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The map label for this gene is opuD [H]

Identifier: 15925175

GI number: 15925175

Start: 2326631

End: 2328193

Strand: Reverse

Name: opuD [H]

Synonym: SAV2185

Alternate gene names: 15925175

Gene position: 2328193-2326631 (Counterclockwise)

Preceding gene: 15925176

Following gene: 15925174

Centisome position: 80.88

GC content: 35.51

Gene sequence:

>1563_bases
ATGAAAAATAAAACAGTAAGTCTAGTGTTTTGGGTATCTTTAGTGATATGTACGATATTTGTTGCTTTTGGAGCAATATT
TCCGAAACAATTAGAAAAACTCACGCAAAACATTACTACATTTATTGCGCTACATTTTTCTTGGTATTATTTATTGCTCG
TTTTAGTTATTTTATTTGTCTGTGTGTATATTTTATTTTCGAGATACGCAAACATTACATTAGGTGAAGAAGGCGAAGAT
CCTGAATTCTCATTGCCGTCTTGGTTTGCTATGTTATTTAGTGCTGGTATGGGAATAGGACTTGTGTTCTGGACAACGGC
AGAACCAATCAGTCATGCCTTCAAATTAACGCCTATTCATAAAGCTGGCACGCAATCTGCAATTAATGATGCAATGCAGT
TTTCATTTTTCCATTGGGGGATTCATGCATGGGCAGTCTATGGTATTGTCGCGCTTGTGTTTGCATATTTTAGCTTTCAT
AAAGGATATCCTGGCTTAGTCAGCGCTACATTGACGCCTTTATTAGGTGAAAAGGCAATGCGTGGACCTTTAGGTGGTGC
CATTGATGTATTGGCAGTCATAGCTACAGTAACAGGCGTTGCTGCAACATTAGGTTTCGGTGCATTGCAAATAAACGAAG
GTTTACATTTTTTATTTAATGTACCGAGTAACTTCACGATGCAAGTAATCTTGATTGTCATTGCAACCATATTATTTACT
TGGTCAGCATGGTCAGGTATCGATAAAGGTATCAAAACGTTGAGTAATATCAACATGTTACTTGCTTTTGTAGTACTTAT
TGGTCTTTTTATTGTTGGGCCAACGCTTTACATTTTAAATACATTTACAAATGGTCTAGGTAATTATATTGCAAATTTCT
TTAGTATGAGTTTACGTATTCCGTCAGGCGGTCAAAAATTCCAATGGTTACAAAATTGGACAATCTTTTACTGGGCATGG
TGGATATCTTGGGCACCATTTGTAGGTATATTTATTGCACGTGTGTCAAAAGGACGTACGATTAAAGAATTTATTCTAGG
TGTACTTTTTGTCCCGGCGTTAGTTTGCTTTATATTCTTTGCGGTATTCGGCGCGTCAGCAATTTACTTACAAGATAATC
ATATTGCCGATATTGCTAAAGCAGCGACAGAAACAGCAACTTTTGCAACGCTACAACATTACCCACTAGGTTTTGTATTA
AGCTTAATTACTTTACTTGTTATTATGATTTTCTTTGTTACATCAGCGGATTCGGCAACTTATGTTTTAGGTATGTTGAG
TTCAAGAGGTGATATTAATCCGAAGTCATTTGTCAAAGTGAGTTGGGGTATCATTATGGCATTATTTGCAATTATCATGA
TCTACACAGGTGGTACTCAAGCGATTCAAAATTTATTAATCATTGCTGCGTTACCATTTTCCGTGGTCATTATAGCGATG
ATATGGTCGCTTTTAAAATCTTTAAGTGAAGAAAAACCTAGAAATAGTAATAAGGTTTTAATTAAACATCGTGACCCAGA
TATTTTAGAATATCGTTCTTCAAAACATATTGACGAAAATTAA

Upstream 100 bases:

>100_bases
AGATGAGGCATTATTAATTGTGTCTATATAAAATTACAAATGATTAAAATGTTGCAGTTATGGGTATGAACTTACCATTA
CACAGAAAGGGTTTGTATTT

Downstream 100 bases:

>100_bases
TTAATAATGAGGTTTTATTTACAAAAACTAGGGAATATATTACTAAATGGTACTTCATCTTATATATTAAAAATAAATTT
AAAGTTATTAATTTATTATG

Product: glycine betaine transporter opuD-like protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKNKTVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Sequences:

>Translated_520_residues
MKNKTVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
>Mature_520_residues
MKNKTVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=520, Percent_Identity=35.7692307692308, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI1788102, Length=451, Percent_Identity=32.3725055432373, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1786224, Length=459, Percent_Identity=29.1938997821351, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57706; Mature: 57706

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKTVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIH
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHEEECCCH
KAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
PSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFF
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSAT
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
YVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKNKTVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIH
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHEEECCCH
KAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
PSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFF
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSAT
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
YVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]