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The map label for this gene is trmB

Identifier: 15924738

GI number: 15924738

Start: 1877471

End: 1878115

Strand: Reverse

Name: trmB

Synonym: SAV1748

Alternate gene names: 15924738

Gene position: 1878115-1877471 (Counterclockwise)

Preceding gene: 15924739

Following gene: 15924737

Centisome position: 65.25

GC content: 31.94

Gene sequence:

>645_bases
ATGAGAGTTCGATACAAACCATGGGCTGAAGATTATTTAAAAGATCATCCTGAATTGGTTGACATGGAAGGTCAACATGC
AGGGGAAATGACTGAATGGTTTGATAAAACGCAACCGATACACATTGAAATTGGTTCGGGTATGGGGCAGTTTATTACAA
CATTAGCTGCTCAAAATCCTCATATTAACTATATTTCAATGGAACGTGAGAAAAGTATCGTTTATAAAGTATTGGATAAA
GTTAAAGAGATGGGTTTAACAAATTTAAAGATTATTTGTAATGACGCCATTGAATTAAATGAATATTTTAAAGACGGCGA
AGTTTCGCGTATTTATTTGAACTTTTCAGATCCATGGCCAAAAAATCGTCATGCAAAACGACGTTTAACGTATCATACGT
TTTTAGCGTTGTATCAACAAATCTTAAATGATGAAGGAGATTTACATTTTAAAACTGATAACAGAGGACTATTTGCCTAC
AGTTTAGAAAGTATGTCTCAATTTGGAATGTATTTTACAAAAATAAATCTAAATTTACATCAAGAAGACGATGGCAGTAA
TATTTTAACAGAATATGAAAAGAAATTTTCGGATAAAGGGTCACGTATTTATCGTATGGAAGCGAAATTCCATTCACAAA
AATAA

Upstream 100 bases:

>100_bases
GGTATGAAGAGCAAAAAAGATATAAGGATATGAACACTTGGCTTAAATTCCTAAATGAAGTTATGAATAGTAACATGTTT
ATTTAAGAGGAGAATAAAAT

Downstream 100 bases:

>100_bases
AACACTTATGAGGGTATGTACACATACAATCATAAGTGTTTTTTCGTACTATAATGGTTCTATAGATAAAGGTTCTATAA
TAAATCGGACTGATGGAAAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDK
VKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK

Sequences:

>Translated_214_residues
MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDK
VKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK
>Mature_214_residues
MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDK
VKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=172, Percent_Identity=30.2325581395349, Blast_Score=90, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_STAA1 (A7X3I5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442324.1
- ProteinModelPortal:   A7X3I5
- SMR:   A7X3I5
- STRING:   A7X3I5
- EnsemblBacteria:   EBSTAT00000004765
- GeneID:   5560682
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1734
- eggNOG:   COG0220
- GeneTree:   EBGT00050000025504
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   SAUR418127:SAHV_1734-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25291; Mature: 25291

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: BINDING 43-43 BINDING 68-68 BINDING 95-95 BINDING 117-117 BINDING 121-121 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNP
CCCCCCCCHHHHHHCCCCEECCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCC
HINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWP
CCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEEEEECCCCC
KNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAYSLESMSQFGMYFTKINLNLH
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHCEEEEEEEEEEE
QEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK
CCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNP
CCCCCCCCHHHHHHCCCCEECCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCC
HINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWP
CCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEEEEECCCCC
KNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAYSLESMSQFGMYFTKINLNLH
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHCEEEEEEEEEEE
QEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK
CCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA