPD
RPGTWTFRVDAWSDAVATWRHAVTTKIEAGQSPEELYNDIEHGVALFESAIASAPKAERKTLAPAFTRVINALRSDAPLR
TRVAPALSAEITYLLAEHPVRELLTRGHTYSISVERTKALYSSWYELFPRSTGGWDDNGQPVHGTFVTTAQALDRVAAMG
FDTVYFPPIHPIGEINRKGKNNTLIALPGDVGSPWAIGSAEGGHDSVHPRLGTIDDFRALIARARELNLEVALDLALQAA
PDHPWAKSHPEFFTVLADGSIAFAENPPKKYQDIYPLNFDNAHKKIYAEILRVVLFWVQQGVTTFRVDNPHTKPANFWEW
LIAKVHAKHPEVIFLAEAFTRPARLYGLGKVGFSQSYTYFTWKTSKTQLQQFAEEIAEMADVSRPNLFVNTPDILHESLQ
YGGRAMFAIRATLAATMSPVWGVYSGFELFEHEAVAHGSEEYANSEKYELRPRNFDTARAAGNSLEPYLTLLNSIRAQHP
ALQQLRVIDFHPTDNDSILAYSKVDPVTGDTIVVVVNLDPDHAQQATLELDMEALGRDTTESFDVTDLVSGQQFHWGQRN
FIRLDPCANVAHIVAIPPAPNSRKAQLSWRGESDYRN

Specific function: Could be involved in glycogen catabolism [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR013780
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF11896 DUF3416 [H]

EC number: 3.2.1.1

Molecular weight: Translated: 75389; Mature: 75257

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKSTRLAIEDVRPQVSCGRRPSKAVVGEMIPVSALVWREGHDAIAATLVVTDPTGQSS
CCCCCCEEEHHHCCCHHHCCCCCCHHHHHHHCCHHHEEEECCCCEEEEEEEEECCCCCCC
SIAMATKKFEPDRMWASFVPDRPGTWTFRVDAWSDAVATWRHAVTTKIEAGQSPEELYND
CEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHEEECCCCCHHHHHHH
IEHGVALFESAIASAPKAERKTLAPAFTRVINALRSDAPLRTRVAPALSAEITYLLAEHP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHCH
VRELLTRGHTYSISVERTKALYSSWYELFPRSTGGWDDNGQPVHGTFVTTAQALDRVAAM
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHC
GFDTVYFPPIHPIGEINRKGKNNTLIALPGDVGSPWAIGSAEGGHDSVHPRLGTIDDFRA
CCCEEECCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LIARARELNLEVALDLALQAAPDHPWAKSHPEFFTVLADGSIAFAENPPKKYQDIYPLNF
HHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHCCCCCCCC
DNAHKKIYAEILRVVLFWVQQGVTTFRVDNPHTKPANFWEWLIAKVHAKHPEVIFLAEAF
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHH
TRPARLYGLGKVGFSQSYTYFTWKTSKTQLQQFAEEIAEMADVSRPNLFVNTPDILHESL
CCCHHEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHH
QYGGRAMFAIRATLAATMSPVWGVYSGFELFEHEAVAHGSEEYANSEKYELRPRNFDTAR
HHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCHHH
AAGNSLEPYLTLLNSIRAQHPALQQLRVIDFHPTDNDSILAYSKVDPVTGDTIVVVVNLD
HCCCCCCHHHHHHHHHHHCCCHHHHEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEECC
PDHAQQATLELDMEALGRDTTESFDVTDLVSGQQFHWGQRNFIRLDPCANVAHIVAIPPA
CCCCHHHHHEEEHHHHCCCCCCCCCHHHHCCCCCEECCCCCCEEECCCCCEEEEEEECCC
PNSRKAQLSWRGESDYRN
CCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SSKSTRLAIEDVRPQVSCGRRPSKAVVGEMIPVSALVWREGHDAIAATLVVTDPTGQSS
CCCCCEEEHHHCCCHHHCCCCCCHHHHHHHCCHHHEEEECCCCEEEEEEEEECCCCCCC
SIAMATKKFEPDRMWASFVPDRPGTWTFRVDAWSDAVATWRHAVTTKIEAGQSPEELYND
CEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHEEECCCCCHHHHHHH
IEHGVALFESAIASAPKAERKTLAPAFTRVINALRSDAPLRTRVAPALSAEITYLLAEHP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHCH
VRELLTRGHTYSISVERTKALYSSWYELFPRSTGGWDDNGQPVHGTFVTTAQALDRVAAM
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHC
GFDTVYFPPIHPIGEINRKGKNNTLIALPGDVGSPWAIGSAEGGHDSVHPRLGTIDDFRA
CCCEEECCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LIARARELNLEVALDLALQAAPDHPWAKSHPEFFTVLADGSIAFAENPPKKYQDIYPLNF
HHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHCCCCCCCC
DNAHKKIYAEILRVVLFWVQQGVTTFRVDNPHTKPANFWEWLIAKVHAKHPEVIFLAEAF
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHH
TRPARLYGLGKVGFSQSYTYFTWKTSKTQLQQFAEEIAEMADVSRPNLFVNTPDILHESL
CCCHHEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHH
QYGGRAMFAIRATLAATMSPVWGVYSGFELFEHEAVAHGSEEYANSEKYELRPRNFDTAR
HHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCHHH
AAGNSLEPYLTLLNSIRAQHPALQQLRVIDFHPTDNDSILAYSKVDPVTGDTIVVVVNLD
HCCCCCCHHHHHHHHHHHCCCHHHHEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEECC
PDHAQQATLELDMEALGRDTTESFDVTDLVSGQQFHWGQRNFIRLDPCANVAHIVAIPPA
CCCCHHHHHEEEHHHHCCCCCCCCCHHHHCCCCCEECCCCCCEEECCCCCEEEEEEECCC
PNSRKAQLSWRGESDYRN
CCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; cholesteryl-beta-D-glucoside

Specific reaction: H2O + cholesteryl-beta-D-glucoside = D-glucose + cholesterol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10542168 [H]