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The map label for this gene is lacF

Identifier: 15925181

GI number: 15925181

Start: 2335305

End: 2335616

Strand: Reverse

Name: lacF

Synonym: SAV2191

Alternate gene names: 15925181

Gene position: 2335616-2335305 (Counterclockwise)

Preceding gene: 15925182

Following gene: 15925180

Centisome position: 81.14

GC content: 36.22

Gene sequence:

>312_bases
ATGAATAGAGAAGAAGTCCAATTATTAGGTTTTGAAATTGTTGCATTTGCAGGGGATGCACGTTCTAAGTTTTTAGAAGC
ATTGACAGCAGCTCAAGCTGGAGATTTTGCAAAAGCAGATGCATTGATTGAAGAAGGAAACAATTGCATTGCTGAAGCGC
ATAGAGCACAAACAAGCCTGTTAGCTAAAGAAGCGCAAGGTGATGATATTGCATACAGTGTAACGATGATGCATGGTCAA
GACCATTTAATGACAACAATTTTACTGAAAGATTTAATGAAGCATTTATTAGAATTTTATAAAAGAGGGTGA

Upstream 100 bases:

>100_bases
TACAACTGGTTTCAAAAATATTGATGACTTAAATAAAGTATTAAAAGATACTGCGACATCTTGGAAACAAAGAAAATAAG
TATAAAAGGGGATTAATGCT

Downstream 100 bases:

>100_bases
TGACAATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCACGTAACATATATTTA
AGAGCCATCCGTGATGGATT

Product: PTS system, lactose-specific IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEEGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQ
DHLMTTILLKDLMKHLLEFYKRG

Sequences:

>Translated_103_residues
MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEEGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQ
DHLMTTILLKDLMKHLLEFYKRG
>Mature_103_residues
MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEEGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQ
DHLMTTILLKDLMKHLLEFYKRG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain

Homologues:

Organism=Escherichia coli, GI1788031, Length=99, Percent_Identity=34.3434343434343, Blast_Score=65, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTLA_STAAC (Q5HE14)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186993.1
- ProteinModelPortal:   Q5HE14
- SMR:   Q5HE14
- STRING:   Q5HE14
- EnsemblBacteria:   EBSTAT00000009991
- GeneID:   3238568
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL2182
- TIGR:   SACOL2182
- eggNOG:   COG1447
- GeneTree:   EBGT00050000024966
- HOGENOM:   HBG532362
- OMA:   GCIAEAH
- ProtClustDB:   CLSK2518166
- BioCyc:   SAUR93062:SACOL2182-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR003188
- Gene3D:   G3DSA:1.20.58.80
- PIRSF:   PIRSF000699
- TIGRFAMs:   TIGR00823

Pfam domain/function: PF02255 PTS_IIA; SSF46973 Ptrans_IIA

EC number: 2.7.1.69

Molecular weight: Translated: 11370; Mature: 11370

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: ACT_SITE 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEEGNNCIAEAHRAQTSL
CCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKRG
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEEGNNCIAEAHRAQTSL
CCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKRG
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA