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The map label for this gene is hpaA

Identifier: 15898843

GI number: 15898843

Start: 1871635

End: 1873161

Strand: Reverse

Name: hpaA

Synonym: SSO2053

Alternate gene names: NA

Gene position: 1873161-1871635 (Counterclockwise)

Preceding gene: 15898844

Following gene: 15898842

Centisome position: 62.6

GC content: 38.97

Gene sequence:

>1527_bases
ATGGGTTGCCGTGGAGGATATTTCCACGGGTCAATTAAAGGGGTGGTCTGGATGATAAGAAGGGGAACAGATTACATAAA
AAGTATAAAGGAAAACCCACCAGTAGTTTACTATGAAGGAGAAGTAGTAAAGGATGTTACAGAACATTCAGCATTTAAAA
TACCGATATCTACTGTTGCGAAATATTATGATCTACATTGGGATGAGAAATATAAGGAGTACCTAAGGGTTTATAACCTT
GATGTGGGAGAGGAAACTAGCATAAGTTTTCTAAGACCTAAAAACAAGAAAGATTTAGGCAAATTAAGGGATGGCCTAGT
AAAGATATATGATTACTATAGGGGATTCTTCGGTAGAAGTCCAGACTACCTAAACTTATGGACAATGGTATTCTATGCAC
ACGCTGAGGACTACTTTGGAAAACAGTTCGGTTCAAAGTTCATGGAAAATGCGATTGAAATTTACAAAGAAGCTACAAAG
AGAGATTTGTTCTATACCCACGCAATTGTGGCCCCAATGTACGATAGATCTAGACCACCTTCTCAATGGGAAGATCCATA
CATTCAAGTGGGAATAGTTGAGGAAAAACCAGAAGGTGTAGTAGTAAGAGGAGCCGCAATGATATGTACCGCCGGTCCTT
ATGCGGAGATGTTATGGTATCTACCAAATGTCAGAAGAGATAGTGATCCTAGATATGCAATATACTTCTCTATCCCAACA
AACACTAAAGGAGTTAAGTTCTTGTCTAGAAGGGGGTTCCAGCCTAGGGAAGGAGGAGAATTTGAATACCCAATATCTTC
TAGATTTGAGGAATCCGATGCAGTTTTAGTGTTAGATAACGTCCTAATTCCTTGGGAAAGGATAATATTCTTCAAGAAGC
CTGAGTTAATCGAAGACCTAATGTGGCATACCGTTCAGTTAAGGGGATGGTTTAACTGGCACTTCGTAATTCAACATTAT
AGCAGGTTAAAATTCCTTGCTGGTTTAGCTATCGCCATTGCTGAAGCTTCTGGCACCAGTAATTTCATTAACGTTCAAGA
AAAGATAGGTGAGATACTGATATATGTAGCTCTAAATGAGGCAGCCCTCTATGCATCAGTTGAAAGAGCCCAAGAATTGC
CCAATATTACTAGACCAGATCCCTATATTTCCATTTCAGCCAGTCACTTCAACATGAAGACAGTTCCCAGAGCAAATGAG
ATATTAAGGTCAATTAGTGCAGGATCATCAATACCTATACCTGCTGGTATTAAAGATTTTGAAAATCCCGAGGAAAGAAA
ATTATTGGATAAGTACATGGCAATGAAAGGTCTTGATGCCCTAGAAAGAGTTAAGATGTTTAACCTACTGTGGGACGTTA
TAGGATCTGAAGCAGGGATGAGATATGAACAATATGATAGGTTCAGTAGGGGTGATCCCACAATAAGATGGGCTCAAACT
TATACTGAGGTATTCAAGGATAGAAAGCACGAATTCGTCAAATTGGTTAAGGATATAATCGATCAGATGCCTAATCCTAA
AGCTTAA

Upstream 100 bases:

>100_bases
TGGATCCGGATAAGTGGAAACCAATAGTTTGGACCTATGATCAATTAAGGTATAGAAGTGATTTTTGGGGAAGGCCGATT
CCTCAATCTTGGTTAACTGA

Downstream 100 bases:

>100_bases
GTGAACAAAAGTTAACTTAATTTTTTATTTATATTTTTGGAGTTCTTGTTAATGACAATTGGCTTATTTATATCTCATGG
TTCTCCTACAATACTGATTG

Product: 4-hydroxyphenylacetate-3-hydroxylase (hpaA)

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MGCRGGYFHGSIKGVVWMIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRVYNL
DVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATK
RDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPT
NTKGVKFLSRRGFQPREGGEFEYPISSRFEESDAVLVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHY
SRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANE
ILRSISAGSSIPIPAGIKDFENPEERKLLDKYMAMKGLDALERVKMFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQT
YTEVFKDRKHEFVKLVKDIIDQMPNPKA

Sequences:

>Translated_508_residues
MGCRGGYFHGSIKGVVWMIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRVYNL
DVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATK
RDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPT
NTKGVKFLSRRGFQPREGGEFEYPISSRFEESDAVLVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHY
SRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANE
ILRSISAGSSIPIPAGIKDFENPEERKLLDKYMAMKGLDALERVKMFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQT
YTEVFKDRKHEFVKLVKDIIDQMPNPKA
>Mature_507_residues
GCRGGYFHGSIKGVVWMIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRVYNLD
VGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKR
DLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTN
TKGVKFLSRRGFQPREGGEFEYPISSRFEESDAVLVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHYS
RLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEI
LRSISAGSSIPIPAGIKDFENPEERKLLDKYMAMKGLDALERVKMFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTY
TEVFKDRKHEFVKLVKDIIDQMPNPKA

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 59046; Mature: 58915

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCRGGYFHGSIKGVVWMIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVA
CCCCCCCCCCCHHHEEEEECCCCHHHHHHHCCCCEEEECCHHHHHHHHCCEEECCHHHHH
KYYDLHWDEKYKEYLRVYNLDVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRS
HHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRSRPP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPT
CCCCCCCEEEEEEECCCCCEEEECCEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCC
NTKGVKFLSRRGFQPREGGEFEYPISSRFEESDAVLVLDNVLIPWERIIFFKKPELIEDL
CCCHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCEEEECCCCCCHHHEEEECCHHHHHHH
MWHTVQLRGWFNWHFVIQHYSRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNE
HHHEEEEEEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEHHHHHCCEEEEEEECC
AALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGIKDF
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
ENPEERKLLDKYMAMKGLDALERVKMFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEHHHH
YTEVFKDRKHEFVKLVKDIIDQMPNPKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GCRGGYFHGSIKGVVWMIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVA
CCCCCCCCCCHHHEEEEECCCCHHHHHHHCCCCEEEECCHHHHHHHHCCEEECCHHHHH
KYYDLHWDEKYKEYLRVYNLDVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRS
HHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRSRPP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPT
CCCCCCCEEEEEEECCCCCEEEECCEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCC
NTKGVKFLSRRGFQPREGGEFEYPISSRFEESDAVLVLDNVLIPWERIIFFKKPELIEDL
CCCHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCEEEECCCCCCHHHEEEECCHHHHHHH
MWHTVQLRGWFNWHFVIQHYSRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNE
HHHEEEEEEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEHHHHHCCEEEEEEECC
AALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGIKDF
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
ENPEERKLLDKYMAMKGLDALERVKMFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEHHHH
YTEVFKDRKHEFVKLVKDIIDQMPNPKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA