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The map label for this gene is nifS [H]

Identifier: 15898990

GI number: 15898990

Start: 2037536

End: 2038666

Strand: Reverse

Name: nifS [H]

Synonym: SSO2215

Alternate gene names: 15898990

Gene position: 2038666-2037536 (Counterclockwise)

Preceding gene: 15898991

Following gene: 15898988

Centisome position: 68.13

GC content: 35.46

Gene sequence:

>1131_bases
ATGAGATTAAGAGATCCGAGAGAATTTAGGGAAAACGTACCCGTTACCAGAAAATACGTATATTTAAATCATGCATCAGT
GTCACCCACACCTTTACCGTCGTTATTTGAGGCTTACAGATATTTATATGAAGTTGCAAATAGGGGAAGCATAGCTGTTA
ATGAAGAGGAAGAGGATGAACTGTATCACATAAGGTCTAAAATATCCAATTTGGTAGGAGCATACTCAGATGAGATTTCG
CTAATTCCAAATACTAGTTATGGGGTAAACTTAGTTGCACATGGGCTAGAATGGAAGGGAGATGATAATATAGTAACAGA
TAACCTTGAGTTCCCAACTGTAGTGTACCCATTTTTGAAATTAACGAAAAAAGGAGTCAAGATAAATATAGTAGAGACTA
ATCCCTATACCTTTGAGGAAGATATAATATCACATATTGATAAAAATACTAGATTAGTTGCAATAAGCCATGTTAGCTTT
AATACTGGTCTGAAAGTAGATGTTAGAAAAATTGTAAAAGCCGCAAGGGAGAACAATACTCTAGTTCTATTAGATATCAT
ACAGAGTGCTGGTGCAGTCAAAATAAATGTAAAGGAACTTGGTATAGATTTCGCTATTGCTGGAGGATATAAATGGTTAA
TGAGTCCACAAGGATCCGGATTTATCTATGTTAAAAGAGGATTGATAGAAGATCCACCGTTTTATGGATGGAAAACTAGT
GCTGATTACTTGGATTTTAATCCAAATAAGTTTACATTAGAGAAGGGTCCTAGAAGATTTGAAATAGGTACAGTAGATTT
AGCTGCAAACTTATCACTTGCAAAGTCTTGCGAAATAATAGGCGAAAATATGGAATTAATTGAGAGTTCAGTGACGAATC
TTTCCCAATTTGCAATAAGATTAGCAAAGGACCATAGCATGGAGGTAATCACTCCAGAGGATAAGAGAGCTGGAATTGTC
ATAGTAAAGGTTAAAAAACCTAAAGAGATAGCGAAGGAACTATTAAAGGAAAACATAGTTGTGTCGCCAAGAGGAGAAGG
GATAAGGATATCAACGCACTTCTACAATACAGAGGAGGAAGTTCAAAAGACTATTGAGAAAATCTCAGAACTCGAAAGAA
AATTCAACTAG

Upstream 100 bases:

>100_bases
AGTGGTAAAATAGTTAGAAGAGTTTTGAGAGATTTATTATTGGGAAAAGAAATAGATGAAACTATATTGGAAGACCCAGA
AATATTAAAGGAGATCAAAA

Downstream 100 bases:

>100_bases
AATTTTTAACCATATATTTCCCTATATTATTTATGGCCTCTTCAACATCTTCCATAATTCTTACGAGCATACTGTATAAG
ATTTCGTCATCAACATTATC

Product: NifS protein homolog (nifS)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRLRDPREFRENVPVTRKYVYLNHASVSPTPLPSLFEAYRYLYEVANRGSIAVNEEEEDELYHIRSKISNLVGAYSDEIS
LIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLKLTKKGVKINIVETNPYTFEEDIISHIDKNTRLVAISHVSF
NTGLKVDVRKIVKAARENNTLVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIEDPPFYGWKTS
ADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGENMELIESSVTNLSQFAIRLAKDHSMEVITPEDKRAGIV
IVKVKKPKEIAKELLKENIVVSPRGEGIRISTHFYNTEEEVQKTIEKISELERKFN

Sequences:

>Translated_376_residues
MRLRDPREFRENVPVTRKYVYLNHASVSPTPLPSLFEAYRYLYEVANRGSIAVNEEEEDELYHIRSKISNLVGAYSDEIS
LIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLKLTKKGVKINIVETNPYTFEEDIISHIDKNTRLVAISHVSF
NTGLKVDVRKIVKAARENNTLVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIEDPPFYGWKTS
ADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGENMELIESSVTNLSQFAIRLAKDHSMEVITPEDKRAGIV
IVKVKKPKEIAKELLKENIVVSPRGEGIRISTHFYNTEEEVQKTIEKISELERKFN
>Mature_376_residues
MRLRDPREFRENVPVTRKYVYLNHASVSPTPLPSLFEAYRYLYEVANRGSIAVNEEEEDELYHIRSKISNLVGAYSDEIS
LIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLKLTKKGVKINIVETNPYTFEEDIISHIDKNTRLVAISHVSF
NTGLKVDVRKIVKAARENNTLVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIEDPPFYGWKTS
ADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGENMELIESSVTNLSQFAIRLAKDHSMEVITPEDKRAGIV
IVKVKKPKEIAKELLKENIVVSPRGEGIRISTHFYNTEEEVQKTIEKISELERKFN

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=400, Percent_Identity=24.75, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=23.5, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42569; Mature: 42569

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRDPREFRENVPVTRKYVYLNHASVSPTPLPSLFEAYRYLYEVANRGSIAVNEEEEDE
CCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHH
LYHIRSKISNLVGAYSDEISLIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLK
HHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCEECCCCCEEECCCCCCHHHHHHHH
LTKKGVKINIVETNPYTFEEDIISHIDKNTRLVAISHVSFNTGLKVDVRKIVKAARENNT
HHCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCC
LVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIEDPPFYGWKTS
EEEEEEHHCCCEEEEEHHHCCEEEEEECCEEEEECCCCCCEEEEECCCCCCCCCCCCCCC
ADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGENMELIESSVTNLSQFAIR
CCCEECCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAKDHSMEVITPEDKRAGIVIVKVKKPKEIAKELLKENIVVSPRGEGIRISTHFYNTEEE
HHCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCCCCCEEEEEEEECCHHH
VQKTIEKISELERKFN
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLRDPREFRENVPVTRKYVYLNHASVSPTPLPSLFEAYRYLYEVANRGSIAVNEEEEDE
CCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHH
LYHIRSKISNLVGAYSDEISLIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLK
HHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCEECCCCCEEECCCCCCHHHHHHHH
LTKKGVKINIVETNPYTFEEDIISHIDKNTRLVAISHVSFNTGLKVDVRKIVKAARENNT
HHCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCC
LVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIEDPPFYGWKTS
EEEEEEHHCCCEEEEEHHHCCEEEEEECCEEEEECCCCCCEEEEECCCCCCCCCCCCCCC
ADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGENMELIESSVTNLSQFAIR
CCCEECCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAKDHSMEVITPEDKRAGIVIVKVKKPKEIAKELLKENIVVSPRGEGIRISTHFYNTEEE
HHCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCCCCCEEEEEEEECCHHH
VQKTIEKISELERKFN
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]