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The map label for this gene is gatA-3

Identifier: 15898906

GI number: 15898906

Start: 1946372

End: 1947886

Strand: Reverse

Name: gatA-3

Synonym: SSO2122

Alternate gene names: NA

Gene position: 1947886-1946372 (Counterclockwise)

Preceding gene: 15898915

Following gene: 15898903

Centisome position: 65.1

GC content: 39.34

Gene sequence:

>1515_bases
ATGGGAATTAAGTTACCCACATTGGAGGATTTAAGAGAGATTTCTAAGCAATTTAATTTAGATCTAGAGGATGAGGAGTT
AAAATCCTTTTTGCAATTACTAAAACTGCAGTTGGAATCCTATGAGAGGTTAGACTCCTTGCCAGATTATACTCCAAGAG
TTAAGTATCAGAGAACATTAGGTCGACCACCTATGAAAGAGGAGAATCCTTATGGTGCTTTAGTGTGGATAACTTCGATA
AAGGGTAAGGAAGAGGGTAAGTTAAAGGGAAAAAGGATTTGCATTAAGGACAATGTGATGATAGCGGGTATACCTATGCT
TAATGGATCTAAAATGTTAGAAGGTTTTGTCCCGCATATGGATGCTACTGTAGTTTCAAGGATTCTGGATGAGGCTGGAG
AAATCGTAGCTAAGACTACGTGTGAGGACCTATGCTTCTCAGGAGGTAGTCATACATCTTATCCTTGGCCAGTACTAAAT
CCGAGAAATCCAGAGTATATGGCAGGAGGATCATCAAGCGGTAGTGCTGTAGCAGTAGCTTCTGGCTATTGTGATATGGC
TGTTGGAGGAGATCAAGGAGGTTCAATTAGAATTCCCAGTTCGTGGGTTGGCATATACGGACTAAAACCAACTCATGGAC
TAGTTCCATATACCGGCGCTTTTTCCATAGAGCCTACATTAGATCATCTAGGACCTATGGCTAATACGGTAAAGGATGTA
GCATTACTTTTAGAGGTAATAGCAGGGAGGGATGAATTGGATTCAAGACAGCCAGATAGCCTACCTCCACCTCCAGTCAA
ACCCTATTCTAAACTTATTGATGGCGACGTAAAAGATATGAAAGTTGGCATAGTTAAGGAAGGATTTAATTGGTCCAATT
CTGAAAAGGATGTTGATGAGTTAGTATTAGATTCTGCCAAGAAACTAGAAGATTATGGAATAAAAGTAGAGGATACCTCC
ATACCTCTTCATAGGATGGGATTGGATATATGGACTCCAATAGCTATTGAGGGTGCCACAGCAACCATGATATTAGGAAG
TGGTGTAGGATGGGGAAGGAAGGGCTTATTTGAGACTCAAATTGCAGATTTCTTTGGGAACTCCTTAAAATCCAGAGCTA
GAGATTTACCAAACACGGTAAAAGGTGTACTAATGTTAGGCTACTTAATGATAAAGATGTATAACAATAGATACTACGCA
AAGGCAAGAAATTTATCGATTGTGTTAAAGGAAGCTTACGACAGTGCGTTAAGGAAATACGACGCTCTAATAATGCCAAC
AACTCCTATGAAAGCAATGAGGTATAAGAGTGAACCAGGCTTTGATGAATATTTTATAATGGCGCTCGGAATGATAAATA
ATACAGCTCCTTTTGACGTTACTGGACACCCTGCTATGAATATACCAGTGGGATATTCGAATGGTTTACCAGTCGGTCTG
ATGATAATAGGAAGGCATTTTGAAGAAGACAAGGTTCTAAAATTAGCTAATGTATTTGAGAGAATCAAAAAATAA

Upstream 100 bases:

>100_bases
TTCTCCATCCTACGGTTACAAACTCAGATCTGTTAGATACGAAGCATGTAATGCTTCTTTTAAAACTTTTGTTTATAAGA
TTGCCTTACTAAAATATTTT

Downstream 100 bases:

>100_bases
ATTTAATTTTACCCATTCATGTTATGTTGTTTTAACTTCTTTATAAGTGAACCACCAATCAAAGGGCTGACCTAATCTCA
TCTTTGCATCTTTTATCGTT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MGIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTLGRPPMKEENPYGALVWITSI
KGKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLN
PRNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDV
ALLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDELVLDSAKKLEDYGIKVEDTS
IPLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQIADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYA
KARNLSIVLKEAYDSALRKYDALIMPTTPMKAMRYKSEPGFDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVGL
MIIGRHFEEDKVLKLANVFERIKK

Sequences:

>Translated_504_residues
MGIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTLGRPPMKEENPYGALVWITSI
KGKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLN
PRNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDV
ALLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDELVLDSAKKLEDYGIKVEDTS
IPLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQIADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYA
KARNLSIVLKEAYDSALRKYDALIMPTTPMKAMRYKSEPGFDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVGL
MIIGRHFEEDKVLKLANVFERIKK
>Mature_503_residues
GIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTLGRPPMKEENPYGALVWITSIK
GKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNP
RNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDVA
LLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDELVLDSAKKLEDYGIKVEDTSI
PLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQIADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYAK
ARNLSIVLKEAYDSALRKYDALIMPTTPMKAMRYKSEPGFDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVGLM
IIGRHFEEDKVLKLANVFERIKK

Specific function: Enantioselective, active on 2-to 6-carbon aliphatic amides and on many aromatic amides

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=451, Percent_Identity=28.8248337028825, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI195972892, Length=447, Percent_Identity=24.3847874720358, Blast_Score=93, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=26.9503546099291, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17537465, Length=458, Percent_Identity=25.764192139738, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=488, Percent_Identity=28.2786885245902, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=31.0904872389791, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161078093, Length=438, Percent_Identity=26.7123287671233, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=424, Percent_Identity=22.877358490566, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45550774, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648435, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648437, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648441, Length=181, Percent_Identity=29.2817679558011, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648439, Length=181, Percent_Identity=29.2817679558011, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMID_SULSO (P95896)

Other databases:

- EMBL:   AF290611
- EMBL:   Y08256
- EMBL:   AE006641
- PIR:   S74034
- RefSeq:   NP_343511.1
- ProteinModelPortal:   P95896
- SMR:   P95896
- GeneID:   1453628
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2122
- NMPDR:   fig|273057.1.peg.1934
- HOGENOM:   HBG481888
- OMA:   VSIPWHL
- ProtClustDB:   PRK07235
- BioCyc:   SSOL273057:SSO2122-MONOMER
- BRENDA:   3.5.1.4
- InterPro:   IPR000120
- InterPro:   IPR020556
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: =3.5.1.4

Molecular weight: Translated: 55656; Mature: 55525

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 96-96 ACT_SITE 171-171 ACT_SITE 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTL
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHC
GRPPMKEENPYGALVWITSIKGKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHM
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCCHHHHHHHCCCC
DATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNPRNPEYMAGGSSSGSAVAVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEE
SGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDV
CCCEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCCCEECCCCHHHHCHHHHHHHHH
ALLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDE
HHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHCCCHHHEEEEEEECCCCCCCCCHHHHH
LVLDSAKKLEDYGIKVEDTSIPLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQ
HHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCCEEECCCEEEEEEECCCCCCCCCCHHHH
IADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYAKARNLSIVLKEAYDSALRKY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEHHHHHHHHHHHH
DALIMPTTPMKAMRYKSEPGFDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVGL
CCEEECCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEE
MIIGRHFEEDKVLKLANVFERIKK
EEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHC
GRPPMKEENPYGALVWITSIKGKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHM
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCCHHHHHHHCCCC
DATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNPRNPEYMAGGSSSGSAVAVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEE
SGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDV
CCCEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCCCEECCCCHHHHCHHHHHHHHH
ALLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDE
HHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHCCCHHHEEEEEEECCCCCCCCCHHHHH
LVLDSAKKLEDYGIKVEDTSIPLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQ
HHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCCEEECCCEEEEEEECCCCCCCCCCHHHH
IADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYAKARNLSIVLKEAYDSALRKY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEHHHHHHHHHHHH
DALIMPTTPMKAMRYKSEPGFDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVGL
CCEEECCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEE
MIIGRHFEEDKVLKLANVFERIKK
EEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11453462; 8899719; 11427726