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The map label for this gene is qor [C]

Identifier: 15925176

GI number: 15925176

Start: 2328501

End: 2329508

Strand: Reverse

Name: qor [C]

Synonym: SAV2186

Alternate gene names: 15925176

Gene position: 2329508-2328501 (Counterclockwise)

Preceding gene: 15925177

Following gene: 15925175

Centisome position: 80.93

GC content: 33.73

Gene sequence:

>1008_bases
ATGAAAATGATAGGATTTGAAAAGCCCTTTAAATTGGAAGAAGGAAATTTATTTAAAGTATACGAACAAAGAAAGCCAAC
GCCAGAAAACGATGATATCTTAGTCAAAGTAAATAGTATTAGTGTAAATCCTGTAGATACAAAACAACGTCAAATGAAAG
TCACTCAAGCACCTCGTGTACTTGGGTTTGATGCTATTGGTACTGTTGAAGCTATCGGACCAGACGTTACATTATTTAGT
CCGGGTGACGTTGTATTTTATGCTGGATCGCCAAATAGACAAGGATCAAATGCGACATATCAATTGGTGTCAGAAGCTAT
AGTTGCAAAGGCACCACACAACATAAGTGCTAATGAAGCGGTTAGTCTACCGCTAACTGGTATTACGGCATATGAAACGT
TTTTTGATACATTTAAAATTTCACATAATCCTTCTGAAAATGTAGGTAAATCCGTGTTAATCATTAATGGTGCCGGTGGT
GTAGGCAGTATAGCCACTCAAATTGCTAAACGGTATGGTTTAACTGTCATTACAACAGCATCACGACAAGAAACAACAGA
GTGGTGTGAAAAGATGGGCGCAGATATCGTATTGAATCATAAAGAGGATTTAGTGCGTCAATTTAAAGAAAAAGAAATTC
CATTAGTAGATTATATATTTTGCACTTATAATACAGACTTGTATTACAATACTATGATTGAACTGATAAAACCATTAGGG
CATATTACGACTATCGTAGCTTTTAATGAAGATCAAGATTTAAATGCATTAAAATTAAAAAGTATAACATTTACACATGA
ATTTATGTTTGCGCGTCCAATTCATCGAACGCCTGATATGATTAAACAACATGAGTATTTAGAAGATATTACGAAAAATA
TAGAATTAGGGCATTACCAACCAACAACGACACAAGTTTTTGAAGGTTTATCACCTGAAAACTTATATCAAGCACATCAA
TTGTTGGAAAAACAATCAATGATTGGGAAATTAGTAATTAATATATAG

Upstream 100 bases:

>100_bases
ATATATTCAAATGAAAAAGGAGTCATGATGCTGCGGTATAAGCACACATGGCTTCTATTTTGTATAATAAGAAATAAGTT
CATAAATGAGGTGAAACGAT

Downstream 100 bases:

>100_bases
ATTAGTAAGTAAAGTCGCCTTATGCAGAAAATACTGTATAGGGCGACTTATTTTTTGTGTCTAGCGACTATTGATTCTAA
ACGGAAATATGGCAACTTTA

Product: alginate lyase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNPVDTKQRQMKVTQAPRVLGFDAIGTVEAIGPDVTLFS
PGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIELGHYQPTTTQVFEGLSPENLYQAHQ
LLEKQSMIGKLVINI

Sequences:

>Translated_335_residues
MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNPVDTKQRQMKVTQAPRVLGFDAIGTVEAIGPDVTLFS
PGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIELGHYQPTTTQVFEGLSPENLYQAHQ
LLEKQSMIGKLVINI
>Mature_335_residues
MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNPVDTKQRQMKVTQAPRVLGFDAIGTVEAIGPDVTLFS
PGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIELGHYQPTTTQVFEGLSPENLYQAHQ
LLEKQSMIGKLVINI

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily

Homologues:

Organism=Homo sapiens, GI47519420, Length=332, Percent_Identity=26.5060240963855, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI194239674, Length=207, Percent_Identity=30.9178743961353, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI13236495, Length=207, Percent_Identity=30.9178743961353, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI194239676, Length=180, Percent_Identity=32.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI22538446, Length=207, Percent_Identity=29.951690821256, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI22538444, Length=207, Percent_Identity=29.951690821256, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI24308257, Length=236, Percent_Identity=27.1186440677966, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI28557745, Length=193, Percent_Identity=29.5336787564767, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790485, Length=189, Percent_Identity=33.8624338624339, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507255, Length=235, Percent_Identity=28.936170212766, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71987554, Length=351, Percent_Identity=23.9316239316239, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=188, Percent_Identity=31.3829787234043, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324195, Length=238, Percent_Identity=28.1512605042017, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZDH1_STAAM (P63475)

Other databases:

- EMBL:   BA000017
- RefSeq:   NP_372710.1
- ProteinModelPortal:   P63475
- SMR:   P63475
- STRING:   P63475
- World-2DPAGE:   0002:P63475
- EnsemblBacteria:   EBSTAT00000007215
- GeneID:   1122211
- GenomeReviews:   BA000017_GR
- KEGG:   sav:SAV2186
- eggNOG:   COG0604
- GeneTree:   EBGT00050000023956
- HOGENOM:   HBG753318
- OMA:   TAWEGLF
- ProtClustDB:   CLSK885817
- BioCyc:   SAUR158878:SAV2186-MONOMER
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11695
- TIGRFAMs:   TIGR02817

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N; SSF50129 GroES_like

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 37574; Mature: 37574

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNPVDTKQRQMKVTQAPRV
CCCCCCCCCCEECCCCEEEEEHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCE
LGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEA
ECCCCCCCHHHCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
VSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGGVGSIATQIAKRYGLTVITTA
EECCCCCHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEC
SRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
CCHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIELGHYQ
HEEEEEEECCCCCCCEEEEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNPVDTKQRQMKVTQAPRV
CCCCCCCCCCEECCCCEEEEEHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCE
LGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEA
ECCCCCCCHHHCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
VSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGGVGSIATQIAKRYGLTVITTA
EECCCCCHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEC
SRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
CCHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIELGHYQ
HEEEEEEECCCCCCCEEEEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146